Povidone Iodine Sterilization of Contaminated CAPD-Exchange Spikes

1985 ◽  
Vol 5 (1) ◽  
pp. 24-26 ◽  
Author(s):  
Thomas A. Golper ◽  
David L. Sewell ◽  
Linda West ◽  
Marge Trinklein
Keyword(s):  
Bacterial Growth ◽  
Povidone Iodine ◽  
Bacterial Suspension ◽  
Limiting Factor ◽  
Group I ◽  
Dialysis Solution ◽  
Colony Forming Units ◽  
Physiologic Saline ◽  
Group 3 ◽  
Group 2

Thirty routine CAPD exchange spikes (Travenol) were dipped in a Staphylococcus aureus suspension and then divided into three equal groups. Group I (G1) spikes were advanced from the bacterial suspension to a povidone-iodine solution for a five-minute soak; Group 2 (G2) spikes were advanced into a sterile, non-bacteriostatic, physiologic saline solution for a five-minute soak. Then G1 and G2 spikes were attached in the usual sterile fashion to dialysis bags pretreated with tryptic soy broth to enhance bacterial growth. Group 3 (G3) spikes were advanced immediately to pretreated dialysate bags. Cultures of the dialysis solution were obtained immediately after the spikes were connected to the dialysis bags, 48 hours later, and at weekly intervals for three weeks. All 10 of the G3 bags connected to spikes without soaking grew greater than 10 colony forming units/mi of S. aureus by 48 hours. All 10 of the saline soaked G2 bags also demonstrated growth at 48 hours. Only one of the 10 Gl povidone-iodine soaked spike bags grew detectable colonies of bacteria at 48 hours. We conclude that a five-minute povidone-iodine soaking of spikes contaminated with S. aureus usually will prevent bacterial growth but is not a perfect solution to the problems of spike contamination. The frequent occurrence of peritonitis remains the limiting factor to the widespread acceptance of CAPD. Oreopoulos et al proposed that 36.5% of episodes of peritonitis are secondary to contaminations at the connection site during the a CAPD bag exchange (1). Although the exact frequency is unknown, we agree that the likelihood is high that contamination at the time of the exchange is a common mode of bacterial access to the peritoneal cavity. When we established our CAPD programs we instructed our patients to change the tubing if the spike became contaminated. Patients only rarely notified us of this necessity, despite our suspicion that known contamination was occurring. Two of us asked the Bulletin's editors if one could manage spike contamination simply by soaking the spike in povidone-iodine (2). Vas recommended that the tubing be changed because simple soaking would not deal with contamination inside the lumen (3). We decided to study this issue further because we believed that patients would not comply with this recommendation.

Is oxygen supply a limiting factor for survival during rewarming from profound hypothermia?

2006 ◽  
Vol 291 (1) ◽  
pp. H441-H450 ◽  
Author(s):  
Timofei V. Kondratiev ◽  
Kristina Flemming ◽  
Eivind S. P. Myhre ◽  
Mikhail A. Sovershaev ◽  
Torkjel Tveita
Keyword(s):  
Blood Volume ◽  
Tissue Oxygenation ◽  
Limiting Factor ◽  
Myocardial Tissue ◽  
Circulating Blood Volume ◽  
Profound Hypothermia ◽  
Group 3 ◽  
Vascular Beds ◽  
Group 2 ◽  
Group 1

It has been postulated that unsuccessful resuscitation of victims of accidental hypothermia is caused by insufficient tissue oxygenation. The aim of this study was to test whether inadequate O2supply and/or malfunctioning O2extraction occur during rewarming from deep/profound hypothermia of different duration. Three groups of rats ( n = 7 each) were used: group 1 served as normothermic control for 5 h; groups 2 and 3 were core cooled to 15°C, kept at 15°C for 1 and 5 h, respectively, and then rewarmed. In both hypothermic groups, cardiac output (CO) decreased spontaneously by >50% in response to cooling. O2consumption fell to less than one-third during cooling but recovered completely in both groups during rewarming. During hypothermia, circulating blood volume in both groups was reduced to approximately one-third of baseline, indicating that some vascular beds were critically perfused during hypothermia. CO recovered completely in animals rewarmed after 1 h ( group 2) but recovered to only 60% in those rewarmed after 5 h ( group 3), whereas blood volume increased to approximately three-fourths of baseline in both groups. Metabolic acidosis was observed only after 5 h of hypothermia (15°C). A significant increase in myocardial tissue heat shock protein 70 after rewarming in group 3, but not in group 2, indicates an association with the duration of hypothermia. Thus mechanisms facilitating O2extraction function well during deep/profound hypothermia, and, despite low CO, O2supply was not a limiting factor for survival in the present experiments.

Relationships between follicle-stimulating hormone, follicle growth and ovulation rate in sheep

1996 ◽  
Vol 8 (2) ◽  
pp. 279 ◽  
Author(s):  
RC Fry ◽  
MA Driancourt
Keyword(s):  
Follicle Stimulating Hormone ◽  
Follicular Phase ◽  
Oestrous Cycle ◽  
Ovulation Rate ◽  
Follicle Growth ◽  
Group I ◽  
Group 3 ◽  
Group 2 ◽  
Group 1 ◽  
Stimulating Hormone

The changes in follicle-stimulating hormone (FSH) concentration required to affect follicle growth and ovulation rate within individual ewes were examined. Relationships between peripheral FSH concentrations during the late-luteal and follicular phase and subsequent ovulation rates were investigated in 22 ewes from 4 breeds over 3 successive cycles (Experiment 1). Ewes were grouped as follows: Group 1 (n = 6), ewes exhibiting the same ovulation rate at each oestrous cycle: Group 2 (n = 5), ewes with three different ovulation rates at each oestrous cycle; and Group 3 (n = 11), ewes with the same ovulation rate at two oestrous cycles and a different ovulation rate on one occasion. Data from ewes in Group 1 and 3 provided estimates on the variation in FSH concentrations between cycles which were not large enough to alter ovulation rate (range, 0-67% variation in FSH concentration). In Group-2 ewes, there was no consistent association between increases in ovulation rate and the proportional increases in FSH concentrations. Differences in FSH concentrations were often less than those that did not alter ovulation rate in Group-I ewes. Furthermore, only 3 of 11 Group-3 ewes demonstrated high FSH concentrations associated with high ovulation rate (or low FSH concentrations and low ovulation rate) when compared with the concentrations found at the two cycles in which ovulation rate was similar. Hence, there was little evidence that FSH concentrations during the late-luteal and follicular phase are associated with changes in ovulation rate within individual ewes. In Experiment 2, follicles of similar size obtained from the same ewe (FecBFec+ and Romanov) showed markedly different responses in vitro to graded doses of FSH as measured by aromatase activity. It is concluded that, within a ewe, the large variability between gonadotrophin-dependent follicles in their requirement for FSH prevented the expression of any thresholds of ovarian response to FSH.

Effects of Therapeutic Irradiation Delivered in Early Childhood Upon Subsequent Lung Function

PEDIATRICS ◽  
1975 ◽  
Vol 55 (4) ◽  
pp. 507-516
Author(s):  
Mary Ellen B. Wohl ◽  
N. Thorne Griscom ◽  
Demetrius G. Traggis ◽  
Norman Jaffe
Keyword(s):  
Lung Volume ◽  
Actinomycin D ◽  
Wilms Tumor ◽  
Tumor Therapy ◽  
Lung Growth ◽  
Long Term Effects ◽  
Group I ◽  
Group 3 ◽  
Group 2 ◽  
Therapeutic Irradiation

To determine the long-term effects of therapeutic pulmonary irradiation and treatment with actinomycin D during a period of lung growth, 12 patients treated for Wilms' tumor metastatic to the lung and 8 patients treated for Wilms' tumor with no evidence of pulmonary metastases were studied 7 to 14 years after their initial tumor therapy. All patients had received irradiation to the tumor bed and treatment with actinomycin D. Group I had received a single course of bilateral pulmonary irradiation; group 2 had received additional pulmonary irradiation and/or thoracic surgery; group 3 had received no therapeutic irradiation directed primarily to the chest. Total lung capacity (TLC) averaged 71% of predicted value in group 1,58% in group 2, and 94% in group 3. Diffusing capacity in groups 1 and 2 was reduced to the same extent as lung volume. Quasi-static pressure-volume relationships, studied in three of six patients in group 1, were within the normal range when lung volume was expressed as percentage of observed TLC. Airway resistance, evaluated by spirometry, maximum expiratory flow-volume curves, and resistance of the total respiratory system, was normal or reduced. The data support the hypothesis that therapeutic irradiation during a period of lung growth primarily affects the lung parenchyma and produces a decrease in subsequent size of both the lung and chest wall. No effect of actinomycin D alone upon the lung could be demonstrated.

scholarly journals Esthetic restorative materials and glass ionomer cements: Influence of acidic drink exposure on bacterial adhesion

2018 ◽  
Vol 12 (02) ◽  
pp. 204-209 ◽  
Author(s):  
Claudio Poggio ◽  
Lodovico Vialba ◽  
Riccardo Marchioni ◽  
Marco Colombo ◽  
Giampiero Pietrocola
Keyword(s):  
Bacterial Adhesion ◽  
Soft Drink ◽  
Cylindrical Sample ◽  
Bacterial Suspension ◽  
Glass Ionomer Cements ◽  
Glass Ionomer ◽  
Restorative Materials ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

ABSTRACT Objective: The purpose of this research was to evaluate and compare bacterial adhesion on five esthetic restorative materials, three glass ionomer cements (GIC), and two GIC with coat. All the materials were considered after acidic drink exposure. Materials and Methods: Thirty cylindrical sample of each of the 10 materials were prepared and then divided into three groups: group 1 (baseline), Group 2 (1 day in acidic soft drink), and Group 3 (7 days in acidic soft drink). Bacterial suspension of Streptococcus mutans was cultured and deposited onto each material, and the adhesion was evaluated through the colony-forming units determination. One-way ANOVA and Bonferroni's post hoc tests were applied to estimate significant differences between the esthetic materials. Results: The highest amount of S. mutans was recorded in Group 3 and the lowest in Group 1 (baseline). In general, the GIC showed bacterial adhesion values higher than the ones related to composites both in Group 2 than in Group 3. Acidic soft drinks lead a time-dependent degradation of restorative materials causing an increase of the surface rugosity. In fact, a general increase in S. mutans cells adhesion to treated samples was observed. Conclusions: The use of acidic soft drink resulted in a degradation of the surface layer of the restorative material with consequent increase of bacterial adhesion. The GIC can be considered a more friendly environment for bacterial adhesion. This is true in particular if acid substances have already deteriorated the surface.

scholarly journals Effects of water quality and stocking density on growth performance of juvenile cod (Gadus morhua L.)

2006 ◽  
Vol 63 (2) ◽  
pp. 326-334 ◽  
Author(s):  
Björn Björnsson ◽  
Sólveig R. Ólafsdóttir
Keyword(s):  
Growth Rate ◽  
Gadus Morhua ◽  
Stocking Density ◽  
Ammonia Nitrogen ◽  
Limiting Factor ◽  
Negative Effects ◽  
Total Ammonia ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Abstract In a 5-month experiment where groups of juvenile cod were reared in a flow-through system at low density (Group 1) and in a recirculating system at low (Group 2) and high densities (Group 3), the recirculated water had negative effects on growth rate, nutritional condition, and mortality. After the first month, mean weight was significantly larger in Group 1 than in the other two groups. The effect of stocking density on mean weight was not significant until the end of the experiment, when densities were 9.0 and 48.3 kg m−3 in Groups 2 and 3, respectively. Initial mean weight of fish was 37.3 g and at the termination of the experiment mean weights were 225.2, 181.8, and 167.9 g in Groups 1, 2, and 3, respectively. After 5 months, mean condition factors were 1.074, 0.965, and 0.946, mean liver indices 9.5, 9.0, and 7.6, and mean mortalities over the course of the experiment were 1.0%, 5.1%, and 2.4% in Groups 1, 2, and 3, respectively. Water temperature (10.4–10.6°C), pH (7.2–7.4) and oxygen concentration (8–10 mg l−1) were similar among groups. Concentration of total ammonia nitrogen (TAN) was similar among groups for the first half of the experiment (0.3–0.4), but during the last month it was 0.6, 1.3, and 1.5 mg l−1 in Groups 1, 2, and 3, respectively. There was a negative correlation (r2 = 0.48, n = 36) between relative growth rate and TAN, suggesting that ammonia may have been a limiting factor in the recirculating system. The apparent threshold limit of TAN for reduced growth was approximately 1 mg l−1.

scholarly journals The Effect of Mupirocin- and Fusidic Acid-nasal Packings, Placed after Septoplasty, on the Nasal Bacterial Profile

2016 ◽  
Vol 7 (4) ◽  
pp. ar.2016.7.0181
Author(s):  
Suat Bilici ◽  
Yusuf Muhammed Durna ◽  
Ozgur Yigit ◽  
Aysel Karatas ◽  
Cansu Cimen ◽  
...  
Keyword(s):  
Growth Rate ◽  
Bacterial Growth ◽  
Fusidic Acid ◽  
Prophylactic Antibiotic ◽  
Acid Group ◽  
Preoperative Period ◽  
Gram Positive ◽  
Methicillin Resistant ◽  
Group 3 ◽  
Group 2

Aim To examine the effects, after septoplasty, of local antibiotic pomades as an alternative to prophylactic antibiotic use, which is a controversial matter among the otolaryngologists, on nasal flora and bacterial growth. Material and Method Nasal packings placed after septoplasty surgery to provide septal stabilization and bleeding control pose a risk in terms of infection. In this study, 106 patients, who were examined by comparing mupirocin- and fusidic acid-soaked packings and antibiotic-free packings, were divided into three groups. Nasal cultures were obtained from each patient twice, before the surgery and on the second day immediately after the packings were removed, and the culture results were statistically compared. Results In the mupirocin group (group 2), postoperative normal flora growth rate was significantly higher than in the fusidic acid group (group 3) and the antibiotic-free group (group 1) (p < 0.024). In the mupirocin group (group 2), the gram-positive growth rate in the postoperative period showed a significant decrease when compared when the preoperative period (p < 0.05) (5.7%). In the fusidic acid group (group 3), the postoperative gram-positive rate showed a significant decrease compared with the preoperative period (p < 0.05). In group 2, the postoperative methicillin-resistant Staphylococcus aureus rate showed a significant decrease than in the preoperative period (2.9%) (p < 0.05). Similarly, in group 3, the postoperative methicillin-resistant S. aureus rate showed a significant decrease compared with that of the preoperative period (11.1%) (p < 0.05). Conclusion Use of mupirocin- and fusidic acid-soaked nasal packings after septoplasty significantly decreased, especially, postoperative gram-positive bacterial growth in nasal cultures. Although systemic antibiotherapy was not administered, the lack of local and systemic infection findings was an important result that we obtained in terms of clinical use. Usage advantages of mupirocin and fusidic acid soaked packings are an easily applicable, cost-effective, and safe method.

scholarly journals Estimation of Micronutrients (Magnesium and Iron) in Periodontitis Patients with and without Type-2 Diabetes Mellitus

2021 ◽  
Vol 10 (22) ◽  
pp. 1645-1649
Author(s):  
Pallavi Samatha Yalamanchili ◽  
Nirupa Elisetti ◽  
Janaki Kesipeddy ◽  
Bilwa Bindu A ◽  
Bhagyasree Vegunta
Keyword(s):  
Diabetes Mellitus ◽  
Statistical Significance ◽  
Serum Magnesium ◽  
Serum Levels ◽  
Diabetic Patients ◽  
Healthy Controls ◽  
Type Ii ◽  
Group I ◽  
Group 3 ◽  
Group 2

BACKGROUND The focus of this study was to assess the relationship of nutrients (magnesium, iron) in periodontitis and diabetic patients. Serum magnesium (Mg) and iron (Fe) levels were evaluated in periodontitis patients and were compared between diabetic patients with periodontitis and healthy controls. METHODS Total sixty subjects (18 females and 42 males) were recruited in this cross-sectional study and were divided into three groups - Group I (healthy subjects), Group II (chronic generalized periodontitis), Group III (Type II diabetes mellitus and chronic generalized periodontitis). Loe and Silness gingival index and probing pocket depth were used for categorizing chronic generalized periodontitis patients. Serum levels of magnesium and iron were estimated and then compared among the groups. Statistical analysis was done using one-way analysis of variance (ANOVA) and Newmann-Keuls multiple post-hoc procedures. Statistical significance was accepted for P ≤ 0.05. RESULTS Mean serum levels of magnesium (Mg) were 0.89 in Group 3 (Type II DM + CGP), 1.01 in Group 2 (CGP) against 1.52 in Group 1 (Control), which were statistically significant. Mean Iron (Fe) levels showed 165.56 in Group 3 (Type II DM + CGP), 137.77 in Group 2 (CGP) against 78.17 (Control) which is also statistically significant. CONCLUSIONS In this study, we found statistically significant decrease in magnesium and increase in serum iron levels in Group 3 (Type II DM + CGP) compared to Group 2 (CGP) and healthy controls. These findings suggest that trace elements also play an important role in the progression of periodontal disease. KEY WORDS Chronic Periodontitis, Diabetes Mellitus, Iron, Magnesium

scholarly journals Comparison of active calf muscle stretching versus ankle mobilisation on low back pain and lumbar flexibility in pronated foot subjects

2017 ◽  
Vol 4 (6) ◽  
pp. 1870 ◽  
Author(s):  
K. Vadivelan ◽  
J. S. Poyyamozhi ◽  
G. Dinesh Kumar ◽  
C. Rajan Rushender
Keyword(s):  
Low Back Pain ◽  
Back Pain ◽  
Calf Muscle ◽  
P Value ◽  
Lumbar Region ◽  
Low Back ◽  
Group I ◽  
Significant Difference ◽  
Group 3 ◽  
Group 2

Background: Low back pain is the common symptom of the lumbar region that more than 80 percent of people experience in their lifetime. Methods: Quasi-experimental study was conducted with three intervention groups. Ankle mobilization (Group I), Active stretching of calf muscle (Group 2), No Intervention (Group 3). This study was carried out in the Department of Physiotherapy at SRM University, Chennai. 21 subjects were included in the study, with 7 subjects in each group. Results: Median age was found to be 20 (19–21), 19 (18–20) and 21 (20–22) in group 1, group 2 and group 3 respectively and no significant difference was seen between the groups, p value 0.11) There was a significant improvement seen in visual analogue scale, in both the intervention groups before and after intervention. The median and IQR for ankle mobilization group pre and post intervention was 6 (IQR, 5–6) and 4 (IQR, 3–5) respectively, with a p value <0.01. Conclusions: Overall results have shown that both the intervention groups have similar effects in reducing lower back pain and improving lumbar flexion, compared to the control group. 

scholarly journals Comparative study of the effectiveness and residual effect of preoperative antisepsis with 4% chlorhexidine gluconate associated with 70% ethyl alcohol and 0.5% alcoholic chlorhexidine gluconate in dogs

2021 ◽  
Vol 15 (2) ◽  
pp. 161-165
Author(s):  
Joseneia Boeing ◽  
Amanda Marcondes Pires ◽  
Danielli Aparecida Lavelli ◽  
Gabriel Godoi de Moraes ◽  
Paola Castro Moraes ◽  
...  
Keyword(s):  
Comparative Study ◽  
Ethyl Alcohol ◽  
Bacterial Growth ◽  
Residual Effect ◽  
Chlorhexidine Gluconate ◽  
Alcohol Group ◽  
Skin Bacteria ◽  
Colony Forming Units ◽  
Group 2 ◽  
Group 1

The effectiveness of antisepsis of surgical sites in 20 animals (canine species) was compared and subdivided into two groups, using 4% chlorhexidine gluconate associated with alcohol (group 1) and 0.5% chlorhexidine gluconate (group 2). The samples were collected through skin swab after trichotomy (T1), after definitive antisepsis (T2) and one hour after the use of antiseptic (T3), and then submitted to the count of colony forming units (CFU). In both groups, bacterial growth occurred in T1; in T2, the reduction of CFUs was significant for both groups (G1 and G2); however, if we consider absolute values, we can see in T1 a greater amount of CFUs in G2, and when evaluating the results of T2, we can see values which are very similar between G1 and G2, which may suggest greater efficiency of G2 in initial times after antisepsis. In T3, the reduction of CFUs was more effective for G1, suggesting a greater residual effect when compared to G2. Both antiseptic protocols were effective as they significantly reduced the number of skin bacteria, both in T2 and T3.

Parenteral Hyperalimentation in Patients with Gastrointestinal Cancer

1978 ◽  
Vol 64 (4) ◽  
pp. 407-418 ◽  
Author(s):  
Federico Bozzetti
Keyword(s):  
Postoperative Complications ◽  
Parenteral Nutrition ◽  
Gastrointestinal Cancer ◽  
Locally Advanced ◽  
Gastrointestinal Surgery ◽  
Successful Outcome ◽  
Gastrointestinal Malignancy ◽  
Group I ◽  
Group 3 ◽  
Group 2

Sixty-six patients with locally advanced or diffuse gastrointestinal cancer or suffering from major complications due to surgery or radiation therapy, were treated with continuous parenteral hyperalimentation at the Istituto Nazionale Tumori of Milan for a cumulative period of 2101 study-days. Patients were divided into 4 groups: Group 1, malnourished patients with advanced gastrointestinal malignancy; Group 2, patients with gastrointestinal fistulae due to simple surgical complications or to radiation injury of the bowel; Group 3, patients with major postoperative complications; Group i, surgical patients with gastric or colo-rectal carcinoma treated preoperatively. Mean infusional regime for the various groups included 42–56 Cal/kg/day and 1.5 to 2.4 g amino acid/kg/day, and the duration of the treatment ranged from 7 to 144 days. The results obtained show that protein calorie depletion of cancer patients may depend on malnutrition and that it can be reversed by parenteral nutrition, in patients, that are candidates for surgical treatment or those who qualify for chemotherapy and/or radiotherapy. Parenteral nutrition has a fundamental role in patients with fistulae, even if much attention must be paid to the external care of the fistula and the wound. In addition, nutritional support by intravenous feeding has proven essential for a successful outcome of patients with major postoperative complications. Preoperative protein repletion and central venous nutrition in patients who require gastrointestinal surgery represent a modern advance in the field of cancer surgery.

Sign in / Sign up

Export Citation Format

Share Document