Humoral Immune Response During Coronary Artery Bypass Grafting

Circulation ◽  
2000 ◽  
Vol 102 (suppl_3) ◽  
Author(s):  
Anno Diegeler ◽  
Nicolas Doll ◽  
Thomas Rauch ◽  
Dorothea Haberer ◽  
Thomas Walther ◽  
...  
Keyword(s):  
Immune Response ◽  
Humoral Immune Response ◽  
Operative Approach ◽  
Surgical Trauma ◽  
Off Pump ◽  
Humoral Immune ◽  
Tnf Α ◽  
Significant Difference ◽  
Group A ◽  
Anti Inflammatory

Background —The introduction of limited approaches to the heart and the avoidance of cardiopulmonary bypass (CPB) aim to reduce the invasiveness of CABG by decreasing the systemic release of inflammatory cytokines, such as tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-8, as well as the anti-inflammatory agent IL-10. This study compares the humoral immune response in patients undergoing CABG with standard, minimally invasive, and “off-pump” techniques. Methods and Results —Thirty patients were divided into 3 operative groups: full sternotomy approach plus CPB (group A); full sternotomy approach, off pump (group B); and limited left anterior thoracotomy, off pump (group C). Plasma levels of TNF-α receptors p55 and p75, IL 6, IL-8, and IL-10 were taken at baseline, during CPB, and at 4, 24, and 48 hours and 6 days after surgery. A significant increased release of activated complement factors C5a and C3d, IL-8, and IL-10 was observed in patients subjected to CPB (group A) during the initial period and for a short time after perfusion ( P <0.05). TNF-α receptors p55 and p75 showed a prolonged elevation (up to 48 hours) in the CPB group compared with the 2 off-pump groups. IL-6 showed no different release among the 3 surgical groups throughout the entire period. There was no significant difference in any parameter measured in relation to the type of operative approach. Conclusions —There is an inflammatory, as well as an anti-inflammatory, response during CABG that is related to the general surgical trauma. The release of immune mediators is enhanced by the use of CPB during various perioperative and postoperative phases. The type of operative approach did not influence this immune response.

scholarly journals Pregnancy level of estradiol attenuated virus-specific humoral immune response in H5N1-infected female mice despite inducing anti-inflammatory protection

2019 ◽  
Vol 8 (1) ◽  
pp. 1146-1156
Author(s):  
Courtney L. Finch ◽  
Anding Zhang ◽  
Martina Kosikova ◽  
Toshiaki Kawano ◽  
Marcela F. Pasetti ◽  
...  
Keyword(s):  
Immune Response ◽  
Humoral Immune Response ◽  
Infected Female ◽  
Female Mice ◽  
Humoral Immune ◽  
Attenuated Virus ◽  
Anti Inflammatory

scholarly journals Anti-Inflammatory Activities of Captopril and Diuretics on Macrophage Activity in Mouse Humoral Immune Response

2021 ◽  
Vol 22 (21) ◽  
pp. 11374
Author(s):  
Paweł Bryniarski ◽  
Katarzyna Nazimek ◽  
Janusz Marcinkiewicz
Keyword(s):  
Immune Response ◽  
B Cells ◽  
Humoral Immune Response ◽  
Humoral Response ◽  
Peritoneal Macrophages ◽  
Surface Markers ◽  
Immunomodulatory Effects ◽  
Humoral Immune ◽  
Macrophage Activity ◽  
Anti Inflammatory

Hypertension is accompanied by the over-activation of macrophages. Diuretics administered alone or in combination with hypotensive drugs may have immunomodulatory effects. Thus, the influence of tested drugs on mouse macrophage-mediated humoral immunity was investigated. Mice were treated intraperitoneally with captopril (5 mg/kg) with or without hydrochlorothiazide (10 mg/kg) or furosemide (5 mg/kg) by 8 days. Mineral oil-induced peritoneal macrophages were harvested to assess the generation of cytokines in ELISA, and the expression of surface markers was analyzed cytometrically. Macrophages were also pulsed with sheep red blood cells (SRBC) and transferred to naive mice for evaluation of their ability to induce a humoral immune response. Tested drugs increase the expression of surface markers important for the antigen phagocytosis and presentation. SRBC-pulsed macrophages from mice treated with captopril combined with diuretics increased the secretion of antigen-specific antibodies by recipient B cells, while macrophages of mice treated with hydrochlorothiazide or furosemide with captopril increased the number of antigen-specific B cells. Tested drugs alter the macrophage secretory profile in favor of anti-inflammatory cytokines. Our results showed that diuretics with or without captopril modulate the humoral response by affecting the function of macrophages, which has significant translational potential in assessing the safety of antihypertensive therapy.

scholarly journals Effect of Probiotics on the Performance and Intestinal Health of Broiler Chickens Infected with Eimeria tenella

Vaccines ◽  
2022 ◽  
Vol 10 (1) ◽  
pp. 97
Author(s):  
Muhammad Mohsin ◽  
Ziping Zhang ◽  
Guangwen Yin
Keyword(s):  
Immune Response ◽  
Antioxidant Enzymes ◽  
Tight Junction ◽  
Humoral Immune Response ◽  
Eimeria Tenella ◽  
Serum Chemistry ◽  
Tight Junction Proteins ◽  
Humoral Immune ◽  
Significant Difference ◽  
Junction Proteins

Coccidiosis is an important parasitic disease of poultry with great economic importance. Due to drug resistance issues, the study was conducted to investigate how probiotics (Lactobacillus plantarum or L. plantarum) affected oocysts per gram of feces (OPG), fecal scores, feed conversion ratio (FCR), immunomodulatory effect in terms of the cell-mediated and humoral immune response. Serum chemistry (ALT, AST, LDH, and creatinine) was measured in different treated chicken groups. mRNA expression levels of antioxidant enzymes (SOD 1 and CAT), peptide transporter 1 (PepT 1), and tight junction proteins (ZO and CLDN 1) were also examined in chicken groups infected with Eimeria tenella (E. tenella). Chickens supplemented with L. plantarum 1 × 108 CFU (colony-forming unit) showed an improved cell-mediated and humoral immune response, compared with the control group (p < 0.05). Probiotics also enhanced the performance of antioxidant enzymes, PepT 1, and tight junction proteins, and improved serum chemistry (AST, ALT, and LDH), compared with control-infected, non-medicated chickens. However, no significant difference (p > 0.05) was observed in CLDN 1 expression level and creatinine in all treated chicken groups. These findings demonstrated that probiotics supplementation in the feed can protect the birds against E. tenella infection.

scholarly journals Humoral Response to Microbial Biomarkers in Rheumatoid Arthritis Patients

2021 ◽  
Vol 10 (21) ◽  
pp. 5153
Author(s):  
Seyedesomaye Jasemi ◽  
Gian Luca Erre ◽  
Maria Luisa Cadoni ◽  
Marco Bo ◽  
Leonardo A. Sechi
Keyword(s):  
Rheumatoid Arthritis ◽  
Immune Response ◽  
Humoral Immune Response ◽  
Humoral Response ◽  
Human Endogenous Retrovirus ◽  
Serum Samples ◽  
Humoral Immune ◽  
Bivariate Correlation ◽  
Microbial Biomarkers ◽  
Significant Difference

Background/Objective: Chronic humoral immune response against multiple microbial antigens may play a crucial role in the etiopathogenesis of rheumatoid arthritis (RA). We aimed to assess the prevalence and magnitude of antibody response against various bacterial and viral immunogen peptides in the sera of RA patients compared with the general population. Methods: Polyclonal IgG antibodies (Abs) specific for peptides derived from Porphyromonas gingivalis (RgpA, Kpg), Aggregatibacter actinomycetemcomitans (LtxA1, LtxA2), Mycobacterium avium subsp. paratuberculosis (MAP4027), Epstein–Barr virus (EBNA1, EBVBOLF), and human endogenous retrovirus (HERV-W env-su) were detected by ELISA in serum samples from 148 consecutive RA patients and 148 sex and age-matched healthy controls (HCs). In addition, the presence of a relationship between the positivity and the titer of antibodies and RA descriptors was explored by bivariate correlation analysis. Results: RA patients exhibit a higher prevalence of humoral immune response against all tested peptides compared to HCs with a statically significant difference for MAP4027 (30.4% vs. 10.1%), BOLF (25.7% vs. 8.1%), RgpA (24.3% vs. 9.4%), HERV W-env (20.3% vs. 9.4%), and EBNA1 (18.9% vs. 9.4%) peptides. Fifty-three (35.8%) out of 148 RA serum and 93 (62.8%) out of 148 HCs were negative for all pathogen-derived peptides. There was a significant correlation between OD values obtained by ELISA test against all peptides (p < 0.0001). We also found an increased titer and prevalence of Abs against LtxA1 and LtxA2 in seropositive vs. seronegative RF (p = 0.019, p = 0.018). Conclusion: This study demonstrates a significantly increased humoral response against multiple pathogens in patients with RA and implies that they could be an important factor in the pathogenesis of the disease. Therefore, the role of each individual pathogen in RA needs to be further investigated.

Expression and Clinical Significance of Sperm-Associated Antigen 9 in Patients With Endometrial Carcinoma

2012 ◽  
Vol 22 (1) ◽  
pp. 87-93 ◽  
Author(s):  
Peng Yu ◽  
Lei Yan ◽  
Hui Zhang ◽  
Xiaoyan Lin ◽  
Xingbo Zhao
Keyword(s):  
Immune Response ◽  
Endometrial Cancer ◽  
Endometrial Carcinoma ◽  
Humoral Immune Response ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Humoral Immune ◽  
Significant Difference ◽  
New Type ◽  
Grade 3

ObjectiveTo investigate the expression and humoral immune response of sperm-associated antigen 9 (SPAG9) in endometri al carcinoma.MethodsSperm-associated antigen 9 gene expression levels were evaluated in endometrial carcinoma, endometrial hyperplasia, adjacent tissues, and normal endometrial tissues by reverse transcriptase-polymerase chain reaction, immunohistochemistry, and Western blot. Sperm-associated antigen 9 concentration in serum samples from 10 healthy women, 20 women with benign diseases, and 50 women with endometrial carcinoma was detected by enzyme-linked immunosorbent assay.Results(1) Sperm-associated antigen 9 antibodies were detected in approximately 72% of patients with endometrial cancer but not in healthy controls. (2) A significant difference has been found among pathological types and degrees (P < 0.05), and it was also found to be expressed in transferred lymph nodes. (3) Sperm-associated antigen 9 serum concentration (ng/mL) of patients with endometrial carcinoma is significantly higher than those of the healthy group (P < 0.05). Patients harboring grade 3 endometrial carcinoma were found to have significantly higher SPAG9 concentrations than those of grade 1/grade 2 (P = 0.003).ConclusionsSPAG9 is positively expressed in endometrial cancer, and with a high humoral immune response in patients. It may serve as a new type of endometrial cancer markers for early detection, diagnosis and treatment.

scholarly journals Selected parameters of immune response in pregnant sows vaccinated against Trueperella pyogenes

2014 ◽  
Vol 58 (1) ◽  
pp. 17-21 ◽  
Author(s):  
Krzysztof Kostro ◽  
Urszula Lisiecka ◽  
Andrzej Żmuda ◽  
Krzysztof Niemczuk ◽  
Krzysztof Stojecki ◽  
...  
Keyword(s):  
Immune Response ◽  
T Lymphocytes ◽  
Humoral Immune Response ◽  
Final Stage ◽  
Economic Losses ◽  
Surface Markers ◽  
Humoral Immune ◽  
Trueperella Pyogenes ◽  
Tnf Α ◽  
Immune Potential

Abstract Expression of CD4, CD8, and CD25 surface markers on T lymphocytes and levels of IFNγ, IL-10, and TNF-α in colostrum and milk were determined in sows vaccinated against Trueperella pyogenes in the final stage of pregnancy. The autovaccine, prepared from Trueperella pyogenes, administered twice to pregnant sows six and three weeks before the anticipated delivery significantly increased the percentages of TCD4+, TCD8+, and TCD25+ as well as levels of IFNγ, TNF-α, and IL-10 in colostrum and milk. The enhanced immune potential of colostrum effectively protected the piglets against T. pyogenes infections during weaning and thus reduced the economic losses on the particular farm concerned, where T. pyogenes infections occur endemically. Knowledge of the profile of cellular and humoral immune response in colostrum and milk of vaccinated sows will enable the design of a T. pyogenes infection prophylactic programme for suckling pigs and weaners, which are most susceptible to infections.

scholarly journals Study on the Course of Cryptosporidium baileyi Infection in Chickens Treated with interleukin-1 or Indomethacin

1999 ◽  
Vol 47 (2) ◽  
pp. 207-216 ◽  
Author(s):  
S. Hornok ◽  
Z. Széll ◽  
Tatjana A. Shibalova ◽  
I. Varga
Keyword(s):  
Immune Response ◽  
Skin Test ◽  
Humoral Immune Response ◽  
Interleukin 1 ◽  
Immunological Parameters ◽  
Humoral Immune ◽  
Significant Difference ◽  
Humoral Responses ◽  
Cellular Immune ◽  
Cryptosporidium Baileyi

The effects exerted by human recombinant interleukin-1β (hrIL-1β) and the prostaglandin inhibitor indomethacin on the course of Cryptosporidium baileyi infection in chickens were studied. Daily oocyst shedding was monitored by a quantitative method throughout the experiment. Humoral immune response to C. baileyi was assessed by ELISA at 3 weeks of age while the level of cellular immune response to phytohaemagglutinin-P (PHA-P) by a skin test at 23 days of age. Parenteral application of hrIL-1b decreased oocyst shedding to 62%, but the infection ran a similar course in treated and control birds. The PHA-P skin test demonstrated increased cellular immune reaction in chickens receiving IL-1b, but there was no significant difference in the humoral responses of the two groups as detected by ELISA. On the other hand, indomethacin mixed to the feed lessened oocyst shedding to 13.7% and also shortened its duration. Immunological parameters as reflected by PHA-P skin test and ELISA results indicated enhanced cellular but unaltered humoral immune response. These data suggest that the sys- temic application of interleukin-1 can induce partial protection against C. baileyi in chickens and that prolonged, abundant oocyst shedding is due to an indometha- cin-sensitive immunodepression via the prostaglandin pathway.

scholarly journals SARS-CoV-2 Infection Elicits A Greater Humoral Immune Response In The Second Wave Than The First Wave In Morocco

2021 ◽  
Author(s):  
Sayeh Ezzikouri ◽  
Hind Majidi ◽  
Soad Redwane ◽  
Nadia Meziane ◽  
Abdellatif Zahir ◽  
...  
Keyword(s):  
Immune Response ◽  
Humoral Immune Response ◽  
Blood Donors ◽  
Response Dynamics ◽  
Humoral Immune ◽  
Age Related ◽  
Significant Difference ◽  
One Year ◽  
Different Strains ◽  
Second Wave

Abstract Purpose: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the ongoing pandemic of coronavirus disease 2019 (COVID-19). One year has passed since the first COVID-19 case, and concerns have emerged regarding new variants, meaning that characterizing antibody-mediated immune response dynamics is of paramount importance. Here, we evaluated the humoral immune response against SARS-CoV-2 in subjects during the first and second waves of the pandemic in Morocco. Methods: We assessed humoral immune response in samples from 94 seropositive individuals in the first wave (February to August 2020) and 596 seropositive individuals in the second wave (December 2020 to January 2021). Plasma samples were collected from volunteer blood donors and their levels of serum IgG to SARS-CoV-2 nucleoprotein (NP) were determined using architect SARS-CoV-2 IgG chemiluminescence microparticle immunoassay.Results: Our results revealed an increase of humoral immunity during the second wave than first wave (3.897 ± 0.079 vs. 2.842 ± 0.153, respectively, p<0.0001). Notably, we found an age-related gradient in antibody level, with higher antibody index in subjects at 45 years old and above (p= 0.01451). However, no significant difference was found according to gender (p= 0.8629). Conclusions: Our data highlighted an important issue regarding antibody-mediated immune response against SARS-CoV-2 infection during the second wave and this issue might have arisen due to the dynamics of different strains circulating during the progress of the pandemic.

scholarly journals SARS-CoV-2 Infection Elicits a Greater Humoral Immune Response in the Second Wave Than the First Wave

2021 ◽  
Author(s):  
Sayeh Ezzikouri ◽  
Hind Majidi ◽  
Soad Redwane ◽  
Nadia Meziane ◽  
Abdellatif Zahir ◽  
...  
Keyword(s):  
Immune Response ◽  
Humoral Immune Response ◽  
Blood Donors ◽  
Response Dynamics ◽  
Humoral Immune ◽  
Age Related ◽  
Significant Difference ◽  
One Year ◽  
Different Strains ◽  
Second Wave

Abstract PurposeSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the ongoing pandemic of coronavirus disease 2019 (COVID-19). One year has passed since the first COVID-19 case, and concerns have emerged regarding new variants, meaning that characterizing antibody-mediated immune response dynamics is of paramount importance. Here, we evaluated the humoral immune response against SARS-CoV-2 in subjects during the first and second waves of the pandemic in Morocco. MethodsWe assessed humoral immune response in samples from 94 seropositive individuals in the first wave (February to August 2020) and 596 seropositive individuals in the second wave (December 2020 to January 2021). Plasma samples were collected from volunteer blood donors and their levels of serum IgG to SARS-CoV-2 nucleoprotein (NP) were determined using architect SARS-CoV-2 IgG chemiluminescence microparticle immunoassay.ResultsOur results revealed an increase of humoral immunity during the second wave than first wave (3.897 ± 0.079 vs. 2.842 ± 0.153, respectively, p<0.0001). Notably, we found an age-related gradient in antibody level, with higher antibody index in subjects at 45 years old and above (p= 0.01451). However, no significant difference was found according to gender (p= 0.8629). ConclusionsOur data highlighted an important issue regarding antibody-mediated immune response against SARS-CoV-2 infection during the second wave and this issue might have arisen due to the dynamics of different strains circulating during the progress of the pandemic.

Sign in / Sign up

Export Citation Format

Share Document