scholarly journals Specific Protein Determinations Using Immunoelectrophoresis

1973 ◽  
Vol 10 (1-6) ◽  
pp. 4-13 ◽  
Author(s):  
Margaret J. Driscoll
Keyword(s):  
Sex Hormones ◽  
Diagnostic Tool ◽  
Serum Proteins ◽  
Specific Protein ◽  
Control Population ◽  
Reference Ranges ◽  
Specific Serum ◽  
Hospital Patients ◽  
Healthy Persons

The Laurell ‘rocket’ technique of immunoelectrophoresis has been used to quantitate seven specific serum proteins in a control population of SO healthy persons and in 100 hospital patients. Reference ranges have been established for each protein from data obtained from the control population. For many of the proteins variations due to age and/or sex were observed. Sex hormones, probably oestrogens, were also observed to cause variations in some of the protein concentrations. Data obtained from the hospital patients indicated that further work should prove profitable and could enable specific protein determinations to be useful, both as a diagnostic tool and in following the course of a disease.

scholarly journals The Protein Corona of PEGylated PGMA-Based Nanoparticles is Preferentially Enriched with Specific Serum Proteins of Varied Biological Function

Langmuir ◽  
2017 ◽  
Vol 33 (45) ◽  
pp. 12926-12933 ◽  
Author(s):  
Priya S. R. Naidu ◽  
Marck Norret ◽  
Nicole M. Smith ◽  
Sarah A. Dunlop ◽  
Nicolas L. Taylor ◽  
...  
Keyword(s):  
Biological Function ◽  
Serum Proteins ◽  
Protein Corona ◽  
Specific Serum

Optimizing Nephelometric Measurement of Specific Serum Proteins: Evaluation of Three Diluents

1974 ◽  
Vol 20 (12) ◽  
pp. 1548-1552 ◽  
Author(s):  
Lawrence M Killingsworth ◽  
Gregory J Buffone ◽  
Meena B Sonawane ◽  
Glennie C Lunsford
Keyword(s):  
Steady State ◽  
Continuous Flow ◽  
Reaction Rate ◽  
Serum Proteins ◽  
Physiological Saline ◽  
Rate Analysis ◽  
Specific Serum ◽  
State Approximation ◽  
Α2 Macroglobulin ◽  
Steady State Approximation

Abstract Three diluents were studied, to determine which is the best for the automated immunochemical measurement of specific serum proteins. Nine serum proteins (orosomucoid, α1-antitrypsin, α2-macroglobulin, haptoglobin, transferrin, C3, IgG, IgA, and IgM) were measured in physiological saline (9 g NaCI/liter), tris(hydroxymethyl)aminomethane buffer (0.01 mol/liter; pH 7.4), and physiological saline containing polyethylene glycol ("PEG 6000," 40 g/liter). Criteria were: reaction rate, analysis rate, carryover between samples, steady-state approximation, precision, and correlation with other methods. Saline containing polyethylene giycol is the best of the three diluents for use in continuous-flow nephelometric analysis of serum proteins.

The Transport of Thyroid Hormones

1983 ◽  
Vol 65 (4) ◽  
pp. 337-342 ◽  
Author(s):  
R. Hoffenberg ◽  
D. B. Ramsden
Keyword(s):  
Thyroid Hormones ◽  
Binding Proteins ◽  
Serum Proteins ◽  
High Capacity ◽  
Free Fraction ◽  
Total Serum ◽  
Free T4 ◽  
Specific Serum ◽  
Target Organs ◽  
Serum T4

1. Hormones have to be transported from their sites of synthesis to their target organs. For lipophilic hormones, such as steroids and thyroid hormones, transport is accomplished by binding to specific serum proteins, in the case of thyroxine (T4) and tri-iodothyronine (T3) to thyroxine-binding globulin (TBG) and prealbumin (PA). Normally about 70% of circulating T4 and 75–80% of T3 is bound to TBG, about 20% of T4 and 10% of T3 to PA and 10–15% of each to albumin, which has a low affinity but high capacity for both hormones [1, 2]. Apart from facilitating transport, binding to serum protein prevents excessive loss of hormone into the urine by glomerular filtration or flooding into cells, and may provide a readily available reservoir in times of need. The union between binding proteins and their ligands is reversible, so that a small proportion of hormone is non-protein-bound or ‘free’, in equilibrium with that which is protein-bound. For T4 this free fraction is normally 0.02-0.04% of the total serum T4 concentration, for T3 about 0.3% [3, 4]. 2. The major binding proteins of T4 and T3 will briefly be described and the nature of free T4 and T3 considered.

scholarly journals Use of procalcitonin and C-reactive protein as predictors and diagnostic tool of acute appendicitis

2020 ◽  
Vol 3 (2) ◽  
pp. 163-170
Author(s):  
Kafiah Rahed ◽  
Tariq Midhat ◽  
Noor Raheef
Keyword(s):  
Acute Appendicitis ◽  
Diagnostic Tool ◽  
Strong Positive Correlation ◽  
Age Group ◽  
Living Situation ◽  
Positive Correlation ◽  
Number Of Patients ◽  
Significant Difference ◽  
Group 15 ◽  
Healthy Persons

The aim of the study was to evaluate the role of PCT and CRP in patients with acute appendicitis. The study was conducted in Kirkuk city for the period from January, 2018 to April, 2018 on 50 patients with acute appendicitis with age group 15-54 years. Based on the clinical signs of patients, diagnostic tests and sonar rays, the number of patients with acute appendicitis was assigned to the present study. The study also included 40 healthy persons as control group . The study included the collection of 3 ml of venous blood for identification and measurement of PCT by using ELISA technique and CRP test by using Commercial manual kits. The study also included taking of full information from cases like living situation, age . The study indicated that the maximum mean of PCT was observed in patients with acute appendicitis as compared with healthy persons (17.31±0.51 versus 6.22±0.34ng/ml) with highly significant difference between the two groups. The maximum rate of CRP +ve was observed in patients with acute appendicitis as compared with healthy persons (84% versus 10% ng/ml) with highly significant difference between the two groups. The study found that means of WBCs count and neutrophils count were significantly higher in patients with acute appendicitis as compared with healthy persons. The study showed that there was positive correlation of PCT and CRP with each of WBCs and neutrophil counts and a strong positive correlation of PCT with CRP in patients with acute appendicitis. The highest rate of patients with acute appendicitis (40%) was in the age group 15-24 years followed by the age group 25-34 years while the lowest rate was in the age group 45-54 years. It was concluded that PCT and CRP considered as in important non-invasive diagnostic tool of acute bacterial appendicitis

151 PROTEOMICS ANALYSIS OF PREGNANCY-SPECIFIC SERUM PROTEINS IN BOVINE

2006 ◽  
Vol 18 (2) ◽  
pp. 183 ◽  
Author(s):  
D. I. Jin ◽  
H. R. Lee ◽  
H. R. Kim ◽  
H. J. Lee ◽  
J. T. Yoon ◽  
...  
Keyword(s):  
Heavy Chain ◽  
Early Pregnancy ◽  
Serum Proteins ◽  
Optical Resolution ◽  
Variable Region ◽  
Serum Samples ◽  
Proteomics Analysis ◽  
Specific Serum ◽  
Specific Proteins ◽  
Immunoglobulin Gamma

To identify early pregnancy-specific serum proteins in bovine, we performed proteomics analysis using blood serum samples of pregnant and non-pregnant Holstein dairy cattle Days 21 and 35 after AI. A total of eight pregnant and eight non-pregnant cattle were used for collection of the blood samples. The global proteomics approach was exploited by the use of 2-D gel electrophoresis and mass spectrometry to sort out pregnancy-specific proteins. Serum proteins within isoelectric point ranges of 4.0 to 7.0, 6.0 to 9.0, and 5.5 to 6.7 were analyzed separately by 2-D electrophoresis with three replications of each sample. The stained gels were scanned and calibrated at an optical resolution of 63.5 �m/pixel using a GS-710 imaging densitometer (Bio-Rad Laboratories, Philadelphia, PA, USA). A total of approximately 1200 spots were detected in 2-D gels stained with Coomassie-blue. In the comparison of serum samples from pregnant and non-pregnant cattle, nine pregnancy-specific spots were detected unanimously in Day 21 and Day 35 serum samples. Pregnancy-specific proteins were identified as transferrin, albumin, IgG2a heavy chain constant region, and immunoglobulin gamma heavy chain variable region by means of MALDI-TOF-MS (PerSeptive Biosystems, Framingham, MA, USA). Even though the identified spots were abundant serum proteins, their molecular weights and pI values were different from those of the main serum proteins. Most proteins identified in this analysis appeared to be related with pregnancy-specific subunits or fragments of transferrin, albumin, and IgG. One of the pregnancy-specific proteins, transferrin, is known to be related to iron transport during pregnancy. Western blot analysis using polyclonal anti-transferrin antibody revealed specific transferrin expression in the serum samples from the pregnant cattle but no detectable expression in the serum samples from the non-pregnant cattle. Our results revealed composite profiles of key proteins involved in early pregnancy and suggest the potential use of identified proteins to detect early pregnancy in bovine.

Definition and quantification of six immune- and neuroregulatory serum proteins in healthy and demented elderly

2019 ◽  
Vol 9 (4) ◽  
pp. 193-203 ◽  
Author(s):  
Carola G Schipke ◽  
Oliver Günter ◽  
Christina Weinert ◽  
Patrick Scotton ◽  
Jörg-Peter Sigle ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Blood Serum ◽  
Diagnostic Tool ◽  
Serum Proteins ◽  
Dementia Care ◽  
Healthy Controls ◽  
Analytic Procedure ◽  
The Future ◽  
Proof Of Principle

Aim: Blood-based biomarkers related to immune- and neuroregulatory processes may be indicative of dementia but lack standardization and proof-of-principle studies. Materials & methods: The blood serum collection protocol as well as the analytic procedure to quantify the markers BDNF, IGF-1, VEGF, TGF-β 1, MCP-1 and IL-18 in blood serum were standardized and their concentrations were compared between groups of 81 Alzheimer’s disease patients and 79 healthy controls. Results: Applying standardized methods, results for the quantification of the six markers in blood serum are stable and their concentrations significantly differ for all analytes except VEGF between patients diagnosed with Alzheimer’s disease and healthy controls. Conclusion: Analyzing a panel of six markers in blood serum under standardized conditions may serve as a diagnostic tool in primary dementia care in the future.

Serum proteins profile as an indicator of malignancy: multivariate logistic regression and ROC analyses

2005 ◽  
Vol 43 (9) ◽  
Author(s):  
Mehdi Rasouli ◽  
Ali Okhovatian ◽  
Atena Enderami
Keyword(s):  
Logistic Regression ◽  
Total Protein ◽  
Serum Proteins ◽  
Protein Pattern ◽  
Electrophoretic Pattern ◽  
Specific Protein ◽  
Multivariate Logistic Regression ◽  
Different Types ◽  
Electrophoretic Data ◽  
Spss Software

AbstractThe electrophoretic pattern of serum proteins of 85 patients carrying different types of neoplasia and 85 matched healthy adults were comparatively studied by agarose gel electrophoresis, to find out if there is a specific protein pattern common to different types of cancer. Each protein fraction was analyzed quantitatively by densitometry. Multivariate logistic regression and receiver operating characteristic (ROC) analyses were performed using SPSS software. When total protein and albumin were measured by colorimetric methods, cancer patients, compared to controls, had a decreased concentration of total protein (66.0±11.5g/L vs. 76.4±6.8g/L, p≤0.0001) and of albumin (39.0±8.1g/L vs. 46.0±4.3g/L, p≤0.0001). The electrophoretic data of serum proteins showed that the ratio of albumin to globulin (0.92±0.30 vs. 1.21±0.16, p≤0.0001), percent of the fractions albumin (46.7±8.5% vs. 54.4±3.5%, p≤0.0001) and β-globulin (11.6±4.4% vs. 13.0±1.9%, p≤0.001) were decreased and α

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