scholarly journals Comparative bacteriological analysis of frozen and fresh chicken meats sold around old site Bayero University Kano

2021 ◽  
Vol 14 (1) ◽  
pp. 258-262
Author(s):  
Habibu Usman Abdu ◽  
Aisha Mustapha Abubakar
Keyword(s):  
Staphylococcus Aureus ◽  
Bacterial Species ◽  
Bacterial Count ◽  
Chicken Meat ◽  
Salmonella Spp ◽  
E Coli ◽  
Meat Samples ◽  
And Staphylococcus Aureus ◽  
Klebsiella Spp ◽  
Campylobacter Spp

Foodborne pathogens are serious public healthproblems that greatly affect the cost of food production and healthcare while poultry are often associated with foodborne disease outbreaks. This study aimed at investigating the occurrence and distribution of bacterial pathogens associated with chicken meats sold around Bayero University, Kano old site. A total of twenty (20) meat samples comprised of 10 frozen and ten freshly slaughtered chicken were purchased from different selling outlets around the study area. The samples were processed using standard cultural and biochemical procedures. The results of the study showed the mean aerobic mesophilic bacterial count of 5.93 x 106 CFU/g and 3.12 x 106  CFU/g for frozen and freshly slaughtered chicken meat samples respectively. The coliform counts were 420.1 and 347.7 CFU/100g for frozen and freshly slaughtered chicken meat samples respectively. The distribution of different bacterial species revealed E. coli (26.0%), Klebsiella spp (4.0%), Salmonella spp (19.5%), Campylobacter spp. (14.6%) and Staphylococcus aureus (30%) in frozen chicken meat samples while freshly slaughtered chicken meat samples revealed E. coli (21.0%), Klebsiella spp (8.9%), Salmonella spp (30%), Campylobacter spp. (15.0%) and Staphylococcus aureus (24.0%). This showed that frozen chicken meats were more contaminated than freshly slaughtered chicken meats which might be associated with poor handling and storage conditions.

scholarly journals Antibiotic Resistance in Escherichia coli and Staphylococcus aureus from Retail Chicken Meat in Surabaya, Indonesia

2020 ◽  
Vol 3 (2) ◽  
pp. 80
Author(s):  
Annisa Aulia Maghfirani Sudarmadi ◽  
Subur Prajitno ◽  
Agung Dwi Wahyu Widodo
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Growth Promotion ◽  
Infectious Agent ◽  
Sampling Technique ◽  
Chicken Meat ◽  
Meat Sample ◽  
Meat Samples ◽  
And Staphylococcus Aureus

Introduction: Antimicrobial resistance is becoming a problem in public health. Zoonotic food-borne bacteria is infectious agent that can be transferred from animal to human through the food-producing animal we consume. Nowadays, antibiotic used for human and animal is not only to cure infection but also to aim animal’s growth promotion. It is known as non therapeutic antimicrobial agent (NTA) leading to antibiotic resistance. The third generation cephalosporins, cefotaxime, and also cefoxitin are included as important antibiotic for human. This study aims to identify the presence of cefotaxime-resistant Escherichia coli and cefoxitin-resistant Staphylococcus aureus isolated from chicken meat of both traditional and modern market in Surabaya.Methods: This is descriptive post test only experimental research. We used 8 samples of chicken meat from 4 different market using purposive sampling technique. We cultured Escherichia coli and Staphylococcus aureus from the chicken meat. Sensitivity test was done using Kirby-bauer disk-diffusion method.Results: All chicken meat sample bought from traditional market in Surabaya are contaminated by cefotaxime-sensitive Escherichia coli (n=4/4) while chicken meat sample bought from modern market are not contaminated by Escherichia coli (n=0/4). All chicken meat sample bought from traditional (n=4/4) are also contaminated by cefoxitin-sensitive Staphylococcus aureus. Half of chicken meat sample bought from modern market (n=2/4) are contaminated by cefoxitin-sensitive Staphylococcus aureus, while the other half (n=2/4) are contaminated by cefoxitin-resistant Staphylococcus aureus.Conclusion: Antibiotic resistance is found and all chicken meat samples have been highly contaminated with bacteria therefore food-processing should be done correctly.

scholarly journals Microbiological analysis and antibacterial activity of pear samples

2016 ◽  
Vol 5 (1) ◽  
pp. 1-4 ◽  
Author(s):  
Habiba Afroz ◽  
Tasnia Ahmed ◽  
Md Aftab Uddin
Keyword(s):  
Fungal Growth ◽  
Bacterial Count ◽  
Total Coliform ◽  
Microbiological Analysis ◽  
Salmonella Spp ◽  
Pseudomonas Spp ◽  
Two Samples ◽  
And Staphylococcus Aureus ◽  
Klebsiella Spp ◽  
Vibrio Spp

Present study attempted to isolate and quantify the microorganisms from the pear samples collected within Dhaka metropolis. Out of the five samples studied, total viable bacterial count and the total coliform count were near about 105 cfu/g and 104 cfu/g, respectively. Among the specific pathogens, prevalence of Salmonella spp. and Vibrio spp. were found only in two samples. Whereas Klebsiella spp, Pseudomonas spp. and Staphylococcus aureus were detected in all the samples studied. Fungal growth was also observed in each sample within the range of 8.8×104 – 9.5×104 cfu/g. The study of antibiogram revealed that, most of the pathogens were resistant to more than one antibiotic. Interestingly the pear samples showed no anti-bacterial activity against any of the laboratory isolates such as, Escherichia coli, Pseudomonas spp., Salmonella spp. and Bacillus spp.Stamford Journal of Microbiology, Vol.5(1) 2015: 1-4

scholarly journals Prevalence of Escherichia Coli, Salmonella Sp, Staphylococcus Aureus Bacteria in Chicken Meat of Traditional Market Surabaya City

2021 ◽  
Vol 8 (2) ◽  
pp. 193-204
Author(s):  
Ulfa Dwi Karisma ◽  
Nurul Wiqoyah ◽  
Suhintam Pusarawati
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Chicken Meat ◽  
Plate Count ◽  
Test Results ◽  
E Coli ◽  
Traditional Markets ◽  
Two Samples ◽  
Contaminated Food ◽  
And Staphylococcus Aureus

Foodborne disease is a disease caused by contaminated food. Chicken meat is very susceptible to contamination by bacteria. Escherichia coli, Salmonella sp, and Staphylococcus aureus are types of bacteria found in chicken meat. The purpose of this study was to see how many Escherichia coli, Salmonella sp, and Staphylococcus aureus colonies in raw chicken meat in traditional markets in Surabaya City. The research sample used was 30 samples of chicken meat taken in 5 traditional markets in Surabaya. Six chicken meat sellers were taken with 1 sample and put in the sterile tube from each market. The test was carried out using the Total Plate Count (TPC) method. The TPC test results showed that all samples were contaminated with E. coli, with the most colony being 2.2X10-7 CFU/g while the minor colony was 5X10-4 CFU/g. There are two samples contaminated with Salmonella sp, in the G market and the W market, with code D6 4.7X10-6 CFU/g and code E5 1X10-6 CFU/g. Meanwhile, S. aureus was only found in the W market with sample codes E1 & E6, and each sample had a colony of 2.7X 10-7 CFU/g and 2X10-5 CFU/g.

scholarly journals تقدير المحتوى الميكروبي للحوم الدجاج المحلي والمستورد في اسواق مدينة بنغازي، ليبيا

2019 ◽  
Vol 34 (3) ◽  
pp. 206-217
Author(s):  
بكار علي حاج سعيد ◽  
عادل محمد ميلاد اشلاك ◽  
عبد الله محمد عبد الله منصور
Keyword(s):  
Staphylococcus Aureus ◽  
Salmonella Spp ◽  
E Coli ◽  
Klebsiella Spp

استهدفت الدراسة تقييم النوعية البكتيرية للحم الدجاج المسوق في 5 مناطق من مدينة بنغازي خلال موسم الصيف من خلال تقدير أعداد بعض أدلة التلوث، والمتضمنة العدد الكلي للبكتيريا الهوائية وبكتيريا القولون وبكتيريا المكورات العنقودية الذهبية (Staphylococcus aureus) كما شملت الدراسة تشخيص وعزل بكتيريا السالمونيلا (Salmonella). أظهرت نتائج هذه الدراسة أن أعداد البكتيريا الهوائية الكلية في المناطق الخمسة المشمولة بالدراسة للدجاج المحلي كان بين 3.84 و 7.22 وبمتوسط 6.02 لو10 و.ت.م/جم وفي الدجاج المستورد بين 4.49 و 6.73 ومتوسط 5.94 لو10 و.ت.م/جم ، أما بكتيريا القولون فبلغت في المحلي بين 3.5 و 6.80 وبمتوسط 5.53  لو10 و.ت.م/جم وفي المستورد بين 3.0 و 6.80 وبمتوسط 5.5 لو10 و.ت.م/جم وبلغت أعداد المكورات العنقودية في المحلي في المناطق الخمسة ما بين 4.5 و 7.3 وفي المستورد بين 6.03 و 6.50 في المستورد وبمتوسط 6.40 و 6.28  لو10 و.ت.م/جم على التوالي. أجريت على العزلات اختبارات كيموحيوية للتعرف على بعض الأجناس البكتيرية الموجودة على ذبائح لحم الدجاج حيث أُخذت 22 عزلة بكتيرية شُخصت لأنواع  E. coli 40.9% و Citrobacter spp. 18.2% و Klebsiella spp. 13.6% و Salmonella spp 9.1% وPseudomonas spp. 18.2%. تشير نتائج هذه الدراسة إلى مستوى تلوث مرتفع لذبائح الدجاج المحلي مع وجود للبكتيريا الممرضة، الأمر الذي يعكس ظروفاً صحية منخفضة لهذه اللحوم ويرجع ذلك إلى عدم اتباع إجراءات صحية جيدة خلال تحضير ذبائح الدجاج وخزن وعرض الذبائح عند البيع.

Incidence of Staphylococcus aureus and Escherichia coli in Ground Beef, Broilers and Processed Meats in Pretoria, South Africa

1994 ◽  
Vol 57 (4) ◽  
pp. 305-310 ◽  
Author(s):  
S. M. VORSTER ◽  
R. P. GREEBE ◽  
G. L. NORTJÉ
Keyword(s):  
Escherichia Coli ◽  
South Africa ◽  
Staphylococcus Aureus ◽  
Ground Beef ◽  
Processed Meat ◽  
Processed Meats ◽  
E Coli ◽  
Plate Counts ◽  
Meat Samples ◽  
And Staphylococcus Aureus

Three types of processed meats (vienna sausages, shoulder ham, and cervelat), ground beef and broilers were purchased from 17 different supermarkets in the Pretoria area (South Africa) during 1991. The 232 samples were analyzed for the presence of Escherichia coli and Staphylococcus aureus, with total aerobic plate counts (APCs) also being determined. Escherichia coli was found in 74.5% of the ground beef samples, in 79.1% of the broilers, and in 27.7% of the processed meats. Staphylococcus aureus was found in 23.4% ground beef, 39.5% broiler and 7.1% processed meat samples. The total APCs ranged from as low as log10 1 CFU/g of sample (shoulder ham) to as high as log10 12.1 CFU/g (ground beef). No identifiable relationship between the total APCs and the occurrence of E. coli and/or S. aureus was evident. This study confirms the view that E. coli and S. aureus are frequent contaminants of meat, with South Africa being no exception.

scholarly journals  Antibiotic resistance of enterococci, coagulase negative staphylococci and Staphylococcus aureus isolated from chicken meat

2013 ◽  
Vol 31 (No. 1) ◽  
pp. 14-19 ◽  
Author(s):  
N.E. Yurdakul ◽  
Z. Erginkaya ◽  
E. Ünal
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Chicken Meat ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Coagulase Negative Staphylococci ◽  
Disk Diffusion Method ◽  
Enterococcus Spp ◽  
Meat Samples ◽  
And Staphylococcus Aureus

We determined the antibiotic resistance of enterococci, coagulase negative staphylococci, and Staphylococcus aureus isolated from chicken meat samples. The antibiotic resistance of the isolated strains was estimated by the Kirby-Bauer disk diffusion method (according to the NCCLS document M2-A9 suggestions). It was found that all strains of Enterococcus spp. were resistant to tetracycline, 75% of them were resistant to ciprofloxacin, and 50% of them were resistant to erythromycin, vancomycin, and chloramphenicol. Also all strains of S. aureus were resistant to tetracycline and 25% of S. aureus strains were resistant to erythromycin and chloramphenicol, whereas all strains of S. aureus were sensitive to teicoplanin and 25% of them were sensitive to vancomycin and ciprofloxacin. As for the isolate of coagulase negative staphylococci (CNS), 68.1% of them were resistant to erythromycin, 77.2% of them were resistant to tetracycline, 59% of them were resistant to vancomycin, 9% of them were resistant to teicoplanin, and 27.2% of them were resistant to both chloramphenicol and ciprofloxacin. As a result, it was found that most of the strains (all of S. aureus and Enterococcus spp., also 77.2% CNS) were resistant to tetracycline.  

Antimicrobial Resistance of Escherichia coli, Enterococci, Pseudomonas aeruginosa, and Staphylococcus aureus from Raw Fish and Seafood Imported into Switzerland

2016 ◽  
Vol 79 (7) ◽  
pp. 1240-1246 ◽  
Author(s):  
RENATE BOSS ◽  
GUDRUN OVERESCH ◽  
ANDREAS BAUMGARTNER
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Antimicrobial Agents ◽  
Bacterial Species ◽  
Health Concern ◽  
E Coli ◽  
And Staphylococcus Aureus

ABSTRACT A total of 44 samples of salmon, pangasius (shark catfish), shrimps, and oysters were tested for the presence of Escherichia coli, enterococci, Pseudomonas aeruginosa, and Staphylococcus aureus, which are indicator organisms commonly used in programs to monitor antibiotic resistance. The isolated bacterial strains, confirmed by matrix-assisted laser desorption ionization time-of-flight mass spectroscopy, were tested against a panel of 29 antimicrobial agents to obtain MICs. Across the four sample types, Enterococcus faecalis (59%) was most common, followed by E. coli (55%), P. aeruginosa (27%), and S. aureus (9%). All bacterial species were resistant to some antibiotics. The highest rates of resistance were in E. faecalis to tetracycline (16%), in E. coli to ciprofloxacin (22%), and in S. aureus to penicillin (56%). Antibiotic resistance was found among all sample types, but salmon and oysters were less burdened than were shrimps and pangasius. Multidrug-resistant (MDR) strains were exclusively found in shrimps and pangasius: 17% of pangasius samples (MDR E. coli and S. aureus) and 64% of shrimps (MDR E. coli, E. faecalis, and S. aureus). Two of these MDR E. coli isolates from shrimps (one from an organic sample) were resistant to seven antimicrobial agents. Based on these findings, E. coli in pangasius, shrimps, and oysters, E. faecalis in pangasius, shrimps, and salmon, and P. aeruginosa in pangasius and shrimps are potential candidates for programs monitoring antimicrobial resistance. Enrichment methods for the detection of MDR bacteria of special public health concern, such as methicillin-resistant S. aureus and E. coli producing extended-spectrum β-lactamases and carbapenemases, should be implemented.

scholarly journals Detection of microbial contamination in chicken meat from local markets in Surabaya, East Java, Indonesia

2021 ◽  
pp. 3138-3143
Author(s):  
Dhandy Koesoemo Wardhana ◽  
Ajeng Erika Prihastuti Haskito ◽  
Muhammad Thohawi Elziyad Purnama ◽  
Devi Ayu Safitri ◽  
Suwaibatul Annisa
Keyword(s):  
Microbial Contamination ◽  
Chicken Meat ◽  
Most Probable Number ◽  
Biochemical Tests ◽  
Salmonella Spp ◽  
Probable Number ◽  
Local Markets ◽  
E Coli ◽  
Gram Staining ◽  
Meat Samples

Background and Aim: Chicken meat can be contaminated by microorganisms anywhere in the supply chain, from farm to market, and these microorganisms can be transmitted to humans through direct contact, contact with the environment, and food consumption. The microbial contamination has a serious impact on public health. This study aimed to analyze the microbial contamination of chicken meat sampled from local markets in Surabaya, East Java, Indonesia. Materials and Methods: A total of 60 samples of fresh chicken meat obtained from 10 traditional markets (six samples per market) were examined for the presence of bacteria. Staphylococcus aureus, Salmonella spp., and Escherichia coli were identified using Gram staining, culturing, and biochemical tests. The most probable number (MPN) method was used to identify E. coli. Results: Most chicken meat samples were positive for S. aureus (58.3%), Salmonella spp. (48.3%), and E. coli (40%). The samples were considered positive for E. coli if the MPN value was higher than 1×101 CFU/g. Conclusion: High microbial contamination was found in all the chicken meat sampled from local markets in Surabaya. Such contamination can lead to foodborne diseases so, proper hygiene and sanitation standards should be followed from slaughterhouses to the end-users.

DECONTAMINATION OF VETERINARY SURVEILLANCE OBJECTS BY NEW GENERATION MEDICINE

1970 ◽  
pp. 64-67
Author(s):  
М. S. Saypullaev ◽  
А. U. Koychuev ◽  
Т. B. Mirzoeva
Keyword(s):  
Staphylococcus Aureus ◽  
Inhalation Exposure ◽  
Gram Negative Bacteria ◽  
Hazard Class ◽  
E Coli ◽  
Highly Efficient ◽  
Mucous Membranes ◽  
Environmentally Safe ◽  
New Generation ◽  
And Staphylococcus Aureus

The successful conduct of disinfection measures largely depends on the availability of veterinary practice a highly efficient, environmentally safe disinfectants. In this regard, finding new highly efficient disinfectant remains relevant. Studies found that the "Polied" (OOO "Razvitie XXI Vek, Russia) can be attributed to the highly efficient and environmentally friendly means. Solutions "Polied" have a high disinfectant activity against smooth and rough surfaces in the laboratory against gram-positive, gram-negative bacteria, mycobacteria and spores of microorganisms. Studies have established that solutions should be "Polied" obezzarajivatmi E. coli (EA 1257) concentrations of 0.1% on smooth surfaces and Staphylococcus aureus concentration of 0.05% in 1 hour from the calculation of 0.25-0.3 litres/m2. Disinfection of rough test surfaces against Escherichia coli and Staphylococcus aureus occurred after treatment with 0,3% solution of 3-hour exposure, at a rate of 0.5 l/m2. It was also found that 1.0% solution "Polied" fully obezzarazhivatel test the surface of mycobacteria (PCs-5) and at double the 0.6% concentration for 24 hours. Disinfection of rough test surfaces contaminated with spores of B. cereus (PCs 96) was achieved with a 4.0% solution at twice the irrigation rate of 0.5 l/m2 at an exposure of 24 hours. Toxicity solutions of the drug "Polied" refer to "moderate" threat (hazard class 3) and low-hazard substances (4 hazard class) when applied to the skin, mucous membranes of the eyes, and inhalation exposure on the respiratory system.

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