scholarly journals Morphological, ecological and molecular characterization of Pyropia vietnamensis (Bangiales, Rhodophyta) from the Konkan region, India

Phytotaxa ◽  
2015 ◽  
Vol 224 (1) ◽  
pp. 45 ◽  
Author(s):  
Monica Gajanan Kavale ◽  
Mudassar Anisoddin Kazi ◽  
Nived Sreenadhan ◽  
Veerendra Veer Singh
Keyword(s):  
Plant Morphology ◽  
Close Relation ◽  
Morphological Characters ◽  
Molecular Characteristics ◽  
Ecological Parameter ◽  
Indian Waters ◽  
Taxonomic Implications ◽  
Ecological Parameters ◽  
Cox 1

Pyropia vietnamensis is one of the most luxuriously growing seaweed in Konkan coast, India. In the present study we attempted to explore the morphology, ecology and molecular characteristics exhibited by P. vietnamensis and its taxonomic implications. We described the effects of ecological parameters on the variability of morphological characters. The water motion was found to be one of the most important ecological parameter that governs the plant morphology. The plant was found to grow on entire intertidal region and this may be one of the possible reasons for high morphological variation among the taxa in this region. The molecular analysis utilizing cox 1 gene sequences clearly revealed the close relation of Indian and Brazilian specimens with only 0-1 bp intraspecific variation. The present study provides a beginning to a clearly required detailed study of the morphology, ecology and genetic diversity showed by Pyropia species from Indian waters.

scholarly journals Morphological, Cytological and Embryological Characterization of F1 A. cepa × A. roylei Hybrids

2015 ◽  
Vol 57 (2) ◽  
pp. 98-105 ◽  
Author(s):  
Alicja Chuda ◽  
Karolina Kłosowska ◽  
Adela Adamus
Keyword(s):  
Female Gametophyte ◽  
Parental Species ◽  
Pollen Viability ◽  
Plant Morphology ◽  
Morphological Characters ◽  
Tetrad Stage ◽  
Pollen Mother Cells ◽  
Developmental Disturbances ◽  
Mother Cells

Abstract In the previous study we obtained a population of interspecific F1 A. cepa × A. roylei hybrids. In this study, in comparison to the parental species: A. cepa and A. roylei, the F1 hybrids were evaluated in terms of plant morphology, pollen viability, microsporogenesis and female gametophyte. Most of the morphological characters of the F1 hybrids were intermediate as compared to those of both parental accessions. In pollen mother cells (PMCs) of the F1 hybrids abnormalities were observed in meiosis as well as at the tetrad stage. Pollen viability of F1 A. cepa × A. roylei hybrids was reduced to 30.1%. In the F1 hybrids, 45.8% of the analyzed ovules showed developmental disturbances, whereas in 26.7% of the ovules necrotic processes were observed.

Morphological and molecular characterization of underutilized medicinal wild ginger (Zingiber barbatum Wall.) from Myanmar

2011 ◽  
Vol 9 (4) ◽  
pp. 531-542 ◽  
Author(s):  
Noladhi Wicaksana ◽  
Syed Abdullah Gilani ◽  
Dawood Ahmad ◽  
Akira Kikuchi ◽  
Kazuo N. Watanabe
Keyword(s):  
Genetic Diversity ◽  
Growth Habit ◽  
Crop Improvement ◽  
Morphological Characters ◽  
Molecular Characteristics ◽  
Medicinal Species ◽  
Primer Sets ◽  
High Degree ◽  
Morphological And Molecular Characterization

Zingiber barbatum Wall. (family Zingiberaceae), is an underutilized medicinal plant and commonly known as ‘Meik tha-lin’ in Myanmar where it is used in the indigenous system of medicine. In the present study, 19 accessions of Z. barbatum from five provinces in Myanmar have been utilized to characterize and assess genetic diversity. Twenty-nine morphological characters were noted, including growth habit, leaf, pseudo-stem and rhizome characters. Fifteen primer sets of P450-based analogue (PBA) markers were used to reveal molecular characteristics. Of the 29 morphological characters, 22 showed a high degree of variation within wild ginger accessions, whereas 20 of these characters contributed significantly to morphological variation. Eleven amplified primer sets gave a total of 175 bands and exhibited 92.15% polymorphism across Z. barbatum accessions. Based on morphological characters and PBA markers, 19 accessions can be divided into two morphotype groups with comparatively higher genetic diversity. This information can be applied in future crop improvement, proper conservation and better use of this underutilized medicinal species.

First Molecular Characterization of Aspiculuris tetraptera (Nematoda: Heteroxynematidae) from Mus musculus (Rodentia: Muridae) in India

2015 ◽  
Vol 60 (3) ◽  
Author(s):  
Urvashi Goswami ◽  
Anshu Chaudhary ◽  
Chandni Verma ◽  
Hridaya Shanker Singh
Keyword(s):  
Molecular Characterization ◽  
Mus Musculus ◽  
18S Rrna ◽  
Molecular Study ◽  
Morphological Characters ◽  
Present Communication ◽  
Dna Analyses ◽  
Aspiculuris Tetraptera ◽  
Cox 1

AbstractMus musculus (Rodentia: Muridae) were infected by a nematode belonging to the order Oxyurida. According to external morphological characters it was found to be Aspiculuris tetraptera Schulz, 1924. Present communication deals with molecular characterization of A. tetraptera infecting intestine of Mus musculus in Meerut (U.P.), India. A partial sequence of 18S ribosomal and cytochrome c oxidase subunit 1 (Cox 1) mitochondrial DNA analyses were performed. Sequence corresponding to 18S rRNA and mt Cox 1 gene was identical to sequences reported for A. tetraptera on GenBank. These results confirm the taxonomic validation of A. tetraptera parasitizing Mus musculus. This is the first report of molecular study of A. tetraptera in India.

A Characterization of Some Geometries of Chains

1974 ◽  
Vol 26 (02) ◽  
pp. 257-272 ◽  
Author(s):  
Yi Chen
Keyword(s):  
Projective Plane ◽  
Minkowski Plane ◽  
Close Relation ◽  
Laguerre Plane ◽  
Geometric Structures ◽  
Möbius Plane

The geometries considered here are the Möbius plane M() (W. Benz [1]), the Laguerre plane L() (W. Benz and H. Mäurer [7]) and the Minkowski plane A() (W. Benz [5], G. Kaerlein [18]) over a field . All of them are geometries of an algebra with identity over a field. The characterization of the projective plane over a field by the proposition of Pappus first gave a close relation between algebraic and geometric structures. B. L. v. d. Waedern and L. J. Smid [28] presented a further example by characterizing the Möbius and Laguerre plane with incidence axioms and the "complete" proposition of Miquel.

scholarly journals MORPHOLOGICAL AND CHROMOSOMAL CHARACTERIZATION OF ORCHID Peristylus goodyeroides Lindl. FROM CURUG SETAWING, KULONPROGO

2021 ◽  
Vol 12 (2) ◽  
pp. 110
Author(s):  
Febri Yuda Kurniawan ◽  
Anindita Della Rosa Riyadi
Keyword(s):  
Leaf Shape ◽  
Ecological Factors ◽  
Morphological Characters ◽  
Morphological Characterization ◽  
Observation Data ◽  
Chromosome Configuration ◽  
Telocentric Chromosomes ◽  
Leaf Arrangement ◽  
Karyotype Formula

Peristylus goodyeroides is a terestrial orchid that scattered around Southeast Asia. Morphological characters of P. goodyeroides can vary, depending on the ecological factors and habitat. Cytological characters in the form of chromosome configurations can be used as a taxonomic tool for the process of identifying and understanding variations in taxa. The purpose of this study was to determine the morphological characters and chromosome configuration of the P. goodyeroides from Curug Setawing, Kulonprogo. The method used was morphological characterization and plant chromosome preparation by squash method with the main steps of fixation, maceration, staining and observation. Data were analyzed with the help of Image raster 3, Corel Draw X7, and Microsoft Excel 2013. P. goodyeroides from Curug Setawing has the morphological characters of root tubers, cylindrical stems, ovate leaf shape, convolutive leaf arrangement and creamy white flowers. The orchid has a number of chromosomes 2n = 10 with a karyotype formula of 2n = 2x = 8m + 2t. Metacentric chromosomes are found on chromosomes 1-8 and telocentric chromosomes 9-10. The absolute arm length of the chromosomes has a range of 2.03-3.44 μm, the relative arm length of the chromosomes is 2.21-3.32 μm, the length of the p arm is 1.13-1.58 μm and the q arm is 1.23-2.12 μm.

scholarly journals Phenotypic and Molecular Characteristics of the MDR Efflux Pump Gene-Carrying Stenotrophomonas maltophilia Strains Isolated in Warsaw, Poland

Biology ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 105
Author(s):  
Olga M. Zając ◽  
Stefan Tyski ◽  
Agnieszka E. Laudy
Keyword(s):  
Molecular Typing ◽  
Stenotrophomonas Maltophilia ◽  
Efflux Pump ◽  
Pulsed Field Gel Electrophoresis ◽  
Molecular Characteristics ◽  
The World ◽  
Genes Encoding ◽  
Resistance Nodulation Division ◽  
Sequence Types

An increase of nosocomial infections caused by Stenotrophomonas maltophilia strains has recently been observed all over the world. The isolation of these bacteria from the blood is of particular concern. In this study we performed the phenotypic and genotypic characterization of 94 S. maltophilia isolates, including isolates from patients hospitalized in a tertiary Warsaw hospital (n = 79) and from outpatients (n = 15). All isolates were found to be susceptible to trimethoprim-sulfamethoxazole and minocycline, while 44/94 isolates demonstrated a reduction in susceptibility to levofloxacin. A large genetic variation was observed among the isolates tested by pulsed-field gel electrophoresis. A clonal relationship with 100% similarity was observed between isolates within two sub-pulsotypes: the first included nine bloodstream isolates and the second involved six. Multilocus sequence typing showed two new sequence types (ST498 and ST499) deposited in public databases for molecular typing. Moreover, the presence of genes encoding ten different efflux pumps from the resistance-nodulation-division family and the ATP-binding cassette family was shown in the majority of the 94 isolates. The obtained knowledge about the prevalence of efflux pump genes in clinical S. maltophilia strains makes it possible to predict the scale of the risk of resistance emergence in strains as a result of gene overexpression.

scholarly journals Comparative cytogenetics of two species of Dermanura (Chiroptera, Phyllostomidae) in Midwestern Brazil

2021 ◽  
Vol 15 (2) ◽  
pp. 89-99
Author(s):  
Ricardo Firmino de Sousa ◽  
Paulo Cesar Venere ◽  
Karina de Cassia Faria
Keyword(s):  
Diploid Number ◽  
Constitutive Heterochromatin ◽  
Sex Chromosome ◽  
Type System ◽  
Pericentromeric Region ◽  
Morphological Characters ◽  
Comparative Cytogenetics ◽  
Taxonomic Implications ◽  
Sex Chromosome System ◽  
First Time

Dermanura Gervais, 1856 is represented by small frugivorous bats of the Stenodermatinae subfamily. The taxonomy of this group presents controversies and has been subject to changes, especially since the morphological characters evaluated have left gaps that are difficult to fill regarding good species characterization. Previous studies performed in Dermanura cinerea Gervais, 1856 found that the karyotype of this species has a diploid number of chromosomes equal to 30 and 56 autosomal arms. The objective of the present study was to describe, for the first time, the karyotypes of the species Dermanura anderseni (Osgood, 1916) and Dermanura gnoma (Handley, 1987) based on classical cytogenetic markers. For both species, the diploid number found was 2n = 30 and NFa = 56. Two pairs of chromosomes showed markings of the nucleolus organizing regions (AgNORs) in the species D. anderseni and only one pair in D. gnoma, differing from what has already been described for D. cinerea. The two species analyzed here also showed differences in the sex chromosome system, with D. gnoma showing a neo-XY type system while in D. anderseni the classic XY sexual system was observed. In both species, visualization of the constitutive heterochromatin occurred in the pericentromeric region of all chromosomes, as well as in the short arms of the subtelocentric chromosomes. The present work represents an important expansion of karyotypic information for the subfamily Stenodermatinae, bringing chromosomal features that are possible to use in the taxonomic implications of the group.

scholarly journals Morphological and cultural characterization of Phyllosticta zingiberi (Ramkr.) causing leaf spot disease of ginger

2017 ◽  
Vol 9 (3) ◽  
pp. 1662-1665
Author(s):  
Barun Rai ◽  
Sekhar Bandyopadhyay ◽  
Avisak Thapa ◽  
Adeetya Rai ◽  
Deewakar Baral
Keyword(s):  
Carbon Source ◽  
Leaf Spot ◽  
Microscopic Observation ◽  
Zingiber Officinale ◽  
Maximum Growth ◽  
Morphological Characters ◽  
Leaf Spot Disease ◽  
Malt Extract ◽  
Brown Colour

Isolation of ginger (Zingiber officinale) leaf spot pathogen form the UBKV farm field was done in the laboratory. The isolated pathogen was identified as Phyllosticta zingiberi on the basis of morphological characters as documented in taxonomic keys. The microscopic observation revealed that the pycnidia were globose to subglobose with dark brown colour measuring 124.16 μm × 2.35 μm in average. The pycnidio spores were hyaline, oval to bullet shaped, monoguttulate measuring 4.02 μm × 2.35 μm in average. Among the different media tested for growth highest growth was recorded in Oat meal agar (26.44 cm2) followed by malt extract agar (24.04 cm2) which was statistically at par. The temperature of 25˚C favoured maximum growth (24.20 cm2). However, higher sporulation was observed in 30˚C. Among the different carbon source tested, mannitol supported the highest growth of the pathogen (27.67 cm2).

Morphological and molecular characterization of adult worms of Leucochloridium paradoxum Carus, 1835 and L. perturbatum Pojmańska, 1969 (Digenea: Leucochloridiidae) from the great tit, Parus major L., 1758 and similarity with the sporocyst stages

2013 ◽  
Vol 88 (4) ◽  
pp. 506-510 ◽  
Author(s):  
I. Rząd ◽  
P. Hofsoe ◽  
R. Panicz ◽  
J.K. Nowakowski
Keyword(s):  
Parus Major ◽  
Morphological Characters ◽  
Great Tit ◽  
Molecular Techniques ◽  
Morphological Identification ◽  
The Baltic Sea ◽  
The Baltic ◽  
Morphological And Molecular Characterization ◽  
Sea Coast

AbstractUnlike the sporocyst stages, adult leucochloridiid digeneans are difficult to differentiate. Sporocyst broodsacs can be identified on the basis of their colour and banding pattern, but in the absence of broodsacs and when experimental infection cannot be performed, tentative morphological identification needs to be verified, and molecular techniques offer a tool to do this. In this study, adult leucochloridiid digeneans were collected from the great tit (Parus major) found dead at three localities at or near the Baltic Sea coast (Hel, Bukowo-Kopań and Szczecin) in northern Poland. On the basis of differences in their morphological characters, Hel specimens were tentatively assigned to Leucochloridium perturbatum, Bukowo-Kopań and Szczecin specimens being identified tentatively as L. paradoxum. Subsequent ribosomal DNA sequence analysis confirmed the identification of these leucochloridiid flukes. Nucleotide sequences discriminating between the two species were identical to those used by earlier authors as characteristic of two distinctly different sporocyst broodsacs representing L. perturbatum and L. paradoxum.

scholarly journals Molecular characterization of isolated infectious bronchitis viruses from affected vaccinated broiler flocks in Syria

2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Tamara Al-Jallad ◽  
Morshed Kassouha ◽  
Mohamad Salhab ◽  
Anouar Alomar ◽  
Mouhamad AL-Masalma ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Hypervariable Region ◽  
Effective Vaccine ◽  
Molecular Characteristics ◽  
Poultry Industry ◽  
S1 Gene ◽  
Infectious Bronchitis ◽  
Epidemiological Monitoring ◽  
Immunological Protection

Abstract Background Avian Infectious Bronchitis Virus (IBV) is a highly contagious disease that imposes a huge economic burden on the global poultry industry. IBV contains numerous serotypes and variants with incomplete tenuous cross immunological protection. The failure of currently used vaccines to protect against diverse, circulating IBV strains that are specific to a given region poses a major problem for the poultry industry. Thus, there is an urgent need to conduct studies aimed at genotyping field IB viruses. In this study, we have determined the molecular characteristics of circulating IBV by sequencing the S1 gene of viral isolates from affected previously vaccinated broiler flocks suffering from the disease. Results Ten isolates propagated in embryonated eggs showed an ability to induce typical IBV lesions after three successive viral passages. We performed a nested RT–PCR assay that targeted the hypervariable region 3 (HVR-3) of the S1 gene, and identified the isolates as IBV through sequence analysis. The IBV isolates showed sequence similarity between the Syrian isolates that vary from 96.20 to 100%, and those being closer to the Variant-2 strain IS/1494/06 (EU780077.2) with 97.5 to 99.4% similarities. However, less nucleotide identity was found with sequences belonging to the used vaccine strains such as H120, Mass5, and 4/91. Conclusions This study showed the presence of the Variant-2 strain circulating in Syrian broiler flocks showing signs of IBV disease. Currently, there is no commercial effective vaccine which protects birds against the Variant-2 strain. Continuous monitoring procedures should be taken to control and limit the spread of the IBV Variant-2 strain. This research emphasizes both the importance of epidemiological monitoring in intensive poultry farming for novel pathogens and the use of local isolates as future vaccine targets.

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