Size-Dependent Biological Activities of Fluorescent Organosilane-Modified Zinc Oxide Nanoparticles

2020 ◽  
Vol 16 (2) ◽  
pp. 137-152
Author(s):  
Mariana Buşilă ◽  
Aurel Tăbăcaru ◽  
Viorica Muşsat ◽  
Bogdan Ştefan Vasile ◽  
Ionela Andreea Neaşu ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Zinc Oxide ◽  
Cancer Cells ◽  
Zinc Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Cytotoxic Activities ◽  
Zno Nps ◽  
Size Dependent ◽  
Transmission Electron

Surface modification of zinc oxide nanoparticles (ZnO NPs) is a strategy to tune their biocompatibility. Herein we report on the synthesis of a series of fluorescent ZnO NPs modified with 2–10% (3-glycidyloxypropyl)trimethoxysilane (GPTMS) to investigate the fluorescence properties and to explore their applications in microbiology and biomedicine. The obtained ZnO NPs were characterized by X-ray diffraction (XRD), high resolution transmission electron microscopy (HRTEM) and Fourier transform infrared spectroscopy (FTIR). Size reduction occurred from ca. 13 nm in unmodified ZnO to 3–4 nm in silane-modified samples and fluorescence spectra showed size-dependent variation of the photoemission bands' intensity. The antibacterial and cytotoxic activities were investigated on Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria, and in ovarian (A2780) and prostate (PC3) cancer cells by tetrazolium/formazan-based methods. The antibacterial effect was higher for E. coli than S. aureus, while the cytotoxic activity was similar for both cancer cells and varied with the particle size. Cell death by apoptosis, and/or necrosis versus autophagy, were explored by flow cytometry using an Annexin V based-method and transmission electron microscopy (TEM). The main mechanism of ZnO NPs toxicity may involve the generation of reactive oxygen species (ROS) and the induction of apoptosis or autophagy. This work revealed the potential utility of GPTMS-modified ZnO NPs in the treatment of bacterial infection and cancer.

Preparation, structural characterization, and gas separation properties of functionalized zinc oxide particle filled poly(ether-amide) nanocomposite films

2016 ◽  
Vol 33 (1) ◽  
pp. 92-113 ◽  
Author(s):  
Sajad Shojai Nasab ◽  
Saeed Zahmatkesh
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Zinc Oxide ◽  
Thermal Gravimetric Analysis ◽  
Zinc Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Gravimetric Analysis ◽  
Thermal Gravimetric ◽  
Transmission Electron ◽  
Surface Modified

In this study, a new diacid monomer containing amide, imide, and sulfone functional groups was successfully used to synthesize a new poly(ether-amide) for membrane-based gas separation applications. The synthesized poly(ether-amide) was soluble in organic solvents, has high thermal stability (up to 460℃ under nitrogen atmosphere, for 10% weight loss), and high glass transition temperature (Tg = 274℃). This poly(ether-amide) was combined with different amounts of surface-modified zinc oxide nanoparticles to provide organic–inorganic nanocomposites. The optically transparent and flexible membranes of these hybrid nanocomposites were prepared. The obtained materials were characterized by Fourier transform-infrared spectroscopy, thermal gravimetric analysis, differential scanning calorimetry, X-ray powder diffraction, field emission-scanning electron microscopy, and transmission electron microscopy techniques. Transmission electron microscopy of the nanocomposite film with 15 wt% zinc oxide confirms that the nanoparticles are well dispersed in the polymer matrix. Thermal gravimetric analysis data indicated that the hybrid materials had better thermal behavior with increasing surface-modified zinc oxide nanoparticles nanoparticle content. The poly(ether-amide)/surface-modified zinc oxide nanoparticles nanocomposite film mechanical properties improved with increasing surface-modified zinc oxide nanoparticles content. The permeability and selectivity of the poly(ether-amide)/zinc oxide membranes as a function of the surface-modified zinc oxide nanoparticles weight percentage were studied, and the results indicated that the CO2 and CH4 permeability increased with increasing zinc oxide content. In general, the membranes prepared from these polymers showed very good permeability and permselectivity for a pair of gases.

Quenching of Growth of Zinc Oxide Nanoparticles by Adsorption of Organic Stabilizers

2004 ◽  
Vol 449-452 ◽  
pp. 1133-1136 ◽  
Author(s):  
Dong Un Seo ◽  
Cheng Zhu Lu ◽  
Ho Jung Chang ◽  
Sang Woo Joo
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Zinc Oxide ◽  
Zno Nanoparticles ◽  
Zinc Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Zno Nanoparticle ◽  
Photoluminescence Spectra ◽  
Transmission Electron ◽  
Microscopy Images

We examined quenching of growth of ZnO nanoparticles with the injection of various organic surfactants. Aliphatic isocyanides as well as organothiols were found to adsorb on ZnO nanoparticle surfaces. For a carboxylate-terminated thiol, a retardation of growth appeared to be effective presumably due to the adsorption of the carboxylate COO- group on the ZnO surface. Photoluminescence spectra and transmission electron microscopy images were obtained for thiolcapped ZnO nanoparticles. The thiol-capped ZnO nanoparticles was found to fluoresce at the wavelength shorter by ~6 nm than those in the absence of any organic surfactants.

scholarly journals Biosynthesis of Zinc Oxide Nanoparticles from Acacia nilotica (L.) Extract to Overcome Carbapenem-Resistant Klebsiella Pneumoniae

Molecules ◽  
2021 ◽  
Vol 26 (7) ◽  
pp. 1919
Author(s):  
Elsayim Rasha ◽  
AlOthman Monerah ◽  
Alkhulaifi Manal ◽  
Ali Rehab ◽  
Doud Mohammed ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Zinc Oxide ◽  
Klebsiella Pneumoniae ◽  
Zinc Oxide Nanoparticles ◽  
Acacia Nilotica ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
X Ray ◽  
Carbapenem Resistant

Recently, concerns have been raised globally about antimicrobial resistance, the prevalence of which has increased significantly. Carbapenem-resistant Klebsiella pneumoniae (KPC) is considered one of the most common resistant bacteria, which has spread to ICUs in Saudi Arabia. This study was established to investigate the antibacterial activity of biosynthesized zinc oxide nanoparticles (ZnO-NPs) against KPC in vitro and in vivo. In this study, we used the aqueous extract of Acacia nilotica (L.) fruits to mediate the synthesis of ZnO-NPs. The nanoparticles produced were characterized by UV-vis spectroscopy, zetasizer and zeta potential analyses, X-ray diffraction (XRD) spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and transmission electron microscopy (TEM). The antimicrobial activity of ZnO-NPs against KPC was determined via the well diffusion method, and determining minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), the results showed low MIC and MBC when compared with the MIC and MBC of Imipenem and Meropenem antibiotics. The results of in vitro analysis were supported by the results upon applying ZnO-NP ointment to promote wound closure of rats, which showed better wound healing than the results with imipenem ointment. The biosynthesized ZnO-NPs showed good potential for use against bacteria due to their small size, applicability, and low toxicity to human cells.

scholarly journals Bioactivities of Geranium wallichianum Leaf Extracts Conjugated with Zinc Oxide Nanoparticles

Biomolecules ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 38 ◽  
Author(s):  
Banzeer Ahsan Abbasi ◽  
Javed Iqbal ◽  
Riaz Ahmad ◽  
Layiq Zia ◽  
Sobia Kanwal ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Energy Dispersive ◽  
Oxide Nanoparticles ◽  
X Ray Diffraction ◽  
Leaf Extracts ◽  
Tem Analysis ◽  
X Ray ◽  
Transmission Electron

This study attempts to obtain and test the bioactivities of leaf extracts from a medicinal plant, Geranium wallichianum (GW), when conjugated with zinc oxide nanoparticles (ZnONPs). The integrity of leaf extract-conjugated ZnONPs (GW-ZnONPs) was confirmed using various techniques, including Ultraviolet–visible spectroscopy, X-Ray Diffraction, Fourier Transform Infrared Spectroscopy, energy-dispersive spectra (EDS), scanning electron microscopy, transmission electron microscopy, and Raman spectroscopy. The size of ZnONPs was approximately 18 nm, which was determined by TEM analysis. Additionally, the energy-dispersive spectra (EDS) revealed that NPs have zinc in its pure form. Bioactivities of GW-ZnONPs including antimicrobial potentials, cytotoxicity, antioxidative capacities, inhibition potentials against α-amylase, and protein kinases, as well as biocompatibility were intensively tested and confirmed. Altogether, the results revealed that GW-ZnONPs are non-toxic, biocompatible, and have considerable potential in biological applications.

SIZED-CONTROLLED ZnO NANOPARTICLES, SYNTHESIS AND MORPHOLOGY

2009 ◽  
Vol 08 (03) ◽  
pp. 277-279 ◽  
Author(s):  
MOHSEN OFTADEH ◽  
MASOUD SALAVATI-NIASARI ◽  
FATEMEH DAVAR
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fourier Transform ◽  
Zinc Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
X Ray Diffraction ◽  
X Ray ◽  
Transmission Electron ◽  
Zinc Acetylacetonate ◽  
Nanoparticles Synthesis

Zinc oxide nanoparticles were successfully prepared through the decomposition of zinc acetylacetonate precursor in oleylamine in the presence of triphenylphosphine. The products were characterized by X-ray diffraction, transmission electron microscopy, and Fourier transform infrared spectroscopy.

scholarly journals Greener Synthesis of Zinc Oxide Nanoparticles: Characterization and Multifaceted Applications

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4198
Author(s):  
Ali Aldalbahi ◽  
Seham Alterary ◽  
Ruba Ali Abdullrahman Almoghim ◽  
Manal A. Awad ◽  
Noura S. Aldosari ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Zinc Oxide ◽  
Cell Line ◽  
Zinc Oxide Nanoparticles ◽  
Diffusion Method ◽  
Oxide Nanoparticles ◽  
Eosin Y ◽  
Zno Nps ◽  
Vitro Assay ◽  
Vis Spectroscopy

Nanoparticles (NPs) have unique properties compared to their bulk counterparts, and they have potentials for various applications in many fields of life science. Green-synthesized NPs have garnered considerable interest due to their inherent features such as rapidity, eco-friendliness and cost-effectiveness. Zinc oxide nanoparticles (ZnO NPs) were synthesized using an aqueous extract of Kalanchoe blossfeldiana as a reducing agent. The resulting nanoparticles were characterized via X-ray diffraction (XRD), dynamic light scattering (DLS), UV-Vis spectroscopy, photoluminescence (PL), transmission electron microscopy (TEM), scanning electron microscopy (SEM) and energy-dispersive spectroscopy (EDS). The antimicrobial potential of the synthesized ZnO NPs against bacterial and fungal strains was examined by the disk diffusion method, and they showed a promising antibacterial and antifungal potential. The catalytic activity of the synthesized ZnO NPs in reducing methylene blue (MB) and eosin was studied via UV-Vis spectroscopy. The decolorization percentages of the MB and Eosin Y dyes were 84% and 94%, respectively, which indicate an efficient degradation of the ZnO NPs. In addition, the cytotoxic activity of the ZnO NPs on the HeLa cell line was evaluated via in vitro assay. The MTT assay results demonstrate a potent cytotoxic effect of the ZnO NPs against the HeLa cancer cell line.

scholarly journals Laboratory and Commercial Synthesized Zinc Oxide Nanoparticles Adsorption onto Coconut Husk: Characterization, Isotherm, Kinetic, and Thermodynamic Studies

2020 ◽  
Vol 11 (1) ◽  
pp. 7871-7889
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Batch Adsorption ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Maximum Capacity ◽  
Maximum Percentage ◽  
Coconut Husk ◽  
Transmission Electron ◽  
Percentage Removal

The accelerating application of zinc oxide nanoparticles (ZnO-NPs) has called for attention to their potential environmental and human health risks. This work aimed to investigate the sorption efficiency of laboratory and commercial synthesized nanocrystalline zinc oxide onto raw coconut husk in a batch adsorption study. Characterization of samples was performed by employing spectroscopies techniques such as X-ray Diffraction Spectroscopy, Field Emission Scanning Electron Microscopy, Transmission Electron Spectroscopy, Fourier transform IR Spectroscopy, and Brunauer–Emmett–Teller. A spherical shaped nanocrystalline ZnO with a mean crystallite and particle size of 14.7 nm and 24 nm by XRD and TEM was synthesized as compared to the commercial ZnO-NPs of size < 50 nm. The maximum percentage removal of 88% (0.13 mg/g) and 90% (0.16 mg/g) for laboratory synthesized and commercial ZnO-NPs respectively was recorded at an optimum contact time of 80 minutes. The data also indicated 2.0 g sorbent mass and pH of 8 as the optimum conditions for maximum percentage removal of these nanoparticles. Both Langmuir and Freundlich models fitted best for laboratory synthesized ZnO-NPs with a maximum capacity of 0.797 mg/g, whereas Langmuir isotherm model alone with a maximum capacity of 0.710 mg/g fitted well for commercial ZnO-NPs. The n-value from the Freundlich model, as well as separation factor (RL) were greater than unity suggesting a favorable adsorption process. The study obeyed pseudo-second-order, which was exothermic with a high degree of freedom of sorbent-sorbate interaction. The results suggested that coconut husk is potentially scalable for removing ZnO-NPs from wastewater.

scholarly journals Cytotoxic effects of zinc oxide nanoparticles on cyanobacteriumSpirulina (Arthrospira) platensis

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4682 ◽  
Author(s):  
Sinouvassane Djearamane ◽  
Yang Mooi Lim ◽  
Ling Shing Wong ◽  
Poh Foong Lee
Keyword(s):  
Electron Microscopy ◽  
Zinc Oxide ◽  
Cell Viability ◽  
Aquatic Environment ◽  
Zinc Oxide Nanoparticles ◽  
Algal Biomass ◽  
Time Dependent ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Cytotoxic Effects

BackgroundThe extensive usage of zinc oxide nanoparticles (ZnO NPs) in industrial and consumer products raises the risk of releasing their residues into the aquatic environment. The presence of ZnO NPs in the aquatic environment could potentially cause cytotoxic effects on aquatic organisms. Thus, investigating the cytotoxic effects of ZnO NPs on microalgae, which form the base for the food web of aquatic biota, is essential to gain information regarding the ecotoxicological effects of metallic oxide nanoparticles in the aquatic ecosystem. Therefore, the present study has investigated in detail the assorted cytotoxic effects of ZnO NPs onS. platensisusing various concentrations of ZnO NPs (10–200 mg/L) from 6 to 96 h to explore the dose- and time-dependent cytotoxic effects.MethodsThe cytotoxic effects were all assessed through quantification of loss in cell viability, reduction in biomass and decrease in photosynthetic pigments such as chlorophyll-a, carotenoids and phycocyanin. The surface interactions of nanoparticles and the subsequent morphological alterations on algal cells were examined by optical and scanning electron microscopy (SEM). The intracellular alterations of algal cells were studied using transmission electron microscopy. Furthermore, Fourier transformed infrared (FTIR) spectrum was obtained to investigate the involvement of algal surface biomolecules in surface binding of ZnO NPs on algal cells.ResultsThe treatment of ZnO NPs onS. platensisexhibited a typical concentration- and time-dependent cytotoxicity. Results showed a significant (p < 0.05) cytotoxicity from 24 h onwards for all tested concentrations of ZnO NPs. The maximum cytotoxicity on algal cells was achieved at 96 h of exposure to ZnO NPs. In comparison with control, the algal cells that interacted with 200 mg/L of ZnO NPs for 96 h showed 87.3 ± 1% loss in cell viability, 76.1 ± 1.7% reduction in algal biomass, 92.5 ± 2.2%, 76.2 ± 2.2% and 74.1 ± 3.4% decrease in chlorophyll-a, carotenoids and phycocyanin contents respectively. Our study confirmed the cytotoxicity of ZnO NPs through the algal growth inhibition with 72 h EC10and EC50values of 1.29 and 31.56 mg/L, respectively. The microscopic examinations of the algal cells that interacted with ZnO NPs showed severe cell membrane and intracellular damage. The SEM EDX spectrum of ZnO NPs treated algal biomass evidenced the surface accumulation of zinc in the biomass. Finally, the FTIR spectrum confirmed the involvement of amino, hydroxyl and carboxylic groups of algal cell wall in the surface interaction of ZnO NPs on the algal cells.DiscussionThe results showed that the treatment of ZnO NPs onS. platensistriggered substantial cytotoxicity and caused cell death. Hence,S. platensiscould be potentially used as a bioindicator for testing toxicity of ZnO NPs in aquatic environment.

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