Polyvinylchloride surface with enhanced cell/bacterial adhesion-resistant and antibacterial functions

2019 ◽  
Vol 33 (10) ◽  
pp. 1415-1426
Author(s):  
Rashed Almouse ◽  
Xin Wen ◽  
Sungsoo Na ◽  
Gregory Anderson ◽  
Dong Xie
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Bacterial Adhesion ◽  
Surface Coating ◽  
Antibacterial Properties ◽  
Fibroblast Cell ◽  
Mouse Fibroblast ◽  
Fibroblast Cells ◽  
Hydrophilic Polymer ◽  
Surface Adhesion

This study reports synthesis and attachment of a novel antibacterial and hydrophilic polymer onto a polyvinylchloride surface via a simple and mild surface coating technique. The compound 3,4-dichloro-5-hydroxy-2(5H)-furanone was derivatized and copolymerized with N-vinylpyrrolidone. The copolymer was then covalently coated onto polyvinylchloride surface. 3T3 mouse fibroblast cells and bacterium Pseudomonas aeruginosa were used to evaluate surface adhesion and antibacterial activity. Results showed that the polymer-modified polyvinylchloride surface not only exhibited significantly decreased 3T3 fibroblast cell adhesion with a 64–84% reduction but also demonstrated significantly decreased P. aeruginosa adhesion with a 65–84% reduction, as compared to unmodified polyvinylchloride. Furthermore, the modified polyvinylchloride surfaces exhibited significant antibacterial functions by inhibiting P. aeruginosa growth with a 58–80% reduction and killing bacteria, as compared to unmodified polyvinylchloride. These results demonstrate that covalent polymer attachment conferred cell/bacterial adhesion-resistant and antibacterial properties to the polyvinylchloride surface.

scholarly journals Antibacterial activity of Zataria multiflora essential oil and its main components against Pseudomonas aeruginosa

2017 ◽  
Vol 63 (3) ◽  
pp. 18-24 ◽  
Author(s):  
Mohaddese Mahboubi ◽  
Rezvan Heidarytabar ◽  
Elaheh Mahdizadeh
Keyword(s):  
Gas Chromatography ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Antibacterial Properties ◽  
Antibacterial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Zataria Multiflora ◽  
Main Components

Summary Introduction: In Iranian traditional medicine, Zataria multiflora Boiss (Lamiaceae family) is reputed due to its antiseptic effects. Objective: The purpose of this study was to evaluate the antibacterial and biofilm killing effects of Z. multiflora essential oil and main components against Pseudomonas aeruginosa. Methods: The main components of essential oil were identified by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The antibacterial properties of Z. multiflora oil and main components were determined by assessing the MIC and MBC values, and their inhibition percent of biofilm killing effects were determined by the evaluation of optical density. The role of each main component in these activities was determined according to the chemical profiles of essential oil. Results: Thymol (38.7%), carvacrol (30.6%), and p-cymene (8.3%) were main components of twenty five components of essential oil. Carvacrol had the higher role in antibacterial activity against P. aeruginosa, followed by thymol. P-cymene enhanced the antibacterial activities of thymol and carvacrol against P. aeruginosa. Carvacrol showed the weak role in biofilm killing effect. In spite of the low antibacterial activity of p-cymene against P. aeruginosa, it can enhance the antibacterial activity of thymol or carvacrol. Conclusion: Z. multiflora essential oil can be used for the management of P. aeruginosa infections. Determining the precise role of each components needs investigating in their behavior in different media.

scholarly journals Synthesis and Antibacterial Activity of Antibiotic-Functionalized Graphite Nanofibers

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Madeline Rotella ◽  
Alicia Briegel ◽  
John Hull ◽  
Anthony Lagalante ◽  
Robert Giuliano
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Surface Functionalization ◽  
Antibacterial Properties ◽  
Carboxyl Groups ◽  
Current Investigation ◽  
Graphite Nanofibers ◽  
Quinolone Antibiotics ◽  
Graphitic Carbon Nanofibers ◽  
Density Methods

Surface functionalization of nanomaterials is an area of current investigation that supports the development of new biomaterials for applications in biology and medicine. Herein we describe the synthesis, characterization, and antibacterial properties of the first examples of antibiotic-labeled graphitic carbon nanofibers (GCNFs) covalently functionalized with aminoglycoside and quinolone antibiotics. Ruthenium tetroxide oxidation of herringbone GCNFs gave higher amounts of surface carboxyl groups than previous methods. These carboxyl groups served as sites of attachment for antibiotics by acyl substitution. Bioassay of these novel, functionalized GCNFs using serial dilution and optical density methods demonstrated that antibiotic-labeled GCNFs possess significant antibacterial activity againstPseudomonas aeruginosa. The activity we observe for aminoglycoside-functionalized GCNFs suggests a membranolytic mechanism of action.

scholarly journals Green synthesis of non-cytotoxic silver nanoparticles using Solanum nigrum leaves extract with antibacterial properties

2021 ◽  
Vol 15 (3) ◽  
pp. 262-271
Author(s):  
Anushaa A ◽  
Pushpa Agrawal ◽  
Vijaya Kumar G ◽  
Thippareddy KS
Keyword(s):  
Silver Nanoparticles ◽  
Foodborne Pathogens ◽  
High Efficiency ◽  
Antibacterial Properties ◽  
Cost Effective ◽  
Fibroblast Cell ◽  
Mouse Fibroblast ◽  
Solanum Nigrum ◽  
Simple Method ◽  
Efficiency Cost

Green synthesizes of silver nanoparticles is a promising method in material science and nanotechnology today. Silver nanoparticles (AgNP) were synthesized using a high-efficiency, cost-effective green and simple method at room temperature using Solanum nigrum leaves extract. The biosynthesized AgNP was validated and classified using spectroscopy profiles from ultraviolet-visible spectroscopy, scanning electron microscope, Fourier-transform infrared spectroscopy and x-ray diffraction. FTIR spectra indicated the presence of biological molecules in AgNP synthesis and UV-visible spectra revealed a surface resonance peak of 420 nm corresponding to AgNP formation. The antimicrobial potential of these synthesized nanoparticles is evaluated against the bacteria Escherichia coli (MCC 2079) and Staphylococcus aureus (MCC 2408), both of which are important foodborne pathogens. Ciprofloxacin and Clotrimazole were used as positive controls antibiotic. The nanoparticles were shown to have strong antibacterial efficacy against the strains examined. Their antibacterial function allows them to be included in antimicrobial formulations. By using the MTT assay, the biocompatibility of Solanum nigrum silver nanoparticles were found non-toxic to mouse fibroblast cell lines (L929 is one of the first in continuous culture to be created. The L strain was generated from a male C3H mouse's usual subcutaneous areolar and adipose tissue.) at lower concentrations.

scholarly journals Physico-chemical characterization and antibacterial activity of different types of honey tested on strains isolated from hospitalized patients

2016 ◽  
Vol 60 (1) ◽  
pp. 5-18 ◽  
Author(s):  
Lia M. Junie ◽  
Mihaela L. Vică ◽  
Mirel Glevitzky ◽  
Horea V. Matei
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Bacterial Species ◽  
Antibacterial Properties ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Disk Diffusion Method ◽  
Physico Chemical

AbstractThe first aim of the study was to compare the antibacterial activity of several types of honey of different origins, against some bacterial resistant strains. The strains had been isolated from patients. The second aim was to discover the correlations between the antibacterial character of honey and the physico-chemical properties of the honey. Ten honey samples (polyfloral, linden, acacia, manna, and sunflower) from the centre of Romania were tested to determine their antibacterial properties against the following bacterial species: Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, Salmonella enterica serovar Typhimurium, Bacillus cereus, Bacillus subtilis, and Listeria monocytogenes. Bacterial cultures in nutrient broth and the culture medium Mueller-Hinton agar were used. The susceptibility to antibiotics was performed using the disk diffusion method. All honey samples showed antibacterial activity on the isolated bacterial strains, in particular polyfloral (inhibition zone 13-21 mm in diameter) - because it is the source of several plants, and manna (inhibition zone 13-19.5 mm in diameter), and sunflower (inhibition zone 14-18.5 mm in diameter). Pure honey has a significant antibacterial activity against some bacteria which are resistant to antibiotics. Bacterial strains differed in their sensitivity to honeys. Pseudomonas aeruginosa and Staphylococcus aureus were the most sensitive. The present study revealed that honey antibacterial activity depends on the origin of the honey. We also found that there was a significant correlation between antibacterial activity of honeys and the colour of the honey but not between acidity and pH. The statistical analysis showed that the honey type influences the antibacterial activity (diameter of the bacterial strains inhibition zones).

scholarly journals In Vitro Evaluation of Palestinian Propolis as a Natural Product with Antioxidant Properties and Antimicrobial Activity against Multidrug-Resistant Clinical Isolates

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Jamila Daraghmeh ◽  
Hamada Imtara
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Antibacterial Properties ◽  
Reducing Power ◽  
Multidrug Resistant ◽  
Clinical Isolates ◽  
Total Phenolic

The present work reveals, for the first time, the antioxidant and antibacterial properties of propolis samples collected from different regions of Palestine. The content on bioactive compounds has been estimated by total phenolic and flavone and flavonol content, while their antioxidant activity has been determined by radical scavenging methods of 1,2-diphenyl-1-picrylhydrazyl radical (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) radical (ABTS), and ferric reducing power assay (FRAP). The disc diffusion, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) methods were carried out to evaluate the antibacterial activity of Palestinian propolis against multidrug-resistant clinical isolates, including Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus faecalis. The results showed that the total phenolic content ranged from 9.62 mg to 124.94 mg gallic acid equivalent GAE/g of propolis, and the flavone and flavonol content ranged from 1.06 to 75.31 mg quercetin equivalent QE/g of propolis. The samples S6 from Al-Khalil presented the strongest radical scavenging activity toward DPPH, ABTS free radicals, and FRAP assay with IC50 values of 0.02, 0.03, and 0.05 mg/mL, respectively. The results of antibacterial activity indicated that the propolis samples inhibit the growth of Gram-positive strains better than Gram-negative ones. In addition, a strong correlation was observed between the pH, resin, balsam, total phenolic, flavones and flavonol, and total antioxidant capacity (TAC) from one side and the antibacterial activity of propolis samples except on Pseudomonas aeruginosa.

scholarly journals Antibacterial Effect of Stainless Steel Surfaces Treated with a Nanotechnological Coating Approved for Food Contact

2021 ◽  
Vol 9 (2) ◽  
pp. 248
Author(s):  
Alessandro Di Cerbo ◽  
Andrea Mescola ◽  
Giuseppe Rosace ◽  
Roberta Stocchi ◽  
Giacomo Rossi ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Bacterial Adhesion ◽  
Surface Coating ◽  
Bacterial Colonization ◽  
Bactericidal Effect ◽  
Stainless Steel Surface ◽  
Surface Adhesion ◽  
Bacterial Strains ◽  
Gram Positive Bacteria ◽  
Consequent Reduction

Stainless steel, widely present in the food industry, is frequently exposed to bacterial colonization with possible consequences on consumers’ health. 288 stainless steel disks with different roughness (0.25, 0.5 and 1 μm) were challenged with four Gram-negative (Escherichia coli ATCC 25922, Salmonella typhimurium ATCC 1402, Yersinia enterocolitica ATCC 9610 and Pseudomonas aeruginosa ATCC 27588) and four Gram-positive bacteria (Staphylococcus aureus ATCC 6538, Enterococcus faecalis ATCC 29212, Bacillus cereus ATCC 14579 and Listeria monocytogenes NCTT 10888) and underwent three different sanitizing treatments (UVC, alcohol 70% v/v and Gold lotion). Moreover, the same procedure was carried out onto the same surfaces after a nanotechnological surface coating (nanoXHAM® D). A significant bactericidal effect was exerted by all of the sanitizing treatments against all bacterial strains regardless of roughness and surface coating. The nanoXHAM® D coating itself induced an overall bactericidal effect as well as in synergy with all sanitizing treatments regardless of roughness. Stainless steel surface roughness is poorly correlated with bacterial adhesion and only sanitizing treatments can exert significant bactericidal effects. Most of sanitizing treatments are toxic and corrosive causing the onset of crevices that are able to facilitate bacterial nesting and growth. This nanotechnological coating can reduce surface adhesion with consequent reduction of bacterial adhesion, nesting, and growth.

scholarly journals Ciprofloxacin-Releasing ROS-Sensitive Nanoparticles Composed of Poly(Ethylene Glycol)/Poly(D,L-lactide-co-glycolide) for Antibacterial Treatment

Materials ◽  
2021 ◽  
Vol 14 (15) ◽  
pp. 4125
Author(s):  
Jaeik Song ◽  
Min-Suk Kook ◽  
Byung-Hoon Kim ◽  
Young-IL Jeong ◽  
Kyung-Jin Oh
Keyword(s):  
Antibacterial Activity ◽  
Ethylene Glycol ◽  
Distribution Pattern ◽  
Inhibitory Effect ◽  
Mouse Fibroblast ◽  
Human Skin Fibroblast ◽  
Fibroblast Cells ◽  
Cell Viability Assay ◽  
Tract Infections

Since urinary tract infections (UTIs) are closely associated with oxidative stress, we developed ROS-sensitive nanoparticles for ciprofloxacin (CIP) delivery for inhibition of UTI. Poly(D,L-lactide-co-glycolide) (PLGA)- selenocystamine (PLGA-selenocystamine) conjugates were attached to methoxypoly(ethylene glycol) (PEG) tetraacid (TA) (TA-PEG) conjugates to produce a copolymer (abbreviated as LGseseTAPEG). Selenocystamine linkages were introduced between PLGA and TA to endow reactive oxygen species (ROS) sensitivity to nanoparticles. CIP-incorporated nanoparticles of LGseseTAPEG copolymer were fabricated by W/O/W/W emulsion method. CIP-incorporated nanoparticles responded to H2O2 and then their morphologies were disintegrated by incubation with H2O2. Furthermore, particle size distribution of nanoparticles was changed from mono-modal distribution pattern to multi-modal distribution pattern by addition of H2O2. CIP release from nanoparticles of LGseseTAPEG copolymer was faster in the presence of H2O2 than in the absence of it. In antibacterial study using Escherichia coli (E. coli), free CIP and free CIP plus empty nanoparticles showed dose-dependent inhibitory effect against growth of bacteria while CIP-incorporated nanoparticles have less antibacterial activity compared to free CIP. These results were due to that CIP-incorporated nanoparticles have sustained release properties. When free CIP or CIP-incorporated nanoparticles were introduced into dialysis membrane to mimic in vivo situation, CIP-incorporated nanoparticles showed superior antibacterial activity compared to free CIP. At cell viability assay, nanoparticles of LGseseTAPEG copolymer have no acute cytotoxicity against L929 mouse fibroblast cells and CCD986sk human skin fibroblast cells. We suggest LGseseTAPEG nanoparticles are a promising candidate for CIP delivery.

scholarly journals ANTIBACTERIAL ACTIVITY OF SOME NANO-ANTIMICROBIAL POLY(AMIDOAMINE) (PAMAM) DENDRIMERIC PRODUCTS

2021 ◽  
Vol 80 (2) ◽  
pp. 133-140
Author(s):  
Costin Ștefan Caracoti ◽  
Ștefania Mădălina Negrea ◽  
Carmen Ana Isabelle Florea
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Acinetobacter Baumannii ◽  
Bacterial Species ◽  
Antibacterial Properties ◽  
Percentage Reduction ◽  
Pharmacologically Active ◽  
Logarithmic Reduction ◽  
Active Substances

"Dendrimers are synthetic, branched macromolecules. Compared to linear polymers, dendrimers hold multiple advantages as potential carrier molecules: high capacity to incorporate pharmacologically active substances, chemical stability of micelles under physiological conditions, increased bioavailability of active substances covalently or noncovalently bound to dendrimers, due to increased dendrimers capacity to cross biological barriers. The carrier potential of dendrimers has been evaluated in combination with different antibiotics, as an alternative way to address the increasing resistance of Gram-positive and Gram-negative bacteria. This paper presents a study on the intrinsic antibacterial properties of the following poly(amidoamino) (PAMAM) ethylenediamine core dendrimers: PAMAM generation 3.5, PAMAM generation 4.0 and PAMAM-OH generation 4.0. The following bacterial strains were used: Staphylococcus aureus ATCC 29213, Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 15442, Enterococcus faecalis ATCC 51299 and a multi-drug resistant (MDR) Acinetobacter baumannii clinical isolate. The dendrimers’ concentrations used in the experiment were established within the range of admissibility obtained following cytotoxicity testing on human cell cultures. The dendrimers’ antibacterial activity varied depending on the concentration and the bacterial species. The antibacterial efficacy was quantified by calculating the percentage and logarithmic reduction of the number of viable microorganisms. The logarithmic reduction varied between 0.06 and 1.10, corresponding to percentage reductions between 13% and 92%. The strongest antibacterial activity of all dendrimers used in this study was registered for the D3.5 dendrimer (50 μg/mL) against Acinetobacter baumannii and Pseudomonas aeruginosa ATCC 15442 reference strain. For A. baumannii, a logarithmic reduction of bacterial activity of 1.10 log10 (percentage reduction in the number of CFU of 92%) was registered, while for P. aeruginosa ATCC 15442 the logarithmic reduction was 0.75 log10 (percentage reduction of 82%)."

Antibacterial Activity of Desert Truffles from Saudi Arabia Against Staphylococcus aureus and Pseudomonas aeruginosa

2017 ◽  
Vol 19 (2) ◽  
pp. 121-125 ◽  
Author(s):  
Domenico Schillaci ◽  
Maria Grazia Cusimano ◽  
Stella Maria Cascioferro ◽  
Vita Di Stefano ◽  
Vincenzo Arizza ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Saudi Arabia ◽  
Desert Truffles

Chemical Composition and Antibacterial Activity of Essential Oils of Three Medicinal Plants from Algerian Semi-Arid Climatic Zone

2018 ◽  
Vol 16 (S1) ◽  
pp. S155-S163 ◽  
Author(s):  
S. Mehalaine ◽  
O. Belfadel ◽  
T. Menasria ◽  
A. Messaili
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Rosmarinus Officinalis ◽  
Salvia Officinalis ◽  
Thymus Algeriensis

The present study was carried out to determine, for the first time, the chemical composition and antibacterial activity of essential oils derived from the aerial parts of three aromatic plants Thymus algeriensis Boiss & Reut, Rosmarinus officinalis L., and Salvia officinalis L. growing under semiarid conditions. The essential oils were chemically analyzed and identified by gas chromatography (GC) and GC/ mass spectrometry (GC/MS) and their antimicrobial activity was individually evaluated against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa using both agar disk diffusion and agar dilution methods. The major constituents of Thymus algeriensis essential oil were identified as camphor (13.62%), 1,8-cineol (6.00%), borneol (5.74%), viridiflorol (4.00%), and linalool (3.93%). For Rosmarinus officinalis essential oil, 48 compounds were characterized, of which the main constituents were camphor (17.09%), Z-β-ocimene (10.88%), isoborneol (9.68%), α-bisabolol (7.89%), and borneol (5.11%). While, Salvia officinalis essential oil was characterized by β-thujone (16.44%), followed by viridiflorol (10.93%), camphor (8.99%), 1,8-cineol (8.11%), trans-caryophyllene (5.85%), and α-humulene (4.69%) as the major components. Notably, results from antibacterial screening indicated that Thymus algeriensis and Salvia officinalis essential oils exhibited a strong inhibitory effect against both Escherichia coli and Staphylococcus aureus compared to Rosmarinus officinalis essential oil. Further, less activity was recorded against Pseudomonas aeruginosa for the three tested essential oils.

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