Calcium Modulates Interactions between Bacteria and Hydroxyapatite

2006 ◽  
Vol 85 (12) ◽  
pp. 1124-1128 ◽  
Author(s):  
S.C. Venegas ◽  
J.M. Palacios ◽  
M.C. Apella ◽  
P.J. Morando ◽  
M.A. Blesa
Keyword(s):  
Electrical Properties ◽  
Aqueous Medium ◽  
Surface Properties ◽  
Streptococcus Mutans ◽  
Electrophoretic Mobility ◽  
Bacterial Adhesion ◽  
Bacterial Strain ◽  
Lactobacillus Casei ◽  
Solid Surfaces ◽  
Hydrophobic Properties

Bacterial adhesion onto hydroxyapatite is known to depend on the surface properties of both the biomaterial and the bacterial strain, but less is known about the influence of the composition of the aqueous medium. Here, the adhesion of Streptococcus mutans and 3 different Lactobacilli on powdered hydroxyapatite was shown to change with Ca2+ concentration. The effect depends on the surface properties of each strain. Adhesion of Lactobacillus fermentum and salivarius (and of Streptococcus mutans at low Ca2+) was enhanced with increasing Ca2+ concentration. Lactobacillus casei was efficiently removed by adhesion on hydroxyapatite, even without Ca2+ addition, and the effect of this ion was only marginal. The results are interpreted in terms of Ca2+-mediated adhesion, and relative to the hydrophobic properties of each strain and the electrical properties of the bacterial and solid surfaces (electrophoretic mobility).

scholarly journals A Concise Review of Silver Diamine Fluoride on Oral Biofilm

2021 ◽  
Vol 11 (7) ◽  
pp. 3232
Author(s):  
Jingyang Zhang ◽  
Sofiya-Roksolana Got ◽  
Iris Xiaoxue Yin ◽  
Edward Chin-Man Lo ◽  
Chun-Hung Chu
Keyword(s):  
Clinical Study ◽  
Streptococcus Mutans ◽  
Bacterial Adhesion ◽  
Clinical Studies ◽  
Antibacterial Properties ◽  
Tooth Surface ◽  
Original Research ◽  
Laboratory Studies ◽  
Oral Biofilm ◽  
Silver Diamine Fluoride

Studies have shown that silver diamine fluoride (SDF) is an effective agent to arrest and prevent dental caries due to its mineralizing and antibacterial properties. While plenty of studies have investigated the mineralizing properties, there are few papers that have examined its antibacterial effect on oral biofilm. The objective of this study was to identify the effect of silver diamine fluoride on oral biofilm. Method: The keywords used were (silver diamine fluoride OR silver diammine fluoride OR SDF OR silver fluoride OR AgF AND biofilm OR plaque). Two reviewers screened the titles and abstracts and then retrieved the full text of the potentially eligible publications. Publications of original research investigating the effect of SDF on oral biofilm were selected for this review. Results: This review included 15 laboratory studies and six clinical studies among the 540 papers identified. The laboratory studies found that SDF could prevent bacterial adhesion to the tooth surface. SDF also inhibited the growth of cariogenic bacteria, including Streptococcus mutans, Lactobacillus acidophilus, Streptococcus sobrinus, Lactobacillus rhamnosus, Actinomyces naeslundii, and Enterococcus faecalis, thus contributing to its success in caries arrest. One clinical study reported a decrease in Streptococcus mutans and Lactobacillus sp. in arrested caries after SDF treatment, and another clinical study found that SDF inhibited the growth of periodontitis microbiota, including Porphyromonas gingivalis, Tannerella forsythia, and Prevotella intermedia/nigrescens. However, three clinical studies reported no significant change in the microbial diversity of the plaque on the tooth after SDF treatment. Moreover, one laboratory study and one clinical research study reported that SDF inhibited the growth of Candida albicans. Conclusion: Not many research studies have investigated the effects of SDF on oral biofilm, although SDF has been used as a caries-arresting agent with antibacterial properties. However, a few publications have reported that SDF prevented bacterial adhesion to the teeth, inhibited the growth of cariogenic and periodontal bacteria, and possessed antifungal properties.

scholarly journals Casein phosphopeptide acts synergistically with fluoride to inhibit initial adhesion of Streptococcus mutans to the saliva-coated hydroxyapatite disc

2020 ◽  
Author(s):  
Xiaodie Wang ◽  
Limin Liu ◽  
Xiaoyan Zhou ◽  
Yongbiao Huo ◽  
Jinlong Gao ◽  
...  
Keyword(s):  
Contact Angle ◽  
Zeta Potential ◽  
Streptococcus Mutans ◽  
Water Contact Angle ◽  
Bacterial Adhesion ◽  
Laser Scanning Microscopy ◽  
Water Contact ◽  
Initial Adhesion ◽  
Salivary Pellicle ◽  
Casein Phosphopeptide

Abstract Background Recent preventive strategies for dental caries focus on targeting the mechanisms underlying biofilm formation, including inhibiting bacterial adhesion. A promising approach to prevent bacterial adhesion is to modify the composition of acquired salivary pellicle. This in vitro study investigated the effect and possible underlying mechanism of pellicle modification by casein phosphopeptide (CPP) on Streptococcus mutans (S. mutans) initial adhesion, and the impact of fluoride on the efficacy of CPP.Methods The salivary pellicle-coated hydroxyapatite (s-HA) disc was modified by 2.5% CPP or 2.5% CPP supplemented with 900 ppm fluoride solutions. After cultivation of S. mutans, the adherent bacteria were visualized by scanning electron microscopy (SEM) and quantitatively evaluated using the plate count method. Confocal laser scanning microscopy (CLSM) was used to evaluate the proportions of total and dead S. mutans. The concentrations of total, free, and bound calcium and fluoride in CPP and fluoride-doped CPP solutions were determined. The water contact angle and zeta potential of s-HA with and without modification were measured. The data were statistically analyzed using one-way ANOVA followed by a Turkey post hoc multiple comparison test.Results Initial adhesion of S. mutans to s-HA was inhibited in response to pellicle modification by CPP and fluoride-doped CPP, and the latter was more efficient. CLSM analysis showed that the proportion of dead S. mutans did not differ between the groups. Water contact angle and zeta potential decreased after pellicle modification, and both were lowest in the fluoride-doped CPP group.Conclusions Pellicle modification by CPP inhibited S. mutans initial adhesion to s-HA, possibly by reducing hydrophobicity and negative charge on the s-HA surface, and incorporating fluoride into CPP enhanced its anti-adhesion effect.

Casein phosphopeptide combined with fluoride enhances the inhibitory effect on initial adhesion of Streptococcus mutans to the saliva-coated hydroxyapatite disc

2020 ◽  
Author(s):  
Xiaodie Wang ◽  
Limin Liu ◽  
Xiaoyan Zhou ◽  
Yongbiao Huo ◽  
Jinlong Gao ◽  
...  
Keyword(s):  
Contact Angle ◽  
Zeta Potential ◽  
Streptococcus Mutans ◽  
Water Contact Angle ◽  
Bacterial Adhesion ◽  
Laser Scanning Microscopy ◽  
Water Contact ◽  
Initial Adhesion ◽  
Salivary Pellicle ◽  
Casein Phosphopeptide

Abstract Background: Recent preventive strategies for dental caries focus on targeting the mechanisms underlying biofilm formation, including inhibiting bacterial adhesion. A promising approach to prevent bacterial adhesion is to modify the composition of acquired salivary pellicle. This in vitro study investigated the effect and possible underlying mechanism of pellicle modification by casein phosphopeptide (CPP) on Streptococcus mutans (S. mutans) initial adhesion, and the impact of fluoride on the efficacy of CPP. Methods: The salivary pellicle-coated hydroxyapatite (s-HA) discs were treated with phosphate buffered saline (blank control), heat-inactivated 2.5% CPP (negative control), 2.5% CPP (CPP) or 2.5% CPP supplemented with 900 ppm fluoride (CPP + F). After cultivation of S. mutans, the adherent bacteria were visualized by scanning electron microscopy (SEM) and quantitatively evaluated using the plate count method. Confocal laser scanning microscopy (CLSM) was used to evaluate the proportions of total and dead S. mutans. The concentrations of total, free, and bound calcium and fluoride in CPP and fluoride-doped CPP solutions were determined. The water contact angle and zeta potential of s-HA with and without modification were measured. The data were statistically analyzed using one-way ANOVA followed by a Turkey post hoc multiple comparison test.Results: Initial adhesion of S. mutans to s-HA was inhibited in response to pellicle modification by CPP and fluoride-doped CPP, and the latter was more efficient. CLSM analysis showed that the proportion of dead S. mutans did not differ between the groups. Water contact angle and zeta potential decreased after pellicle modification, and both were lowest in the CPP + F group. Conclusions: Pellicle modification by CPP inhibited S. mutans initial adhesion to s-HA, possibly by reducing hydrophobicity and negative charge on the s-HA surface, and incorporating fluoride into CPP enhanced its anti-adhesion effect.

scholarly journals Suppressive Effects of Octyl Gallate on Streptococcus mutans Biofilm Formation, Acidogenicity, and Gene Expression

Molecules ◽  
2019 ◽  
Vol 24 (17) ◽  
pp. 3170 ◽  
Author(s):  
Vika Gabe ◽  
Tomas Kacergius ◽  
Saleh Abu-Lafi ◽  
Mouhammad Zeidan ◽  
Basheer Abu-Farich ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dental Caries ◽  
Streptococcus Mutans ◽  
Biofilm Formation ◽  
Colorimetric Method ◽  
Solid Surfaces ◽  
Dependent Manner ◽  
Oral Diseases ◽  
Expression Change ◽  
Biofilm Development

The accumulation of biofilm by Streptococcus mutans bacteria on hard tooth tissues leads to dental caries, which remains one of the most prevalent oral diseases. Hence, the development of new antibiofilm agents is of critical importance. The current study reports the results from testing the effectiveness of octyl gallate (C8-OG) against: (1) S. mutans biofilm formation on solid surfaces (polystyrene, glass), (2) acidogenicity, (3) and the expression of biofilm-related genes. The amount of biofilm formed by S. mutans bacteria was evaluated using the colorimetric method and optical profilometry. The pH of the biofilm growth medium was measured with microelectrode. A quantitative reverse transcription-polymerase chain reaction (RT-qPCR) was used to assess the expression of genes encoding glucan binding protein B (gbpB), glucosyltransferases B, -C, -D (gtfB, -C, -D), and the F-ATPase β subunit of the F1 protein (atpD). The results show that C8-OG significantly diminished biofilm formation by exposed S. mutans on solid surfaces and suppressed acidogenicity in a dose-dependent manner, compared to unexposed bacteria (p < 0.05). The C8-OG concentration of 100.24 µM inhibited S. mutans biofilm development on solid surfaces by 100% and prevented a decrease in pH levels by 99%. In addition, the RT-qPCR data demonstrate that the biofilm-producing bacteria treated with C8-OG underwent a significant reduction in gene expression in the case of the four genes under study (gbpB, gtfC, gtfD, and atpD), and there was a slight decrease in expression of the gtfB gene. However, C8-OG treatments did not produce significant expression change compared to the control for the planktonic cells, although there was a significant increase for the atpD gene. Therefore, C8-OG might be a potent antibiofilm and/or anticaries agent for oral formulations that aim to reduce the prevalence of dental caries.

Effects of calcium oxide on the surface properties of industrial sludge by analyzing rheological and electrical properties

2020 ◽  
Vol 8 (3) ◽  
pp. 103764 ◽  
Author(s):  
A. Mortadi ◽  
A. Elmelouky ◽  
El G. Chahid ◽  
R. Essajai ◽  
H. Naserllah ◽  
...  
Keyword(s):  
Electrical Properties ◽  
Surface Properties ◽  
Calcium Oxide ◽  
Industrial Sludge

scholarly journals Chain Length of Amphipathic-Type Thioesters Dramatically Affects Reactivity in Aqueous Amidation Reactions with Cysteine Esters

SynOpen ◽  
2017 ◽  
Vol 01 (01) ◽  
pp. 0059-0062
Author(s):  
Ikumi Otomo ◽  
Kanna Watanabe ◽  
Chiaki Kuroda ◽  
Kenichi Kobayashi
Keyword(s):  
Amino Acid ◽  
Aqueous Medium ◽  
Chain Length ◽  
Ethyl Esters ◽  
Side Chain ◽  
Amino Acid Side Chain ◽  
Hydrophobic Properties ◽  
M 10 ◽  
Hexyl Ester ◽  
Close Proximity

The reaction of five amphipathic-type thioesters, CH3(CH2) m COS(CH2) n COONa (m + n = 12), with cysteine hexyl, butyl, and ethyl esters were studied in aqueous medium. Compounds with the thioester group in close proximity to the carboxylate moiety (m = 10, n = 2) afforded amides in almost quantitative yield, whereas no reaction proceeded by using compounds with the thioester group distant from the carboxylate. In contrast, no clear difference in yield was observed among the five amphipathic-type thioesters upon reaction with valine hexyl ester. The results indicate that the reaction is affected by both the position of the thioester group and the hydrophilic/hydrophobic properties of the amino acid side chain.

Surface properties and bacterial adhesion of bulk-fill composite resins

2020 ◽  
Vol 95 ◽  
pp. 103317
Author(s):  
Dilber Bilgili ◽  
Ayşe Dündar ◽  
Çağatay Barutçugil ◽  
Deniz Tayfun ◽  
Özlem Koyuncu Özyurt
Keyword(s):  
Surface Properties ◽  
Bacterial Adhesion ◽  
Composite Resins
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