scholarly journals A Practical Technique to Postfix Nanogold-immunolabeled Specimens with Osmium and to Embed Them in Epon for Electron Microscopy

2000 ◽  
Vol 48 (4) ◽  
pp. 493-498 ◽  
Author(s):  
Hajime Sawada ◽  
Michiyo Esaki
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Light And Electron Microscopy ◽  
Chloroauric Acid ◽  
Plastic Film ◽  
Silver Acetate ◽  
Silver Enhancement ◽  
Transmission Electron ◽  
Gold Probe ◽  
Silver Shell

Nanogold is a tiny gold probe, freely diffusible in cells and tissues, and is suitable for pre-embedding immunohistochemistry. However, it is necessary to develop Nanogold to a larger size so that it can be observed by conventional transmission electron microscopy. Silver enhancement is usually used for visualizing Nanogold, but the silver shell produced is unstable in OsO4 and often becomes invisible after OsO4 postfixation, which is necessary for good visualization of ultrastructure. We used silver enhancement with silver acetate, followed by gold toning with chloroauric acid, to replace the silver shell with a more stable gold in order to observe Nanogold after osmium fixation and Epon embedding. This technique is applicable to various intra- and extracellular antigens. For correlative observation of immunolabled specimens by light and electron microscopy, specimens adhered to slideglasses were embedded in Epon under nonadhesive plastic film. By heating the Epon sheets after polymerization, these supports were removed without difficulty and provided easy correlative observation.

Scale structure and taxonomy of some species of Raphidocystis, Raphidiophrys, and Pompholyxophrys (Heliozoea) including descriptions of six new taxa

1985 ◽  
Vol 63 (8) ◽  
pp. 1944-1961 ◽  
Author(s):  
K. H. Nicholls ◽  
M. Dürrschmidt
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
New Zealand ◽  
Light Microscopy ◽  
Light And Electron Microscopy ◽  
Scale Structure ◽  
Taxonomic Descriptions ◽  
Transmission Electron ◽  
New Information ◽  
Freshwater Habitats

Sixteen taxa of the genera Raphidocystis, Raphidiophrys, and Pompholyxophrys from freshwater habitats in Canada, Chile, and New Zealand were studied by light and electron microscopy. Six taxa are described as new: Raphidocystis glabra, Raphidiophrys minuta, Raphidiophrys orbicularis ssp. orbicularis, R. orbicularis ssp. ovalis, Pompholyxophrys stellata, and P. ossea. New information on scale structure and arrangement based on scanning and transmission electron microscopy amplifies the taxonomic descriptions of Raphidiophrys ambigua, R. pallida, R. elegans, R. intermedia, R. marginata, R. symmetrica, Pompholyxophrys punicea, P. exigua, and P. ovuligera, which were previously imperfectly known by light microscopy only.

A Simple Method to Self-Assemble Two-dimensional and Three-dimensional Superlattices of Gold Nanoparticles

2007 ◽  
Vol 1056 ◽  
Author(s):  
Tao Fu ◽  
Hai-Yan Qin ◽  
Wen-Jiang Li ◽  
Sailing He
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Gold Nanoparticles ◽  
Particle Dispersion ◽  
Diameter Distribution ◽  
Chloroauric Acid ◽  
Two Dimensional ◽  
Visible Absorption ◽  
Simple Method ◽  
Transmission Electron

ABSTRACTCitrate-capped gold nanoparticles with narrow diameter distribution were prepared by the reduction of chloroauric acid with borohydride. After the gold nanoparticles were transferred to toluene solution through ligand conformation change, close-packed two-dimensional nanocrystal lattices were obtained on transmission electron microscopy (TEM) copper grids by evaporating approximately 10 μL of concentrated particle dispersion. The formation of the thiolate gold nanoparticles was investigated by UV-visible absorption spectroscopy and FTIR spectra. The 2D Fourier transform power spectra of the monolayer confirmed the supperlattices' hexagonal symmetry. Furthermore, long-range-ordered bilayer superlattices, of which the nanoparticles in the top layer piled on the ones in the bottom layer, could also be observed by transmission electron microscopy.

The proboscis ciliation of the echiuran Bonellia viridis

1979 ◽  
Vol 59 (2) ◽  
pp. 377-384 ◽  
Author(s):  
V. Jaccarini ◽  
P. J. Schembri
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Quantitative Analysis ◽  
Light And Electron Microscopy ◽  
Neck Region ◽  
Dorsal Surface ◽  
Food Particle ◽  
Particle Uptake ◽  
Transmission Electron ◽  
Definite Pattern

Special club-shaped cilia with an expanded tip are seen by both light and electron microscopy on the proboscis of Bonellia viridis in addition to the usual filamentous cilia. Using transmission electron microscopy the club tip is seen to consist of a distally curled axoneme enclosed within a sac-like expansion of the ciliary membrane. The axoneme may describe up to two complete loops inside the membrane sac or may occasionally follow an S-shaped course.A quantitative analysis of the distribution on the proboscis of both filamentous and club cilia is made. No discrete ciliary tracts are present. However, there is a definite pattern of ciliary density distribution. The cilia are densest on both the dorsal and ventral surfaces of the distal fringe of the proboscis. The dorsal fringe cilia are concerned with locomotion and the ventral cilia with food particle uptake. The club cilia are confined to the terminal lobes and neck region of the proboscis. The dorsal surface of the proboscis posterior to the fringe is virtually non-ciliated. The functional significance of the club cilia is discussed.

scholarly journals Correlative Light and Transmission Electron Microscopy Showed Details of Mitophagy by Mitochondria Quality Control in Propionic Acid Treated SH-SY5Y Cell

Materials ◽  
2020 ◽  
Vol 13 (19) ◽  
pp. 4336
Author(s):  
Minkyo Jung ◽  
Hyosun Choi ◽  
Jaekwang Kim ◽  
Ji Young Mun
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Propionic Acid ◽  
Hippocampal Neurons ◽  
Acid Treatment ◽  
Light And Electron Microscopy ◽  
Quality Monitoring ◽  
Selective Autophagy ◽  
Transmission Electron ◽  
The Brain

Propionic acid is a metabolite of the microbiome and can be transported to the brain. Previous data show that propionic acid changes mitochondrial biogenesis in SH-SY5Y cells and induces abnormal autophagy in primary hippocampal neurons. Maintaining mitochondrial function is key to homeostasis in neuronal cells, and mitophagy is the selective autophagy involved in regulating mitochondrial quality. Monitoring mitophagy though light microscopy or conventional transmission electron microscopy separately is insufficient because phases of mitophagy, including autophagosome and autolysosome in nano-resolution, are critical for studies of function. Therefore, we used correlative light and electron microscopy to investigate mitochondrial quality in SH-SY5Y cells after propionic acid treatment to use the advantages of both techniques. We showed, with this approach, that propionic acid induces mitophagy associated with mitochondrial quality.

scholarly journals Precision of fiducial marker alignment for correlative super‐resolution fluorescence and transmission electron microscopy

2021 ◽  
Vol 1 (1) ◽  
Author(s):  
Adeeba Fathima ◽  
César Augusto Quintana-Cataño ◽  
Christoph Heintze ◽  
Michael Schlierf
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Single Molecule ◽  
Fluorescent Proteins ◽  
Light And Electron Microscopy ◽  
Super Resolution ◽  
Specific Protein ◽  
Fiducial Markers ◽  
Transmission Electron ◽  
Microscopy Techniques

AbstractRecent advances in microscopy techniques enabled nanoscale discoveries in biology. In particular, electron microscopy reveals important cellular structures with nanometer resolution, yet it is hard, and sometimes impossible to resolve specific protein localizations. Super-resolution fluorescence microscopy techniques developed over the recent years allow for protein-specific localization with ~ 20 nm precision are overcoming this limitation, yet it remains challenging to place those in cells without a reference frame. Correlative light and electron microscopy (CLEM) approaches have been developed to place the fluorescence image in the context of a cellular structure. However, combining imaging methods such as super resolution microscopy and transmission electron microscopy necessitates a correlation using fiducial markers to locate the fluorescence on the structures visible in electron microscopy, with a measurable precision. Here, we investigated different fiducial markers for super-resolution CLEM (sCLEM) by evaluating their shape, intensity, stability and compatibility with photoactivatable fluorescent proteins as well as the electron density. We further carefully determined limitations of correlation accuracy. We found that spectrally-shifted FluoSpheres are well suited as fiducial markers for correlating single-molecule localization microscopy with transmission electron microscopy.

Morphological effects of Epiccocum nigrum and its antibiotic flavipin on Monilinia laxa

1995 ◽  
Vol 73 (3) ◽  
pp. 425-431 ◽  
Author(s):  
Carlos Madrigal ◽  
Paloma Melgarejo
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Light And Electron Microscopy ◽  
Lipid Bodies ◽  
Monilinia Laxa ◽  
Epicoccum Nigrum ◽  
Transmission Electron ◽  
Germ Tubes ◽  
Scanning Electron

The influence of an isolate of Epicoccum nigrum and one of its antibiotics, flavipin, on the spores, mycelium, and germ tubes of Monilinia laxa in culture was studied using light and electron microscopy. Epicoccum nigrum and flavipin induced the development of stromata in cultures. Abundant clusters of microconidia of M. laxa were produced on the induced stromata exposed to E. nigrum and flavipin. Deformation of hyphae and germ tubes such as swellings, coilings, and abnormal ramifications were also noticeable under light and scanning electron microscopy after treatment with E. nigrum or flavipin. Transmission electron microscopy revealed cytoplasmic coagulation of the cells and abundant vacuoles and lipid bodies associated with membranes. In some cases alteration and disorganization of membranes was also apparent. Key words: antagonism, antibiosis, flavipin, biological control.

Trophozoites of Pathogenic Naegleria: Scanning Electron Microscope Study

1975 ◽  
Vol 33 ◽  
pp. 652-653
Author(s):  
A. Julio Martinez ◽  
E. Clifford Nelson ◽  
Doris G. Fultz ◽  
Ragnit Geeraets
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Electron Microscope Study ◽  
Plastic Film ◽  
Scanning Electron Microscope Study ◽  
Transmission Electron ◽  
Scanning Electron

Scanning electron microscopy (SEM) can serve as a valuable supplement to transmission electron microscopy (TEM) in the study of pathogenic protozoa. Details of overall form and structure of surface and organelles which may have a role in pathogenicity may be revealed. TEM studies on Naegleria have contributed much to understanding the extraordinary virulence of this ameba, but some remaining questions may be resolved by SEM. This report describes a technique which has proven useful in preparing SEM specimens. Naegleria ameba trophozoites adhere strongly to the surface on which they are growing. In culture tubes, amebae will multiply on the wall. Naegleria tends to grow from the top of the fluid downward and may multiply until a solid monolayer develops. If a strip of plastic film is introduced, the growth on the strip can be observed by direct microscope viewing through the wall of the tube.

Sign in / Sign up

Export Citation Format

Share Document