First record of Epistylis plicatilis (Ciliophora: Peritrichia) attached to Pomacea canaliculata (Mollusca: Gastropoda) in Southern Brazil

Zootaxa ◽  
2007 ◽  
Vol 1454 (1) ◽  
pp. 49-57 ◽  
Author(s):  
LAURA R.P. UTZ
Keyword(s):  
Staining Technique ◽  
First Record ◽  
Morphological Characterization ◽  
Contractile Vacuole ◽  
Pomacea Canaliculata ◽  
Southern Brazil ◽  
Freshwater Environments

A more complete morphological characterization of Epistylis plicatilis Ehrenberg, 1831, a colonial peritrich epibiotic on the gastropod Pomacea canaliculata (Lamarck) in two freshwater environments in Southern Brazil was provided in the present study. The colonial sessile E. plicatilis possessed elongate zooids that range in size in vivo from 105.0 to187.5 μ m in length and from 25.0 to 52.5 μ m in width. The cell had a single contractile vacuole located near the peristome. A “C-shaped” macronucleus lay in the middle of the cell. Colonies of E. plicatilis had up to 100 zooids, but the majority possessed 10–20 zooids that were similar in size. The oral ciliature revealed by the protargol staining technique consisted of an outer haplokinety and an inner polykinety that performs approximately 1 ½ turns around the perimeter of the peristome before entering the infundibulum. Three infundibular polykineties each consisting of three rows of kinetosomes were identified. This is the first record of E. plicatilis in Brazil.

scholarly journals A Novel Genotype and First Record of Trypanosoma lainsoni in Argentina

Pathogens ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 731
Author(s):  
Anahí G. Díaz ◽  
Paula G. Ragone ◽  
Fanny Rusman ◽  
Noelia Floridia-Yapur ◽  
Rubén M. Barquez ◽  
...  
Keyword(s):  
Host Range ◽  
First Record ◽  
Morphological Characterization ◽  
Pairwise Identity ◽  
Wild Mammals ◽  
Transmission Electron ◽  
Chaco Region ◽  
Veterinary Importance ◽  
First Time

Trypanosomes are a group of parasitic flagellates with medical and veterinary importance. Despite many species having been described in this genus, little is known about many of them. Here, we report a genetic and morphological characterization of trypanosomatids isolated from wild mammals from the Argentine Chaco region. Parasites were morphologically and ultrastructurally characterized by light microscopy and transmission electron microscopy. Additionally, 18s rRNA and gGAPDH genes were sequenced and analyzed using maximum likelihood and Bayesian inference. Morphological characterization showed clear characteristics associated with the Trypanosoma genus. The genetic characterization demonstrates that the studied isolates have identical sequences and a pairwise identity of 99% with Trypanosoma lainsoni, which belongs to the clade of lizards and snakes/rodents and marsupials. To date, this species had only been found in the Amazon region. Our finding represents the second report of T. lainsoni and the first record for the Chaco region. Furthermore, we ultrastructurally described for the first time the species. Finally, the host range of T. lainsoni was expanded (Leopardus geoffroyi, Carenivora, Felidae; and Calomys sp., Rodentia, Cricetidae), showing a wide host range for this species.

scholarly journals Morphological characterization of pre- and peri-implantation in vitro cultured, somatic cell nuclear transfer and in vivo derived ovine embryos

Reproduction ◽  
2005 ◽  
Vol 130 (5) ◽  
pp. 681-694 ◽  
Author(s):  
P Tveden-Nyborg ◽  
T T Peura ◽  
K M Hartwich ◽  
S K Walker ◽  
P Maddox-Hyttel
Keyword(s):  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Morphological Characterization ◽  
Morphological Development ◽  
Embryonic Disc ◽  
Transmission Electron

The processes of cellular differentiation were studied in somatic cell nuclear transfer (SCNT), in vitro cultured (IVC) and in vivo developed (in vivo) ovine embryos on days 7, 9, 11, 13, 17 and 19. SCNT embryos were constructed from in vitro matured oocytes and granulosa cells, and IVC embryos were produced by in vitro culture of in vivo fertilized zygotes. Most SCNT and IVC embryos were transferred to recipients on day 6 while some remained in culture for day 7 processing. In vivo embryos were collected as zygotes, transferred to intermediate recipients and retransferred to final recipients on day 6. All embryos were processed for examination by light and transmission electron microscopy or immunohistochemical labelling for alpha-1-fetoprotein and vimentin. Overall, morphological development of in vivo embryos was superior to IVC and SCNT embryos. Day 7 and particularly day 9 IVC and SCNT embryos had impaired hypoblast development, some lacking identifiable inner cell masses. On day 11, only in vivo and IVC embryos had developed an embryonic disc, and gastrulation was evident in half of in vivo embryos and one IVC embryo. By day 13, all in vivo embryos had completed gastrulation whereas IVC and SCNT embryos remained retarded. On days 17 and 19, in vivo embryos had significantly more somites and a more developed allantois than IVC and SCNT embryos. We conclude that IVC and particularly SCNT procedures cause a retardation of embryo development and cell differentiation at days 7–19 of gestation.

scholarly journals Genomic analysis and morphological characterization of bacteriophages for Salmonella spp. treatment in poultry

2018 ◽  
Author(s):  
Aline Parolin Calarga ◽  
Marcelo Brocchi
Keyword(s):  
Therapeutic Potential ◽  
Genomic Analysis ◽  
Storage Period ◽  
Public Health Problem ◽  
Morphological Characterization ◽  
Major Public Health Problem ◽  
Salmonella Spp ◽  
C Elegans

Enteric infections caused by Salmonella spp. represent a major public health problem worldwide, due to the large proportion of foodborne infections derived from this pathogen. Currently, antimicrobials are used to prevent contamination of chicken meat. However, in order to combat salmonellosis without the propagation of resistant strains, it is necessary to study alternative therapeutic approaches, such as the use of bacteriophages against Salmonellosis. For the present work bacteriophages provided by FMRP-USP were selected to further studies on its therapeutic potential. In addition, we work with lytic bacteriophages induced from monophasic strains of Salmonella spp. Our initial aim for this project was the morphological and molecular characterization of these viruses. Nevertheless, the environmental phages did not survive the storage period. Due to these results, our further studies will be focused on the lytic phages. They will be tested in vivo in the C. elegans model in order to evaluate the survival rate of the worms when infected with Salmonella spp.

scholarly journals IN VIVO AND IN VITRO STUDIES ON MORPHOLOGICAL CHARACTERIZATION OF SEPTORIA LYCOPERSICI CAUSING LEAF SPOT DISEASE IN TOMATO

2021 ◽  
Vol 21 (no 1) ◽  
Author(s):  
Aabid . ◽  
Sabiha Ashraf ◽  
Hilal A. Malik ◽  
Rakshanda Zargar ◽  
Shaheena A. Nagoo ◽  
...  
Keyword(s):  
Leaf Spot ◽  
Morphological Characterization ◽  
Leaf Spot Disease ◽  
Potato Dextrose Agar Medium ◽  
Spot Disease ◽  
Spore Mass ◽  
Septoria Lycopersici

Septoria lycopersici responsible for Septoria leaf spot disease was observed on the leaves of tomato. Septoria lycopersici was isolated and completion of Koch’s postulates confirmed that the fungus was causal agent of the leaf spot disease. The fungus was cultured on potato dextrose agar medium. The fungus was very slow growing with 8-12 mm radial growth as recorded after 30 days of incubation. The fungus produced off white, irregular, hardened blackish mycelial growth oozing spore mass from pycnidia. Pycnidia were dark brown to black, globose to sub globose, ostiolated and thick walled. Pycnidiospores were filiform, straight with pointed to rounded ends.

scholarly journals Distribution and Characterization of Monilinia spp. Causing Apple Fruit Decay in Serbia

Plant Disease ◽  
2018 ◽  
Vol 102 (2) ◽  
pp. 359-369 ◽  
Author(s):  
Miljan Vasić ◽  
Ivana Vico ◽  
Wayne M. Jurick ◽  
Nataša Duduk
Keyword(s):  
Pathogen Detection ◽  
Management Strategies ◽  
Brown Rot ◽  
Morphological Characterization ◽  
Apple Fruit ◽  
Postharvest Disease ◽  
Pome Fruit

Brown rot, caused by Monilinia spp., is an economically important pre- and postharvest disease of pome and stone fruits worldwide. In Serbia, apple is the most widely grown pome fruit, and the distribution of economically important Monilinia spp. responsible for apple brown rot is unknown. Hence, we conducted a three year survey, from 2010 to 2012, where 349 isolates were obtained from six orchards and four storage facilities from five different apple cultivars with brown rot symptoms. Morphological characterization of the isolates, multiplex PCR, and phylogenetic analysis revealed four species: M. fructigena, M. laxa, M. fructicola, and Monilia polystroma. All species were found in the orchard and in storage, with M. fructigena predominating, followed by M. polystroma. Representative isolates were analyzed in vitro and in vivo where differences in growth rate, sporulation, and virulence on apple fruit were observed. Findings from this investigation demonstrate diversity in the species responsible for pre- and postharvest apple brown rot, which has significant implications for pathogen detection and for developing disease-specific management strategies.

Morphological characterization of a new human epithelioid sarcoma cell line, ES020488, in vitro and in vivo

1993 ◽  
Vol 63 (1) ◽  
pp. 219-225 ◽  
Author(s):  
Hiroshi Sonobe ◽  
Mutsuo Furihata ◽  
Jun Iwata ◽  
Takashi Oka ◽  
Yuji Ohtsuki ◽  
...  
Keyword(s):  
Cell Line ◽  
Epithelioid Sarcoma ◽  
Morphological Characterization ◽  
Sarcoma Cell ◽  
Sarcoma Cell Line

Morphological characterization of Streptocephalus sirindhornae (Branchiopoda: Anostraca) from South East Asia: First record of the Streptocephalidae from China

Zootaxa ◽  
2015 ◽  
Vol 3911 (3) ◽  
pp. 447 ◽  
Author(s):  
Shusen Shu ◽  
Alejandro M. Maeda-Martinez ◽  
D. Christopher Rogers ◽  
Junxing Yang ◽  
Xiaoyong Chen
Keyword(s):  
East Asia ◽  
First Record ◽  
Morphological Characterization ◽  
South East Asia

Molecular and morphological characterization of Pristionchus pacificus (Nematoda: Rhabditida: Neodiplogastridae), a new record of an entomophilic nematode from Iran

Biologia ◽  
2013 ◽  
Vol 68 (5) ◽  
Author(s):  
Mahnaz Hassani-Kakhki ◽  
Javad Karimi ◽  
Ebrahim Shokoohi
Keyword(s):  
First Record ◽  
Morphological Characterization ◽  
The Body ◽  
Phylogenetic Position ◽  
Rdna Genes ◽  
Pristionchus Pacificus ◽  
The Family ◽  
Identification Measurements ◽  
Morphometric Characterization

AbstractDuring a survey of entomopathogenic nematodes from the Mashhad region, in northeastern Iran, an entomophilic nematode was isolated using the Galleria trap method. Morphological characterization showed that this isolate belongs to the family Neodiplogastridae. Detailed morphological and morphometric characterization including SEM data fit with Pristionchus pacificus Sommer, Carta, Kim et Sternberg, 1996, representing the first record of this genus and species from Iran. Iranian specimens of P. pacificus FUM 5 are characterized by the body length (0.69–0.83 mm), the stoma (6–10 μm) bearing a dorsal tooth and a conical elongated tail (146–220 μm). Molecular analyses using 18S and ITS rDNA genes further support this identification. Measurements, illustrations, and SEM photographs of the Iranian isolate are provided and the phylogenetic position of the species is discussed.

scholarly journals First record of expansive Ceratium Schrank, 1793 species (Dinophyceae) in Southern Brazil, with notes on their dispersive patterns in Brazilian environments

Check List ◽  
2013 ◽  
Vol 9 (4) ◽  
pp. 862 ◽  
Author(s):  
Kaoli Pereira Cavalcante ◽  
Juliana Conte Zanotelli ◽  
Carla Cristine Müller ◽  
Karen Dornelles Scherer ◽  
Juliana Karl Frizzo ◽  
...  
Keyword(s):  
First Record ◽  
South American ◽  
Southern Brazil ◽  
Rio Grande Do Sul ◽  
Dispersal Patterns ◽  
The Past ◽  
First Time ◽  
Freshwater Environments ◽  
South Brazil ◽  
Sem Analyses

Ceratium Schrank is a planktonic dinoflagellate ubiquitous in temperate and subtropical freshwater environments from Northern Hemisphere. Over the past two decades, Ceratium species have been recorded in South American water bodies, with expansive behavior and fast colonization. This study registered C. furcoides (Levander) Langhans and C. hirundinella (O. F. Müller) Dujardin for the first time in South Brazil. Ceratium furcoides was found in samples from States of Paraná and Rio Grande do Sul and C. hirundinella occurred only in the southernmost Brazil. No co-occurrence of these species was detected on samples. The morphological variation, as well as the dispersal patterns of these species in Brazilian environments, is discussed based on LM and SEM analyses.

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