Abstract P233: Microvascular Fingerprint Of Multi-Organ Imaging - New Insights In Preeclampsia Research

Hypertension ◽  
2021 ◽  
Vol 78 (Suppl_1) ◽  
Author(s):  
Kristin Kraeker ◽  
Hanna Napieczynska ◽  
Sarah Kedziora ◽  
Nadine Haase ◽  
Dominik N. Müller ◽  
...  
Keyword(s):  
Structural Changes ◽  
Three Dimensional ◽  
Ct Scanning ◽  
Vessel Diameter ◽  
Simultaneous Increase ◽  
Micro Ct ◽  
Multiple Organs ◽  
Increased Risk ◽  
Inflammatory Processes ◽  
Vascular Physiology

To establish the first protocol for high-resolution three-dimensional imaging of multiple organs in rodents, we performed microvasculature casting with Microfil silicone rubber substance in non-living rats and combined the method with advanced image analysis protocols after micro-CT scanning. This newly established method allows changes in individual organs to be visualized and examined in extraordinary detail as part of a multi-organ approach. Previously published protocols only address visualization of single organs or are insufficiently resolved with respect to vessel diameter analysis. With optimized staining protocols, samples can additionally be examined for possible structural changes such as inflammatory processes or fibrotic formation. Preeclampsia is a condition during pregnancy that leads to up to a 4-fold increased risk of maternal cardiovascular disease later in life, although symptoms usually resolve after delivery. In former preeclamptic rats, a reduction in microvascular density in the heart and brain with a simultaneous increase in retinal vessels has been described, even weeks after the disappearance of major features such as hypertension and albuminuria. The technique described will enhance studies of the vasculature in many other animal models and provide striking insights into vascular physiology necessary to drive translation.

scholarly journals A Microcomputed Tomographic Evaluation of Root Canal Morphology of Maxillary Second Premolars in a Pakistani Cohort

2021 ◽  
Vol 11 (11) ◽  
pp. 5086
Author(s):  
Mazen F. Alkahtany ◽  
Saqib Ali ◽  
Abdul Khabeer ◽  
Shafqat A. Shah ◽  
Khalid H. Almadi ◽  
...  
Keyword(s):  
Root Canal ◽  
Three Dimensional ◽  
Ct Scanning ◽  
Micro Ct ◽  
Single Root ◽  
Root Canal Morphology ◽  
Spss Software ◽  
Variable Morphology ◽  
Canal Morphology

This study aimed to investigate variations in the root canal morphology of maxillary second premolar (MSP) teeth using microcomputed tomography (micro-CT). Sixty (N = 60) human extracted MSPs were collected and prepared for micro-CT scanning. The duration for scanning a single sample ranged between 30 and 40 min and a three-dimensional (3-D) image was obtained for all the MSPs. The images were evaluated by a single observer who recorded the canal morphology type, number of roots, canal orifices, apical foramina(s), apical delta(s), and accessory canals. The root canal configuration was categorized in agreement with Vertucci’s classification, and any configuration not in agreement with Vertucci’s classification was reported as an “additional canal configuration”. Descriptive statistics (such as mean percentages) were calculated using SPSS software. The most common types agreeing with Vertucci’s classification (in order of highest to lowest incidence) were types I, III, V, VII, II, and VI. The teeth also exhibited four additional configurations that were different from Vertucci’s classification: types 2-3, 1-2-3, 2-1-2-1, and 1-2-1-3. A single root was found in 96.7% and the majority of the samples demonstrated two canals (73.3%). Further, 80% of the teeth showed one canal orifice. The number of apical foramina’s in the teeth was variable, with 56.7% having solitary apical foramen. The accessory canal was found in 33.3%, and apical delta was found in only 20% of the samples. Variable morphology of the MSPs was detected in our study. The canal configuration most prevalent was type 1; however, the results also revealed some additional canal types.

scholarly journals Micro-CT data of early physiological cancellous bone formation in the lumbar spine of female C57BL/6 mice

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Michael Zenzes ◽  
Paul Zaslansky
Keyword(s):  
Cancellous Bone ◽  
Structural Changes ◽  
Bone Growth ◽  
Post Partum ◽  
Three Dimensional ◽  
Lumbar Vertebra ◽  
Tissue Growth ◽  
Micro Ct ◽  
Mineral Density ◽  
Ct Data

AbstractMicro-CT provides critical data for musculoskeletal research, yielding three-dimensional datasets containing distributions of mineral density. Using high-resolution scans, we quantified changes in the fine architecture of bone in the spine of young mice. This data is made available as a reference to physiological cancellous bone growth. The scans (n = 19) depict the extensive structural changes typical for female C57BL/6 mice pups, aged 1-, 3-, 7-, 10- and 14-days post-partum, as they attain the mature geometry. We reveal the micro-morphology down to individual trabeculae in the spine that follow phases of mineral-tissue rearrangement in the growing lumbar vertebra on a micrometer length scale. Phantom data is provided to facilitate mineral density calibration. Conventional histomorphometry matched with our micro-CT data on selected samples confirms the validity and accuracy of our 3D scans. The data may thus serve as a reference for modeling normal bone growth and can be used to benchmark other experiments assessing the effects of biomaterials, tissue growth, healing, and regeneration.

StyroStone: A protocol for scanning and extracting three-dimensional meshes of stone artefacts using Micro-CT scanners v1

2021 ◽  
Author(s):  
Dominik Göldner ◽  
Fotios Alexandros Karakostis ◽  
Armando Falcucci
Keyword(s):  
Three Dimensional ◽  
Computation Time ◽  
Ct Scanning ◽  
3D Models ◽  
Stone Tools ◽  
Surface Model ◽  
Micro Ct ◽  
Ct Scanner ◽  
Micro Computed Tomography ◽  
Ct Scanners

This protocol presents the first detailed step-by-step pipeline for the 3D scanning and post processing of large batches of lithic artefacts using a micro-computed tomography (micro-CT) scanner (i.e., a Phoenix v-tome-x S model by General Electronics MCC, Boston MA) and an Artec Space Spider scanner (Artec Inc., Luxembourg). This protocol was used to scan and analyze ca. 700 lithic artefacts from the Protoaurignacian layers at Fumane Cave in north-eastern Italy (Falcucci et al., in preparation). For this study several costly scanners and proprietary software packages were employed. Although it is not easy to find a low-budget alternative for the scanners, it is possible to use free and open-source software programs, such as 3D-Slicer (https://www.slicer.org/) or MorphoDig (https://morphomuseum.com/morphodig), to process CT data as well as MeshLab (Cignoni et al. 2008) to interact with the 3D models in general. However, if alternative software is used, the steps and their order described in this protocol might diverge significantly. A cost-effective alternative to create 3D models is digital photogrammetry using commercial cameras and freely available software like Meshroom (https://alicevision.org). Although photogrammetry is an affordable technique to create accurate 3D models of objects, this method might not be useful when scanning large batches of artefacts, as it will require a lot of computation time and processing capacity. Likewise, it could be difficult to generate accurate 3D models of very small and/or detailed tool shapes using 3D surface scanners because stone tools are often much smaller than the recommended minimum field of view. Similarly, the resolution of conventional medical CT scanners might not be sufficient to capture minor details of stone tools, such as the outline or dorsal scars. Thus, high-resolution micro-CT technology is the only reliable way to accurately capture the overall morphology of small stone tools. This protocol aims at providing the first detailed procedure dedicated to the scanning of small lithic implements for further three-dimensional analysis. Note that some of the steps must be repeated at different working stages throughout this protocol. In cases where a task must be done in the exact same way as described in a previous step, a reference to that step is provided. When slight changes were made, the step was modified and reported entirely. This protocol contains a few red and green colours (e.g., arrows or within-program colours) which might be perceived differently by people with dyschromatopsia. However, the display of these colours has been kept to a minimum. We recommend the reader to go over the entire protocol carefully, even if only some specific parts are required. A few points are in fact interdependent, and some of them must be applied simultaneously. Content: Part 1 – Styrofoam preparation Part 2 – Micro-CT scanning Part 3 – 3D model extraction of CT scanned stone artifacts using Avizo Part 4 – Cropping extracted surface model to separate Face A and B in Artec Studio Part 5 – Cropping Face A to separate the lines in Artec Studio Part 6 – Cropping each stone artefact from the lines in Artec Studio Part 7 – Virtually control measurements in MeshLab Part 8 – Artec scanning of larger artifacts Part 9 – Export meshes as non-binary ply models for successive analysis in geomorph Three-dimensional example (in ply format) of the effectivity of the StyroStone Protocol: You can download an example of one Styrofoam line in 3D obtained using our protocol to appreciate the result that can be achieved. We have selected a line where objects are characterized by different metric and morphological attributes. Notice the retouching well visible in the last five smaller artifacts (counting from the left when artifact are oriented with the dorsal face in front of the observer and the butt down), as well as the platforms and bulbs of all artifacts. For more information and examples, feel free to contact us!

scholarly journals An exceptionally preserved euarthropod with unique feather-like appendages from the Chengjiang biota

2021 ◽  
Author(s):  
Dayou Zhai ◽  
Mark Williams ◽  
David J. Siveter ◽  
Derek J. Siveter ◽  
Thomas H.P. Harvey ◽  
...  
Keyword(s):  
Soft Part ◽  
Three Dimensional ◽  
Ct Scanning ◽  
Early Cambrian ◽  
Micro Ct ◽  
Burgess Shale ◽  
Terminal Seta ◽  
Stem Group ◽  
Chengjiang Biota

AbstractMicro-CT scanning of the Cambrian euarthropod Chuandianella ovata reveals unprecedented three-dimensional soft-part details. It has an elongate uniramous antennule and a short uniramous second appendage, followed by ten homonomous biramous appendages, each comprising a short paddle-shaped exopod and a unique feather-like endopod with at least 27 podomeres each of which bears a long blade-like endite with a short terminal seta. Based on its carapace and previously known soft-part anatomy C. ovata was compared with the Burgess Shale mandibulate euarthropod Waptia. However, Waptia has recently been shown to bear specialized head appendages that are interpreted as a mandible and maxillula, posterior to which are four appendages each with five-segmented endopods. In contrast, we interpret Chuandianella as an ‘upper’ stem-group euarthropod that possessed neither a differentiated mandible nor a maxillula. Chuandianella further demonstrates that early Cambrian ‘upper’ stem-group euarthropods were experimenting with a range of different limb arrangements and morphologies.

scholarly journals Marginal and Internal Adaptation Evaluation of Dental Composites Using Micro-CT

2019 ◽  
Vol 2019 ◽  
pp. 1-6
Author(s):  
Adrian Tudor Stan ◽  
Laura Cîrligeriu ◽  
Laura Idorași ◽  
Meda Lavinia Negruțiu ◽  
Cosmin Sinescu ◽  
...  
Keyword(s):  
Composite Resin ◽  
Resin Composite ◽  
Three Dimensional ◽  
Ct Scanning ◽  
Dental Composite ◽  
Micro Ct ◽  
Gap Formation ◽  
Micro Computed Tomography ◽  
Vertical Slot ◽  
Internal Adaptation

The aim of the study was to determine the marginal and internal adaptation after curing of different composite resin restorations, using a nondestructive X-ray micro-computed tomography (micro-CT). Forty previously extracted human molars, kept in 10% formalin to preserve the bonding capabilities of the tooth structures, were divided into four groups based on the composite system used and different light-curing times. Class II (vertical slot) cavities were prepared on one proximal side and restored with dental composite using a Tofflemire matrix, with a bulk-fill composite resin (Venus Bulk Fill, Heraeus Kulzer) and a universal posterior composite (G-ænial Posterior, GC). A curing lamp (Kerr Demi Ultra) was used with different curing times. Micro-CT scanning was performed by using Nikon XTH 225ST to reveal any defects in adaptation or gaps at the tooth restoration interface. The 3D images of the adaptation around the restorations were reconstructed using VG Studio Max 2.2 and myVGL 2.2.6 64-bit software. All samples from the G-ænial group showed marginal and internal gaps, with G-ænial Posterior having higher polymerization shrinkage and marginal gap values. In the Venus Bulk Fill group, there were fewer restorations with internal and external gap formation. Micro-CT is a three-dimensional imaging technique that can nondestructively detect adaptation around the resin composite restorations at every level of the sample.

scholarly journals Nondestructive cryomicro-CT imaging enables structural and molecular analysis of human lung tissue

2017 ◽  
Vol 122 (1) ◽  
pp. 161-169 ◽  
Author(s):  
Dragoş M. Vasilescu ◽  
André B. Phillion ◽  
Naoya Tanabe ◽  
Daisuke Kinose ◽  
David F. Paige ◽  
...  
Keyword(s):  
Molecular Analysis ◽  
Structural Changes ◽  
Human Lung ◽  
Three Dimensional ◽  
Ct Imaging ◽  
Matrix Composition ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
Human Lung Tissue ◽  
Lung Structure

Micro-computed tomography (CT) enables three-dimensional (3D) imaging of complex soft tissue structures, but current protocols used to achieve this goal preclude cellular and molecular phenotyping of the tissue. Here we describe a radiolucent cryostage that permits micro-CT imaging of unfixed frozen human lung samples at an isotropic voxel size of (11 µm)3 under conditions where the sample is maintained frozen at −30°C during imaging. The cryostage was tested for thermal stability to maintain samples frozen up to 8 h. This report describes the methods used to choose the materials required for cryostage construction and demonstrates that whole genome mRNA integrity and expression are not compromised by exposure to micro-CT radiation and that the tissue can be used for immunohistochemistry. The new cryostage provides a novel method enabling integration of 3D tissue structure with cellular and molecular analysis to facilitate the identification of molecular determinants of disease. NEW & NOTEWORTHY The described micro-CT cryostage provides a novel way to study the three-dimensional lung structure preserved without the effects of fixatives while enabling subsequent studies of the cellular matrix composition and gene expression. This approach will, for the first time, enable researchers to study structural changes of lung tissues that occur with disease and correlate them with changes in gene or protein signatures.

scholarly journals MIP and CT Scanning Characterization of Pore Structure of Tight Volcanic Rocks

2021 ◽  
Author(s):  
Yunliang Yu ◽  
Haibo Xu ◽  
Ye Bai ◽  
Huijing Zhang ◽  
Wenqing Niu ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Pore Structure ◽  
Oil And Gas ◽  
Volcanic Rocks ◽  
Three Dimensional ◽  
Ct Scanning ◽  
Micro Ct ◽  
Gas Reservoirs ◽  
Rock Samples ◽  
Unconventional Oil And Gas

Volcanic rocks are unconventional oil and gas reservoirs, and the pore structure of the rock is relatively complex. In this study, we selected samples from CS105 well and DS9 well from Yingcheng Formation volcanic reservoirs in the southern Songliao Basin to study the pore structure. First, we performed experiments on two rock samples using computed tomography (CT), and then measured the porosity and density of the two rock samples using mercury intrusion porosimetry (MIP). According to the data obtained by micron computed tomography (Micro-CT), Avizo 2019.1 software was used to perform three-dimensional (3D) reconstruction on the micron level of the two wellhead samples. Analyze the microscopic pore structure of the rock sample and compare it with the porosity of the sample obtained by the mercury injection method. Compared with MIP, Micro-CT more accurately characterizes the pore structure and porosity of volcanic rocks.

scholarly journals Finding Appropriate Nickel-Titanium Instruments for Lingual Canals in Mandibular First Premolars with Two Canals: A Micro-CT Study

2021 ◽  
Author(s):  
Yunqin Wang ◽  
Yaqian Lu ◽  
Yongchun Gu ◽  
Yuhua Xiong ◽  
Yixin Cai ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Ct Scanning ◽  
Nickel Titanium ◽  
3D Analysis ◽  
Micro Ct ◽  
Apical Transportation ◽  
Significant Difference ◽  
Dimensional Parameters ◽  
Mandibular First Premolar ◽  
Better Than

Abstract Background: To find appropriate Nickel-Titanium instruments for lingual canals in mandibular first premolars with two canals.Methods: A total of 42 extracted mandibular first premolars with two canals were selected after micro-CT scanning. The teeth were matched and divided into three groups, and three types of Nickel-Titanium instruments (M3, HyFlex CM and XP-endo Shaper) were used to prepare the lingual canals of the sample teeth. After instrumentation, micro-CT scanning was used again to reconstruct the three-dimensional (3D) teeth model, and then it was geometrically aligned with the original model before instrumentation. Two-dimensional (2D) and three-dimensional parameters of teeth models were measured to evaluate the ability of cleaning and maintaining the original canal shape by the three different instruments. Results: Compared with HyFlex CM and XP-endo Shaper, the apical transportation of M3 was significantly greater within the apical 3 mm (P < 0.05), while there was no significant difference between HyFlex CM and XP-endo Shaper (P > 0.05). There was no significant difference in centering ability among three types of instruments (P > 0.05). In general, HCM group and XPS group performed better than M3 group in the 2D and 3D analysis (P < 0.05). Conclusions: Preparation of the lingual canal in mandibular first premolar was more challenging than that of the main buccal canal. All the three nickel-titanium instruments are safe for canal preparation, and generally, the HyFlex CM and XP-endo Shaper performed better than the M3.

Application of stereological analysis of cell volume to isolated myocytes in culture with and without adrenergic innervation

1990 ◽  
Vol 48 (3) ◽  
pp. 416-417
Author(s):  
Jane K. Rosenthal ◽  
Dianne L. Atkins ◽  
William J. Marvin ◽  
Penny A. Krumm
Keyword(s):  
Cardiac Myocytes ◽  
Structural Changes ◽  
Three Dimensional ◽  
Adrenergic Innervation ◽  
Culture Cell ◽  
Serial Sections ◽  
Newborn Rats ◽  
Primary Culture Cell ◽  
Biological Studies ◽  
Cell Volumes

To comprehend structural changes in cardiac myocytes accompanying adrenergic innervation, it is essential that a three dimensional analysis be performed. To date, biological studies which utilize stereological methods have been limited to cells in tissue and in organs. Our laboratory has utilized current stereological techniques for measuring absolute volumes of individual myocytes in primary culture. Cell volumes are calculated for two distinct groups of cells at 96 hours in culture: isolated myocytes and myocytes innervated with adrenergic neurons (Figure 1).Cardiac myocytes are cultured from the ventricular apices of newborn rats. Cells are plated directly onto tissue culture dishes with or without preplated explants from the paravertebral thoracolumbar sympathetic chain. On day four cultures are photographed and marked for one-to-one cell location. Following conventional fixation and embeddment in eponate-12, the cells are relocated and mounted for microtomy. The cells are completely sectioned at 120nm in their parallel orientation to the surface of the dish (Figure 2). Serial sections are collected on formvar coated slotted grids and are recorded in sequence.

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