Electrospun Nanofibers Embedded with Copper Oxide Nanoparticles to Improve Antiviral Function

Many studies on anti-bacterial/antiviral surfaces have been conducted to prevent epidemic spread worldwide. Several nanoparticles such as those composed of silver and copper are known to have antiviral properties. In this study, we developed copper oxide (CuO) nanoparticle-incorporated nanofibers to inactivate or remove viruses. The CuO nanoparticle-incorporated nanofiber was fabricated with a hydrophobic polymer—polyvinylpyrrolidone (PVP)—using electrospinning, and CuO nanoparticles were exposed from the PVP polymer surface by etching the nanofiber with oxygen plasma. The fabrication conditions of electrospinning and oxygen plasma etching were investigated by scanning electron microscopy (SEM), and field emission transmission electron microscopy (FETEM)/ energy dispersive spectrometry (EDS). H1N1 virus was utilized as the target sample and quantified by RT-qPCR. The antiviral efficacy of CuO nanoparticle-incorporated nanofibers was compared against bare CuO nanoparticles. Overall, 70% of the viruses were inactivated after CuO nanoparticle-incorporated nanofibers were incubated with 102 pfu/mL of H1N1 virus solution for 4 h. This indicates that the developed CuO nanoparticle-incorporated nanofibers have noticeable antiviral efficacy. As the developed CuO nanoparticle-incorporated nanofibers exerted promising antiviral effects against H1N1 virus, it is expected to benefit global health by preventing epidemic spread.

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Comparing study of CuO synthesized by biological and electrochemical methods for biological activity

Al-Mustansiriyah Journal of Science ◽

10.23851/mjs.v30i1.389 ◽

2019 ◽

Vol 30 (1) ◽

pp. 94 ◽

Cited By ~ 3

Author(s):

Shaimaa Hamed Jaber

Keyword(s):

Electron Microscopy ◽

Copper Oxide ◽

Electrochemical Method ◽

Cuo Nanoparticles ◽

Biological Method ◽

X Ray Diffraction ◽

X Ray ◽

Transmission Electron ◽

Average Size ◽

Cuo Nanoparticle

In the present work, copper oxide (CuO) nanoparticles have been synthesized by two methods (electro chemical and biological method).The synthesized nanoparticles characterized by x-ray diffraction (XRD), Scanning Electron Microscopy(SEM) and transmission Electron Microscopy(TEM). results show that to copper oxide (CuO) nanoparticle have average size of (11-15)nm of electrochemical method and (6-12 ) nm of biological method by different technique CuO nanoparticles were applied to study the inhibition of bacterial using (staphylococcus and pseudomonas). The antibacterial activity of CuO nanoparticles show a higher inhibition of pseudomonas bacteria when a compared with staphylococcus bacteria.

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Synthesis & Study of Nano Size Copper Oxide Particle via Chemical Method

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.829.187 ◽

2013 ◽

Vol 829 ◽

pp. 187-191

Author(s):

Maryam Abdolhosseinzadeh ◽

Niloofar Khodamoradi

Keyword(s):

Electron Microscopy ◽

Copper Oxide ◽

Oxide Particle ◽

Copper Oxide Nanoparticles ◽

Cuo Nanoparticles ◽

Molar Ratio ◽

Transmission Electron ◽

Uv Visible ◽

Micro Reactors ◽

Nano Size

Copper oxide nanoparticles have been synthesized using micro reactors made of bis (2-ethylhexyle) sulfosuccinate (AOT)/water/n-Hexane microemulsions. The controls of particles size was achieved by varying water to surfactant molar ratio (W0). At constant of surfactant concentration the increases in value of W0increased the population of micelles and resulted in lager particle size. Their sizes and appearance were characterized by TEM, SEM, Zetasizer, uv-visible methods. Scanning electron microscopy (SEM) showed the spherical morphology of as prepared CuO nanoparticles. The Transmission electron microscopy (TEM) showed 85 nm size of as prepared CuO particles.

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A facile approach towards copper oxide nanoparticles synthesis using Spirulina platensis and assessment of its biological activities

Brazilian Journal of Biological Sciences ◽

10.21472/bjbs.051020 ◽

2018 ◽

Vol 5 (10) ◽

pp. 433-442 ◽

Cited By ~ 1

Author(s):

Priyanga Jayakrishnan ◽

Sirajunnisa Abdul Razack ◽

Keerthana Sivanesan ◽

Pavithra Sellaperumal ◽

Geethalakshmi Ramakrishnan ◽

...

Keyword(s):

Copper Oxide ◽

Spirulina Platensis ◽

Biological Activities ◽

Nanoparticle Synthesis ◽

Cuo Nanoparticles ◽

Metal Nanoparticle ◽

Anticancer Activities ◽

Cytotoxicity Activity ◽

Ft Ir ◽

Cuo Nanoparticle

There is a budding need to develop a method for environmentally benign metal nanoparticle synthesis, that do not use toxic chemicals in the synthesis protocols to avoid adverse effects in medical applications. The present investigation dealt with the synthesis of copper oxide (CuO) nanoparticles from blue green alga, Spirulina platensis. The algal extract consisting of phytochemicals was used as the reducing agent and copper sulphate as the substrate. Synthesised nanoparticles were characterized by UV-Vis spectrophotometry, FT-IR spectroscopy, XRD and SEM. Antibacterial and anticancer activities were assessed for the CuO nanoparticles. The results indicated that the formed CuO nanoparticles were observed to be nanosheets. FT-IR spectral analysis elucidated the occurrence of biomolecules required for the reduction of copper oxide ions. The synthesized nanoparticles were found to be effective at the concentration of 1 mg/mL against Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa and Serratia marcescens. The cytotoxicity activity of CuO nanoparticle was evaluated by MTT Assay against colon cancer cell lines and confirmed that CuO nanoparticle at a concentration of 125 µg/mL had cytotoxic activity. In conclusion, the CuO nanoparticles were synthesized at a low energy supply, in an ecologically safe mode which could be utilized for pharmacological applications and various biotechnological studies.

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Biomimetic copper oxide nanoparticles and its validation through in-silico approach on cardiac enzymes

Current Nanoscience ◽

10.2174/1573413716666210101161139 ◽

2021 ◽

Vol 16 ◽

Author(s):

Jacquline Rosy P ◽

VenkatKumar Shanmugam ◽

Jebastin Sonia Jas ◽

Santhoshkumar Jayakodi

Keyword(s):

Electron Microscopy ◽

Copper Oxide ◽

In Silico ◽

Active Sites ◽

Computational Analysis ◽

Cuo Nanoparticles ◽

Oxide Nanoparticles ◽

Cardiac Enzymes ◽

Hydrogen Bond Interaction ◽

Cardioprotective Effects

Background: The present study outlines the green synthesis of copper oxide (GS-CuO) nanoparticles using Magnolia champaca plant floral extract for the first time. Computational analysis showed the role of GS-CuO nanoparticles on cardiac enzymes ACE2 and SOD1 functional expression through hydrogen bond interaction with amino acid residues. Method: The synthesized GS-CuO nanoparticles were characterized by various techniques like X-Ray Diffraction, UVVis Spectrophotometer, Fourier Transform Infrared Spectroscopy, Scanning Electron Microscopy and Transmission Electron Microscopy. Results: Nanoparticles demonstrate the presence of spherical shape and size 20 nm. The particles have many active sites and as compared to the bulk materials, and thus, the computational analysis was conducted against angiotensin-converting enzyme and superoxide dismutase to visualize the cardioprotective effects. Conclusion: The in-silico approach established valuable information on cardioprotective effects of green synthesized oxide nanoparticles using Magnolia champaca.

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Aqueous Extract of Saraca indica Leaves in the Synthesis of Copper Oxide Nanoparticles: Finding a Way towards Going Green

Journal of Nanotechnology ◽

10.1155/2017/7502610 ◽

2017 ◽

Vol 2017 ◽

pp. 1-6 ◽

Cited By ~ 13

Author(s):

Kollur Shiva Prasad ◽

Alakananda Patra ◽

Govindaraju Shruthi ◽

Shivamallu Chandan

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Copper Oxide ◽

Aqueous Extract ◽

Copper Oxide Nanoparticles ◽

Cuo Nanoparticles ◽

Oxide Nanoparticles ◽

X Ray ◽

Transmission Electron ◽

Varied Size

The present study is mainly aimed at the synthesis of copper oxide nanoparticles of varied size by green synthetic approach. The structural and morphological behavior of as-synthesized CuO nanoparticles were investigated using ultraviolet-visible spectral studies (UV-Vis), Fourier transform-Infrared spectroscopy (FT-IR), X-ray diffraction (XRD), energy dispersive X-ray spectroscopy (EDX), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and high-resolution transmission electron microscopy (HRTEM). The reduction of copper ions using aqueous extract of S. indica leaves produces nanoparticles of varied size and morphology. The images from SEM investigation revealed that the particles are spherical in shape with average diameter of 40–70 nm. TEM and HRTEM images clearly indicate the crystallinity and spherical nature of as-synthesized CuO nanoparticles with interplanar distance between two neighboring lattice fringes of 0.315 nm.

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STUDY OF STRUCTURAL AND DIELECTRIC PROPERTIES OF COPPER OXIDE NANOPARTICLES PREPARED BY WET CHEMICAL PRECIPITATION METHOD

International Journal of Nanoscience ◽

10.1142/s0219581x13500361 ◽

2013 ◽

Vol 12 (05) ◽

pp. 1350036 ◽

Cited By ~ 5

Author(s):

KIBRIYA SIDDIQUE ◽

BHABESH KUMAR NATH ◽

SANJIB KARMAKAR

Keyword(s):

Electron Microscopy ◽

Dielectric Properties ◽

Copper Oxide ◽

Chemical Precipitation ◽

Cuo Nanoparticles ◽

Chemical Precipitation Method ◽

Precipitation Method ◽

X Ray ◽

Wet Chemical ◽

Wet Chemical Precipitation

We report the synthesis of copper oxide ( CuO ) nanoparticles prepared by wet chemical precipitation method. The structural and dielectric properties are studied by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and complex impedance spectroscopy as a function of frequencies from 40 KHz to 100 KHz in the range of temperatures (308–393K). Maximum value of dielectric constants are found to be in the order of 106 which increases with increase in temperatures. From XRD data it is found that the particle size increases with increase in calcination temperatures. SEM with energy dispersive X-ray fluorescence spectrometer (EDX) results show that only CuO is present in the prepared sample. The selected area electron diffraction (SAED) pattern by TEM shows that uniform size distributions of CuO nanoparticles are present in the sample.

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Surface reconstructions of (001) cleaved GdBa2Cu3O7-δ

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010012093x ◽

1992 ◽

Vol 50 (1) ◽

pp. 106-107

Author(s):

H.W. Zandbergen ◽

M.R. McCartney

Keyword(s):

Electron Microscopy ◽

Copper Oxide ◽

Grain Boundaries ◽

Atomic Structure ◽

Oxygen Deficiency ◽

Surface Layers ◽

Layer Sequence ◽

Surface Reconstructions ◽

Good Agreement

Very few electron microscopy papers have been published on the atomic structure of the copper oxide based superconductor surfaces. Zandbergen et al. have reported that the surface of YBa2Cu3O7-δ was such that the terminating layer sequence is bulk-Y-CuO2-BaO-CuO-BaO, whereas the interruption at the grain boundaries is bulk-Y-CuO2-BaO-CuO. Bursill et al. reported that HREM images of the termination at the surface are in good agreement with calculated images with the same layer sequence as observed by Zandbergen et al. but with some oxygen deficiency in the two surface layers. In both studies only one or a few surfaces were studied.

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Imaging nanostructures on ablated kapton polymide

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100150198 ◽

1993 ◽

Vol 51 ◽

pp. 872-873

Author(s):

Daniel L. Callahan ◽

H. M. Phillips ◽

R. Sauerbrey

Keyword(s):

Electron Microscopy ◽

Polymer Surface ◽

Grating Period ◽

Laser Wavelength ◽

Integrated Devices ◽

Transmission Electron ◽

Excimer Laser Irradiation ◽

Produce Line ◽

Line Patterns ◽

Anisotropic Conduction

Excimer laser irradiation has been used to interferometrically ablate submicron line patterns on to Kapton polyimide. Such patterned material may exhibit highly anisotropic conduction as was predicted from previous studies showing enhanced conductivity from uniformly ablated material. We are currently exploiting this phenomenon to create integrated devices using conventional polymers as both dielectrics and conductors. Extensive scanning electron microscopy (SEM) and limited transmission electron microscopy (TEM) have been conducted in order to characterize the morphology of such patterned nanostructures as a function of processing conditions.The ablation technique employed produces an interference pattern on the polymer surface of period equal to half that of a diffraction grating period, independent of the laser wavelength. In these experiments, a 328 nm grating has been used to produce line patterns of 164 nm line-spacings as shown in Figures 1 and 2. A 200 Å Au coating has been used to both prevent charging and, perhaps more importantly, enhance contrast.

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Imaging of polymer single crystals in low-voltage, high-resolution scanning electron microscopy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100178665 ◽

1990 ◽

Vol 48 (4) ◽

pp. 1106-1107

Author(s):

W.W. Adams ◽

G. Price ◽

A. Krause

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

High Resolution ◽

Single Crystals ◽

Low Voltage ◽

Polymer Surface ◽

Beam Current ◽

Image Features ◽

First Results ◽

Scanning Electron

It has been shown that there are numerous advantages in imaging both coated and uncoated polymers in scanning electron microscopy (SEM) at low voltages (LV) from 0.5 to 2.0 keV compared to imaging at conventional voltages of 10 to 20 keV. The disadvantages of LVSEM of degraded resolution and decreased beam current have been overcome with the new generation of field emission gun SEMs. In imaging metal coated polymers in LVSEM beam damage is reduced, contrast is improved, and charging from irregularly shaped features (which may be unevenly coated) is reduced or eliminated. Imaging uncoated polymers in LVSEM allows direct observation of the surface with little or no charging and with no alterations of surface features from the metal coating process required for higher voltage imaging. This is particularly important for high resolution (HR) studies of polymers where it is desired to image features 1 to 10 nm in size. Metal sputter coating techniques produce a 10 - 20 nm film that has its own texture which can obscure topographical features of the original polymer surface. In examining thin, uncoated insulating samples on a conducting substrate at low voltages the effect of sample-beam interactions on image formation and resolution will differ significantly from the effect at higher accelerating voltages. We discuss here sample-beam interactions in single crystals on conducting substrates at low voltages and also present the first results on HRSEM of single crystal morphologies which show some of these effects.

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