Abstract 17095: Association of Cigarette and Electronic Cigarette Use Behaviors With Levels of Inflammatory and Oxidative Stress Biomarkers Among United States Adults, Path 2013-2014

Introduction: Electronic cigarette (e-cigarette) use has increased rapidly in recent years; however, the cardiovascular risks of e-cigarettes remain unclear. Using data from the Population Assessment of Tobacco and Health Survey, this study assesses the cross-sectional association of cigarette and e-cigarette use behaviors with markers of inflammation (high-sensitivity C-reactive protein [hsCRP], soluble intercellular adhesion molecule [sICAM], interleukin-6 [IL-6], fibrinogen), oxidative stress (8-isoprostane [F2PG2a]), and nicotine exposure (cotinine). Methods: We analyzed data from adults (18+ years) who provided urine and blood specimens at wave 1 (2013-2014). Biomarkers were examined as continuous and categorical variables using the clinical cut points of ≥3 mg/L for hsCRP >10 ng/mL for cotinine, and the upper quartiles of fibrinogen (≥381mg/dL), IL-6 (≥2.32 pg/mL), sICAM (≥288.77 ng/mL), and 8-isoprostane (≥788 pg/mL). Progressively adjusted negative binomial and generalized structural equation models were used, as well as sensitivity checks to assess the robustness of associations. Results: Of the 7,019 participants (58.6% non-users, 1.8% current vapers, 29.7% current smokers, 9.9% dual users), 67.2% had ≥1 high inflammatory or oxidative stress marker. Current vapers had increased risk of high hsCRP (IRR 1.28; 95% CI,1.02-1.59) and sICAM (IRR 2.01; 95% CI,1.42-2.85) compared to non-users. Relative to current smokers, current vapers who were former smokers had lower risk of having ≥1 high inflammatory or oxidative stress marker (IRR 0.80; 95% CI,0.69-0.94). Dual users had higher levels of all 5 inflammatory and oxidative stress markers compared to never smokers in both continuous and categorical models, with similar levels compared to current smokers. Current smokers and dual users had higher levels of cotinine compared to exclusive vapers. Conclusions: This study suggests that current vapers may have lower levels of inflammatory and oxidative stress markers compared to current smokers, but only after complete cessation of combustible cigarettes. Our findings strengthen the need for longitudinal studies investigating the association of vaping with markers of cardiovascular risk, particularly among dual users.

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Aberrations in Oxidative Stress Markers in Amyotrophic Lateral Sclerosis: A Systematic Review and Meta-Analysis

Oxidative Medicine and Cellular Longevity ◽

10.1155/2019/1712323 ◽

2019 ◽

Vol 2019 ◽

pp. 1-9 ◽

Cited By ~ 12

Author(s):

Zihao Wang ◽

Zhile Bai ◽

Xiaoyan Qin ◽

Yong Cheng

Keyword(s):

Oxidative Stress ◽

Meta Analysis ◽

Density Lipoprotein ◽

Protein Product ◽

Oxidative Stress Marker ◽

Oxidative Stress Markers ◽

Stress Marker ◽

Stress Markers ◽

Als Patients ◽

Lateral Sclerosis

Oxidative stress has been reported to be involved in the onset and development of amyotrophic lateral sclerosis (ALS). Data from clinical studies have highlighted increased peripheral blood oxidative stress markers in patients with ALS, but results are inconsistent. Therefore, we quantitatively pooled data on levels of blood oxidative stress markers in ALS patients from the literature using a meta-analytic technique. A systematic search was performed on PubMed and Web of Science, and we included studies analyzing blood oxidative stress marker levels in patients with ALS and normal controls. We included 41 studies with 4,588 ALS patients and 6,344 control subjects, and 15 oxidative stress marker levels were subjected to random-effects meta-analysis. The results demonstrated that malondialdehyde (Hedges’ g, 1.168; 95% CI, 0.812 to 1.523; P<0.001), 8-hydroxyguanosine (Hedges’ g, 2.194; 95% CI, 0.554 to 3.835; P=0.009), and Advanced Oxidation Protein Product (Hedges’ g, 0.555; 95% CI, 0.317 to 0.792; P<0.001) levels were significantly increased in patients with ALS when compared with control subjects. Uric acid (Hedges’ g, -0.798; 95% CI, -1.117 to -0.479; P<0.001) and glutathione (Hedges’ g, -1.636; 95% CI, -3.020 to -0.252; P=0.02) levels were significantly reduced in ALS patients. In contrast, blood Cu, superoxide dismutase, glutathione peroxidase, ceruloplasmin, triglycerides, total cholesterol, low-density lipoprotein, high-density lipoprotein, coenzyme-Q10, and transferrin levels were not significantly different between cases and controls. Taken together, our results showed significantly increased blood levels of 8-hydroxyguanosine, malondialdehyde, and Advanced Oxidation Protein Product and decreased glutathione and uric acid levels in the peripheral blood of ALS patients. This meta-analysis helps to clarify the oxidative stress marker profile in ALS patients, supporting the hypothesis that oxidative stress is a central component underpinning ALS pathogenesis.

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Adiposity and Fat Distribution in relation to Inflammation and Oxidative Stress in a Relatively Lean Population of Chinese Women

Disease Markers ◽

10.1155/2013/437076 ◽

2013 ◽

Vol 34 (4) ◽

pp. 279-293 ◽

Cited By ~ 5

Author(s):

Sheng-Hui Wu ◽

Xiao-Ou Shu ◽

Wong-Ho Chow ◽

Yong-Bing Xiang ◽

Xianglan Zhang ◽

...

Keyword(s):

Oxidative Stress ◽

Chinese Women ◽

Plasma Concentrations ◽

Anthropometric Measurements ◽

Oxidative Stress Marker ◽

Oxidative Stress Markers ◽

Anthropometric Measures ◽

Stress Markers ◽

Markers Of Inflammation ◽

And Oxidative Stress

Objectives: This study evaluated associations of various anthropometric measures of adiposity with a panel of inflammatory and oxidative stress markers in a relatively lean population of Chinese women.Methods: This analysis included 1,005 Chinese women aged 40–70 years. Plasma concentrations of inflammatory and oxidative stress markers were measured. Anthropometric measurements were taken by trained interviewers.Results: Body mass index (BMI), waist circumference (WC), and waist-to-height ratio (WHtR) were all positively and linearly associated with the inflammatory markers, CRP, TNF-α, soluble TNF-receptor 1 (sTNF-R1), and IL-6. A significant positive association of these measures of adiposity with the oxidative stress marker F2-IsoP-M, a metabolite of F2-IsoPs, but with not F2-IsoPs was found. Differences in biomarkers between extreme quartiles of anthropometric measurements varied widely, ranging from 9.7% for sTNF-R1 to 162.0% for CRP. For each specific biomarker, various anthropometric measurements exhibited similar ability to explain variations in the biomarker, with the biggest partial r2(11%) observed for CRP.Conclusions: This study suggests that both general adiposity (measured by BMI) and central adiposity (measured by WC and WHtR) are positively and similarly associated with various markers of inflammation and oxidative stress in relatively lean Chinese women. The metabolite F2-IsoP-M of F2-IsoPs may be a better marker ofin vivooxidative stress than its parent compounds.

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Oxidative Stress Levels Are Correlated with Disease Progression and Iron Overload in MDS Patients with Excess Blasts

Blood ◽

10.1182/blood.v128.22.5532.5532 ◽

2016 ◽

Vol 128 (22) ◽

pp. 5532-5532

Author(s):

Naomi Shimizu ◽

Yasuhiro Watanabe ◽

Noriko Ban ◽

Takashi Yamaguchi ◽

Hidekazu Hasunuma ◽

...

Keyword(s):

Oxidative Stress ◽

Flow Cytometry ◽

Iron Overload ◽

Disease Progression ◽

Peripheral Blood ◽

Research Funding ◽

Oxidative Stress Marker ◽

Oxidative Stress Markers ◽

Stress Marker ◽

Stress Markers

Abstract Introduction : Oxidative stress is closely related to iron overload in myelodysplastic syndrome (MDS) and induces DNA damage. A recent study has suggested that oxidative stress leads to increased mutation frequency in a murine model of MDS, and that additional mutations lead to the progression of MDS. We have recently reported that oxidative stress marker reflects not only iron overload but also disease progression in a MDS patient (2016, Shimizu et al). So there is a possibility that the increase in oxidative stress markers may have not only caused the iron overload but also resulted in the disease progression of MDS. To clarify this point, we evaluated the association among oxidative stress marker and disease progression in eight patients with MDS. In this study, we first examined the oxidative stress marker, derivatives of reactive oxygen metabolite (dROM), ferritin in serum and Wilms Tumor 1 gene (WT1) in the peripheral blood throughout the course of treatment. Then, we analyzed the correlations of the levels of dROM, ferritin and WT1. Next, we investigated ROS expression in each fraction of WBC by flow cytometry in a healthy donor and some MDS patients. Materials and methods: Levels of serum dROM were measured by colorimetry, using samples of MDS (RA n=3, RAEB-1 n=4, RAEB-2 n=1). Ferritin in serum and WT1 in peripheral blood were also measured. Statistical analyses were performed by Pearsonfs test. The ROS expression was evaluated during the clinical course by flow cytometry. Results: Table 1 shows the relationship between dROM, ferritin and WT1 in MDS patients throughout the treatment. Case 1 to 3 patients showed disease progression with excess blasts in spite of azacitidine treatment. These patients showed a correlation tendency between dROM and ferritin, dROM and WT1. Case 1 showed close relationship between ferritin and WT1. Case 3 shows slight relation between ferritin and WT1. Case 4 to 8 patients showed no disease progression. They were treated with supportive therapy, including blood transfusion or cytokine therapy. These patients showed no relation between dROM and the elevation of ferritin and WT1. In these patients, there was no relation between ferritin and WT1 except Case 6. We also evaluated the score of WPSS, IPSS and dROM at diagnosis. dROM did not correlate with WPSS and IPSS. Figure 1 shows increased ROS expression during disease progression in a MDS patient compared with healthy control. Considering that other MDS patients with excess blasts showed the similar pattern, the present result suggested that the oxidative stress markers were produced by tumor-derived cells. Conclusions: dROM levels were slightly related with the ferritin and WT1 mRNA expression levels in MDS patients with excess blasts. We speculate that the increase in the oxidative stress markers is a cause of not only iron overload but also the disease progression of MDS. Oxidative stress markers may contribute to evaluate the progression of treatment efficacy for MDS patients. Table 1 Patientfs characteristics and correlation between serum dROM and ferritin, WT1 mRNA in MDS patients. Table 1. Patientfs characteristics and correlation between serum dROM and ferritin, WT1 mRNA in MDS patients. Figure 1 The increment of ROS expression during disease progression in a MDS patient with excess blasts. Figure 1. The increment of ROS expression during disease progression in a MDS patient with excess blasts. Disclosures Nakaseko: BMS: Honoraria, Research Funding; PFIZER: Honoraria, Research Funding; NOVARTIS: Honoraria.

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Leukocytes Count and Oxidative Stress Markers in Blood and Follicular Fluid in Infertile Women Undergo Intracytoplasmic Sperm Injection (Ic

Indian Journal Of Applied Research ◽

10.15373/2249555x/august2014/125 ◽

2011 ◽

Vol 4 (8) ◽

pp. 483-485

Author(s):

Sami R Al-Katib ◽

◽

Basima Sh AlGhazali ◽

Ban J Edan

Keyword(s):

Oxidative Stress ◽

Follicular Fluid ◽

Intracytoplasmic Sperm Injection ◽

Oxidative Stress Markers ◽

Infertile Women ◽

Stress Markers ◽

And Oxidative Stress

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Comparison of Salivary Antioxidants and Oxidative Stress Status in Gestational Diabetes Mellitus and Healthy Pregnant Women

Endocrine Metabolic & Immune Disorders - Drug Targets ◽

10.2174/1568026620666201022151059 ◽

2020 ◽

Vol 20 ◽

Author(s):

Fatemeh Ahmadi-Motamayel ◽

Shima Fathi ◽

Mohammad Taghi Goodarzi ◽

Shiva Borzouei ◽

Jalal Poorolajal ◽

...

Keyword(s):

Oxidative Stress ◽

Diabetes Mellitus ◽

Gestational Diabetes ◽

Pregnant Women ◽

Oxidative Stress Markers ◽

Blood Samples ◽

Stress Markers ◽

Total Antioxidant ◽

Total Oxidative Stress ◽

And Oxidative Stress

Background: One of the most common complications of pregnant women is gestational diabetes mellitus (GDM). Oxidative stress can play an important role in GDM. Objective: The aim of this study was to evaluate salivary antioxidants and oxidative stress markers in GDM. Method: Twenty pregnant women with GDM and 20 healthy pregnant women with normal blood glucose test participated in this study. Five mL of unstimulated saliva samples were collected. Spectrophotometric assay was carried out for sialochemical analysis. Stata software was used for data analysis. Results: The GDM group exhibited no significant difference in salivary total antioxidant capacity and malondialdehyde compared to the healthy control group. All of antioxidants markers, the uric acid, total antioxidant, peroxidase and catalase, decreased in GDM group that the difference of peroxidase and catalase was statistically significant. All of oxidative stress markers, the salivary malondyaldehid, total oxidative stress and total thiol, increased in GDM group. GDM group exhibited significantly higher salivary total oxidative stress levels. Conclusion: Catalase level was significantly lower and total oxidative stress was significantly higher. These two markers might have significant importance and might exhibit early changes compared to other factors in GDM. . Some of salivary antioxidants might have diagnostic, prognostic or therapeutic implications in GDM. Other studies with large sample size on salivary and blood samples need to be done to confirm this properties and salivary samples using instead of blood samples in GDM biomarkers changes.

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Evaluation of Salivary Antioxidants and Oxidative Stress Markers in Type 2 Diabetes Mellitus: A Retrospective Cohort Study

Endocrine Metabolic & Immune Disorders - Drug Targets ◽

10.2174/1871530319666191016103222 ◽

2020 ◽

Vol 20 (4) ◽

pp. 584-590 ◽

Cited By ~ 1

Author(s):

Shima Fathi ◽

Shiva Borzouei ◽

Mohammad Taghi Goodarzi ◽

Jalal Poorolajal ◽

Fatemeh Ahmadi-Motamayel

Keyword(s):

Oxidative Stress ◽

Diabetes Mellitus ◽

Control Group ◽

Oxidative Stress Markers ◽

Stress Markers ◽

Type 2 Dm ◽

Patients With Diabetes ◽

The Difference ◽

And Oxidative Stress

Background: Diabetes Mellitus (DM) is a progressive metabolic disorder. Objective: The aim of this study was to investigate the relationship between antioxidant and oxidative stress markers in the saliva of patients with type 2 DM and a healthy control group. Methods: In this study, 20 patients with diabetes and 20 healthy individuals were evaluated. Salivary antioxidants markers consisted of total antioxidant capacity (TAC), uric acid (UA), peroxidase and catalase. Oxidative stress markers included total oxidant status (TOS), malondealdehyde (MDA) and total thiol (SH). Sialochemical analysis was performed with spectrophotometric assay. All the statistical analyses were conducted using STATA software. Results: TAC decreased significantly in patients with diabetes. Although salivary UA and peroxidase were lower in patients with diabetes compared to the control group, the difference was not significant. Salivary catalase in patients with diabetes was significantly lower than that in the control group. MDA and TOS exhibited significantly higher levels in type 2 DM. SH levels were slightly higher in DM. Conclusions: According to the results of the present study, there were some changes in the salivary levels of some antioxidants and oxidative stress markers in patients with type 2 DM and could be measured as an indicator of serum changes..

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