scholarly journals Speculative feminism and the shifting frontiers of bioscience: envisioning reproductive futures with synthetic gametes through the ethnographic method

2021 ◽  
pp. 146470012110301
Author(s):  
Mianna Meskus
Keyword(s):  
Science Fiction ◽  
Science Studies ◽  
Thought Experiments ◽  
Sperm Cells ◽  
Cultural Products ◽  
Political Issues ◽  
Feminist Analysis ◽  
Stratified Reproduction ◽  
Ethnographic Methodology

Scientists are developing a technique called in vitro gametogenesis or IVG to generate synthetic gametes for research and, potentially, for treating infertility. What would it mean for feminist concerns over the future of reproductive practice and biotechnological development if egg and sperm cells could be produced in laboratory conditions? In this article, I take on the question by discussing the emerging technique of IVG through the speculative feminist analysis of ambiguous reproductive futures. Feminist cultural and science studies scholars have explored the transformative effects of biomedicine on reproduction through science fiction novels and other cultural products. I theorise the speculative and visionary in biomedicine in the context of ethnographic methodology by drawing on ‘thought experiments’ conducted with stem cell scientists as shared acts of future-oriented contemplation. I develop the figure of SF proposed by Donna Haraway to investigate how science facts and speculative fabulation together shape futurities of reproduction. I propose including shifting frontiers in feminist thinking of the SFs in bioscience. Biomedical research aims to shift the borders between what is known and not known in reproductive biology, subsequently raising new technical, ethical and political issues in terms of stratified reproduction. The article shows that synthetic gametes are anticipated to intensify selective procreation. Simultaneously, IVG is seen to forge new biogenetic relationships and possibilities for non-normative reproduction and kin-making. Following Haraway, I argue that by ‘staying with the trouble’ of these biotechnological visions, feminist speculative analytics on technoscience offers a valuable tool to envision more hopeful and equal futures together with scientists.

Faeries, Aliens, and Leviathans: Science and Fantasy in Ian McDonald's King of Morning, Queen of Day

2019 ◽  
Vol 49 (2) ◽  
pp. 290-303
Author(s):  
Richard Howard
Keyword(s):  
Twentieth Century ◽  
National Identity ◽  
Science Fiction ◽  
Science Studies ◽  
Irish Studies ◽  
Early Twentieth Century ◽  
Politics And Religion ◽  
Unexplored Area ◽  
Late Nineteenth ◽  
Cultural Movement

Irish science fiction is a relatively unexplored area for Irish Studies, a situation partially rectified by the publication of Jack Fennell's Irish Science Fiction in 2014. This article aims to continue the conversation begun by Fennell's intervention by analysing the work of Belfast science fiction author Ian McDonald, in particular King of Morning, Queen of Day (1991), the first novel in what McDonald calls his Irish trilogy. The article explores how McDonald's text interrogates the intersection between science, politics, and religion, as well as the cultural movement that was informing a growing sense of a continuous Irish national identity. It draws from the discipline of Science Studies, in particular the work of Nicholas Whyte, who writes of the ways in which science and colonialism interacted in late nineteenth and early twentieth-century Ireland.

Mechanism of the reduction in DNA content in sperm cells preserved in vitro

1969 ◽  
Vol 67 (4) ◽  
pp. 383-385 ◽  
Author(s):  
I. I. Ivanov ◽  
N. V. Korban ◽  
V. I. Sharobaiko
Keyword(s):  
Dna Content ◽  
Sperm Cells

scholarly journals Cooling of equine semen at 16°C for 36 hours with addition of different glutathione concentrations

2015 ◽  
Vol 36 (6) ◽  
pp. 3699
Author(s):  
Rodrigo Arruda de Oliveira ◽  
Marco Antônio De Oliveira Viu ◽  
Maria Lúcia Gambarini
Keyword(s):  
Lipid Peroxidation ◽  
Sperm Motility ◽  
Membrane Integrity ◽  
Membrane Lipid ◽  
Sperm Cells ◽  
Sperm Viability ◽  
Membrane Lipid Peroxidation ◽  
Acrosomal Membrane ◽  
In Vitro Effects

Handling equine semen during the refrigeration process reduces sperm viability, and consequently causes membrane lipid peroxidation, among other challenges. The present study aimed to evaluate the in vitro effects of glutathione (control, 1. 0, 1. 5, and 2. 5 mM) on equine semen in a refrigeration protocol of 16ºC for 36 hours. The following variables were evaluated after 0, 12, 24, and 36 hours refrigeration: total sperm motility, vigor, viability, and plasma and acrosomal membrane integrity. Motility was higher with 2. 5mM of glutathione (57. 8 ± 7. 3) after 12 hours of refrigeration compared to the control (53. 2 ± 8. 3) (P < 0. 05). After 36 hours of refrigeration, motility was higher with 1. 5 mM (43. 4 ± 12. 7) and 2. 5mM glutathione (45. 5 ± 6. 2), than it was with 1mM glutathione (38. 2 ± 9) and the control (35. 5 ± 18. 4) (P < 0. 05), respectively. Vigor was highest with 1. 5mM glutathione (3. 7 ± 0. 3) after 36 hours compared to the control (3. 2 ± 1. 1), (P < 0. 05). Viability differed between control and 1mM treatments (79. 5 ± 1. 8) only after 24 hours (75. 5 ± 9. 7) (P < 0. 05). Throughout the investigation, no significant differences were noted in plasma and acrosomal membrane integrity (P > 0. 05). The 1. 5 and 2. 5mM glutathione levels were more efficient in protecting sperm cells and yielded higher total motility values after 36 hours of refrigeration.

Chlortetracycline staining patterns of frozen-thawed bull spermatozoa treated with β-cyclodextrins, dibutyryl cAMP and progesterone

Zygote ◽  
2000 ◽  
Vol 8 (3) ◽  
pp. 245-256 ◽  
Author(s):  
Michael B. Dinkins ◽  
Benjamin G. Brackett
Keyword(s):  
Intestinal Mucosa ◽  
Sperm Cells ◽  
Fluorescent Staining ◽  
Sperm Capacitation ◽  
Dibutyryl Camp ◽  
Bovine Spermatozoa ◽  
Differential Responses ◽  
Definition Of

Efforts to achieve complete chemical definition of media used for in vitro capacitation of bovine spermatozoa including removal of heparin purified from porcine intestinal mucosa are presented. Fluorescent staining with chlortetracycline (CTC), known to reflect changes coincident with sperm capacitation in certain species, was studied following treatments of frozen-thawed bull spermatozoa with β-cyclodextrins, dibutyryl cAMP (dbcAMP) and progesterone in comparison with heparin. The CTC staining patterns (F, B and AR) were confirmed to correlate with known conditions that effectively prepare cryopreserved bull spermatozoa for fertilisation in vitro. In the absence of glucose, the routinely employed heparin-containing capacitating medium caused an increase in spermatozoa displaying the AR pattern. Both progesterone (100 μM) and dbcAMP (0.01–0.1 mM) were able to increase the proportion of B pattern stained sperm cells more than after exposure to control (mDM) conditions without a significant reduction in motility. Exposure to either dbcAMP or β-cyclodextrins was accompanied by an increase in proportions of spermatozoa displaying the AR pattern over those seen in controls. Exposure to β-cyclodextrins did not increase the proportion of B pattern stained spermatozoa. Comparison of spermatozoa from two bulls revealed differential responses of spermatozoa from different males to treatments with heparin and progesterone. In vitro fertilisation results demonstrated that previously cryopreserved bull spermatozoa could be capacitated in chemically defined conditions devoid of heparin or other biological components.

Zombies, Epiphenomenalism, and Physicalist Theories of Consciousness

2006 ◽  
Vol 36 (4) ◽  
pp. 481-509 ◽  
Author(s):  
Andrew Bailey
Keyword(s):  
Science Fiction ◽  
Computational Model ◽  
Philosophy Of Mind ◽  
Thought Experiments ◽  
Computer Programs ◽  
Recent History ◽  
Philosophical Work ◽  
The Mind

In its recent history, the philosophy of mind has come to resemble an entry into the genre of Hammer horror or pulpy science fiction. These days it is unusual to encounter a major philosophical work on the mind that is not populated with bats, homunculi, swamp-creatures, cruelly imprisoned genius scientists, aliens, cyborgs, other-worldly twins, self-aware Computer programs, Frankenstein-monster-like ‘Blockheads,’ or zombies. The purpose of this paper is to review the role in the philosophy of mind of one of these fantastic thought-experiments — the zombie — and to reassess the implications of zombie arguments, which I will suggest have been widely misinterpreted. I shall argue that zombies, far from being the enemy of materialism, are its friend; and furthermore that zombies militate against the computational model of consciousness and in favour of more biologically-rooted conceptions, and hence that zombie- considerations support a more reductive kind of physicalism about consciousness than has been in vogue in recent years.

scholarly journals Spermatozoa Obtained From Alpaca vas deferens. Effects of Seminal Plasma Added at Post-thawing

2021 ◽  
Vol 8 ◽  
Author(s):  
Eduardo G. Aisen ◽  
Wilfredo Huanca López ◽  
Manuel G. Pérez Durand ◽  
Edita Torres Mamani ◽  
Juan C. Villanueva Mori ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Artificial Insemination ◽  
Reproductive Tract ◽  
Vas Deferens ◽  
Female Reproductive Tract ◽  
Low Fertility ◽  
Sperm Cells ◽  
South American Camelids ◽  
Thawing Process

The viscous seminal plasma (SP) is currently a major impediment to the handling of ejaculate and the development of some biotechnologies in South American camelids. The vas deferens-collected spermatozoa of alpacas is a useful technique to avoid this problem. On the other hand, SP contains a large protein component that has been implicated in the function of spermatozoa within the female reproductive tract. In this sense, the low fertility achieved using transcervical insemination with frozen-thawed spermatozoa in alpacas could be improved by adding SP. This study aimed to evaluate the effect of the whole SP on some in vitro parameters of alpaca spermatozoa after the freezing-thawing-process and the fertility after artificial insemination. It would contribute to a better understanding of the interaction between thawed sperm cells and SP. Spermatozoa were obtained by surgically diverted vas deferens. The samples were diluted with a Tris-based extender, packaged in straws, and frozen. At thawing, each straw was divided into two post-thawing conditions: with the addition of 10% of PBS (control) or with 10% SP (treatment). The sperm cells were evaluated using dynamic parameters, sperm cell morphology, and morphometry. Fertility was assessed by an artificial insemination trial. All in vitro parameters were analyzed by ANOVA. A heterogeneity test was scheduled for the fertility trial. After the freezing-thawing process, motility and plasma membrane functionality was improved when SP was added. No differences were found for post-thaw viability between the control and treatment samples. The percentage of normal cells was higher with SP at post-thawing, and a decrease of the presence of bent tailed spermatozoa with a droplet in the SP group was observed. The length of the head spermatozoa was 3.4% higher in the samples with PBS compared to those in which SP was added. Females pregnant at day 25 post-insemination were 0/12 (with SP inside the straw) and 1/10 (without SP inside the straw). In conclusion, the presence of 10% SP at post-thawing improves sperm cells' motility, functionality, and morphology, indicating that it would be beneficial to improve the frozen-thawed alpaca's physiology spermatozoa. More fertility trials must be developed to increase this knowledge.

scholarly journals Identity Theft: A Thought Experiment on the Fragility of Identity

Conatus ◽  
2020 ◽  
Vol 5 (1) ◽  
pp. 71
Author(s):  
David Menčik
Keyword(s):  
Science Fiction ◽  
Personal Identity ◽  
Thought Experiment ◽  
Psychological Impact ◽  
Thought Experiments ◽  
Identity Theft ◽  
Psychological Continuity ◽  
Temporal Proximity ◽  
Shed Light ◽  
The Prestige

This paper intends to discuss some aspects of what we conceive as personal identity: what it consists in, as well as its alleged fragility. First I will try to justify the methodology used in this paper, that is, the use of allegories in ontological debates, especialy in the form of thought experiments and science fiction movies. Then I will introduce an original thought experiment I call “Who am I actually?,” one that was coined with the intent to shed light on several aspects of the issue under examination, that is, the fragility of personal identity. Then I will move on to Christopher Nolan’s film The Prestige, as well as to Derek Parfit’s ‘divided minds’ thought experiment, to further discuss the fragility of personal identity; next to identity theft, the prospect of duplication is also intriguing, especially with regard to the psychological impact this might have on both the prototype and the duplicate. I will conclude with the view that spatial and temporal proximity or coexistence, especially when paired with awareness on behalf of the duplicates, would expectedly result in the infringement of the psychological continuity of one’s identity.

scholarly journals The in vitro toxicity of atrazine on kinematics and DNA fragmentation in common carp (Cyprinus carpio) sperm cells

2019 ◽  
Vol 70 (3) ◽  
pp. 1639
Author(s):  
M.E. ÖZGÜR
Keyword(s):  
Dna Fragmentation ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
In Vitro Toxicity ◽  
Computer Assisted ◽  
Sperm Cells ◽  
Common Carp Cyprinus Carpio ◽  
Significant Difference ◽  
Carp Cyprinus Carpio

This study investigated the in vitro effects of different concentrations of Atrazine (0.001, 0.01, 0.1, 0.5 mg/L) added to motile and immotile solutions on kinematics quality of sperm cells of common carp, Cyprinus carpio, which is a fish of economic significance. The kinematics of the sperm cells was analyzed by a computer-assisted sperm analysis system (CASA). As a result of the study, while there was a significant difference (P < 0.05) between the groups in terms of the VSL (μm/s) and VCL (μm/s) values after the Atrazine-added immotile solution’s (IMS) and incubation for 3 hours, there was a significant difference (P < 0.05) in only the VSL values directly activated by the Atrazine-added motile solution (MS). DNA fragmentation was evident but not in higher numbers in the 0.1 mg/L atrazine group. Finally, it was determined the effective concentration (EC50) values of the VSL value of the motile and immotile solution as 0.34 mg/L and 0.03 mg/L, respectively.

Fighting for the Future

2020 ◽  
Keyword(s):  
Cultural Studies ◽  
Science Fiction ◽  
Political Science ◽  
Media Studies ◽  
Star Trek ◽  
Literary Studies ◽  
The Political ◽  
Television Series ◽  
Political Issues ◽  
The Moment

The first two seasons of the television series Star Trek: Discovery, the newest instalment in the long-running and influential Star Trek franchise, received media and academic attention from the moment they arrived on screen. Discovery makes several key changes to Star Trek’s well-known narrative formulae, particularly the use of more serialized storytelling, appealing to audiences’ changed viewing habits in the streaming age – and yet the storylines, in their topical nature and the broad range of socio-political issues they engage with, continue in the political vein of the franchise’s megatext. This volume brings together eighteen essays and one interview about the series, with contributions from a variety of disciplines including cultural studies, literary studies, media studies, fandom studies, history and political science. They explore representations of gender, sexuality and race, as well as topics such as shifts in storytelling and depictions of diplomacy. Examining Discovery alongside older entries into the Star Trek canon and tracing emerging continuities and changes, this volume will be an invaluable resource for all those interested in Star Trek and science fiction in the franchise era.

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