Histological evaluation of hepatoprotective activity of aqueous extract of leaves of corn in acute toxic liver damage

Introduction. Artemisia L. genus species are the promising sources for the manufacturing of drugs with hepatoprotective activity. They are used as appetizing, anthelmintic, bactericidal, choleretic and anti-inflammatory remedies. The pharmacological activity of Artemisia L. species is caused by the presence of different groups of biologically active substances. The aim of the study – to learn the acute toxicity and the effect of Artemisia absinthium and Artemisia vulgaris water-alcohol extracts on the progress of the toxic tetrachloromethane liver damage. Research Methods. The method of preclinical study of drugs safety was used to determine the acute toxicity. The investigation of hepatoprotective activity of Artemisia absinthium and Artemisia vulgaris extracts was performed using the model of acute tetrachloromethane hepatitis. Hepatoprotector of local manufacturer (“Silibor” tablets) was used as the reference drug. Results and Discussion. It was found that intragastric administration of Artemisia absinthium and Artemisia vulgaris extracts at the dose of 6000 mg/kg does not lead to the death of animals. There were no changes in the integral, hematological, biochemical parameters and in the morphological structure of the internal organs of experimental animals. It allows to characterize the extracts at this dose as almost non-toxic ones (V toxicity class, LD50>5000 mg/kg) according to the toxicity classification of substances. The results of Artemisia absinthium and Artemisia vulgaris extracts study indicate that they show the distinct hepatoprotective activity in condition of acute toxic liver damage. They suppress peroxide destructive processes and reduce the evolution of cytolysis syndrome and their effects are no inferior rather than the effect of tablets “Silibor”. Conclusions. The study of acute toxicity of Artemisia absinthium and Artemisia vulgaris extracts after their intragastric administration at the dose of 6000 mg/kg does not lead to the animals death. They were tidy and had the good appetite. The animals reacted adequately to sound and light stimulation. The processes of urination and defecation were unchanged. Breathing disorders and seizures were not observed. The investigated Artemisia absinthium and Artemisia vulgaris extracts demonstrate hepatoprotective activity in condition of acute toxic liver damage. This is proved by decreasing in the intensity of lipid peroxidation and in the toxicity of tetrachloromethane. Biochemical parameters of the animals’ blood and the liver homogenate became responded to the level of intact animals.

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CHRONOPHARMACOLOGICAL FEATURES OF SILIMARIN’S HEPATOPROTECTOCAL ACTIVITY UNDER PARACETAMOL HEPATITIS IN RATS

Likarska sprava ◽

10.31640/jvd.1-2.2020(9) ◽

2020 ◽

pp. 63-71

Author(s):

K. O. Kalko ◽

S. M. Drogovoz ◽

O. Ya. Mishchenko ◽

L. B. Ivantsyk ◽

K. O. Dehtiarova ◽

...

Keyword(s):

Liver Damage ◽

Hepatoprotective Activity ◽

Time Of Day ◽

The State ◽

Acute Liver Damage ◽

Toxic Liver Damage ◽

Antioxidant Protection System ◽

Toxicity Of Drugs ◽

Acute Toxic

Determination of dependence of the efficiency and toxicity of drugs on the time of day or season is one of the most promising areas of modern experimental and clinical pharmacology. The aim of the study. Aim is to identify features of daily rhythms of silymarin influence on the state of antioxidant/prooxidant and cytolytic processes (the leading link in the implementation of hepatoprotective activity) with paracetamol in rats under conditions of acute toxic liver damage. Materials and methods. Chronopharmacological action features of silymarin were studied by use of the generic drug "Karsil" as an example (Sopharma, Bulgaria) with paracetamol in rats under conditions of acute toxic liver damage. The state of the processes of POL-AOS was evaluated by the content of TBA-active products (TBA-AP), the content of recovered glutathione, the activity of SOD, catalase in hepatocytes, and the cytolytic processes by the activity of ALT in serum. Results. There was established a certain chronoarchitectonics of the rhythms of the antioxidant protection system and the activity of the processes of peroxidation in rats under physiological conditions, which was not disturbed by paracetamol on the background of acute liver damage (with the exception of level of acrophase catalase activity). The same dynamics and commonality of rhythms occurred on the background of the use of silymarin. The maximum display of silymarin hepatoprotective activity was observed under conditions of its use in the morning (09.00) and in the evening (21.00). Conclusions. The use of silymarin during periods of maximal display of its hepatoprotective activity, in the morning (09.00) and in the evening (21.00) may be the way to increase its bioavailability. Chronopharmacological features of silymarin activity have been established and should be taken into account when to conduct its further chronopharmacological studies.

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HEPATOPROTECTIVE ACTIVITY OF AQUEOUS EXTRACT OF OXALIS DEBILIS KUNTH AGAINST CCL4 - INDUCED LIVER DAMAGE

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i9.19100 ◽

2017 ◽

Vol 10 (9) ◽

pp. 231

Author(s):

Rojini Athokpam ◽

Meenakshi Bawari ◽

Manabendra Dutta Choudhury

Keyword(s):

Aqueous Extract ◽

Liver Damage ◽

Elevated Serum ◽

Treated Group ◽

Hepatoprotective Activity ◽

Post Treatment ◽

Histopathological Study ◽

Phytochemical Analysis ◽

Ccl4 Intoxication ◽

Pre Treatment

Objective: To evaluate the hepatoprotective activity of aqueous extract of Oxalis debilis Kunth in carbon tetrachloride (CCl4)-induced hepatotoxicity in Swiss albino mice.Methods: Hepatotoxicity was induced by CCl4 30% in olive oil (1 ml/kg intraperitoneally). Mice were treated with aqueous extract of O. debilis at doses of 250 and 500 mg/kg body weight orally for 14 days. There were two groups, pre-treatment (once daily for 14 days before CCl4 intoxication) and post-treatment (2, 6, 24, and 48 hrs after CCl4 intoxication). The observed effects were compared with a known hepatoprotective agent, silymarin.Results: Pre-treatment and post-treatment groups of aqueous extract of O. debilis significantly reduced elevated serum levels of serum transaminases, alkaline phosphatase, and bilirubin and increased the level of total protein as compared to CCl4-treated group. The histopathological study also confirms the hepatoprotection. Preliminary qualitative phytochemical analysis of the plant revealed the presence of phenolic compounds, tannins, flavonoids, and saponins.Conclusion: The results of this study suggest that O. debilis can be used as safe, cheap, and alternative preventive and protective drugs against liver injury. The protective effect observed could be attributed to the presence of various phytochemicals which are responsible for the restoration of liver damage.

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Antioxidant and hepatoprotective activity of aqueous extract of Solanum fastigiatum (false “Jurubeba”) against paracetamol-induced liver damage in mice

Journal of Ethnopharmacology ◽

10.1016/j.jep.2008.08.017 ◽

2008 ◽

Vol 120 (2) ◽

pp. 226-232 ◽

Cited By ~ 44

Author(s):

S.M. Sabir ◽

J.B.T. Rocha

Keyword(s):

Aqueous Extract ◽

Liver Damage ◽

Hepatoprotective Activity

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The chemokine receptor CXCR3 limits injury after acute toxic liver damage

Laboratory Investigation ◽

10.1038/labinvest.2012.48 ◽

2012 ◽

Vol 92 (5) ◽

pp. 724-734 ◽

Cited By ~ 13

Author(s):

Mirko Moreno Zaldivar ◽

Marie-Luise Berres ◽

Hacer Sahin ◽

Andreas Nellen ◽

Daniel Heinrichs ◽

...

Keyword(s):

Liver Damage ◽

Chemokine Receptor ◽

Toxic Liver Damage ◽

Acute Toxic

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Comparative Evaluation of Hepatoprotective Activities of Trigonella foenum-graecum (seeds), Cymbopogon proximus and Acacia nilotica on a model of acute carbon tetrachloride hepatitis in rats

Experimental and Clinical Gastroenterology ◽

10.31146/1682-8658-ecg-187-3-172-177 ◽

2021 ◽

pp. 172-177

Author(s):

Abbas Abdelhamid Mutawakel ◽

V. I. Inchina ◽

T. V. Tarasova ◽

G. G. Varvanina ◽

I. E. Trubitsyna

Keyword(s):

Carbon Tetrachloride ◽

Liver Damage ◽

Blood Cells ◽

Intraperitoneal Administration ◽

Inflammatory Infiltration ◽

Herbal Preparations ◽

Water Extracts ◽

Toxic Liver Damage ◽

Different Doses ◽

Acute Toxic

An experimental study of the beneficial effect of herbal preparations on acute toxic liver damage with carbon tetrachloride (CCl4) in animals was conducted The goal is to Compare the hepatoprotective effect of three water extracts of herbal preparations: (I) Trigonella foenum-graecum (seeds), (II) Cymbopogon proximus, (III) Asasia nilotica Material and methods. To simulate acute toxic liver damage, 50% CCl4 was administered once intraperitonally in doses of 0.6 ml, 0.3 ml, and 0.2 ml. Control groups: K1-1 group intact animals without injections; K2: groups 2, 3, 4. introduced saline solution (FR) without Ssl4 in the volume of 2 ml. Experimental groups: 5, 6, 7 and 8 studied the hepatoprotective properties of aqueous solutions of the studied drugs after intraperitoneal administration of Ssl4 or FR. Researched herbs: I, II, III, in different doses were administered 2 ml of an aqueous solution in breeding 1:10, observation period 5 days, the blood, for research stabilized solution of sodium citrate in the ratio of 1:10, studied the properties of blood cells hematological analyzer PCE-90Vet (USA). The content of total protein, albumin, total cholesterol, triglycerides, urea, creatinine and transaminase activity - ALT, AST-were recorded in the blood serum using an automatic analyzer of the company “ HUMASTAR 600 “(Germany)», Results and discussion. Water extracts of preparations I and II prevented the reduction of red blood cells and platelets, and activated the macrophage pool. All the herb extracts studied prevent platelet reduction, however, but do not bring them up to the indicators of intact animals. The use of solution III prevented the growth of the cytolysis enzyme-ALT. All studied extracts in acute carbon tetrachloride intoxication. they warned of an increase in hypertriglyceremia. The introduction of water extracts II, III in the 1:10 ratio prevented the development of necrotic changes in hepatocytes, inflammatory infiltration, manifestations of fatty dystrophy, and preserved the architectonics of the liver lobule.

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Hepatoprotector activity of iridoid glycosides with respect to heliotrine-induced acute toxic liver damage in mice

Pharmaceutical Chemistry Journal ◽

10.1007/bf02510088 ◽

1999 ◽

Vol 33 (8) ◽

pp. 410-412 ◽

Cited By ~ 2

Author(s):

V. N. Syrov ◽

A. N. Nabiev ◽

Z. A. Khushbaktova ◽

U. V. Zakhidov ◽

M. S. Maksudov ◽

...

Keyword(s):

Liver Damage ◽

Iridoid Glycosides ◽

Hepatoprotector Activity ◽

Toxic Liver Damage ◽

Acute Toxic

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Hepatoprotective Effects of Aqueous Extract of Opilia celtidifolia (Opiliaceae) Leaves against Ethanol-Induced Liver Damage in Rats

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2020/6297475 ◽

2020 ◽

Vol 2020 ◽

pp. 1-8

Author(s):

A. P. Amang ◽

E. Kodji ◽

C. Mezui ◽

M. P. Baane ◽

G. T. Siwe ◽

...

Keyword(s):

Aqueous Extract ◽

Liver Damage ◽

Protective Action ◽

Hepatoprotective Activity ◽

Negative Control ◽

Control Group ◽

Phytochemical Analysis ◽

Hepatocellular Injury ◽

Control Groups ◽

Histological Sections

Objective. Opilia celtidifolia is a plant used in Cameroonian ethnomedicine to cure jaundice. The aim of this study was to evaluate the hepatoprotective activity of O. celtidifolia leaves aqueous extract in rats subjected to ethanol-induced liver damage. Material and Methods. 36 rats including 18 males and 18 females were divided into 6 groups of 3 males and 3 females each, namely, 3 control groups (normal, negative, positive) and 3 test groups. The normal and negative control groups were given distilled water (5 ml/kg), the positive control group received silymarin (100 mg/kg), and the test groups were given O. celtidifolia extract at the doses of 100, 200, and 400 mg/kg. All groups, except the normal control, received concomitantly and daily 40% ethanol (4 g/kg) for 3 weeks to induce hepatotoxicity. Biochemical parameters such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), bilirubin, and lipid profile (total cholesterol, HDL, LDL, and triglycerides) were evaluated. Histological sections of the liver, kidneys, and lungs were examined. Qualitative and quantitative phytochemical analysis of the extract were carried out. Results. The groups treated with the extract at the doses of 200 and 400 mg/kg showed a significant decrease ( p < 0.001 ) of transaminases (ALT and AST), ALP, and bilirubin compared with the negative control. These results were confirmed by observation of histological sections of the liver that confirmed protective action of extract against ethanol-induced hepatocellular injury. Conclusion. O. celtidifolia possesses hepatoprotective properties that could be related to its high content of tannins and saponins in the leaves aqueous extract.

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Hepatoprotective activity of ethanolic and aqueous extract of Turnera aphrodisiaca leaves against CCl4-induced liver injury in rats

International Journal of Basic & Clinical Pharmacology ◽

10.18203/2319-2003.ijbcp20193174 ◽

2019 ◽

Vol 8 (8) ◽

pp. 1758

Author(s):

Naveen Kumar Bathula ◽

Bimalendu Choudhary

Keyword(s):

Alkaline Phosphatase ◽

Superoxide Dismutase ◽

Aqueous Extract ◽

Liver Damage ◽

Hepatoprotective Activity ◽

Serum Markers ◽

Hepatoprotective Agent ◽

Histopathological Studies ◽

The Impact ◽

Dose Dependent

Background: The botanical Latin name of the plant, Turnera aphrodisiaca, describes its ancient use as an aphrodisiac.Methods: The aim of the present study is to evaluate the protective effect of ethanolic and aqueous extract of Turnera aphrodisiaca leaves against carbon tetrachloride (CCl4)-induced liver damage in male Wistar rats.Results: Administration with ethanolic and aqueous extract of Turnera aphrodisiaca leaves (200 and 400 mg/kg) for 7 days significantly reduced the impact of CCl4 toxicity on the serum markers of liver damage, aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase in a dose dependent matter. In addition, treatment of both the extracts resulted in markedly increased the levels of superoxide dismutase and catalase enzymes in rats. The histopathological studies in the liver of rats also supported that both extracts markedly reduced the toxicity of CCL4 and preserved the histoarchitecture of the liver tissue to near normal.Conclusion: Thus, the results suggest that ethanolic and aqueous extract of Turnera aphrodisiaca leaves acts as a potent hepatoprotective agent against CCl4 induced hepatotoxicity in rats.

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