Interassay Differences in Growth Hormone Measurement in Acromegaly

1997 ◽  
Vol 34 (2) ◽  
pp. 156-159 ◽  
Author(s):  
J H Barth ◽  
J H Smith ◽  
P Clarkson
Keyword(s):  
Growth Hormone ◽  
Mean Value ◽  
Consistent Difference ◽  
Plasma Samples ◽  
Plasma Growth Hormone ◽  
Gh Isoforms ◽  
The Mean ◽  
Good Agreement

We measured plasma growth hormone concentrations by three different two-site immunometric assays (Pharmacia hGH RIA, IDS Gamma-BCT and Delfia 22 kDa hGH) to determine whether there are GH isoforms secreted by acromegalic patients that are under-recognized by some assays. There was a fairly good agreement between assays with the IDS Gamma-BCT and Delfia 22 kDa assays giving lower results than the Pharmacia IRMA. GH was measured on stored plasma samples from 24 patients with proven acromegaly. There was a consistent difference between the three assays of approximately 20% of the mean value for each patient.

ENZYMATISCH-FLUOROMETRISCHE BESTIMMUNG DER CORTISOLBINDUNGSKAPAZITÄT DES PLASMA

1967 ◽  
Vol 56 (1) ◽  
pp. 99-106 ◽  
Author(s):  
K. Leybold ◽  
J. Rieper ◽  
L. Weissbecker
Keyword(s):  
Binding Capacity ◽  
Liver Microsomes ◽  
Mean Value ◽  
Female Rats ◽  
Plasma Samples ◽  
Simple Method ◽  
Adult Men ◽  
The Mean ◽  
Precision And Accuracy

ABSTRACT A simple method for the determination of cortisol-binding capacity is described. For saturation of the cortisol-binding proteins, plasma samples are incubated with an excess of cortisol. In the next step NADPH and liver microsomes of female rats are added. The microsomal Δ4-3-ketosteroid hydrogenase only reduces non protein-bound cortisol to tetrahydrocortisol-5α. Then the steroids are extracted by dichloromethane, and after some purification steps analyzed by fluorometry. Tetrahydrocortisol gives practically no fluorescence. The cortisol determined by this method corresponds to protein-bound cortisol and indicates the extent of cortisolbinding capacity. Precision and accuracy of the method were found to be good. The values of cortisol-binding capacity obtained by our method are compared with the results of other authors. The mean value of adult men was 25.5 ± 3.4 μg/100 ml, that of pregnant women, mens IX-X, 42.3 ± 4.2 μg/100 ml.

Relation of concentration of growth hormone in blood plasma to growth rate and carcass characteristics in the pig

1975 ◽  
Vol 20 (1) ◽  
pp. 51-61 ◽  
Author(s):  
R. J. Chappel ◽  
A. C. Dunkin
Keyword(s):  
Growth Hormone ◽  
Growth Rate ◽  
Blood Plasma ◽  
Relative Growth Rate ◽  
Correlation Coefficients ◽  
Carcass Characteristics ◽  
Plasma Samples ◽  
Hormone Concentration ◽  
Plasma Growth Hormone ◽  
Relative Growth

SUMMARY1. Pig growth hormone (PGH) concentrations were measured in plasma samples from piglets of 3–4 to 7–8 weeks of age.2. Plasma PGH concentrations decreased significantly with age. No difference was observed between the hormone concentrations of gilts and barrows.3. Correlation coefficients were calculated between mean plasma growth hormone concentration over a 4-week period and several carcass characteristics and measures of growth rate. Plasma PGH over this period showed significant negative correlations with several measures of carcass backfat thickness at bacon weight. In barrows only, PGH concentrations showed a significant direct correlation with relative growth rate from 3 to 7 weeks.

scholarly journals An improved transmitter system to accurately measure wet-bulb temperature of air

2019 ◽  
Vol 52 (7-8) ◽  
pp. 1002-1007
Author(s):  
Liangwen Yan ◽  
Peng Yu ◽  
Sijung Hu ◽  
Qiu Gao ◽  
Wei Li ◽  
...  
Keyword(s):  
Real Time ◽  
Latent Heat ◽  
Measurement Accuracy ◽  
Cost Effective ◽  
Mean Value ◽  
Experimental Results ◽  
Iteration Algorithm ◽  
Good Consistency ◽  
The Mean ◽  
Good Agreement

A cost-effective measurement of wet-bulb temperature of air has great benefits to fulfill a growing demand of industry, cultivation agriculture, and medication. Applying an appropriate algorithm to wet-bulb temperature of air measurement can effectively improve the accuracy and speed of its measurement. The study aims to research how an improved transmitter system along with the latent heat–based iteration algorithm is used to precisely measure wet-bulb temperature of air. The work consists of (1) simulation of the iteration algorithm and (2) validation via experimental protocol. The simulation results through latent heat–based iteration algorithm were in good agreement ( R2≥ 0.99) with the reference. The performance of the improved wet-bulb temperature of air transmitter system was tested by a latent heat–based iteration algorithm experimental setup. The experimental results demonstrate that the improved wet-bulb temperature of air in a good consistency with commercial wet-bulb temperature of air in a range of temperature (15°C–34°C) and relative humidity (28.8%–76.2%). The Bland–Altman plot also shows that the mean value and the standard deviation of the differences between these two systems are 0.14°C and 0.29°C, respectively, which indicates that the improved wet-bulb temperature of air has a good agreement as well. Compared with the commercial wet-bulb temperature of air transmitter system, an advanced processor (STM32F103C8T6) and real-time operating system was applied in the improved wet-bulb temperature of air transmitter system. The experimental results show that its measurement accuracy is closer to the previous study. This study provides an alternative and cost-effective solution to accurately and real-time measure wet-bulb temperature of air.

scholarly journals Neutral Saturated Lapse Rate: An Experimental Check from CALJET-1998 and PACJET-2001

2009 ◽  
Vol 137 (12) ◽  
pp. 4382-4385 ◽  
Author(s):  
Renzo Richiardone ◽  
Massimiliano Manfrin
Keyword(s):  
High Resolution ◽  
Normal Distribution ◽  
Mean Value ◽  
Lapse Rate ◽  
Temperature Profiles ◽  
Experimental Check ◽  
Rate Distribution ◽  
The Mean ◽  
Lapse Rates ◽  
Good Agreement

Abstract The lapse rates of high-resolution temperature profiles during nearly neutral, saturated conditions are compared with the saturated adiabatic lapse rate and with that proposed by Richiardone and Giusti. A good agreement between the latter and the mean value of the observed lapse rate is found, whereas the saturated adiabatic lapse rate differs significantly, confirming experimentally that it is not completely correct to assess the moist neutrality from a comparison with the saturated adiabatic lapse rate. The lapse-rate distribution supports the hypothesis that the lapse-rate statistics is a local collection of saturated adiabatic lapse rates in a background normal distribution centered around the neutrality.

PLASMA INSULIN AND GROWTH HORMONE IN DAIRY COWS; DIURNAL VARIATION AND RELATION TO FOOD INTAKE AND PLASMA SUGAR AND ACETOACETATE LEVELS

1973 ◽  
Vol 73 (2) ◽  
pp. 289-303 ◽  
Author(s):  
Knut Hove ◽  
Anne Kristine Blom
Keyword(s):  
Growth Hormone ◽  
Food Intake ◽  
Diurnal Variation ◽  
Dairy Cows ◽  
Plasma Levels ◽  
Plasma Insulin ◽  
Diurnal Variations ◽  
Plasma Growth Hormone ◽  
The Mean ◽  
Different Levels

ABSTRACT Marked diurnal variations were found in plasma growth hormone (GH), insulin, acetoacetate (AcAc) and sugar in two herds (U and A) of dairy cows kept at two different levels of feeding. Seven animals from each herd were tested. The main diurnal variations were related to food intake, a significant increase in plasma insulin, and a significant decrease in plasma sugar being found. The acetoacetate level rose significantly during feeding in herd U (moderately underfed), while no significant increase was found in herd A (adequately fed). Plasma growth hormone was found to decrease (P < 0.01) only during feeding in herd U, while no change in the GH level could be detected in herd A. The mean level of GH in herd U was found to be twice the value found in herd A. There were no significant differences between the herds in plasma insulin and sugar. Significant differences in plasma levels of GH, insulin and sugar were found between animals when analysed within the herds. Variations in the levels of insulin and acetoacetate were very small during the night. This is contrary to GH, which shows the least variation during food intake. The correlation coefficient between the plasma components was low, although in many cases significant.

DIFFERENT EFFECTS OF EXOGENOUS CYCLIC ADENOSINE MONOPHOSPHATE AND ITS DIBUTYRYL DERIVATIVE ON PLASMA GROWTH HORMONE, GLUCOSE, INSULIN AND CORTISOL

1976 ◽  
Vol 83 (1) ◽  
pp. 15-25
Author(s):  
M. Vanderschueren-Lodeweyckx ◽  
W. Proesmans ◽  
E. Eggermont ◽  
R. Eeckels
Keyword(s):  
Growth Hormone ◽  
Hormone Secretion ◽  
Growth Hormone Secretion ◽  
Cyclic Adenosine Monophosphate ◽  
Adenosine Monophosphate ◽  
Normal Subjects ◽  
Immunoreactive Insulin ◽  
Plasma Growth Hormone ◽  
Hormone Levels ◽  
The Mean

ABSTRACT The effects of the infusion in four different dosages (0.001, 0.005, 0.02 and 0.2 mg/kg/min during 60 min) of cyclic 3′,5′-adenosine monophosphate and of its dibutyryl derivative on plasma growth hormone and on glucose, immunoreactive insulin and cortisol were studied in 38 normal subjects and in 10 patients with idiopathic hypopituitarism. In normal subjects cyclic 3′,5′-adenosine monophosphate provokes an increase in plasma growth hormone levels (only when a dosage of 0.2 mg/kg/min is used) without any changes in plasma glucose, insulin and cortisol. The maximal value of the means is observed 75 min after starting the infusion. Dibutyryl cyclic 3′,5′-adenosine monophosphate (0.2 and 0.02 mg/kg/min) provokes a dose-related rise in plasma growth hormone levels which is always preceded by hyperglycaemia and hyperinsulinaemia. The peak of the mean growth hormone levels occurs at 135 min after initiation of the infusion. In all but one hypopituitary patients the nucleotides do not promote growth hormone secretion. It is concluded that exogenous cyclic 3′,5′-adenosine monophosphate and its dibutyryl derivative may not be considered as analogous and that both compounds may contribute to study growth hormone release in normal subjects and in patients with growth abnormalities.

SOMATOMEDIN-LIKE ACTIVITY IN PLASMA AFTER FOETAL HYPOPHYSECTOMY OR NEPHRECTOMY AND IN EXPERIMENTAL INTRA-UTERINE GROWTH RETARDATION IN SHEEP

1979 ◽  
Vol 83 (1) ◽  
pp. 119-127 ◽  
Author(s):  
J. FALCONER ◽  
J. M. FORBES ◽  
I. C. HART ◽  
J. S. ROBINSON ◽  
G. D. THORBURN
Keyword(s):  
Growth Hormone ◽  
Gestational Age ◽  
Growth Retardation ◽  
Costal Cartilage ◽  
Maternal Plasma ◽  
Plasma Samples ◽  
Foetal Growth ◽  
Uterine Growth ◽  
The Mean ◽  
Intra Uterine Growth Retardation

SUMMARY Plasma samples from pregnant ewes and their foetuses during the last quarter of gestation were assayed for somatomedin-like activity (SLA) using the porcine costal cartilage assay. In maternal plasma, the mean potency (compared with pooled serum from six sheep) was 0·84 ± 0·05 (s.e.m.) units/ml (n = 15). Somatomedin-like activity in the plasma of five control foetuses (0·91 ± 0·1 units/ml) was similar to the maternal levels and did not change with gestational age. After foetal hypophysectomy the SLA in foetal plasma (0·37 ± 0·05 units/ ml, n = 4) was significantly less than in control animals. In two nephrectomized foetuses, the mean SLA in plasma (0·08 and 0·51 units/ml respectively) was less than in control animals. Retardation of intra-uterine foetal growth was induced by removal of endometrial caruncles before pregnancy in four sheep. The SLA in plasma from these foetuses was 0·38 ± 0·05 units/ml (P< 0·01 v. control animals). The results suggest that SLA in the foetus may be important in the regulation of foetal growth, but they also indicate that factors other than growth hormone may be important in the control of SLA in foetal plasma.

scholarly journals Discussion of Methods on Determining the Mean Matter Density of the Universe

1978 ◽  
Vol 79 ◽  
pp. 281-286
Keyword(s):  
Mass Density ◽  
Mean Value ◽  
Matter Density ◽  
Future Prospects ◽  
The Mean ◽  
Conventional Methods ◽  
The Universe ◽  
Good Agreement

Tammann: The question of the mean mass density in the Universe has been mentioned many times and the organisers thought it would be useful to look at future prospects for obtaining improved estimates of Ω. the methods may be split into two types. First, the conventional methods involve determining the luminosity density in the Universe and multiplying by an appropriate mass-to-luminosity ratio. It must be emphasised that the mass determinations are dynamical and that most of the mass is not visible. There is good agreement among independent workers about the luminosity density and this figure seems to be known within a factor of 2. the appropriate mean value of M/L is more controversial, values between 10 and 200 having been discussed in the preceding lectures. It is agreed, however, that if the mean value of M/L lies in this range, one cannot close the Universe. Perhaps one should be more cautious and say that even granted the uncertainties in the quantities involved, it is unlikely that the Universe is closed.

scholarly journals Giganto-acromegaly with Hypogonadotrophic Hypogonadism

1970 ◽  
Vol 10 (1) ◽  
pp. 22-24
Author(s):  
Mirza Azizul Hoque ◽  
Md Bakhtiar Azam ◽  
Md Golam Kibria Khan ◽  
Md Azharul Hoque ◽  
Quazi Deen Mohammad
Keyword(s):  
Growth Hormone ◽  
Pituitary Gland ◽  
Mean Value ◽  
Growth Plates ◽  
Gh Secretion ◽  
Hypogonadotrophic Hypogonadism ◽  
Resulting Condition ◽  
The Mean ◽  
Standard Deviations

Gigantism came from Greek word ‘Giant'. Pathologically, this condition results from the actions of excessive growth hormone (GH) secretion from the pituitary gland during childhood and adolescent before the closure of epiphyseal growth plates. When the height of an individual is several standard deviations above the mean value for the same age, sex, and ethnicity, the condition is known as gigantism. Pituitary gigantism is extremely rare. If hypersecretion of growth hormone occurs after closure of epiphyseal growth plates the resulting condition is acromegaly. Most patients with gigantism also have features of acromegaly.   doi:10.3329/jom.v10i1.1999 J Medicine 2009; 10: 22-24

GROWTH HORMONE RELATED TO INSULIN AND SUGAR IN NOCTURNAL BLOOD PLASMA OF LACTATING COWS

1976 ◽  
Vol 82 (4) ◽  
pp. 767-773 ◽  
Author(s):  
K. Halse ◽  
Anne Kristine Blom ◽  
K. Hove
Keyword(s):  
Growth Hormone ◽  
Blood Plasma ◽  
Immunoreactive Insulin ◽  
Plasma Samples ◽  
Plasma Growth Hormone ◽  
Lactating Cows ◽  
High Insulin ◽  
Plasma Immunoreactive Insulin ◽  
Correlation Tests ◽  
Lactation Cycle

ABSTRACT Nocturnal blood plasma samples from about 190 indoor-fed cows at varying stages of the lactation cycle showed wide variations in the concentrations of sugar (43–88 mg/100 ml) and acetoacetate (0.12–8.7 mg/100 ml). Plasma immunoreactive insulin decreased and acetoacetate increased with decreasing sugar. Plasma growth hormone (GH) was significantly correlated to insulin (r = −0.24, P < 0.001) and to sugar (r = −0.15, P < 0.05), but not to acetoacetate (r = +0.13). Since the plasma level of GH in bovines is known to vary widely from hour to hour, individual cows are poorly represented by single determinations of the hormone. However, in accordance with the results from the correlation tests, different GH averages, about 11 and 9 ng/ml, were found for groups of animals with plasma sugar respectively below and above 70 mg/100 ml. By subgrouping according to insulin levels within sugar ranges, categories could be singled out with GH averages as different as 12.7 ng/ml (low sugar - low insulin) and 8.3 ng/ml (high sugar - high insulin). The findings are consistent with the view that GH is involved in the regulation of energy metabolism in bovines. But no support has been provided for the hypothesis of GH hypersecretion as a cause of ketosis in cows.

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