scholarly journals New 2-(2-Phenylethyl)chromone Derivatives from the Seeds of Cucumis melo L var. reticulatus

2010 ◽  
Vol 5 (3) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Sabrin R. M. Ibrahim
Keyword(s):  
Antimicrobial Activity ◽  
Cytotoxic Activity ◽  
Brine Shrimp ◽  
Cucumis Melo ◽  
Spectroscopic Data ◽  
Methanolic Extract ◽  
2D Nmr ◽  
Chromone Derivatives ◽  
Methanolic Extracts ◽  
Cucumis Melo L

Chemical investigation of the methanolic extract of the seeds of Cucumis melo L. var. reticulatus (Cucurbitaceae) afforded three new chromone derivatives; 5,7-dihydroxy-2-[2-(4-hydroxyphenyl)ethyl]chromone 3, 5,7-dihydroxy-2-[2-(3,4-dihydroxyphenyl)ethyl]chromone 4, and 7-glucosyloxy-5-hydroxy-2-[2-(4-hydroxyphenyl)ethyl]chromone 6, together with three known compounds; β-amyrin 1, β-sitosterol 2, and β-sitosterol-3- O-β-glucopyranoside 5. Their structures were established by UV, IR, 1D and 2D NMR, in addition to mass spectroscopic data and comparison with literature data. The n-hexane and methanolic extracts were evaluated for their antimicrobial activity, as well as cytotoxic activity using the brine shrimp bioassay.

PRELIMINARY PHYTOCHEMICAL AND ANTIMICROBIAL SCREENING OF UDHARA VAYUHARA CHURNA

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (06) ◽  
pp. 64-67
Author(s):  
Maruthi T. Ekbote ◽  
◽  
Rajashekar K. V ◽  
Shankarappa L ◽  
Bharathi D. R. ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Methanolic Extract ◽  
Acorus Calamus ◽  
Aqueous Extracts ◽  
Piper Longum ◽  
Polyherbal Formulation ◽  
Methanolic Extracts ◽  
Phytochemical Investigation

Ayurvedic medicines are being used increasingly to fight or prevent common diseases. Udhara vayuhara churna is a polyherbal formulation containing Piper longum (Piperceae) Zingiber officinalis (Zingiberaceae), Elettaria cardamom (Zingiberaceae), Plumbago zeylanica (Plumbaginaceae), Carum carvi (Umbelliferae), Acorus calamus (Araceae) and Embilica officinalis (Euphorbiaceae). Methanolic and aqueous extracts of Udhara vayuhara churna were screened for their antimicrobial activity against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Aspergillus niger and Candida albicans. The results of antimicrobial activity of aqueous and methanolic extracts of the Udhara vayuhara churna indicated that methanolic extract inhibited the growth of one or more test pathogens than aqueous extract. Udhara vayuhara churna formulation extracts showed a broad spectrum of antimicrobial activity. Phytochemical investigation revealed the presence of tannins, saponins, alkaloids, glycosides, flavonoids and essential oils. This may be due to the multifunctional effect of all the seven plant ingredients of Udhara Vayuhara churn.

Antimicrobial activity of Pycnarrhena cauliflora (Miers.) Diels. methanol extract

2021 ◽  
Vol 2 (2) ◽  
Author(s):  
Eti Nurwening Sholikhah ◽  
Maulina Diah ◽  
Mustofa ◽  
Masriani ◽  
Susi Iravati ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Methanolic Extract ◽  
Biological Activities ◽  
Positive Control ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Methanolic Extracts

Pycnarrhena cauliflora (Miers.) Diels., local name sengkubak, is one of indigenous plants from West Kalimantan that has been used as natural flavor. Pycnorrhena cauliflora is one of species of Menispermaceae family which is rich in bisbenzylisoquinoline alkaloids. This alkaloids are known to have various biological activities including antiprotozoal, antiplasmodial, antifungal and antibacterial activities. This study aimed to investigate antimicrobial activity of  the P. cauliflora (Miers.) Diels. methanolic extracts against gram-positive and gram-negative bacteria. The methanolic extract of P. cauliflora (Miers.) Diels., root, leaf and stem were prepared by maceration. The disk-diffusion method was then used to determine the antimicrobial activity of the extracts against Streptococcus pyogenes, S. mutants, Staphylococcus aureus, S. epidermidis, Salmonella typhi, Shigella flexneri, Pseudomonas aeruginosa and Escherichia coli after 18-24 h incubation at 37 oC. Amoxicillin was used as positive control for gram-positive bacteria and ciprofloxacin was used as gram-negative bacteria. The inhibition zones were then measured in mm. Analysis were conducted in duplicates. The results showed in general the methanolic extracts of P. cauliflora (Miers.) Diels. root (inhibition zone diameter= 10-23 mm) were more active than that leaf (0-15 mm) and stem (0-17 mm) extracts against gram-positive bacteria. The zone inhibition diameter of amoxicillin as positive control was 8-42 mm. In addition, the methanolic extracts of P. cauliflora (Miers.) Diels. root (12-17 mm) were also more active than that leaf (0-12 mm) and stem (0-12 mm) extracts against gram-negative bacteria. The zone inhibition diameter of ciprofloxacin as positive control was 33-36 mm. In conclusion, the methanolic extract of P. caulifloria (Miers.) Diels. root is the most extract active against both gram-positive and gram-negative bacteria. Further study will be focused to isolate active compounds in the methanolic extract of the root.

scholarly journals A New Cytotoxic Sesquiterpene and Three Anti-inflammatory Flavonoids from Egyptian Tanacetum Santolinoides

2007 ◽  
Vol 2 (11) ◽  
pp. 1934578X0700201 ◽  
Author(s):  
Sabrin R. M. Ibrahim ◽  
Jihan M. Badr ◽  
Khalid A. El Sayed ◽  
Diaa T. A. Youssef
Keyword(s):  
Cytotoxic Activity ◽  
Brine Shrimp ◽  
Methanolic Extract ◽  
Spectral Characteristics ◽  
Mass Spectral ◽  
Aerial Parts ◽  
Paw Oedema ◽  
Anti Inflammatory ◽  
Rat Paw ◽  
Rat Paw Oedema

A re-investigation of the methanolic extract of the aerial parts of Tanacetum santolinoides has resulted in the isolation of a new sesquiterpene, tanacetonic acid (1), along with three known methoxylated flavonoids. The structure of the isolated compounds was secured based on their IR, UV, 1D and 2D (1H-1H COSY, HSQC and HMBC) NMR spectroscopic and FAB mass spectral characteristics. The new sesquiterpene showed a significant cytotoxic activity when tested using the brine shrimp bioassay, while the three flavonoids demonstrated anti-inflammatory effects in the rat paw oedema test.

scholarly journals Steroids from the Leave of Persicaria perfoliata (L.) H. Gross

2019 ◽  
Vol 22 (2) ◽  
pp. 224-227
Author(s):  
Md Sagar Hossain ◽  
NM Mofiz Uddin Khan ◽  
Md Saiful Quddus ◽  
AM Sarwaruddin Chowdhury
Keyword(s):  
Antimicrobial Activity ◽  
Cytotoxic Activity ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Soluble Fraction ◽  
Pharmaceutical Journal ◽  
Antimicrobial Screening ◽  
Brine Shrimp Lethality ◽  
Persicaria Perfoliata ◽  
Test Organisms

β-sitosterol (1) and β-sitosterol-D-glucoside (2) were isolated from the dichloromethane soluble fraction of a methanol extract of the leave of Persicaria perfoliata. The crude fraction was subjected to antimicrobial screening and brine shrimp lethality bioassay, where moderate antimicrobial activity was observed against most of the test organisms was seen. The hexane soluble fraction also displayed significant cytotoxic activity with LC50 0.64 μg/ml in brine shrimp lethality bioassay. This is the first report of occurrence of these compounds from this plant. Bangladesh Pharmaceutical Journal 22(2): 224-227, 2019

scholarly journals New Chromone and Triglyceride from Cucumis melo Seeds

2014 ◽  
Vol 9 (2) ◽  
pp. 1934578X1400900
Author(s):  
Sabrin R. M. Ibrahim
Keyword(s):  
Antioxidant Activity ◽  
Cytotoxic Activity ◽  
Cucumis Melo ◽  
Spectral Measurements ◽  
Dpph Assay ◽  
Meoh Extract ◽  
Mass Spectral ◽  
Cucumis Melo L ◽  
First Time

Re-investigation of the MeOH extract of the seeds of Cucumis melo L. var. reticulatus (Cucurbitaceae) led to the isolation of a new chromone derivative (5,7-dihydroxy-2-[2-(3-methoxy-4-hydroxyphenyl)ethyl]chromone (5) and a triglyceride (1,3-di-(6 Z,9 Z)-docosa-6,9-dienoyl-2-(6 Z) hexacos-6-enoylglycerol (1), together with three known compounds; α-spinasterol (2), stigmasta-7,22,25-trien-3-ol (3), and D:B-friedoolean-5-ene-3-β-ol (4), are reported from this species for the first time. Their structures were determined by extensive 1D (1H, 13C, and DEPT) and 2D (1H-1H COSY, HMQC, and HMBC) NMR and mass spectral measurements. Compound 5 displayed significant cytotoxic activity against L5178Y cells, with an ED50 of 5 μM. The MeOH extract and 5 showed antioxidant activity using the DPPH assay.

scholarly journals Antimicrobial Activity of Opuntia cochenillifera (L.) Mill Fruit and Cladode Extracts

2016 ◽  
Vol 3 ◽  
pp. 84-89
Author(s):  
Pooja Suryawanshi ◽  
G.M. Vidyasagar
Keyword(s):  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Methanolic Extract ◽  
Maximum Activity ◽  
Methanolic Extracts ◽  
Agar Well Diffusion Assay ◽  
Diffusion Assay

In the present study the antimicrobial activity of chloroform and methanolic extracts of Opuntiacochenillifera for both cladode and fruits was investigated. Methanolic extract was found to be an effective against the microbes namely, E. coli,Bacillus subtilis,Staphylococcus aureus, Pseudomonas aeruginosaCandidaalbicanC.glabrataC.haemuloniiC.Tropicalis. Maximum activity was observed against E. coli, B. Subtilisand,C.albican and C.glabrata at 40mg/ml. Agar well diffusion assay was used to determine minimum inhibitory concentration of all test microorganisms.

scholarly journals Antimicrobial activity of leaf extracts of Euphorbia paralias L. and Melilotus sulcatus Desf. against some pathogenic microorganisms

2021 ◽  
pp. 10-15
Author(s):  
Miloud M. Miloud ◽  
Najma A. Senussi
Keyword(s):  
Antimicrobial Activity ◽  
Plant Extracts ◽  
Methanolic Extract ◽  
Bacterial Species ◽  
Fungal Species ◽  
Leaf Extracts ◽  
Methanolic Extracts ◽  
Bioactive Agents ◽  
Agar Well Diffusion Assay ◽  
Diffusion Assay

The present study was aimed to investigate the antimicrobial potential of leaf extracts of Euphorbia paralias and Melilotus sulcatus against four bacterial species Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Klebsiella sp. and two fungal species Asperigillus niger and Aspergillus flavus. The agar well diffusion assay was used to evaluate the antimicrobial activity. The effect of these extracts was most effective against the bacterial species compared to the fungal species at a used concentration (100 mg/ml). Methanolic extracts of selected plants displayed good antimicrobial activity against all tested microorganisms species, while, no activity for aqueous extracts against tested fungal species. Methanolic extracts were the most effective plant extracts against all tested bacterial species, with MIC and MBC reached 6.2 and 12.5 mg/ml, except Klebsiella sp. which was less sensitive to M. sulcatus methanolic extract and its MIC and MBC reached 12.5 and 25 mg/ml, respectively. These plant extracts which proved to be potentially effective can be used as bioactive agents to control microorganisms caused for diseases and they can be used naturally in the human and veterinary healthcare systems.

scholarly journals Antimicrobial, Antioxidant and Cytotoxic Activities and Phytochemical Screening of Some Yemeni Medicinal Plants

2010 ◽  
Vol 7 (3) ◽  
pp. 323-330 ◽  
Author(s):  
Ramzi A. A. Mothana ◽  
Salah A. A. Abdo ◽  
Sidgi Hasson ◽  
Faisal M. N. Althawab ◽  
Sama A. Z. Alaghbari ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Cytotoxic Activity ◽  
Hot Water ◽  
Diffusion Method ◽  
Scavenging Activity ◽  
Phytochemical Screening ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Methanolic Extracts ◽  
Multiresistant Bacteria

The traditional medicine still plays an important role in the primary health care in Yemen. The current study represents the investigation of 16 selected plants, which were collected from different localities of Yemen. The plants were dried and extracted with two different solvents (methanol and hot water) to yield 34 crude extracts. The obtained extracts were tested for their antimicrobial activity against three Gram-positive bacteria, two Gram-negative bacteria, one yeast species and three multiresistantStaphylococcusstrains using agar diffusion method, for their antioxidant activity using scavenging activity of DPPH radical method and for their cytotoxic activity using the neutral red uptake assay. In addition, a phytochemical screening of the methanolic extracts was done. Antibacterial activity was shown only against Gram-positive bacteria, among them multiresistant bacteria. The highest antimicrobial activity was exhibited by the methanolic extracts ofAcalypha fruticosa,Centaurea pseudosinaica,Dodonaea viscosa,Jatropha variegata,Lippia citriodora,Plectranthus hadiensis,Tragia pungensandVerbascum bottae.Six methanolic extracts especially those ofA. fruticosa,Actiniopteris semiflabellata,D. viscosa,P. hadiensis,T. pungensandV. bottaeshowed high free radical scavenging activity. Moreover, remarkable cytotoxic activity against FL-cells was found for the methanolic extracts ofA. fruticosa,Iris albicans,L. citriodoraandT. pungens. The phytochemical screening demonstrated the presence of different types of compounds like flavonoids, terpenoids and others, which could be responsible for the obtained activities.

scholarly journals Screening of antimicrobial potential and brine shrimp lethality bioassay of the whole plant extract of Spilanthes paniculata Wall. ex DC

2015 ◽  
Vol 3 (1) ◽  
pp. 1-5
Author(s):  
Rokeya Siddiqui ◽  
Md Masud Alam ◽  
Mohammad Ruhul Amin ◽  
AFM Shahid Ud Daula ◽  
MM Hossain
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Crude Extract ◽  
Brine Shrimp ◽  
Methanolic Extract ◽  
Brine Shrimp Lethality ◽  
Whole Plant ◽  
Vincristine Sulphate ◽  
Soluble Fractions

Bangladesh possesses a rich flora of medicinal plant. Out of the estimated 5000 species of phanerogams and pteridophytes growing in this country, more than a thousand are regarded as having medicinal properties. The aim of this study was to evaluate the in vitro antimicrobial activity and brine shrimp lethality of the whole plant extracts of Spilanthes paniculata, a plant belonging to the family Asteraceae. The crude extract, n-Hexane, chloroform and ethyl acetate soluble fraction of crude extract showed significant to moderate antimicrobial activity against three Gram positive and three Gram negative microorganisms. The zones of inhibition produced by the crude extract (methanolic extract), n-hexane, choloform and ethyl acetate soluble fractions were found to be 14.89 mm-19.40 mm, 14 mm-19.40 mm, 10.66 mm-13.50 mm and 9.50 mm-13.26 mm, consecutively at a concentration of 30 µg/disc. N-Hexane, chloroform and ethyl acetate soluble fractions of methanolic extract of Spilanthes paniculata were screened for antitumor properties using brine shrimp lethality bioassay. From the results of the brine shrimp lethality bioassay, it can be well predicted that n-hexane, chloroform and ethyl acetate soluble fractions of methanolic extract possess mild cytotoxicity on shrimp naupalii. The median lethal concentration (LC50, the concentration at which 50% mortality of brine shrimp nauplii occurred) of n-hexane, chloroform and ethyl acetate were 48.978 mg/ml, 92.61 mg/ml and 216.770 mg/ml, respectively, comparison with positive control vincristine sulphate with 0.839 mg/ml. DOI: http://dx.doi.org/10.3329/sjm.v3i1.22743 Stamford Journal of Microbiology, Vol.3(1) 2013: 1-5

scholarly journals Bioactive Flavanones from Luma chequen

2002 ◽  
Vol 67 (1) ◽  
pp. 115-123 ◽  
Author(s):  
Cecilia Labbé ◽  
Josep Coll ◽  
Joseph D. Connolly ◽  
Francesca Faini ◽  
Louis J. Farrugia ◽  
...  
Keyword(s):  
Botrytis Cinerea ◽  
Brine Shrimp ◽  
Spodoptera Littoralis ◽  
Spectroscopic Data ◽  
Methanolic Extract ◽  
Chemical Study ◽  
Artemia Salina ◽  
X Ray

A bioassay-guided chemical study of a methanolic extract of fresh leaves of Luma chequen led to the isolation of lumaflavanones A (1), B (2) and C (3) whose structures are proposed on the basis of NMR spectroscopic data. The structure of lumaflavanone A was confirmed by X-ray analysis. Antifeedant (Spodoptera littoralis), brine shrimp (Artemia salina) and fungistatic (Botrytis cinerea) bioassays showed that while 3 was the most active in the first two assays the mixture of 1 and 2 was more effective as a fungistatic.

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