Vitamin C Induces the Reduction of Oxidative Stress and Paradoxically Stimulates the Apoptotic Gene Expression in Extravillous Trophoblasts Derived From First-Trimester Tissue

The relationship between oxidative stress and miRNA changes in placenta as a potential mechanism involved in preeclampsia (PE) is not fully elucidated. We investigated the impact of oxidative stress on miRNAs and mRNA expression profiles of genes associated with PE in villous 3A first trimester trophoblast cells exposed to H2O2at 12 different concentrations (0-1 mM) for 0.5, 4, 24, and 48 h. Cytotoxicity, determined using the SRB assay, was used to calculate the IC50of H2O2. RNA was extracted after 4 h exposure to H2O2for miRNA and gene expression profiling. H2O2exerted a concentration- and time-dependent cytotoxicity on 3A trophoblast cells. Short-term exposure of 3A cells to low concentration of H2O2(5% of IC50) significantly altered miRNA profile as evidenced by significant changes in 195 out of 595 evaluable miRNAs. Tool for annotations of microRNAs (TAM) analysis indicated that these altered miRNAs fall into 43 clusters and 34 families, with 41 functions identified. Exposure to H2O2altered mRNA expression of 22 out of 84 key genes involved in dysregulation of placental development. In conclusion, short-term exposure of villous first trimester trophoblasts to low concentrations of H2O2significantly alters miRNA profile and expression of genes implicated in placental development.

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Zearalenone nephrotoxicity: DNA fragmentation, apoptotic gene expression and oxidative stress protected by Lactobacillus plantarum MON03

Toxicon ◽

10.1016/j.toxicon.2019.12.004 ◽

2020 ◽

Vol 175 ◽

pp. 28-35 ◽

Cited By ~ 2

Author(s):

Jalila Ben Salah-Abbès ◽

Hela Belgacem ◽

Khawla Ezzdini ◽

Mosaad A. Abdel-Wahhab ◽

Samir Abbès

Keyword(s):

Oxidative Stress ◽

Gene Expression ◽

Dna Fragmentation ◽

Lactobacillus Plantarum ◽

Apoptotic Gene ◽

And Oxidative Stress

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Evaluating the Effect of Vitamin C on Myocardial Angiogenesis Under Oxidative Stress Induced by Exhaustive Exercise in Rat

Pharmaceutical Sciences ◽

10.15171/ps.2018.40 ◽

2018 ◽

Vol 24 (4) ◽

pp. 273-279

Author(s):

Parivash Piraki ◽

Ahmad Hemmatfar ◽

Mohammad Ali Samavati Sharif ◽

Naser Behpour

Keyword(s):

Oxidative Stress ◽

Gene Expression ◽

Vitamin C ◽

Early Stage ◽

Daily Intake ◽

Heart Tissue ◽

Beneficial Effects ◽

Myocardial Oxidative Stress ◽

Serum Creatine Phosphokinase ◽

Antioxidant Parameters

Background: The main purpose of the present study was to assess the effects of exhaustive swimming with the consumption of a vitamin C supplement on indices of myocardial oxidative stress and gene expression related to angiogenesis. Methods: Wistar rats were randomly divided into six groups of normal (C), 100 and 200 mg/kg of vitamin C, (VC100 and VC200), exercise with 100 and 200 mg/kg of vitamin C (Ex+VC100 and Ex+VC200) and exercise without treatment (Ex). Finally, the serum activity of serum creatine phosphokinase (CK) and lactate dehydrogenase (LDH) and heart tissue oxidant/antioxidant parameters, besides gene expression of Vascular endothelial growth factor-B (VEGF-B), angiopoietin 1 (ANGPT-1) and matrix metalloproteinases 2 (MMP-2) was measured. Results: Significant increase in LDH level was seen in group Ex which was remarkably attenuated in group Ex+VC200 (p<0.001). The tissue oxidative stress was observed in group Ex where daily intake of vitamin C could remarkably regulate this property (p<0.01). Vitamin C could ameliorate significant upper gene expression of VEGF-B and MMP-2 remarkably (p<0.05). Conclusion: Oxidative condition in myocardial besides over expression of MMP-2, could be concluded as a detrimental condition resulting from exhaustive swimming that continued by the proteolytic release of CK and LDH from the muscle. Upper gene expression of VEGF-B and MMP-2 besides no changes of ANGPT-1 can be concluded as an early stage of angiogenesis. All these events were somehow attenuated by vitamin C which confirmed its beneficial effects as an antioxidant and the role of oxidation properties in the regulation of angiogenesis.

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Nephroprotective effect of silymarin against diclofenac induced renal damage and oxidative stress in male rats

Journal of Herbmed Pharmacology ◽

10.15171/jhp.2019.23 ◽

2019 ◽

Vol 8 (2) ◽

pp. 146-152 ◽

Cited By ~ 7

Author(s):

Ali Nouri ◽

Esfandiar Heidarian

Keyword(s):

Oxidative Stress ◽

Gene Expression ◽

Vitamin C ◽

Group Treatment ◽

Renal Damage ◽

Male Rats ◽

Tissue Homogenate ◽

Control Group ◽

Tnf Α ◽

And Oxidative Stress

Introduction: Diclofenac (DIC), a phenylacetic acid compound which belongs to nonsteroidal anti-inflammatory drugs (NSAIDs), is generally used for the treatment of various diseases such as rheumatoid arthritis, ankylosing spondylitis, acute muscle pain conditions and osteoarthritis. Overdose of DIC can lead to renal injuries in both experimental animal and human. Our research was done to assess the protective role of silymarin on renal damage induced by DIC in rats. Methods: Thirty-two Wistar rats were assigned to four groups (n=8/group). Group 1 was control group; animals in group 2 were administrated DIC; Groups 3 and 4 administrated DIC plus silymarin with doses of 100 mg/kg and 200 mg/kg, orally (p.o), respectively. Various biochemical, molecular, and histological parameters were evaluated in serum and tissue homogenate. Results: In the second group, the levels of kidney catalase (CAT), vitamin C and superoxide dismutase (SOD) remarkably reduced (P < 0.05) relative to the control group. Also, urea, creatinine (Cr), malondialdehyde (MDA), serum tumor necrosis factor-α (TNF-α) and gene expression of TNF-α in this group were noticeably elevated (P < 0.05) relative to the control group. Treatment with silymarin caused a remarkable elevation (P < 0.05) in vitamin C, SOD, CAT and a remarkable reduction (P < 0.05) in the content of MDA, urea, Cr, TNF-α gene expression and serum TNF-α in comparison with second group. Histological injuries were also ameliorated by silymarin administration. Conclusion: The results confirm that silymarin has an ameliorative role against renal damage and oxidative stress induced by DIC in male rats.

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Effect of glucose mediated oxidative stress on apoptotic gene expression in gingival mesenchymal stem cells

BMC Oral Health ◽

10.1186/s12903-021-02007-y ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Rabiya Junaid ◽

Mohsin Wahid ◽

Farzeen S. Waseem ◽

Rakhshinda Habib ◽

Arshad Hasan

Keyword(s):

Oxidative Stress ◽

Gene Expression ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Growth Kinetics ◽

High Glucose ◽

Cellular Proliferation ◽

Common Disease ◽

Apoptotic Pathway ◽

Apoptotic Gene

Abstract Background Diabetes is a common disease that causes gingival and periodontal problems. Stem cells isolated from dental sources are an emerging area of research with a potential to facilitate regenerative medicine. The stem cells retain the property of self-renewal and the ones isolated from dental sources are mainly multipotent mesenchymal stem cells that have the ability to self-renew as well as differentiation towards multiple lineages. Objectives We aimed to isolate and characterize gingival mesenchymal stem cells by pluripotency markers and investigated the effect of oxidative stress on growth kinetics and apoptotic gene expression of gingival cells exposed to glucose mediated oxidative stress. Methods In this study, we isolated gingival mesenchymal stem cells from gingiva. This was followed by morphologic analysis using inverted phase contrast microscopy and molecular profiling of these cells for the mRNA expression of specific genes. The isolated cells were cultured till passage 3 and then exposed to oxidative stress (high glucose concentration). We measured the apoptotic gene expression and compared their growth kinetics. Results The results showed that oxidative stress produced by glucose reduced growth kinetics and increased apoptotic gene expression in gingival mesenchymal stem cells. According to the genetic results, glucose activated TNF family to initiate apoptosis. Conclusion In conclusion, the present study demonstrated that high glucose obliterated cellular proliferation testified by evaluating growth kinetics and induced apoptotic gene expression in gingival mesenchymal stem cells. This initiated extrinsic apoptotic pathway mediated by TNF family. Therefore, in diabetes oral health condition is compromised and periodontal disease is common.

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The Protective Impacts of Spriulina Platensis Against Cisplatin-Induced Renal Injury Through The Regulation of Oxidative Stress, Pro-Inflammatory Cytokines and Bax/Bcl2 Expression Cascade

10.21203/rs.3.rs-109660/v1 ◽

2020 ◽

Author(s):

Tarek Abouzed ◽

Mohamed Soliman ◽

Shymaa Khatab ◽

Ahmed Elgazzar ◽

Wael Gouda ◽

...

Keyword(s):

Oxidative Stress ◽

Gene Expression ◽

The Body ◽

Albino Rats ◽

Oxidative Stress Biomarkers ◽

Apoptotic Gene ◽

Group 4 ◽

Renal Histopathology ◽

Renal Markers ◽

Group 2

Abstract One of the main antineoplastic chemotherapy medications is cisplatin; nephropathy is a major side effect of cisplatin. The current study investigates the molecular protective effect of Spirulina Platensis (SP) on cisplatin-induced nephrotoxicity. Forty eight healthy male albino rats were allocated into 4 groups. Group 1 received saline intraperitoneally (IP) twice per week (normal rats). Group 2, received SP (100 mg/kg bw orally). Group 3 injected cisplatin (1.5mg/kg IP) twice per week. Group 4 received SP and at 4th day received cisplatin (1.5mg/kg IP) for 21 days. After 3 weeks of experimentation, blood and renal tissues were taken for serum analysis, gene expression using qRT-PCR and renal histopathology. SP significantly ameliorated the alterations in the body weight, relative kidney weight, and the disturbance in examined renal markers. Furthermore oxidative stress biomarkers (MDA, NO, SOD, and GSH) induced by cisplatin were recovered and restored by SP. Cisplatin induced upregulation in the gene expression of TNF-α, iNOS, TGF1-β, IL-1β and IL-6 that were ameliorated by pre-administration of SP. Finally, cisplatin upregulated pro-apoptotic gene; Bax and downregulated anti-apoptotic gene; Bcl2. Of interest, SP mitigated this alteration in apoptosis and anti-apoptosis associated genes. Renal histopathology revealed the protective impacts of SP against cisplatin induced severe glomerular congestion, hemorrhage, inflammatory cell infiltration, degeneration and sever necrosis in renal glomeruli and tubules. In conclusion, SP has protective impact against cisplatin induced renal damage through the modulation of oxidative stress, anti-inflammatory, anti-necrotic and anti-apoptotic associated genes.

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