The genus Diaphanosoma (Diplostraca: Sididae) in Greece: morphological and molecular assessment

Zootaxa ◽  
2021 ◽  
Vol 5082 (6) ◽  
pp. 572-582
Author(s):  
RAFAILIA ALEXIOU ◽  
GEORGIA STAMOU ◽  
STYLIANI MINOUDI ◽  
FILIO TOURLI ◽  
VALENTINA TSARTSIANIDOU ◽  
...  
Keyword(s):  
Type Locality ◽  
Coi Gene ◽  
Sample Analysis ◽  
Genetic Analyses ◽  
Molecular Identity ◽  
Extra Effort ◽  
Mtdna Coi ◽  
Greek Lakes ◽  
Molecular Assessment ◽  
Genetic Results

For the genus Diaphanosoma Fischer (Cladocera) the species’ name “D. brachyurum” has been widely used for many decades to identify other species belonging to this genus. To clarify the diversity of the genus in Greek lakes in the present study, we morphologically and genetically identified the Diaphanosoma species occurring in eight lakes. Three hundred twenty-nine Diaphanosoma individuals were morphologically examined, while for the genetic analyses the mtDNA COI gene was sequenced in 48 individuals. Combining the morphological and genetic results, we verified the occurrence of D. mongolianum, D. orghidani and D. macedonicum in our study area. We could not confirm prior records of D. brachyurum and D. lacustris while we provide the molecular identity of D. macedonicum. Furthermore, we highlight the need to check whether the European D. mongolianum populations are characterised of mitochondrial discordance and hybridization as the individuals from the Asian type locality of the species. Our results support the importance of combining both approaches to correctly identify taxonomic species, despite the extra effort and cost during the sample analysis.  

Taxonomic reassessment of the freshwater mussel genus Unio (Bivalvia: Unionidae) in Russia and Ukraine based on morphological and molecular data

Zootaxa ◽  
2017 ◽  
Vol 4286 (1) ◽  
pp. 93 ◽  
Author(s):  
OLGA KLISHKO ◽  
MANUEL LOPES-LIMA ◽  
ELSA FROUFE ◽  
ARTHUR BOGAN ◽  
LYUDMILA VASILIEV ◽  
...  
Keyword(s):  
Freshwater Mussel ◽  
Integrated Approach ◽  
Molecular Data ◽  
Coi Gene ◽  
Data Set ◽  
Widespread Species ◽  
Molecular Analyses ◽  
Morphological Variations ◽  
Mtdna Coi ◽  
Morphological And Molecular Data

The taxonomy of species within the genus Unio (Bivalvia: Unionidae: Unioninae) in Russia and Ukraine has been contentious due to the lack of correspondence between three concurrent yet divergent classifications. In order to clarify which classification system best reflects the evolutionary relationships among these taxa, we performed detailed morphological analyses on 720 Ukrainian and Russian specimens, complemented with molecular data (COI) from a selected number of specimens. The morphological character data set shows the existence of only three widespread species with slight eco-morphological variations. Statistical analyses of shell morphometric parameters and molecular analyses based on mtDNA COI gene fragment sequences confirm the existence of the same three species within a single genus, Unio, in Russia and Ukraine, that is U. pictorum, U. tumidus and U. crassus. Results from molecular analyses suggest the existence of an additional subgroup within the U. crassus lineage, U. crassus cf. courtilieri that deserves further research. The present integrated approach confirms the validity of the classification by Zhadin (1952) and rejects the complex classifications of Starobogatov et al. (2004) and Bogatov & Kijashko (2016). 

Identification and pathogenicity of Pratylenchus scribneri on tomato in Sichuan Province of People's Republic of China

2022 ◽  
Vol 96 ◽  
Author(s):  
Y.H. Xia ◽  
J. Li ◽  
M.R. Sun ◽  
B. Lei ◽  
H.L. Li ◽  
...  
Keyword(s):  
Sichuan Province ◽  
28S Rdna ◽  
Coi Gene ◽  
Root Lesion Nematode ◽  
Republic Of China ◽  
Mtdna Coi ◽  
Lesion Nematodes ◽  
Lesion Nematode ◽  
Pratylenchus Scribneri ◽  
Root Lesion Nematodes

Abstract Root-lesion nematodes (Pratylenchus spp.) are a group of economically important pathogens that have caused serious economic losses in many crops. In 2019, root-lesion nematodes were recovered from tomato (Solanum lycopersicum) root samples collected from Sichuan Province, People's Republic of China (PRC). Extracted nematodes were disinfected, and one individual female was cultured on a carrot disc for propagation at 25 °C by parthenogenesis and designated the SC isolate. Afterwards, the isolate was identified on the basis of morphometric and molecular markers. Both morphometric characters and molecular analysis of the internal transcribed spacer region gene (ITS) of ribosomal DNA, the D2-D3 expansion region of the 28S rDNA gene and the mitochondrial cytochrome oxidase I (mtDNA-COI) gene revealed that the species of root-lesion nematode was Pratylenchus scribneri. The Bayesian tree inferred from the ITS rDNA, 28S rDNA and mtDNA-COI gene sequences also showed that this isolate formed a highly supported clade with other P. scribneri isolates. The pathogenicity of the root-lesion nematode SC isolate on tomato was assessed, showing that tomato was a suitable host for P. scribneri. To the best of our knowledge, this is the first report of P. scribneri on tomato in Sichuan Province, PRC. These are also the first molecular data obtained from P. scribneri on tomato in the PRC, and the pathogenicity of P. scribneri to tomato was studied for the first time. This study provides scientific data for the detection, identification and control of tomato root-lesion nematode disease.

Description of two new Australian species of Abantiades Herrich-Schäffer (Lepidoptera: Hepialidae) and females of two further species with notes on their biogeography 

Zootaxa ◽  
2020 ◽  
Vol 4822 (1) ◽  
pp. 71-93
Author(s):  
MICHAEL D. MOORE ◽  
ETHAN P. BEAVER ◽  
ALEJANDRO VELASCO-CASTRILLÓN ◽  
MARK I. STEVENS
Keyword(s):  
Arid Regions ◽  
Common Ancestor ◽  
Species Recognition ◽  
Species Distributions ◽  
Coi Gene ◽  
Dna Barcodes ◽  
Coastal Regions ◽  
Late Mesozoic ◽  
Australian Species ◽  
Mtdna Coi

Abantiades cephalocorvus sp. nov. and Abantiades tembyi sp. nov. are described, along with the previously undescribed females of A. macropusinsulariae Simonsen, 2018 and A. pallida Simonsen, 2018. All of these species belong to a triforked Abantiades Herrich-Schäffer clade that is loosely centred around the Nullarbor and other arid regions of Australia. We explore DNA barcodes (mtDNA COI gene) from these and other Abantiades and discuss their significance for species recognition. The species distributions are entirely or largely allopatric and we discuss their origins from a widespread common ancestor that was likely distributed over inland and coastal regions in the mid- to late-Mesozoic before the onset of desertification. Notes on new distributional data for all of the known species in this clade are included. 

A morphometric approach supporting genetic results in the taxonomy of the New Zealand limpets of the Cellana strigilis complex (Mollusca : Patellogastropoda : Nacellidae)

2012 ◽  
Vol 26 (2) ◽  
pp. 193 ◽  
Author(s):  
Céline M. O. Reisser ◽  
Bruce A. Marshall ◽  
Jonathan P. A. Gardner
Keyword(s):  
New Zealand ◽  
Red Sea ◽  
Morphometric Analysis ◽  
Type Locality ◽  
Group A ◽  
Eastern Species ◽  
Genetic Results

The New Zealand Cellana strigilis complex has been traditionally divided into six subspecies. Recent molecular investigations, however, revealed that the complex comprises two clades. In this paper, an additional morphometric analysis on 160 shells from the two clades confirms the need for taxonomic reconsideration of the C. strigilis complex. Here, two species are recognised in the Cellana strigilis group, a western species, C. strigilis (Hombron & Jacquinot, 1841), from South, Stewart, Snares, Auckland and Campbell islands, with Patella redimiculum Reeve, 1854 and C. strigilis flemingi Powell, 1955 as synonyms; and an eastern species, C. oliveri Powell, 1955, from Chatham, Bounty Islands and Antipodes Islands, with C. strigilis bollonsi Powell, 1955 and C. chathamensis of authors (not Pilsbry, 1891) as synonyms. Acmaea chathamensis Pilsbry, 1891 is based on mislocalised foreign shells, probably C. rota (Gmelin, 1791) from the Red Sea. A neotype is designated for P. strigilis Hombron & Jacquinot, 1841, lectotypes are designated for P. redimiculum Reeve, 1854 and A. chathamensis Pilsbry, 1891, and a type locality is selected for P. redimiculum.

scholarly journals Study of mtDNA COI Gene Polymorphism and Relationship With Some Productive Traits in Awassi Sheep

2021 ◽  
Vol 910 (1) ◽  
pp. 012002
Author(s):  
Ahmed Ismael Khalaf ◽  
Nasr Noori Al-Anbari
Keyword(s):  
Gene Polymorphism ◽  
Milk Yield ◽  
Growth Traits ◽  
Scientific Progress ◽  
Coi Gene ◽  
Research Station ◽  
Sequencing Analysis ◽  
Lactation Period ◽  
Mtdna Coi ◽  
Awassi Sheep

Abstract This study was carried out in two locations (the field part), first in the Ruminants Researches Station (20 km west of Baghdad), and the second in the Aldawar research station located in Anbar governorate (Up to 35 km west of Ramadi city), which included 87 animals from Awassi sheep. As for the laboratory part, it took place in two locations, the first in the Animal Breeding and Improvement Laboratory of the Ruminant Research Department, and the second in the Scientific Progress Laboratory/Baghdad. This study aimed to investigate the relationship between polymorphism of mtDNA-COI gene and growth traits, milk yield, and lactation period in Awassi sheep. DNA was extracted from the blood and the polymerase Chain Reaction (PCR) technique was used to amplify the COI gene. DNA sequencing analysis. The results showed that there are 6 SNPs in a COI gene, there polymorphism for this gene also appeared (TT, CC). The 1st site (5566), CC polymorphism achieved the highest percentages (39.09) compared with TT polymorphism, with an allelic frequency of 0.57. While it appeared in the 2nd, 3rd, 4th, and 6th sites the polymorphism TT and CC with percentages (79.31 and 20.69)%, (58.42 and 41.38)%, (20.69 and 79.31), (79.31 and 20.69)%, and with the allele frequency (0.79 and 0.21), (0.59 and 0.41), (0.21 and 0.79), (0.79 and 0.21) respectively. there were significant differences (P<0.05) of COI polymorphism in birth weight, weaning weight and gain from birth to weaning as the TT recorded the highest average for both traits (3.82, 25.39 and 21.57 kg respectively). The mean of daily milk yield for TT polymorphism was 330.21 gm/day, which significant (P<0.05) the CC polymorphism, no significant between COI polymorphism and lactation period. It was possible to conclude from this study the possibility of COI gene polymorphism in the development of genetic improvement strategies and breeding programs that achieved the best productive performance sheep.

scholarly journals New insight into the taxonomic resolution of the genus Bythotrephes Leydig (Crustacea: Cladocera) based on molecular data from Central Europe

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Maciej Karpowicz ◽  
Magdalena Świsłocka ◽  
Joanna Moroz ◽  
Łukasz Sługocki
Keyword(s):  
Genetic Analysis ◽  
Central Europe ◽  
Taxonomic Status ◽  
Molecular Data ◽  
Coi Gene ◽  
Taxonomic Resolution ◽  
Monotypic Genus ◽  
Mtdna Coi ◽  
Xix Century ◽  
Insight Into

AbstractThe taxonomic status of the genus Bythotrephes Leydig (Crustacea: Cladocera) has been debated since the second half of the XIX century. The most widespread view of recent decades has been that Bythotrephes is a monotypic genus, which was support by preliminary molecular data. However, the recent detailed morphological revision of this genus clearly distinguishes at least seven species. Therefore, we performed a multi-lake survey in Central Europe to give new insight into the taxonomic status of Bythotrephes by combining genetic analysis with traditional morphology-based taxonomy. Based on the morphology we identified two species in Central Europe, B. brevimanus and B. lilljeborgi, as well as hybrid forms. For the genetic analysis, we used newly obtained 113 sequences of mtDNA COI gene of the 535-bp length Bythotrephes from Central Europe and sequences downloaded from GenBank. There were no significant differences between all analyzed sequences, which supports the hypothesis that Bythotrephes is a monotypic genus, with only one highly polymorphic species. On the other hand, the results of our work could point out that the COI gene is insufficient to evaluate the taxonomic status of Bythotrephes. Nonetheless, we have identified 29 new haplotypes of mtDNA COI, and one which was the same as the haplotype found in North America and Finland. Furthermore, this haplotype was the source variant from which most other haplotypes were derived.

scholarly journals Ultrastructure characters and partial mtDNA-COI haplotypes of Asian corn borer, Ostrinia furnacalis (Guenée) (Lepidoptera: Crambidae) from Indonesia

2020 ◽  
Vol 21 (7) ◽  
Author(s):  
Ahmad Taufiq Arminudin ◽  
Suputa ◽  
Arman Wijonarko ◽  
Y. Andi Trisyono
Keyword(s):  
The Philippines ◽  
Coi Gene ◽  
Ostrinia Furnacalis ◽  
High Similarity ◽  
Microscopy Observation ◽  
Corn Borer ◽  
Mtdna Coi ◽  
Asian Corn Borer ◽  
Relationship Of ◽  
The Relationship

Abstract. Arminudin AT, Suputa, Wijonarko A, Trisyono YA. 2020. Ultrastructure characters and partial mtDNA-COI haplotypes of Asian corn borer, Ostrinia furnacalis (Guenée) (Lepidoptera: Crambidae) from Indonesia. Biodiversitas 21: 2914-2922. In addition to the confirmation of the species of corn borer, we analyzed the relationship of Ostrinia furnacalis in Indonesia with that in other countries. Moths of O. furnacalis were collected from several areas in Java and Sumatra islands of Indonesia. Forewing and labial palpi scales were investigated under Scanning Electron Microscopy observation. A 658 bp of O. furnacalis partial COI gene sequences of 1480-2138 nucleotides were downloaded from GenBank and BOLD system databases. The ridge lamellae and window characters as unique ultrastructure characters of scale were further supported by the COI gene analysis. High similarity was observed between the Java and the Philippines specimens, but they were a different haplotype compared with Sumatra’s specimen. The results could provide the baseline data on the genetic variation of O. furnacalis in Indonesia.

Sign in / Sign up

Export Citation Format

Share Document