scholarly journals Antibacterial Activity of the Essential Oil of Lippia oreganoides against Multiresistant Bacterial Strains of Nosocomial Origin

2007 ◽  
Vol 2 (1) ◽  
pp. 1934578X0700200 ◽  
Author(s):  
Judith Velasco ◽  
Janne Rojas ◽  
Poema Salazar ◽  
Mariseg Rodríguez ◽  
Tulia Díaz ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Low Dose ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Pharmaceutical Preparations ◽  
Bacterial Strains ◽  
Multiresistant Bacteria ◽  
Agar Method ◽  
First Time

Antibacterial activity of the essential oil of Lippia oreganoides was evaluated against thirty-nine multiresistant bacterial strains of nosocomial origin and five reference bacterial strains using the disk diffusion agar method. The results obtained have revealed strong antibacterial activity against methicillin resistant Staphylococcus aureus, extended spectrum β-lactamase producing Klebsiella pneumoniae, and multiresistant Acinetobacter baumannii with MIC values ranging from 20 to 40 μg/mL. The low dose antibacterial activity observed in this investigation suggested that the essential oil of L. oreganoides could be used in pharmaceutical preparations for the treatment of infections caused by multiresistant bacteria. According to the literature consulted, this is the first time that antibacterial activity of this species against multiresistant bacterial strains of nosocomial origin has been reported.

scholarly journals Synergy between Essential Oils of Calamintha Species (Lamiaceae) and Antibiotics

2018 ◽  
Vol 13 (3) ◽  
pp. 1934578X1801300 ◽  
Author(s):  
Marina Milenković ◽  
Jelena Stošović ◽  
Violeta Slavkovska
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Broth Microdilution ◽  
Bacterial Strains ◽  
Microdilution Method ◽  
Broth Microdilution Method ◽  
The Subject

The subject of the study was the investigation of the chemical composition and antimicrobial activity of the essential oils (EOs) isolated from Calamintha sylvatica, C. vardarensis, C. nepeta and C. glandulosa, as well as their antibacterial activity in combination with antibiotics. The quantitative and qualitative analysis of EOs was performed using the GC/FID and GC/MS methods. The antimicrobial activity of EOs against six standard bacterial strains and one strain of yeast was tested using the broth microdilution method, while the antimicrobial activity of a combination of essential oils and gentamicin/ciprofloxacin was tested by the checkerboard method. The dominant components (> 10%) of the essential oils were: cis-piperitone epoxide and menthone ( C. sylvatica), pulegone and menthone ( C. vardarensis), pulegone and piperitenone ( C. nepeta), pulegone, piperitenone, menthone and piperitone ( C. glandulosa). EOs did not exhibit significant antimicrobial activity except the essential oil of C. vardarensis which was selectively active against Staphylococcus aureus (MIC - 21.25 μg/mL). The overall effect of essential oil-antibiotic combinations varied from synergistic (FICI ≤ 0.5) to antagonistic (FICI ≥ 2) depending on the bacterial strain tested.

scholarly journals Chemical Composition and Antibacterial Activity of the Essential Oil of Monticalia andicola (Asteraceae) Collected in Venezuela

2009 ◽  
Vol 4 (11) ◽  
pp. 1934578X0900401 ◽  
Author(s):  
Shirley Baldovino ◽  
Janne Rojas ◽  
Luis B. Rojas ◽  
María Lucena ◽  
Alexis Buitrago ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Mass Spectra ◽  
Inhibitory Concentration ◽  
Disc Diffusion ◽  
Gram Negative ◽  
Agar Method

The essential oil from the leaves of Monticalia andicola Turcz., collected in November 2008, was analyzed by GC/MS. A yield of 0.15% oil was obtained by hydrodistillation. Thirty-six components were identified by comparison of their mass spectra with those in the Wiley GC-MS Library data base. The major components were α-pinene (19.6%), β-pinene (10.5%), α-longipinene (6.5%), δ-3-carene (6.2%), cyperene (5.4%) and β-phellandrene (5.2%). The antibacterial activity of the essential oil was evaluated against Gram- positive (Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212) and Gram-negative (Escherichia coli ATCC 25992, Klebsiella pneumoniae ATCC 23357, Pseudomonas aeruginosa ATCC 27853) bacteria, using the disc diffusion agar method. The results showed a broad spectrum of activity with minimal inhibitory concentration (MIC) values ranging from 10 to150 μg/mL.

scholarly journals Chemical Profiling, Antioxidant and Antibacterial Activities of Essential Oil From Englerastrum gracillimum Th. C. E. Fries Growing in Niger

2021 ◽  
Vol 16 (3) ◽  
pp. 1934578X2110024
Author(s):  
Bala Namata Abba ◽  
Amadou Tidjani Ilagouma ◽  
Issoufou Amadou ◽  
Abderrahmane Romane
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Radical Scavenging ◽  
Antibacterial Activities ◽  
Antioxidant Power ◽  
Methicillin Resistant ◽  
Agar Disc

In the present study, the chemical composition, antioxidant and antibacterial activities of the essential oil obtained by hydrodistillation of the aerial parts of Englerastrum gracillimum Th. C. E. Fries growing in Niger were investigated. Gas chromatography-mass spectrometry (GC–MS) analysis of the essential oil resulted in the identification of 42 compounds representing 97.9% of the total oil constituents. The major compounds of the essential oil were: α-humulene (30.5%), followed by cubenol (19.8%), γ-muurolene (14.0%), ( E)-β-caryophyllene (5.8%), β-gurjunene (5.2%), and curzerene (4.9%). The antioxidant activity of the essential oil was determined by using the free radical-scavenging activity (2,2 diphenyl-1-picrylhydrazyl: DPPH˙) and ferric reducing antioxidant power assays. The essential oil showed good antioxidant potential with both methods. The antibacterial activity was evaluated against multi-resistant Acinetobacter baumannii P1483, extended-spectrum β-lactamase (ESBL) -Escherichia coli Bu8566, Salmonella spp. H1548, Proteus mirabilis Bu190 , Enterobacter cloacae Bu147, Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922), Klebsiella pneumoniae (ATCC 700603), methicillin-resistant Staphylococcus aureus P1123, Enterococcus faecium H3434, and Staphylococcus aureus (ATCC 25923) using the agar disc diffusion and microdilution methods. The essential oil exhibited good antibacterial activity. The highest antibacterial effect was observed against Staphylococcus aureus ATCC 25923 and methicillin-resistant Staphylococcus aureus P1123 with minimal inhibitory concentration (MIC) values of 0.03 mg/mL and 0.06 mg/mL, respectively. These data are of scientific importance for the valorization of aromatic and medicinal plants of Niger, especially E. gracillimum. This study reports for the first time the antioxidant and antibacterial activities of this essential oil.

scholarly journals Chemical Composition of Argentinean Propolis Collected in Extreme Regions and its Relation with Antimicrobial and Antioxidant Activities

2011 ◽  
Vol 6 (6) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Nancy Vera ◽  
Eliana Solorzano ◽  
Roxana Ordoñez ◽  
Luis Maldonado ◽  
Enrique Bedascarrasbure ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Bioactive Compounds ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Antioxidant Activities ◽  
Isolation And Identification ◽  
Microdilution Method ◽  
First Time ◽  
Β Carotene

This paper reveals, for the first time, the functional properties of propolis from an extreme region of Argentine ( El Rincón, Province of Catamarca, Argentina), as well as the isolation and identification of bioactive compounds. The antioxidant activity was determined by the ABTS method and β-carotene bleaching. The antibacterial activity was determined on methicillin resistant Staphylococcus aureus (MRSA) by the microdilution method and bioautographic assays. Twelve compounds were isolated and identified by NMR spectroscopy. The main bioactive compounds were 2′,4′-dihydroxy-3′-methoxychalcone (3), 2′,4′-dihydroxychalcone (9), 2′,4′,4-trihydroxy-6′- methoxychalcone (8), 5-hydroxy-4′,7-dimethoxyflavone (4), 4′,5-dihydroxy-3,7,8-trimethoxyflavone (10) and 7-hydroxy- 5,8-dimethoxyflavone (11). All compounds were active against clinical isolates (MIC50 10 μg/ mL) and displayed antioxidant activity (SC50 values of 20 μg/mL). The MIC and SC50 values of the isolated compounds were lower than those obtained with crude propolis extracts, chloroform sub-extracts and isolated fractions.

scholarly journals GC-FID Analysis and Antibacterial Activity of the Calyptranthes concinna Essential Oil against MDR Bacterial Strains

Separations ◽  
2020 ◽  
Vol 7 (1) ◽  
pp. 10
Author(s):  
Maria do Socorro Costa ◽  
Nara Juliana Santos Araújo ◽  
Thiago Sampaio de Freitas ◽  
Francisco Assis Bezerra da Cunha ◽  
Wanderlei do Amaral ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Inhibitory Concentration ◽  
Chemical Constituents ◽  
Antibiotic Activity ◽  
Bacterial Strains ◽  
Microdilution Method ◽  
Multiresistant Bacteria ◽  
Broth Microdilution Method ◽  
Mic Value

Presently, results from a study carried out in this area using the essential oil from the Calyptranthes concinna species, a representative from the Myrtaceae family, are reported. The essential oil was obtained by hydrodistillation and gas chromatography coupled to mass spectrometry was used to identify its chemical constituents. Antibacterial activity was determined using the broth microdilution method, thus obtaining the Minimal Inhibitory Concentration (MIC) value, from which the subinhibitory concentration (MIC/8) was derived. The C. concinna essential oil presented antibacterial activity against both standard and multiresistant bacteria. In addition, the oil demonstrated an antibiotic activity potentiation against Staphylococcus aureus and Escherichia coli when in combination with the antibiotic gentamicin, reducing the MIC from 141.38 μg/mL and 208.63 μg/mL to 64 μg/mL and 128 μg/mL, respectively. Conclusions: Findings from the present study suggest this oil is promising in terms of its antimicrobial activity.

scholarly journals Cysteines and Disulfide-Bridged Macrocyclic Mimics of Teixobactin Analogues and Their Antibacterial Activity Evaluation against Methicillin-Resistant Staphylococcus Aureus (MRSA)

Pharmaceutics ◽  
2018 ◽  
Vol 10 (4) ◽  
pp. 183 ◽  
Author(s):  
Ruba Malkawi ◽  
Abhishek Iyer ◽  
Anish Parmar ◽  
Daniel Lloyd ◽  
Eunice Leng Goh ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Building Blocks ◽  
Disulfide Bridge ◽  
Methicillin Resistant ◽  
Macrocyclic Ring ◽  
Gram Positive Bacteria ◽  
Ester Linkage ◽  
First Time

Teixobactin is a highly potent cyclic depsipeptide which kills a broad range of multi-drug resistant, Gram-positive bacteria, such as Methicillin-resistant Staphylococcus aureus (MRSA) without detectable resistance. In this work, we describe the design and rapid synthesis of novel teixobactin analogues containing two cysteine moieties, and the corresponding disulfide-bridged cyclic analogues. These analogues differ from previously reported analogues, such as an Arg10-teixobactin, in terms of their macrocyclic ring size, and feature a disulfide bridge instead of an ester linkage. The new teixobactin analogues were screened against Methicillin-resistant Staphylococcus aureus and Methicillin-sensitive Staphylococcus aureus. Interestingly, one teixobactin analogue containing all l-amino acid building blocks showed antibacterial activity against MRSA for the first time. Our data indicates that macrocyclisation of teixobactin analogues with disulfide bridging is important for improved antibacterial activity. In our work, we have demonstrated the unprecedented use of a disulfide bridge in constructing the macrocyclic ring of teixobactin analogues.

Antioxidant and Antibacterial Activities of Artemisia herba-alba Asso Essential Oil from Middle Atlas, Morocco

2018 ◽  
Vol 16 (S1) ◽  
pp. S48-S54
Author(s):  
Y. Ez zoubi ◽  
S. Lairini ◽  
A. Farah ◽  
K. Taghzouti ◽  
A. El Ouali Lalami
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Foodborne Pathogens ◽  
Food Systems ◽  
Antibacterial Activities ◽  
Culture Collection ◽  
Bornyl Acetate ◽  
Bacterial Strains ◽  
Disk Diffusion Assay ◽  
Different Strains

The purpose of this study was to determine the chemical composition and to evaluate the antioxidant and antibacterial effects of the Moroccan Artemisia herba-alba Asso essential oil against foodborne pathogens. The essential oil of Artemisia herba-alba was analyzed by gas chromatography coupled with mass spectroscopy. The antibacterial activity was assessed against three bacterial strains isolated from foodstuff and three bacterial strains referenced by the ATCC (American Type Culture Collection) using the disk diffusion assay and the macrodilution method. The antioxidant activity was evaluated using the DPPH (2, 2-diphenyl-1- picrylhydrazyl) method. The fourteen compounds of the Artemisia herba-alba essential oil were identified; the main components were identified as β-thujone, chrysanthenone, α-terpineol, α-thujone, α-pinene, and bornyl acetate. The results of the antibacterial activity obtained showed a sensitivity of the different strains to Artemisia herba-alba essential oil with an inhibition diameter of 8.50 to 17.00 mm. Concerning the MICs (minimum inhibitory concentrations), the essential oil exhibited much higher antibacterial activity with MIC values of 2.5 μl/ml against Bacillus subtilis ATCC and Lactobacillus sp. The essential oil was found to be active by inhibiting free radicals with an IC50 (concentration of an inhibitor where the response is reduced by half) value of 2.9 μg/ml. These results indicate the possible use of the essential oil on food systems as an effective inhibitor of foodborne pathogens, as a natural antioxidant, and for potential pharmaceutical applications. However, further research is needed in order to determine the toxicity, antibacterial, and antioxidant effects in edible products.

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