Vitamin D and Vitamin-D-Binding Protein Kinetics in Patients Treated with Continuous Ambulatory Peritoneal Dialysis (CAPD)

Serum and dialysate levels of 25-hydroxycholecalciferol (25-0HD3), 1,25 dihydrox ycholecalciferol (1,25(0H)2D3), and vitamin-D-binding protein (DBP) were measured in 14 patients undergoing continuous ambulatory peritoneal dialysis (CAPD). Serum levels of 25-0HD3 and DBP were within normal range (29.1 ± 22.9 nmollL and 5.9 ± 1.1 μmol/L, respectively). Serum levels of 1,25-(0H)2D3 were subnormal in all «16 pmol/L) but one. In 5 patients, dialysate concentrations of 25-0HD3 were 2.3 ± 0.9 nmol/L, the rest had levels <1.0 nmol/L. Small quantities of 1,25-(0H)2D3 were found in the dialysate effluents. DBP could be detected in the dialysate in all patients (0.24 ± 0.06 μmol/L). Mass transfer (MT) of 25-0HD3 and DBP were respectively -10.4 ± 8.3 nmol/24 h and -1.46 ± 0.46 μmol/24 h. Peritoneal clearances of 25-0HD3 and DBP were low (0.40 ± 0.37 mL/min and 0.18 ± 0.06 mL/ min, respectively. We conclude that CAPD leads to losses of 25-0HD3 and DBP. However, the peritoneal loss of DBP is well compensated and does not result in serum deficiency. Serum 25-0HD3 levels did not correlate with time on CAPD.

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Effect of Oral Calcidiol Treatment on Its Serum Levels and Peritoneal Losses

Peritoneal Dialysis International ◽

10.1177/089686089501500113 ◽

1995 ◽

Vol 15 (1) ◽

pp. 65-70 ◽

Cited By ~ 2

Author(s):

María E. Martinez ◽

Maria T. Del Campo ◽

Maria J. Sánchez-Cabezudo ◽

Gloria Balaguer ◽

Ana Rodriguez-Carmona ◽

...

Keyword(s):

Mass Transfer ◽

Peritoneal Dialysis ◽

Transfer Coefficient ◽

Mass Transfer Coefficient ◽

Continuous Ambulatory Peritoneal Dialysis ◽

Study Design ◽

Total Protein ◽

Serum Levels ◽

Normal Range ◽

Plasma Ratio

Objectives To evaluate calcidiol serum levels in a group of continuous ambulatory peritoneal dialysis (CAPO) patients and the effect of oral calcidiol treatment on serum levels and peritoneal losses. Study design Twenty patients (13 female, 7 male) were studied for 12 –60 months. Their ages ranged 22 72 years (mean 46±15). Serum calcidiol, total protein and urea were determined at baseline and after the administration per os of 0.133 mcg/day of calcidiol for 10 days. At the same time, calcidiol and total protein were measured in peritoneal effluent at baseline and at 5,10, and 40 days after starting this treatment. Results A significant and direct correlation between the calcidiol dialysis/plasma ratio and the peritoneal protein losses was found, both before and 40 days after calcidiol administration when calcidiol serum levels were the lowest. As calcidiol serum levels rose to the normal range in the course of the study, peritoneal losses of this metabolite increased slightly and correlated with calcidiol serum levels and urea mass transfer coefficient (MTC); the significant correlation between calcidiol serum levels and peritoneal protein losses disappeared. Conclusions When serum calcidiol levels are low, calcidiol peritoneal losses in patients on CAPO correlate with protein peritoneal losses. However, when serum calcidiollevels rise, the calcidiol peritoneal losses correlate with calcidiol serum levels and urea MTC, and not with protein peritoneal losses.

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Evaluation of vitamin D-binding protein and vitamin D metabolite loss in children on continuous ambulatory peritoneal dialysis

Bone and Mineral ◽

10.1016/0169-6009(92)90788-f ◽

1992 ◽

Vol 17 (3) ◽

pp. 389-398 ◽

Cited By ~ 5

Author(s):

Wen-tse Chen ◽

Kanji Yamaoka ◽

Shigeo Nakajima ◽

Yoshihisa Tanaka ◽

Takehisa Yamamoto ◽

...

Keyword(s):

Vitamin D ◽

Peritoneal Dialysis ◽

Continuous Ambulatory Peritoneal Dialysis ◽

Binding Protein ◽

Vitamin D Binding Protein

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Vitamin D homeostasis is compromised due to increased urinary excretion of the 25-hydroxycholecalciferol-vitamin D-binding protein complex in the Zucker diabetic fatty rat

10.31274/etd-180810-670 ◽

2011 ◽

Author(s):

Rachel Lynn Anderson

Keyword(s):

Vitamin D ◽

Urinary Excretion ◽

Protein Complex ◽

Binding Protein ◽

Vitamin D Binding Protein ◽

Zucker Diabetic Fatty Rat ◽

25 Hydroxycholecalciferol

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Increased vitamin D binding protein levels are associated with irritable bowel syndrome

Turkish Journal of Biochemistry ◽

10.1515/tjb-2020-0305 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Elif Börekci ◽

Mahmut Kılıç ◽

Zeynep Ozan ◽

Hasan Börekci ◽

Tekin Yıldırım ◽

...

Keyword(s):

Vitamin D ◽

Logistic Regression ◽

Irritable Bowel Syndrome ◽

Regression Analysis ◽

Vitamin B12 ◽

Logistic Regression Analysis ◽

Binding Protein ◽

Serum Levels ◽

Vitamin D Binding Protein ◽

Irritable Bowel

Abstract Objectives There is no reliable and valid biomarker to identify Irritable bowel syndrome (IBS) and its subtypes. The aim of this study is to explore potential serum biomarkers that may be associated with IBS subtypes, particularly in the vitamin D pathway. Methods The study population comprised 75 IBS patients and 79 controls. Patients divided into IBS subtypes. Routine biochemical parameters, 25-OH-vitamin D, vitamin D binding protein (VDBP) and vitamin D receptor (VDR) serum levels were compared between IBS subtypes and controls. Factors related to IBS subtypes were examined by multivariate logistic regression analysis. Results Vitamin D levels were lower; VDBP and VDR were higher in all IBS patients than in controls (p<0.001; 0.047 and 0.029, respectively). According to logistic regression analysis, VDBP was a disease-related parameter as much as vitamin D in all IBS subtypes. C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR) were higher especially in diarrhea-dominant IBS (IBS-D) (p=0.041; 0.046) and vitamin B12 were significantly lower in constipation-dominant IBS (IBS-C) (p=0.001). Conclusions Increased VDBP levels were associated with all IBS subtypes. Patients, especially in IBS-D, had higher serum levels of VDBP, CRP and ESR. Vitamin B12 deficiency, which we consider as a result of the disease, was more common in IBS-C.

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Reduction in circulating vitamin D binding protein in patients with multiple sclerosis

BMC Neurology ◽

10.1186/s12883-021-02200-0 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Zhila Maghbooli ◽

Abolfazl Omidifar ◽

Tarlan Varzandi ◽

Tayebeh Salehnezhad ◽

Mohammad Ali Sahraian

Keyword(s):

Multiple Sclerosis ◽

Vitamin D ◽

Binding Protein ◽

Serum Levels ◽

Causative Role ◽

Vitamin D Binding Protein ◽

Control Groups ◽

Current Case ◽

Vitamin D Levels ◽

And Control

Abstract Background In this study, we aimed to determine the risk association between vitamin D binding protein (VDBP) polymorphism in patients with multiple sclerosis (MS) in a MS biobank and the difference in VDBP serum levels in MS patients who were recently diagnosed. Method The current case-control study was performed on 296 MS patients and 313 controls. Thereafter, two common missense VDBP polymorphisms, named rs7041and rs4588, were evaluated in all the participants. Serum levels of vitamin D and vitamin D binding protein were assessed in 77 MS patients who were diagnosed since one year ago and in 67 healthy people who were matched in terms of age and sex. Result The frequency distributions of VDBP genotypes and alleles of SNP rs7041 and rs4588 were observed to be similar in both the MS and control groups (p > 0.05). The VDBP haplotypes, as Gc2/Gc2, Gc1/Gc1, and Gc1/Gc2, were found to be similar in the MS and control groups (p > 0.05). In subgroup analysis, circulating VDBP was lower in MS patients (Ln-VDBP (μgr/ml): 3.64 ± 0.91 vs. 5.31 ± 0.77, p = 0.0001) even after adjusting for vitamin D levels, body mass index, and taking vitamin D supplement. There was no significant association between VDBP haplotypes and vitamin D levels in the two groups. Conclusion The present study suggested an association between lower levels of circulating VDBP and multiple sclerosis in newly diagnosed patients. However, the VDBP causative role in the development of MS is still unclear, so it needs more studies.

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Serum levels of vitamin D, vitamin D-binding protein and vitamin D receptor in migraine patients from central Anatolia region

International Journal of Clinical Practice ◽

10.1111/ijcp.12456 ◽

2014 ◽

Vol 68 (10) ◽

pp. 1272-1277 ◽

Cited By ~ 15

Author(s):

A. Celikbilek ◽

A. Y. Gocmen ◽

G. Zararsiz ◽

N. Tanik ◽

H. Ak ◽

...

Keyword(s):

Vitamin D ◽

Vitamin D Receptor ◽

Binding Protein ◽

Serum Levels ◽

Central Anatolia ◽

Vitamin D Binding Protein

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Vitamin D homeostasis is compromised due to increased urinary excretion of the 25-hydroxycholecalciferol-vitamin D-binding protein complex in the Zucker diabetic fatty rat

AJP Endocrinology and Metabolism ◽

10.1152/ajpendo.00218.2010 ◽

2010 ◽

Vol 299 (6) ◽

pp. E959-E967 ◽

Cited By ~ 36

Author(s):

R. L. Anderson ◽

S. B. Ternes ◽

K. A. Strand ◽

M. J. Rowling

Keyword(s):

Vitamin D ◽

Urinary Excretion ◽

Binding Protein ◽

Serum Vitamin ◽

Vitamin D Status ◽

Vitamin D Binding Protein ◽

Renal Reabsorption ◽

Vitamin D Levels ◽

Zucker Diabetic Fatty Rats ◽

25 Hydroxycholecalciferol

Altered serum concentrations of the major circulating form of vitamin D [25-hydroxycholecalciferol (25D3)] and its active hormone derivative [1,25-dihydroxycholecalciferol (1,25D3)] have been linked to non-insulin-dependent diabetes mellitus (NIDDM). However, a mechanistic basis for this occurrence has not been fully elucidated. Normally, renal reabsorption of vitamin D-binding protein-bound 25D3 absolutely requires receptor-mediated endocytosis via a receptor complex containing megalin, cubilin, and disabled-2 (Dab2), whereas an absence of megalin or its endocytic partners can lead to a marked urinary loss of 25D and severe vitamin D deficiency. Therefore, we hypothesized that reduced serum vitamin D status in NIDDM may be due to reduced expression of megalin and/or its endocytic partners and increased urinary excretion of protein-complexed 25D3. In the present study, we utilized Zucker diabetic fatty Rats (ZDF) to demonstrate that renal reuptake of the 25D3-DBP complex was compromised in ZDF animals, which was reflected by a reduction in expression of megalin and Dab2. Moreover, serum levels of both 25D3 and 1,25D3 were reduced, and urinary 25D3, 1,25D3, and DBP excretion were elevated in the ZDF animals compared with their lean controls regardless of vitamin D levels in the diet. Taken together, these are the first reports to our knowledge that associate compromised renal reabsorption of the 25D3-DBP complex with expression of megalin and its endocytic partners in NIDDM, which in turn can lead to compromised vitamin D status.

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Vitamin D-Binding Protein (Gc-Globulin) in Acute Liver Failure in Children

Diagnostics ◽

10.3390/diagnostics10050278 ◽

2020 ◽

Vol 10 (5) ◽

pp. 278

Author(s):

Alina Grama ◽

Lucia Burac ◽

Cornel Olimpiu Aldea ◽

Bogdan Bulata ◽

Dan Delean ◽

...

Keyword(s):

Vitamin D ◽

Liver Failure ◽

Acute Liver Failure ◽

Binding Protein ◽

Area Under The Curve ◽

Serum Levels ◽

Control Group ◽

Vitamin D Binding Protein ◽

Disease Outcomes ◽

Poor Outcomes

This study aimed to analyse vitamin D-binding protein (Gc-globulin) serum levels in acute liver failure (ALF) in children in relation to disease outcomes and correlations with other known markers used to evaluate the severity of ALF. Our study included 34 children (mean age 4.87 ± 5.30 years) with ALF of different causes (metabolic, 26.47%; autoimmune, 23.53%; toxic, 20.59%; infection, 17.65%; unknown, 11.76%) and 30 children without any liver injury (mean age 6.11 ± 4.26 years). The outcome was poor in 14 patients (41.18%), including one child with liver transplantation (2.94%). Serum Gc-globulin levels were significantly lower in ALF patients compared to the control group (151.57 ± 171.8 mg/L vs. 498.63 ± 252.50 mg/L; p < 0.000001), with an optimum cut-off of 163.5 mg/L (Area Under the Curve, AUC, 0.8921; sensitivity, 76.50%; specificity, 100%). Levels were also lower in patients with poor outcomes compared to survivors (59.34 ± 33.73 mg/L vs. 216.12 ± 199.69 mg/L; p < 0.0001), with an optimum cut-off 115 mg/L (AUC, 0.7642; sensitivity, 100%; specificity, 50%). Gc-globulin serum levels were variable according to ALF aetiology, i.e., lower in metabolic, infectious, or unknown causes compared to autoimmune and toxic causes. Gc-globulin serum levels were decreased in children with ALF and lower in those with poor outcomes compared with survivors. Gc-globulin serum levels were correlated with other known parameters used to evaluate the severity of ALF and could help to identify patients at high risk for poor outcomes.

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