scholarly journals Increased vitamin D binding protein levels are associated with irritable bowel syndrome

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Elif Börekci ◽  
Mahmut Kılıç ◽  
Zeynep Ozan ◽  
Hasan Börekci ◽  
Tekin Yıldırım ◽  
...  
Keyword(s):  
Vitamin D ◽  
Logistic Regression ◽  
Irritable Bowel Syndrome ◽  
Regression Analysis ◽  
Vitamin B12 ◽  
Logistic Regression Analysis ◽  
Binding Protein ◽  
Serum Levels ◽  
Vitamin D Binding Protein ◽  
Irritable Bowel

Abstract Objectives There is no reliable and valid biomarker to identify Irritable bowel syndrome (IBS) and its subtypes. The aim of this study is to explore potential serum biomarkers that may be associated with IBS subtypes, particularly in the vitamin D pathway. Methods The study population comprised 75 IBS patients and 79 controls. Patients divided into IBS subtypes. Routine biochemical parameters, 25-OH-vitamin D, vitamin D binding protein (VDBP) and vitamin D receptor (VDR) serum levels were compared between IBS subtypes and controls. Factors related to IBS subtypes were examined by multivariate logistic regression analysis. Results Vitamin D levels were lower; VDBP and VDR were higher in all IBS patients than in controls (p<0.001; 0.047 and 0.029, respectively). According to logistic regression analysis, VDBP was a disease-related parameter as much as vitamin D in all IBS subtypes. C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR) were higher especially in diarrhea-dominant IBS (IBS-D) (p=0.041; 0.046) and vitamin B12 were significantly lower in constipation-dominant IBS (IBS-C) (p=0.001). Conclusions Increased VDBP levels were associated with all IBS subtypes. Patients, especially in IBS-D, had higher serum levels of VDBP, CRP and ESR. Vitamin B12 deficiency, which we consider as a result of the disease, was more common in IBS-C.

scholarly journals Reduction in circulating vitamin D binding protein in patients with multiple sclerosis

BMC Neurology ◽  
2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Zhila Maghbooli ◽  
Abolfazl Omidifar ◽  
Tarlan Varzandi ◽  
Tayebeh Salehnezhad ◽  
Mohammad Ali Sahraian
Keyword(s):  
Multiple Sclerosis ◽  
Vitamin D ◽  
Binding Protein ◽  
Serum Levels ◽  
Causative Role ◽  
Vitamin D Binding Protein ◽  
Control Groups ◽  
Current Case ◽  
Vitamin D Levels ◽  
And Control

Abstract Background In this study, we aimed to determine the risk association between vitamin D binding protein (VDBP) polymorphism in patients with multiple sclerosis (MS) in a MS biobank and the difference in VDBP serum levels in MS patients who were recently diagnosed. Method The current case-control study was performed on 296 MS patients and 313 controls. Thereafter, two common missense VDBP polymorphisms, named rs7041and rs4588, were evaluated in all the participants. Serum levels of vitamin D and vitamin D binding protein were assessed in 77 MS patients who were diagnosed since one year ago and in 67 healthy people who were matched in terms of age and sex. Result The frequency distributions of VDBP genotypes and alleles of SNP rs7041 and rs4588 were observed to be similar in both the MS and control groups (p > 0.05). The VDBP haplotypes, as Gc2/Gc2, Gc1/Gc1, and Gc1/Gc2, were found to be similar in the MS and control groups (p > 0.05). In subgroup analysis, circulating VDBP was lower in MS patients (Ln-VDBP (μgr/ml): 3.64 ± 0.91 vs. 5.31 ± 0.77, p = 0.0001) even after adjusting for vitamin D levels, body mass index, and taking vitamin D supplement. There was no significant association between VDBP haplotypes and vitamin D levels in the two groups. Conclusion The present study suggested an association between lower levels of circulating VDBP and multiple sclerosis in newly diagnosed patients. However, the VDBP causative role in the development of MS is still unclear, so it needs more studies.

Serum levels of vitamin D, vitamin D-binding protein and vitamin D receptor in migraine patients from central Anatolia region

2014 ◽  
Vol 68 (10) ◽  
pp. 1272-1277 ◽  
Author(s):  
A. Celikbilek ◽  
A. Y. Gocmen ◽  
G. Zararsiz ◽  
N. Tanik ◽  
H. Ak ◽  
...  
Keyword(s):  
Vitamin D ◽  
Vitamin D Receptor ◽  
Binding Protein ◽  
Serum Levels ◽  
Central Anatolia ◽  
Vitamin D Binding Protein

scholarly journals Vitamin D-Binding Protein (Gc-Globulin) in Acute Liver Failure in Children

Diagnostics ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 278
Author(s):  
Alina Grama ◽  
Lucia Burac ◽  
Cornel Olimpiu Aldea ◽  
Bogdan Bulata ◽  
Dan Delean ◽  
...  
Keyword(s):  
Vitamin D ◽  
Liver Failure ◽  
Acute Liver Failure ◽  
Binding Protein ◽  
Area Under The Curve ◽  
Serum Levels ◽  
Control Group ◽  
Vitamin D Binding Protein ◽  
Disease Outcomes ◽  
Poor Outcomes

This study aimed to analyse vitamin D-binding protein (Gc-globulin) serum levels in acute liver failure (ALF) in children in relation to disease outcomes and correlations with other known markers used to evaluate the severity of ALF. Our study included 34 children (mean age 4.87 ± 5.30 years) with ALF of different causes (metabolic, 26.47%; autoimmune, 23.53%; toxic, 20.59%; infection, 17.65%; unknown, 11.76%) and 30 children without any liver injury (mean age 6.11 ± 4.26 years). The outcome was poor in 14 patients (41.18%), including one child with liver transplantation (2.94%). Serum Gc-globulin levels were significantly lower in ALF patients compared to the control group (151.57 ± 171.8 mg/L vs. 498.63 ± 252.50 mg/L; p < 0.000001), with an optimum cut-off of 163.5 mg/L (Area Under the Curve, AUC, 0.8921; sensitivity, 76.50%; specificity, 100%). Levels were also lower in patients with poor outcomes compared to survivors (59.34 ± 33.73 mg/L vs. 216.12 ± 199.69 mg/L; p < 0.0001), with an optimum cut-off 115 mg/L (AUC, 0.7642; sensitivity, 100%; specificity, 50%). Gc-globulin serum levels were variable according to ALF aetiology, i.e., lower in metabolic, infectious, or unknown causes compared to autoimmune and toxic causes. Gc-globulin serum levels were decreased in children with ALF and lower in those with poor outcomes compared with survivors. Gc-globulin serum levels were correlated with other known parameters used to evaluate the severity of ALF and could help to identify patients at high risk for poor outcomes.

Neutrophil CD64 combined with PCT, CRP and WBC improves the sensitivity for the early diagnosis of neonatal sepsis

2016 ◽  
Vol 54 (2) ◽  
Author(s):  
Ai-Ping Yang ◽  
Jun Liu ◽  
Lei-He Yue ◽  
Hong-Qi Wang ◽  
Wen-Juan Yang ◽  
...  
Keyword(s):  
Logistic Regression ◽  
Regression Analysis ◽  
Early Diagnosis ◽  
Logistic Regression Analysis ◽  
Neonatal Sepsis ◽  
Serum Levels ◽  
Diagnostic Value ◽  
Serum Biomarkers ◽  
Sepsis Group ◽  
Neutrophil Cd64

AbstractThe aim of this study was to determine whether neutrophil CD64 (nCD64) combined with procalcitonin (PCT), C-reactive protein (CRP) and white blood cell count (WBC) can increase the sensitivity and accuracy of neonatal sepsis diagnosis.The serum levels of nCD64, CRP, PCT and WBC were detected in 60 patients with neonatal sepsis and 60 patients with non-sepsis. Sensitivity, specificity, positive and negative predictive values, receiver operating characteristic (ROC) area under the curve (AUC), and logistic regression analysis were performed to evaluate the diagnostic value of these markers on neonatal sepsis.Serum levels of nCD64, PCT, CRP and WBC were higher in the sepsis group than non-sepsis group (p<0.001). The sensitivities of nCD64, PCT, CRP and WBC at the recommended cut-off level for all infants were 79.5%, 68.2%, 38.6% and 52.3%, respectively. The best combination was nCD64 and PCT, which obtained sensitivity of 90.9%, largest AUC of 0.922, and a negative predictive value of 89.2%. However by using an optimal cut-off value, the sensitivities of all four biomarkers for the diagnosis of neonatal sepsis were increased to 95.5%. Except for WBC, the birth weight and gestational age had no effects on the diagnostic value of these serum biomarkers.nCD64 and PCT are better diagnostic biomarkers for early diagnosis of neonatal sepsis as compared to CRP. With the help of optimal cut-off value based on ROC curve and logistic regression analysis, the combination of these biomarkers could improve the sensitivity for the diagnosis of suspected late-onset neonatal sepsis based on common serum biomarkers.

Vitamin D and Vitamin-D-Binding Protein Kinetics in Patients Treated with Continuous Ambulatory Peritoneal Dialysis (CAPD)

1989 ◽  
Vol 9 (4) ◽  
pp. 281-284 ◽  
Author(s):  
Preben Joffe ◽  
James G. Heat
Keyword(s):  
Mass Transfer ◽  
Vitamin D ◽  
Peritoneal Dialysis ◽  
Continuous Ambulatory Peritoneal Dialysis ◽  
Binding Protein ◽  
Serum Levels ◽  
Vitamin D Binding Protein ◽  
Normal Range ◽  
Protein Kinetics ◽  
25 Hydroxycholecalciferol

Serum and dialysate levels of 25-hydroxycholecalciferol (25-0HD3), 1,25 dihydrox ycholecalciferol (1,25(0H)2D3), and vitamin-D-binding protein (DBP) were measured in 14 patients undergoing continuous ambulatory peritoneal dialysis (CAPD). Serum levels of 25-0HD3 and DBP were within normal range (29.1 ± 22.9 nmollL and 5.9 ± 1.1 μmol/L, respectively). Serum levels of 1,25-(0H)2D3 were subnormal in all «16 pmol/L) but one. In 5 patients, dialysate concentrations of 25-0HD3 were 2.3 ± 0.9 nmol/L, the rest had levels <1.0 nmol/L. Small quantities of 1,25-(0H)2D3 were found in the dialysate effluents. DBP could be detected in the dialysate in all patients (0.24 ± 0.06 μmol/L). Mass transfer (MT) of 25-0HD3 and DBP were respectively -10.4 ± 8.3 nmol/24 h and -1.46 ± 0.46 μmol/24 h. Peritoneal clearances of 25-0HD3 and DBP were low (0.40 ± 0.37 mL/min and 0.18 ± 0.06 mL/ min, respectively. We conclude that CAPD leads to losses of 25-0HD3 and DBP. However, the peritoneal loss of DBP is well compensated and does not result in serum deficiency. Serum 25-0HD3 levels did not correlate with time on CAPD.

scholarly journals Decreased Serum microRNA-21, microRNA-25, microRNA-146a, and microRNA-181a in Autoimmune Diabetes: Potential Biomarkers for Diagnosis and Possible Involvement in Pathogenesis

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Yiwen Liu ◽  
Minglei Ma ◽  
Jie Yu ◽  
Fan Ping ◽  
Huabing Zhang ◽  
...  
Keyword(s):  
Logistic Regression ◽  
Regression Analysis ◽  
Logistic Regression Analysis ◽  
Bioinformatics Analysis ◽  
Inflammatory Responses ◽  
Autoimmune Diabetes ◽  
Multiple Logistic Regression Analysis ◽  
Roc Curves ◽  
Serum Levels ◽  
Multiple Logistic Regression

Objective. Previous studies have revealed dysregulated circulating microRNAs (miRNAs) in patients with type 1 diabetes (T1D). Here, we explored the serum levels of miR-21, miR-25, miR-146a, and miR-181a in patients with autoimmune diabetes (T1D and latent autoimmune diabetes of adults (LADA)) compared with type 2 diabetes (T2D) and nondiabetic individuals. Design, patients, and measurements. The serum levels of miR-21, miR-25, miR-146a, and miR-181a in patients with T1D (n = 29), LADA (n = 16), and T2D (n = 31) and in nondiabetic individuals (n = 19) were determined by quantitative real-time polymerase chain reaction, and receiver-operating characteristic (ROC) curves were evaluated to determine the discriminatory performances of these four miRNAs. Furthermore, target genes and pathways potentially modulated by these four miRNAs were predicted by bioinformatics analysis to investigate the possible functions of these miRNAs in autoimmune diabetes. Subsequently, multiple logistic regression analysis was performed to identify independent predictors for autoimmune diabetes, and a nomogram was established. Results. miR-21, miR-25, miR-146a, and miR-181a were significantly downregulated in the serum of patients with autoimmune diabetes compared with those in T2D patients and nondiabetic individuals (p<0.001). The areas under the ROC curves of these four miRNAs were greater than 0.80 (p<0.001). Bioinformatics analysis suggested that miR-21, miR-25, miR-146a, and miR-181a regulated multiple genes in pathways associated with immunity, inflammatory responses, hyperglycemia, and metabolism, which are involved in the pathogenesis of autoimmune diabetes. Multiple logistic regression analysis identified miR-25 (odds ratio (OR): 0.001, p<0.05), miR-146a (OR: 0.136, p<0.05), and fasting C-peptide levels (OR: 0.064, p<0.05) as independent predictors of autoimmune diabetes. Conclusions. miR-25 and miR-146a may serve as potential circulating biomarkers and provide insights into the pathogenesis of autoimmune diabetes.

scholarly journals Comparison of serum levels of vitamin D and vitamin D-binding protein in normal, osteopenic and osteoporotic postmenopausal women

2019 ◽  
Vol 35 (2) ◽  
Author(s):  
Rafat Murad ◽  
Tabassum Mahboob ◽  
Rehana Rehman ◽  
Rozeena Baig
Keyword(s):  
Vitamin D ◽  
Postmenopausal Women ◽  
Bone Fractures ◽  
Binding Protein ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
P Value ◽  
Vitamin D Binding Protein ◽  
Mineral Density ◽  
T Score

Objective: To compare the serum levels of vitamin D, vitamin D binding protein (VDBP) calcium and phosphate in normal, osteopenic and osteoporotic postmenopausal women categorized on the basis of bone mineral density (BMD) scores. Methods: A cross sectional study carried out from May 2017 to August 2018. BMD measured by Dual energy X-ray Absorptiometry categorized women (aged 20- 70 years) into normal (n=37) (T score ≥ -1.0) osteopenic (n=25) (-2.5< T score, < -1) and osteoporotic (n= 26) (T score < -2.5) according to WHO classification. Serum concentrations of vitamin D, VDBP, calcium, phosphate analyzed by enzyme linked immunosorbent assay were compared by Analysis of Variance Results: In normal females higher levels of vitamin D and VDBP were observed [15.82 (8 - 69.18), 469.9 (269.57 - 875.55)] vs. osteopenic [(7.45 (4.66 - 15.1), 296.05 (232.58 - 420.23)] and osteoporotic women [(7.25 (3.97 - 17.49), 272.94 (202.23 - 351.24)]; [median interquartile range]; p value < 0.0001. Conclusion: Vitamin D and VDBP are linked with bone health and estimation of VDBP appears to be a valuable tool for the assessment of increased bone loss and possible risks of bone fractures especially in postmenopausal women. How to cite this:Murad R, Mahboob T, Rehman R, Baig R. Comparison of serum levels of vitamin D and vitamin D-binding protein in normal, osteopenic and osteoporotic postmenopausal women. Pak J Med Sci. 2019;35(2):---------. doi: https://doi.org/10.12669/pjms.35.2.714 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

scholarly journals The variation in free 25-hydroxy vitamin D and vitamin D-binding protein with season and vitamin D status

2017 ◽  
Vol 6 (2) ◽  
pp. 111-120 ◽  
Author(s):  
Göran Oleröd ◽  
Lillemor Mattsson Hultén ◽  
Ola Hammarsten ◽  
Eva Klingberg
Keyword(s):  
Vitamin D ◽  
Binding Capacity ◽  
Binding Protein ◽  
Serum Levels ◽  
Total Serum ◽  
Vitamin D Binding Protein ◽  
Northern Latitudes ◽  
25 Hydroxy Vitamin D ◽  
Highly Correlated ◽  
Free Serum

Purpose Serum 25-hydroxy vitamin D [25(OH)D] varies greatly with season at northern latitudes. The purpose of this study was to determine if the seasonal variations in serum total 25(OH)D are followed by a concomitant variation in free 25(OH)D or if the variation is damped by alterations in the binding capacity of DBP. Methods Serum was collected from 540 healthy blood donors (60% men; mean age 41 ± 13 years) during 12 months and analyzed for total 25(OH)D, directly measured free 25(OH)D, vitamin D-binding protein (DBP) and albumin. Calculated free 25(OH)D was estimated. Results The UV-B radiation during the sampling month was positively correlated with the serum levels of total 25(OH)D (r = 0.355, P < 0.001), directly measured free (r = 0.336, P < 0.001) and calculated free 25(OH)D (r = 0.275, P < 0.001), but not with DBP and albumin. The percentage of free 25(OH)D was higher during the winter months than that during the summer months (0.020 ± 0.005% vs 0.019 ± 0.004%; P = 0.007) and higher in participants with a serum 25(OH)D below 25 nmol/L than that in participants with a serum 25(OH)D above 75 nmol/L (0.031 ± 0.007% vs 0.017 ± 0.003%; P < 0.001). iPTH was correlated with directly measured free 25(OH)D (r = −0.226; P < 0.001), but only weakly with calculated free 25(OH)D (r = −0.095; P = 0.027). Conclusions Directly measured free serum 25(OH)D was highly correlated with total serum 25(OH)D and followed the same seasonal variation, whereas the serum concentrations of DBP and albumin were stable. The fluctuation in free 25(OH)D was only marginally damped with an increase in the percentage of free 25(OH)D during the winter months and in participants with vitamin D deficiency.

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