Assessment of copper accumulation in archived liver specimens from cats

2020 ◽  
pp. 1098612X2096135
Author(s):  
Punyamanee Yamkate ◽  
Randi M Gold ◽  
Panagiotis G Xenoulis ◽  
Katja Steiger ◽  
David C Twedt ◽  
...  
Keyword(s):  
Infectious Disease ◽  
Hepatic Steatosis ◽  
Dry Weight ◽  
Reference Interval ◽  
Copper Accumulation ◽  
Zonal Distribution ◽  
Tissue Samples ◽  
Hepatic Copper ◽  
Liver Copper ◽  
Flame Atomic Absorption

Objectives The aim of this study was to assess hepatic copper concentrations and zonal distribution in cat liver specimens. Methods For this study, 121 archived, formalin-fixed, paraffin-embedded liver specimens from cats were used. Tissue sections were stained for copper with rhodanine and scored from 0 (no copper accumulation) to 5 (panlobular copper accumulation). The tissue specimens were then deparaffinized and hepatic copper concentrations were measured using flame atomic absorption spectroscopy. Results Tissue samples were categorized into four groups based on histopathologic findings: (1) no significant histopathologic hepatic changes (n = 66); (2) hepatic steatosis (n = 18); (3) inflammatory or infectious disease (n = 24); and (4) neoplasia (n = 13). Of the 121 specimens, 13 (11%) stained positive for copper, with three having a score ⩾3. Thirty-seven specimens (31%) had copper concentrations above the reference interval ([RI] <180 µg/g dry weight liver). Copper concentrations in cats with hepatic inflammatory or infectious disease were significantly higher than cats with hepatic steatosis ( P = 0.03). Copper-staining score and concentration were positively correlated ( rs = 0.46, P <0.001). Conclusions and relevance Despite the fact that 31% of specimens had copper concentrations above the RI, only 11% showed positive copper staining and only 2.5% had a score ⩾3. Our findings suggest that hepatic copper concentrations greater than the upper limit of the RI are relatively common in cats. Further studies to determine the factors that influence hepatic copper staining in cats and to establish contemporary RIs for hepatic copper in healthy cats are warranted.

Histomorphological and Immunohistochemical Studies of Chronic Active Hepatitis in Doberman Pinschers

1998 ◽  
Vol 35 (5) ◽  
pp. 380-385 ◽  
Author(s):  
L. P. Thornburg
Keyword(s):  
Hepatic Vein ◽  
Chronic Active Hepatitis ◽  
Plasma Cells ◽  
Normal Liver ◽  
Scar Tissue ◽  
Hepatic Veins ◽  
Tissue Samples ◽  
Hepatic Copper ◽  
Liver Copper ◽  
Doberman Pinschers

Liver tissue samples were reviewed from 35 Doberman Pinschers with chronic active hepatitis in the precirrhotic stage. Thirty dogs had elevated hepatic copper concentrations, and five had normal liver copper concentrations. The earliest changes were inflammation and scar tissue deposition around the small hepatic vein branches. There was also apoptosis of scattered hepatocytes in zone 3. Inflammation consisted of macrophages, lymphocytes, and plasma cells. As the disease progressed, collagen deposition increased around the hepatic veins; in some liver specimens, thin scar tissue septa radiated from the hepatic vein branches, and inflammation spread to include the portal tracts. The sinusoids adjacent to the scar tissue were converted to endothelial-lined, thin-walled vessels. Chronic active hepatitis (commonly referred to as Doberman hepatitis or chronic active hepatitis of Dobermans) is a progressive fibrosis, inflammation and hepatocyte loss beginning among zone 3 hepatocytes around the terminal hepatic vein branches. The histomorphologic changes were the same among those Dobermans with elevated hepatic copper and those with normal hepatic copper. The cause was not determined, but these morphologic studies support the idea of immune-mediated disease.

scholarly journals Evaluation of a commercially available molybdate formulation and zinc oxide boluses in preventing hepatic copper accumulation and thus enzootic icterus in sheep

2001 ◽  
Vol 72 (4) ◽  
Author(s):  
C.J. Botha ◽  
A.S. Shakespeare ◽  
R. Gehring ◽  
D. Van der Merwe
Keyword(s):  
Zinc Oxide ◽  
Copper Concentration ◽  
Histopathological Examination ◽  
Copper Accumulation ◽  
Copper Excretion ◽  
Hepatic Copper ◽  
Liver Copper ◽  
Urinary Copper ◽  
Plasma Copper ◽  
Urinary Copper Excretion

The efficacy of a molybdate formulation and a zinc oxide bolus as prophylactic agents for enzootic icterus was evaluated in sheep. Before copper loading, liver biopsies were performed on 12 male, 6-month-old, Mutton Merino sheep to determine hepatic copper (Cu) and zinc (Zn) concentrations. The animals were restrictively randomised according to liver copper concentrations to 3 treatment groups (n = 4) to achieve similar mean liver copper concentrations per group. All sheep received 4 m /kg of a 0.5 %aqueous solution of CuSO4·5H2O intraruminally 7 days per week for 10 weeks. On Day 0 the sheep in the Mo-group were injected subcutaneously with 42 mg molybdenum (Mo) contained in a commercial molybdate formulation. The animals in the Zn-group each received a zinc oxide bolus, containing 43 g zinc oxide, via a rumen cannula. Treatment was repeated on Day 42. Four animals served as untreated controls. Urinary copper excretion, plasma copper concentration, haematocrit and glutamate dehydrogenase (GLDH) activity were determined throughout the trial. The animals were sacrificed after 10 weeks and liver samples were submitted for histopathological examination. Liver and kidney copper and zinc concentrations were determined. Neither the molybdate treatment nor the zinc oxide boluses prevented hepatic copper accumulation. The urinary copper excretion, plasma copper concentration, haematocrit and GLDH activity were not significantly different (P > 0.05) from the controls.

Copper-Associated Hepatitis in Labrador Retrievers

2009 ◽  
Vol 46 (3) ◽  
pp. 484-490 ◽  
Author(s):  
R. Smedley ◽  
T. Mullaney ◽  
W. Rumbeiha
Keyword(s):  
Hepatic Cirrhosis ◽  
Animal Health ◽  
Dry Weight ◽  
Copper Level ◽  
Michigan State University ◽  
State University ◽  
Hepatic Copper ◽  
Liver Copper ◽  
Diagnostic Center ◽  
Labrador Retrievers

Labrador Retrievers with elevated hepatic copper levels have been reported; however, it is unclear whether primary copper-associated hepatitis occurs in this breed. The objective of this study was to determine whether copper-associated hepatitis could be identified in Labradors by reviewing cases from the Diagnostic Center for Population and Animal Health, Michigan State University. Sixteen Labrador Retrievers (3 male, 12 female, and 1 of undetermined sex) between 4 and 11 years old, had multifocal and coalescing, centrilobular hepatitis characterized by macrophages with abundant intracytoplasmic copper and hemosiderin. Other lesions included multifocal, centrilobular and random, pigmented granulomas, hepatocellular necrosis, intrahepatic cholestasis, centrilobular or bridging fibrosis, and occasionally, pseudolobule formation. In rhodanine-stained sections, copper was concentrated in the cytoplasm of centrilobular and midzonal hepatocytes and in macrophages, which is consistent with copper-associated hepatitis. In 12 of the dogs, quantitative liver copper levels were available, and in all but 2 dogs the levels were greater than 2,000 parts per million dry weight (ppm dw). One dog had a liver copper level of 1,990 ppm dw and one dog with advanced hepatic cirrhosis had a level of 1,490 ppm dw. The findings suggest that primary copper-associated hepatitis likely occurs in Labrador Retrievers.

scholarly journals Interannual and Spatial Variability of Cyanotoxins in the Prespa Lake Area, Greece

Water ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 357
Author(s):  
Valentini Maliaka ◽  
Miquel Lürling ◽  
Christian Fritz ◽  
Yvon J.M. Verstijnen ◽  
Elisabeth J. Faassen ◽  
...  
Keyword(s):  
Water Quality ◽  
Spatial Variability ◽  
Spatial Heterogeneity ◽  
Dry Weight ◽  
Ecosystem Functions ◽  
Lake Area ◽  
Total Chlorophyll ◽  
Tissue Samples ◽  
Cyanobacteria Blooms ◽  
Prespa Lake

The Prespa Lakes area in Greece—comprised partly of lake Great and lake Lesser Prespa and the Vromolimni pond—has a global importance for biodiversity. Although the waters show regular cyanobacteria blooms, assessments of water quality threats are limited. Samples collected in 2012 revealed scattered and low microcystin (MC) concentrations in Great Prespa (<0.2 μg MC L−1) whereas considerable spatial heterogeneity in both total chlorophyll (2.4–93 µg L−1) and MC concentrations (0.04–52.4 µg MC L−1) was detected in Lesser Prespa. In 2013, there was far less spatial variability of MC concentrations in Lesser Prespa (0.4–1.53 µg L−1), however in 2014, increased concentrations were detected near the lakeshore (25–861 µg MC L−1). In Vromolimni pond the MC concentrations were on average 26.6 (±6.4) µg MC L−1 in 2012, 2.1 (±0.3) µg MC L−1 in 2013 and 12.7 (±12.5) µg MC L−1 in 2014. In 2013, no anatoxins, saxitoxins, nor cylindrospermopsins were detected in Lesser Prespa and Vromolimni waters. Tissue samples from carps, an otter and Dalmatian Pelicans contained 0.4–1.9 µg MC g−1 dry weight. These results indicate that cyanotoxins could be a threat to the ecosystem functions of particularly Lesser Prespa and Vromolimni.

Hepatic Copper Accumulation Induces DNA Strand Breaks in the Liver Cells of Long-Evans Cinnamon Strain Rats

2000 ◽  
Vol 276 (1) ◽  
pp. 174-178 ◽  
Author(s):  
Masanobu Hayashi ◽  
Tomoko Kuge ◽  
Daiji Endoh ◽  
Kenji Nakayama ◽  
Jiro Arikawa ◽  
...  
Keyword(s):  
Dna Strand Breaks ◽  
Liver Cells ◽  
Copper Accumulation ◽  
Strand Breaks ◽  
Hepatic Copper ◽  
Long Evans ◽  
Dna Strand

scholarly journals An 112 Days Experiment on Dietary Cadmium Retention in Hepatopancreas in Adult Cantareus aspersus Snails

2019 ◽  
Vol 70 (8) ◽  
pp. 2803-2804
Author(s):  
George A. Draghici ◽  
Cristina A. Dehelean ◽  
Iulia Pinzaru ◽  
Despina M. Bordean ◽  
Georgeta Pop ◽  
...  
Keyword(s):  
Absorption Spectrometry ◽  
Dry Weight ◽  
Land Snails ◽  
Dependent Manner ◽  
Flame Atomic Absorption ◽  
Wide Range ◽  
Cantareus Aspersus ◽  
Ecotoxicological Model ◽  
Dietary Cadmium ◽  
Kinetics Of

Cadmium is very hazardous pollutant with massive impact on aspects of everyday life. Little knowledge exists on kinetics of dietary cadmium retention in Cantareus aspersus for durations above three months although this species of land snails serves as excellent ecotoxicological model for studying cadmium hazard. Here was used a continuous 112-day exposure study design, in which test snails were exposed to a wide range of dietary cadmium levels, including human- and environmentally-relevant levels. Using Flame Atomic Absorption Spectrometry, cadmium levels in the hepatopancreas were found to increase significantly, and in dose-dependent manner starting from a dietary cadmium dose of 0.2 mg/kg dry weight. The results of the present study render the hepatopancreas of mature snails, C. aspersus, as excellent endpoints for assessing Cd toxicity over a broad range of concentrations. Hence, this species of land snails can be reliably used for both active and passive biomonitoring of environmental cadmium pollution.

scholarly journals Human Activity in Antarctica: Effects on Metallic Trace Elements (MTEs) in Plants and Soils

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2593
Author(s):  
Jaime Tapia ◽  
Marco Molina-Montenegro ◽  
Camila Sandoval ◽  
Natalia Rivas ◽  
Jessica Espinoza ◽  
...  
Keyword(s):  
Human Activity ◽  
Early Stage ◽  
Vascular Plant ◽  
Tissue Samples ◽  
Flame Atomic Absorption Spectroscopy ◽  
Flame Atomic Absorption ◽  
Sampling Locations ◽  
Metallic Trace Elements ◽  
Metallic Trace Element ◽  
The Antarctic

Colobanthus quitensis (Kunt) is one of the two vascular plant species present in Antarctica and develops under severe environmental conditions, being found in both pristine and human-threatened environments. We determined the Cd, Cr, Cu, Mn, Ni, Pb, and Zn levels in C. quitensis roots, leaves, and soils of origin using flame atomic absorption spectroscopy. In January 2017, we collected samples from four geographical zones on the longitudinal gradient along which C. quitensis is distributed, starting from Punta Arenas (PAR) at the extreme south of mainland Chile and moving southwards to the Antarctic territory from King George Island (KGI) to Hannah Point Peninsula (PHA) and finally Lagotellerie Island (LAT). We used certified reference material to validate the plant tissues and soil samples we collected. The highest concentrations of metals that we measured in the soils and in the C. quitensis roots and leaves were in samples we collected at the KGI station, the zone with the greatest human activity. The lowest concentrations we measured were at the LAT station, an island with little human intervention and scarce fauna. The mean concentrations of metals in the roots and leaves of C. quitensis followed a similar order at all sampling locations: Mn > Zn > Cu > Ni > Pb > Cr > Cd. In contrast, in soil, they followed the following order: Mn > Zn > Cu > Cr > Pb > Ni > Cd. The concentration levels obtained for the different metals in the soil and plants tissue samples in this region of Antarctica indicated that the area was non-polluted. However, the metallic trace element (MTE) concentrations may be at an early stage of contamination, as described in other areas of the Antarctic, being a new threat to this continent.

Micro-quantity tissue digestion for metal measurements by use of a microwave acid-digestion bomb.

1989 ◽  
Vol 35 (3) ◽  
pp. 488-490 ◽  
Author(s):  
J R Nicholson ◽  
M G Savory ◽  
J Savory ◽  
M R Wills
Keyword(s):  
Nitric Acid ◽  
Reference Material ◽  
Microwave Digestion ◽  
Absorption Spectrometry ◽  
Bovine Liver ◽  
Dry Weight ◽  
Acid Digestion ◽  
Tissue Samples ◽  
Dry Ashing ◽  
Tissue Digestion

Abstract We describe a simple and convenient method for processing small amounts of tissue samples for trace-metal measurements by atomic absorption spectrometry, by use of a modified Parr microwave digestion bomb. Digestion proceeds rapidly (less than or equal to 90 s) in a sealed Teflon-lined vessel that eliminates contamination or loss from volatilization. Small quantities of tissue (5-100 mg dry weight) are digested in high-purity nitric acid, yielding concentrations of analyte that can be measured directly without further sample manipulation. We analyzed National Institute of Standards and Technology bovine liver Standard Reference Material to verify the accuracy of the technique. We assessed the applicability of the technique to analysis for aluminum in bone by comparison with a dry ashing procedure.

Hydride Generation and Condensation Flame Atomic Absorption Spectroscopic Determination of Antimony in Raw Coffee Beans and Processed Coffee

1982 ◽  
Vol 65 (5) ◽  
pp. 1146-1149
Author(s):  
Roy W Kuennen ◽  
Mark H Hahn ◽  
Fred L Fricke ◽  
Karen A Wolnik
Keyword(s):  
Atomic Absorption ◽  
Inductively Coupled Plasma ◽  
Hydride Generation ◽  
Dry Weight ◽  
Atomic Emission ◽  
Standard Reference Materials ◽  
National Bureau ◽  
Total Acid ◽  
Flame Atomic Absorption ◽  
Coffee Beans

Abstract A method was developed for determining Sb at nanogram per gram levels in raw coffee beans and processed coffee. The procedure uses either total acid digestion or extraction with 6M HCl followed by hydride generation/condensation with subsequent revolatilization of stibine (SbH3) and detection by flame atomic absorption spectroscopy. The lowest quantifiable level, based on a 2 g (dry weight) sample, is 2 ng Sb/g. The results of recoveries on spiked samples, precision studies on composited coffee samples, and the analysis of National Bureau of Standards Standard Reference Materials demonstrate the reliability and accuracy of the procedure. Sb concentrations in coffee samples were verified by neutron activation analysis and inductively coupled plasma atomic emission spectroscopy. Advantages of the method compared with the AOAC colorimetric procedure and hydride generation without condensation are discussed.

scholarly journals Regulation of murine copper homeostasis by members of the COMMD protein family

2020 ◽  
Vol 14 (1) ◽  
pp. dmm045963
Author(s):  
Amika Singla ◽  
Qing Chen ◽  
Kohei Suzuki ◽  
Jie Song ◽  
Alina Fedoseienko ◽  
...  
Keyword(s):  
Copper Homeostasis ◽  
Copper Accumulation ◽  
Copper Transporter ◽  
Copper Excretion ◽  
Copper Absorption ◽  
Hepatic Copper ◽  
High Copper ◽  
Molecular Machinery ◽  
And Function

ABSTRACTCopper is an essential transition metal for all eukaryotes. In mammals, intestinal copper absorption is mediated by the ATP7A copper transporter, whereas copper excretion occurs predominantly through the biliary route and is mediated by the paralog ATP7B. Both transporters have been shown to be recycled actively between the endosomal network and the plasma membrane by a molecular machinery known as the COMMD/CCDC22/CCDC93 or CCC complex. In fact, mutations in COMMD1 can lead to impaired biliary copper excretion and liver pathology in dogs and in mice with liver-specific Commd1 deficiency, recapitulating aspects of this phenotype. Nonetheless, the role of the CCC complex in intestinal copper absorption in vivo has not been studied, and the potential redundancy of various COMMD family members has not been tested. In this study, we examined copper homeostasis in enterocyte-specific and hepatocyte-specific COMMD gene-deficient mice. We found that, in contrast to effects in cell lines in culture, COMMD protein deficiency induced minimal changes in ATP7A in enterocytes and did not lead to altered copper levels under low- or high-copper diets, suggesting that regulation of ATP7A in enterocytes is not of physiological consequence. By contrast, deficiency of any of three COMMD genes (Commd1, Commd6 or Commd9) resulted in hepatic copper accumulation under high-copper diets. We found that each of these deficiencies caused destabilization of the entire CCC complex and suggest that this might explain their shared phenotype. Overall, we conclude that the CCC complex plays an important role in ATP7B endosomal recycling and function.

Sign in / Sign up

Export Citation Format

Share Document