Effects of Evaporative Emissions Control Measurements on Ozone Concentrations in Brazil

In this work, the possible benefits obtained due to the implementation of evaporative emissions control measures, originating from vehicle fueling processes, on ozone concentrations are verified. The measures studied are: (1) control at the moment when the tank trucks supply the fuel to the gas stations (Stage 1); (2) control at the moment when the vehicles are refueled at the gas stations, through a device installed in the pumps (Stage 2); (3) same as the previous control, but through a device installed in the vehicles (ORVR). The effects of these procedures were analyzed using numerical modeling with the VEIN and WRF/Chem models for a base case in 2018 and different emission scenarios, both in 2018 and 2031. The results obtained for 2018 show that the implementation of Stages 1 and 2 would reduce HCNM emissions by 47.96%, with a consequent reduction of 19.9% in the average concentrations of tropospheric ozone. For 2031, the greatest reductions in ozone concentrations were obtained with the scenario without ORVR, and with Stage 1 and Stage 2 (64.65% reduction in HCNM emissions and 31.93% in ozone), followed by the scenario with ORVR and with Stage 1 and Stage 2 (64.39% reduction in HCNM emissions and 32.98% in ozone concentrations).

Nursery performance of potentially promising rootstocks for citriculture in the south of Brazil

ABSTRACT: Diversification of rootstock varieties, with consequent reduction in phytosanitary risks, has great importance to the sustainability of citrus cultivation in the south of Brazil. This study evaluated the performance of 42 rootstocks in the nursery phase to generate ‘Valencia Late’ sweet orange seedlings. Therefore, nucellar seedlings from the rootstocks under study were analyzed in relation to plant height, stem diameter, mortality rate and percentage of bud set after grafting. The experimental design was completely randomized with four replications of 20 sample units. Not adapted genotypes had high mortality rates, and the ones of both hybrids LCR x CTSW - 009 and LVK x LCR - 038 were 100% and 90%, respectively. Other 13 genotypes had mortality rates above 30%, and ‘Sunki’ mandarin was the female genitor of ten of them. ‘Swingle’ citrumelo was the rootstock with the highest development (plant height and stem diameter), associated with low mortality rate. In relation to the other rootstocks, the best results were the fast grafting diameter, associated with low mortality rate obtained by hybrids HTR - 053, LRF x (LCR x TR) - 005, CLEO x TRBN - 245, CLEO x TRSW - 287, and citrandarins ‘Indio’, ‘Riverside’ and ‘San Diego’, as well as Trifoliate orange and ‘Rangpur’ lime. In addition to Trifoliate orange, which is widely used in Rio Grande do Sul, these rootstocks have great potential in citriculture in the south of Brazil.

Application of chitosan and its derivatives in drug delivery

Drug delivery is a method of delivering drug to the patients in a way which increases the concentration of drug to the relevant site by directing the drug to the specific site of action. Polymeric drug delivery systems are mostly preferred to take the drug to the specific site of action and achieve the therapeutic effect. Among different types of biocompatible polymers, carbohydrate-based polymers or polysaccharides are the most common natural polymers with complex structures consisting of long chains of monosaccharide or disaccharide units bound by glycosidic linkages. One such polymer is chitosan which is used in drug delivery. Chitosan’s appealing properties, especially of natural origin, may mediate the protection against degradation of the biologically active substance, control the release of the drug, boost the absorption, increase the physiological action and result in a consequent reduction in the rate of administration. Another advantage of using chitosan is its chemical structure that could be modified easily which makes chitosan derivatives used for different applications. The main objective is to provide an overview on chitosan and its derivatives applications in drug delivery.

Role of Pegfilgrastim in the Supportive Care of Heavily Pretreated Multiple Myeloma in Treatment with Pomalidomide-Dexamethasone

Pegfilgrastim is a pegylated long-acting recombinant form of G-CSF that extends the half-life and allows for once-per-cycle dosing, requiring less frequent dosing than nonpegylated G-CSF. The objective of this study was to compare the efficacy and safety of pegfilgrastim in patients affected by heavily pretreated MM, treated with pomalidomide-dexamethasone, in order to determine whether a single subcutaneous injection of pegfilgrastim is as effective as daily injections of standard filgrastim, in terms of haematological toxicity, febrile neutropenic episodes, antibiotic usage and hospedalization duration. 57 patients (31 M and 26 F) were enrolled, median age at diagnosis 69 years (r. 52-84), and median age at start of treatment 76 years (r.56-90) treated with several lines of treatments (median 7, r. 2-12), every refractory to all the drugs previously received, received Pomalidomide-Dexamethasone (P 4 mg for 21 days, D 40 mg days 1,8,15,22, pegfilgrastim day +8) every 28 days, until progression. Since first course, received in domestic setting, with a very good compliance, patients performed blood counts once weekly and received, from day +8 to day +19, prophylactic oral chinolonic antibiotics and anti-fungal drugs. During neutropenia after first cycle, Filgrastim (5 μgr/kg/day for 3 days) was given if neutrophils count was <1500 x 10^9 cells/L. Median number of filgrastim administrations was 4.6 (r. 3-6); nadir neutropenia was registered after a median of 10.4 days (r. 7-14); median of nadir neutrophil count was 1.13 x 10^9 cells/L (r.0.3 - 1.5), with maximum duration of 14 days. From the second course, all patients switched to prophylaxis with pegfilgrastim (6 mg), injected subcutaneously with a single administration on day +3 independently from the neutrophil count at that time. During pegfilgrastim, neutropenia was never longer than 8 days, with a consequent reduction of neutropenia-related infections. Median nadir neutrophil count, evaluated for every patients for at least three courses of therapy (r. 3-6) registered at day +11, was 1.28 (r.0.9-2.2). Only 4 patients needed a supplement of 3 administrations of filgrastim. Pegfilgrastim was well tolerated in all patients: main side effects in our patients were mild fever and bone pain (21.2%). In patients affected by heavily pretreated MM treated with pomalidomide-dexamethasone, pegfilgrastim seems to reduce the incidence of severe neutropenia and infections and may increase the possibility to maintain the scheduled time of treatment. Disclosures Pane: AbbVie; Amgen; Novartis, GSK , Incyte: Consultancy; AbbVie; Amgen; Novartis, GSK, Incyte: Speakers Bureau; Novartis Pharma SAS;: Research Funding; AbbVie; Amgen; Novartis: Other: Travel, accommodation, expenses. Martinelli: Jazz Pharmaceuticals: Consultancy; Stemline Therapeutics: Consultancy; Astellas: Consultancy, Speakers Bureau; Roche: Consultancy; Incyte: Consultancy; Pfizer: Consultancy, Speakers Bureau; Abbvie: Consultancy; Celgene /BMS: Consultancy, Speakers Bureau; Daichii Sankyo: Consultancy.

Asbestos Removal Acceleration for New Jobs and Fossil Fuel Use Reduction for Public Health and Climate Readiness: A Contribution to the Revival of the Italian Economy Post-COVID-19

The Italian economy has been battered by the SARS-CoV-2/COVID-19 pandemic. European Union and Italian government economic recovery funds will provide special economic recovery loans aimed toward energy saving and the consequent reduction of greenhouse gas emissions (GHG) to meet the Paris Agreement climate goals. In, Italy, millions of square meters of asbestos cement (AC) roofing cover industrial and civic buildings. Removal of this asbestos containing material (ACM) would contribute to three pandemic and economic recovery objectives: job creation, reduction of greenhouse gasses (thru energy savings), and public health improvements. Though asbestos was used for its excellent thermal insulation properties, we provide calculations that demonstrate that the cement binding in AC roofing negates the asbestos insulation function. Therefore, replacing AC roofing with roofs made with alternative materials with better thermal transmission coefficients can contribute significantly to energy savings, reduce the risk of asbestos-related morbidity and mortality, and establish substantial economic activity.

Feasibility study of fluorescent lamp waste recycling by thermal desorption

The proposed Minamata Convention ban on the use of fluorescent lamps at the end of 2020, with a consequent reduction in mercury (Hg) light products, is expected to produce large amounts of discarded fluorescent bulbs. In this context, the most effective recycling options are a thermal mercury recovery system and/or aqueous solution leaching (lixiviation) to recover rare earth elements (REEs). Due to the heterogeneous nature of these wastes, a complete characterization of Hg compounds in addition to a determination of their desorption temperatures is required for their recycling. The objective of this study is to assess the feasibility of a fast cost-effective thermal characterization to ameliorate recycling treatments. A pyrolysis heating system with a heat ramping capability combined with atomic absorption spectrometry makes it possible to obtain residue data with regard to the temperature ranges needed to achieve total Hg desorption. The major drawback of these heat treatments has been the amount of Hg absorbed from the residue by the glass matrices, ranging from 23.4 to 39.1% in the samples studied. Meanwhile, it has been estimated that 70% of Hg is recovered at a temperature of 437 °C.

Hydro-geomechanical characterisation of a coastal urban aquifer using multiscalar time and frequency domain groundwater-level responses

Hydraulic properties of coastal, urban aquifers vary spatially and temporally with the complex dynamics of their hydrogeology and the heterogeneity of ocean-influenced hydraulic processes. Traditional aquifer characterisation methods are expensive, time-consuming and represent a snapshot in time. Tidal subsurface analysis (TSA) can passively characterise subsurface processes and establish hydro-geomechanical properties from groundwater head time-series but is typically applied to individual wells inland. Presented here, TSA is applied to a network of 116 groundwater boreholes to spatially characterise confinement and specific storage across a coastal aquifer at city-scale in Cardiff (UK) using a 23-year high-frequency time-series dataset. The dataset comprises Earth, atmospheric and oceanic signals, with the analysis conducted in the time domain, by calculating barometric response functions (BRFs), and in the frequency domain (TSA). By examining the damping and attenuation of groundwater response to ocean tides (OT) with distance from the coast/rivers, a multi-borehole comparison of TSA with BRF shows this combination of analyses facilitates disentangling the influence of tidal signals and estimation of spatially distributed aquifer properties for non-OT-influenced boreholes. The time-series analysed covers a period pre- and post-impoundment of Cardiff’s rivers by a barrage, revealing the consequent reduction in subsurface OT signal propagation post-construction. The results indicate that a much higher degree of confined conditions exist across the aquifer than previously thought (specific storage = 2.3 × 10−6 to 7.9 × 10−5 m−1), with implications for understanding aquifer recharge, and informing the best strategies for utilising groundwater and shallow geothermal resources.

Foliar Application of Nitrogen Affects Metabolism and Productivity of Soybean

Soybean is one of the world’s most economically important crops and several factors can affect the productivity of this culture. Among these factors is the supply of needed nitrogen, especially in the reproductive stage, as it acts in photosynthetic activity and in grain filling. In view of this, the objective of our work was to evaluate the effect of foliar application of nitrogen in different reproductive stages in soybean culture. Two sources of nitrogen were used: conventional urea and urea-formaldehyde/triazone, both applied in reproductive stages R2, R3, R4, or R5, as well as a control treatment without foliar application of nitrogen. Plants submitted to foliar application of urea-formaldehyde/triazone showed an increase in nitrogen metabolism (percentage of nitrogen derived from the atmosphere [Ndfa] and nitrate reductase activity [NR]), an increase in peroxidase (POD), and the consequent reduction in hydrogen peroxide (H2O2) in all stages of application of this treatment. When urea-formaldehyde/triazone was applied in R4, it resulted in a 7% increase in yield. The application of conventional urea in reproductive stages R4 and R5 increased nitrogen metabolism and resulted in an increase in yield by 4%. However, conventional urea reduced yield when applied in stages R2 and R3. The use of low doses of foliar nitrogen in stages R4 and R5, increased nitrogen metabolism in soybean plants. The timing of the application has a direct impact on the results with the slow-release nitrogen (urea formaldehyde /triazone) showing better results when applied in stage R4 and better results for conventional urea in R5.

HIF2α regulates the synthesis and release of epinephrine in the adrenal medulla

The adrenal gland and its hormones regulate numerous fundamental biological processes; however, the impact of hypoxia signaling on adrenal function remains poorly understood. Here, we reveal that deficiency of HIF (hypoxia inducible factors) prolyl hydroxylase domain protein-2 (PHD2) in the adrenal medulla of mice results in HIF2α-mediated reduction in phenylethanolamine N-methyltransferase (PNMT) expression, and consequent reduction in epinephrine synthesis. Simultaneous loss of PHD2 in renal erythropoietin (EPO)-producing cells (REPCs) stimulated HIF2α-driven EPO overproduction, excessive RBC formation (erythrocytosis), and systemic hypoglycemia, which is necessary and sufficient to enhance exocytosis of epinephrine from the adrenal medulla. Based on these results, we propose that the PHD2-HIF2α axis in the adrenal medulla regulates the synthesis of epinephrine, whereas in REPCs, it indirectly induces the release of this hormone. Our findings are also highly relevant to the testing of small molecule PHD inhibitors in phase III clinical trials for patients with renal anemia. Key messages HIF2α and not HIF1α modulates PNMT during epinephrine synthesis in chromaffin cells. The PHD2-HIF2α-EPO axis induces erythrocytosis and hypoglycemia. Reduced systemic glucose facilitates exocytosis of epinephrine from adrenal gland.

Gonadotrophin-releasing hormone receptors in GtoPdb v.2021.3

GnRH1 and GnRH2 receptors (provisonal nomenclature [39], also called Type I and Type II GnRH receptor, respectively [85]) have been cloned from numerous species, most of which express two or three types of GnRH receptor [85, 84, 114]. GnRH I (p-Glu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2) is a hypothalamic decapeptide also known as luteinizing hormone-releasing hormone, gonadoliberin, luliberin, gonadorelin or simply as GnRH. It is a member of a family of similar peptides found in many species [85, 84, 114] including GnRH II (pGlu-His-Trp-Ser-His-Gly-Trp-Tyr-Pro-Gly-NH2 (which is also known as chicken GnRH-II). Receptors for three forms of GnRH exist in some species but only GnRH I and GnRH II and their cognate receptors have been found in mammals [85, 84, 114]. GnRH1 receptors are expressed by pituitary gonadotrophs, where they mediate the effects of GnRH on gonadotropin hormone synthesis and secretion that underpin central control of mammalian reproduction. GnRH analogues are used in assisted reproduction and to treat steroid hormone-dependent conditions [58]. Notably, agonists cause desensitization of GnRH-stimulated gonadotropin secretion and the consequent reduction in circulating sex steroids is exploited to treat hormone-dependent cancers of the breast, ovary and prostate [58]. GnRH1 receptors are selectively activated by GnRH I and all lack the COOH-terminal tails found in other GPCRs. GnRH2 receptors do have COOH-terminal tails and (where tested) are selective for GnRH II over GnRH I. GnRH2 receptors are expressed by some primates but not by humans [88]. Phylogenetic classifications divide GnRH receptors into three [85] or five groups [129] and highlight examples of gene loss through evolution, with humans retaining only one ancient gene. The structure of the GnRH1 receptor in complex with elagolix has been elucidated [132].