scholarly journals Antibacterial Activity of the Essential Oils of Pistacia lentiscus Used in Moroccan Folkloric Medicine

2011 ◽  
Vol 6 (10) ◽  
pp. 1934578X1100601 ◽  
Author(s):  
Fatima Zohra Mharti ◽  
Badiaa Lyoussi ◽  
Abdelfattah Abdellaoui

The essential oil of the leaves of Pistacia lentiscus, collected from the middle Atlas in Morocco, was analyzed by GC and GC–MS. Altogether 43 components in concentrations of more than 0.2% were identified representing 97.4% of the oil composition. The main constituents were germanicol (12.8%), thunbergol (8.8%), himachalene (7.4%), trans-squalene (6.7%), terpinyl propionate (6.7%), 3,3-dimenthol (6.2%) and cadina-1.4-diene (5.1%). The oils showed strong activity against Klebsiella pneumonia, but no activity against Pseudomonas aeruginosa.

2018 ◽  
Vol 16 (S1) ◽  
pp. S155-S163 ◽  
Author(s):  
S. Mehalaine ◽  
O. Belfadel ◽  
T. Menasria ◽  
A. Messaili

The present study was carried out to determine, for the first time, the chemical composition and antibacterial activity of essential oils derived from the aerial parts of three aromatic plants Thymus algeriensis Boiss & Reut, Rosmarinus officinalis L., and Salvia officinalis L. growing under semiarid conditions. The essential oils were chemically analyzed and identified by gas chromatography (GC) and GC/ mass spectrometry (GC/MS) and their antimicrobial activity was individually evaluated against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa using both agar disk diffusion and agar dilution methods. The major constituents of Thymus algeriensis essential oil were identified as camphor (13.62%), 1,8-cineol (6.00%), borneol (5.74%), viridiflorol (4.00%), and linalool (3.93%). For Rosmarinus officinalis essential oil, 48 compounds were characterized, of which the main constituents were camphor (17.09%), Z-β-ocimene (10.88%), isoborneol (9.68%), α-bisabolol (7.89%), and borneol (5.11%). While, Salvia officinalis essential oil was characterized by β-thujone (16.44%), followed by viridiflorol (10.93%), camphor (8.99%), 1,8-cineol (8.11%), trans-caryophyllene (5.85%), and α-humulene (4.69%) as the major components. Notably, results from antibacterial screening indicated that Thymus algeriensis and Salvia officinalis essential oils exhibited a strong inhibitory effect against both Escherichia coli and Staphylococcus aureus compared to Rosmarinus officinalis essential oil. Further, less activity was recorded against Pseudomonas aeruginosa for the three tested essential oils.


2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Lobna El-Hosseiny ◽  
Moustafa El-Shenawy ◽  
Medhat Haroun ◽  
Fadhil Abdullah

Gas chromatography/mass spectroscopy analysis was performed to identify the chemical components of three extracted essential oils including thyme, marjoram, and sage. The antibacterial activity of the extracted essential oils against Pseudomonas aeruginosa (ATCC 9027) was investigated using disc diffusion assay, either alone or in combination with standard antibiotics (piperacillin, cefepime, meropenem, gentamicin, and norfloxacin). Results showed that the studied oils exhibited a variety of activities against the tested bacterium. Thyme oil was the most active followed by marjoram oil, whereas sage displayed no activity towards the tested organism. Thyme oil enhanced the antibacterial activity of cell wall targeting antibiotics (piperacillin, cefepime, and meropenem) by more than twofold. Marjoram oil potentiated the activity of all the tested antibiotics except norfloxacin. Sage, despite its inactivity against pseudomonas, synergistically enhanced the activity of piperacillin, meropenem, and gentamicin. Thyme essential oil, containing thymol as a major component (33.6%), exhibited higher activity alone or in combination with antibiotics than marjoram which contained alcoholic terpenes or sage essential oil that contained 1,8-cineole as its major component (29%). The investigated oils, as natural bioactive agents, may be used to enhance the activity of antibiotics towards pseudomonas.


2005 ◽  
Vol 60 (1-2) ◽  
pp. 30-34 ◽  
Author(s):  
Ali Sonboli ◽  
Fereshteh Eftekhar ◽  
Morteza Yousefzadi ◽  
Mohammad Reza Kanani

The chemical composition of the essential oils obtained from two samples (GP1 and GP2) of Grammosciadium platycarpum Boiss. was analyzed by GC and GC-MS. The analysis of the oils resulted in the identification of twenty-two constituents. Linalool (79.0% - GP1, 81.8% - GP2) and limonene (10.0%, 5.8%) were found to be the major components, respectively. The in vitro antibacterial activities of these oils and their main compounds against seven Gram-positive and Gram-negative bacteria were investigated. The results exhibited that the total oils and their major components possess strong to moderate activities against all the tested bacteria except for Pseudomonas aeruginosa.


2011 ◽  
Vol 6 (5) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Janne Rojas ◽  
Alexis Buitrago ◽  
Luis Rojas ◽  
Antonio Morales ◽  
María Lucena ◽  
...  

The essential oil from the fruits of Vismia baccifera Triana & Planch. (Gutttiferae), collected in June 2009, was analyzed by GC/MS. A yield of 0.6% oil was obtained by hydrodistillation. Twenty-seven components were identified by comparison of their mass spectra with those in the Wiley GC-MS Library data base. The major components were trans-cadin-1,4-diene (36.6%), cis-cadin-1,4-diene (18.8%) and β-caryophyllene (11.9%). The essential oil showed a broad spectrum of antibacterial activity against the important human pathogenic Gram-positive and Gram-negative bacteria Staphylococcus aureus (ATCC 25923), Enterococcus faecalis (ATCC 29212), Escherichia coli (ATCC 25992), Pseudomonas aeruginosa (ATCC 27853) and Klebsiella pneumoniae (ATCC 23357) with MIC values ranging from 9 to 37 μg/mL.


INDIAN DRUGS ◽  
2018 ◽  
Vol 55 (06) ◽  
pp. 51-55
Author(s):  
N Belloula ◽  
◽  
S. Dridi ◽  
A Khattaf

In the present study, Cedrus atlantica leaves volatile oil were screened for their antibacterial activity and chemical composition. The essential oil chemical constituents obtained by hydro-distillation and analyzed by gas chromatography coupled with mass spectrometry (GC-MS). The antibacterial activity was tested in vitro on Gram-negative bacteria: Escherichia coli, Pseudomonas and Klebsiella pneumonia and Gram-positive: Staphylococcus aureus, Bacillus, and Enterococcus faecalis, using disc-diffusion method. Twenty five compounds were identified in leaves oil representing 66.0.9 % of the total oil composition. Cedrus atlantica essential oil yield was 1.64% and major compounds were α-pinene (8,65%), himachalene (8.41%), β-himachalene (8,14%), σ-himachalene (6,26%), cis-α-atlantone (5,11%), himachalol (4,25%) and α-himachalene (3,25%), germacrene D (3.15%), β-caryophyllene (2.14%), cadinene (2.00%), β-pinene (1.95%), humulene (1.20%) and β-copaene (1.16%). In both strain, Cedrus atlantica oil exhibited very considerable antibacterial activity.


2019 ◽  
Vol 6 (1) ◽  
pp. 10
Author(s):  
Dwi Rizki Febrianti ◽  
Yugo Susanto ◽  
Rakhmadhan Niah ◽  
Siti Latifah

ABSTRAK Jeruk siam yang berkembang di Kalimantan Selatan telah dikukuhkan menjadi varietas unggul nasional dengan nama jeruk siam Banjar. Kulit jeruk belum dimanfaatkan secara optimal hanya dibuang sebagai limbah. Kulit jeruk mengandung beberapa senyawa salah satunya mengandung senyawa aktif minyak atsiri. Tujuan penelitian ini adalah untuk mengetahui adanya aktivitas antibakteri minyak atsiri kulit jeruk siam Banjar (Citrus reticulata) terhadap pertumbuhan Pseudomonas aeruginosa. metode penarikan minyak atsiri kulit jeruk siam Banjar menggunakan metode destilasi air. Uji aktivitas antibakteri dilakukan dengan menggunakan metode difusi cakram terhadap bakteri Pseudomonas aeruginosa. Hasil penelitian menunjukkan bahwa  minyak atsiri kulit jeruk siam Banjar diperoleh sebanyak 10 mL (0,58%), berwarna kuning, aroma khas jeruk, bentuk cair, rasa getir dan tidak ada noda transparan. Hasil penelitian uji aktivitas menunjukkan minyak atsiri kulit jeruk siam Banjar dapat menghambat pertumbuhan bakteri Pseudomonas aeruginosa. Pada volume 50 µL, 75 µL, dan 100 µL minyak atsiri kontrol positif (ciprofloxacin) sedangkan kontrol negatif (aqua pro injection). diameter zona bening disekitar cakam  dengan rata-rata diameter zona hambat yang dihasilkan beturut-turut 3,55 mm, 4,54 mm, 5,14 mm, 22,38 mm, dan 0 mm. Kata kunci: Kulit jeruk siam Banjar (Citrus reticulata), Minyak atsiri,  Pseudomonas aeruginosa. ABSTRACT Jeruk siam that develop in South Kalimantan have been confirmed as national superior varieties by the name of jeruk siam banjars. Citrus skin has not been used optimally only as waste. Orange peel contains several compounds, one of which contains active compounds of essential oils. The purpose of this study was to determine the antibacterial activity of essential oils of jerk siam banjars skin (Citrus reticulata) to the growth of Pseudomonas aeruginosa. method of withdrawal of jeruk siam banjars essential oil using a water distillation method. Antibacterial activity test was carried out using the disc diffusion method against the bacterium Pseudomonas aeruginosa. The results showed that 10 mL (0.58%) of essential oil from the jeruk siam banjars, yellow, orange aroma, liquid form, bitter taste, and no transparent stains. The results of the activity test showed that the essential oil of the skin of the jeruk siam banjars could inhibit the growth of the bacterium Pseudomonas aeruginosa. At the volume of 50 µL, 75 µL, and 100 µL of essential oil control positive (ciprofloxacin) while the negative control (aqua pro injection). the diameter of the clear zone around the paper disk with the average diameter of the inhibition zone produced was 3.55 mm, 4.54 mm, 5.14 mm, 22.38 mm and 0 mm respectively.Keywords: jeruk siam banjar (Citrus reticulata), essential oil, Pseudomonas aeruginosa.


2010 ◽  
Vol 5 (12) ◽  
pp. 1934578X1000501 ◽  
Author(s):  
Marianna Usai ◽  
Marzia Foddai ◽  
Barbara Sechi ◽  
Claudia Juliano ◽  
Mauro Marchetti

Thymus capitatus growing wild in Sardinia showed different essential oil composition if grown surrounding Cagliari than in north Sardinia. Here we verify the composition and antimicrobial activity of the oil to make it suitable for the cosmetic and confectionery industries. With the aim of improving the scent and the antimicrobical activity of T. capitatus essential oil, a hydroformylation reaction was carried out to transform the unsaturated components of the oil into the corresponding aldehydes. The essential oil of T. capitatus exhibited a significant antibacterial activity (MIC 0.125-0.5 mg/mL), and was also found effective on C. albicans (MIC 0.125 mg/mL). After hydroformylation, several new irregular terpenoid aldehydes were detected. The perfume of the new terpenic-like aldehydes is very agreeable and, therefore, the acceptability of the aroma is remarkably improved, but the antimicrobial activity was not increased.


2021 ◽  
Vol 29 (2) ◽  
pp. 73-77
Author(s):  
H. Fenghour ◽  
H. Bouabida ◽  
D. Dris ◽  
M. Houhamdi

Essential oils are secondary plant metabolites and have many therapeutic properties. The aim of our study is to determine the antibacterial effect of the essential oils of two plants cultivated in a semi-arid region located in the Northeast of Algeria (Tebessa), Eucalyptus camaldulensis (Myrtaceae) and Artemisia herba alba (Asteraceae). The yield of essential oils of the two plants were 1.45 ± 0.026 and 1.21 ± 0.061 g/100 g of the dry matter of the aerial part respectively. The test of the antibacterial effect is based on the diffusion method on solid medium (sensitivity), this method allows us to determine the susceptibility or resistance of an organism vis-à-vis the sample studied. Our study reveals that E. camaldulensis essential oil had very strong activity on all bacterial strains tested, except on Pseudomonas aeruginosa and Enterococcus faecalis for which there was no inhibitory effect. However, A. herba alba essential oil had very strong activity on all bacterial strains tested except on Pseudomonas aeruginosa. The MIC of Artemisia essential oil ranged between 0.08 and 1.57 µL/mL, with the lowest activity for S. aureus and P. mirabilis (1.57 µL/mL) and the highest activity was observed against E. feacalis, E. coli, and K. pneumonia (0.09 µL/mL). The MIC of the second plant EO ranged between 0.08 and 0.36 µL/mL, with the lowest activity for P. mirabilis (0.36 µL/mL) and the highest one was observed against S. saprophyticus and E. coli (0.08 µL/mL). Statistical analysis shows that the two plants have the same efficacy against S. saprophyticus while E. faecalis, K. pneumoniae and P. mirabilis species are affected more by the essential oil of A. herba alba. While, E. camaldulensis has a higher efficiency than that of A. herba alba on the species: S. aureus and E. coli. Therefore, the essential oils of E. camaldulensis and A. herba alba suggests avenues for further non clinical and clinical studies.


2019 ◽  
Vol 8 (5) ◽  
pp. 390-396
Author(s):  
Hannou Zerkani ◽  
Imane Tagnaout ◽  
Aziz Dirioiche ◽  
Imad Adadi ◽  
Jamila El Karkouri ◽  
...  

The objective of this study was to identify new bioactive substances through the phytochemical study and evaluation of the antibacterial activity of the essential oils of Tetraclinis articulata (Vahl). Harvested in April 2016, in the region of Khenifra (Middle Atlas-Morocco). Separate of hydrodistillation extracts of the three organs (branches, leaves and fruits) of Tetraclinis articulate were performed. The yields of essential oils are 0.92% for fruits; 0.41% for branches and 0.61% for leaves. The GC/MS analysis allowed the identification of 33 compounds in essential oil leaves, 20 compounds in excess fruits and 58 compounds in branches. The main compounds are bornyl acetate (38.54%) and α-pinene (6.71%) for leaves. α-pinene (22.12%); 13-epi-mannol (3.58%) and retinol (3.44%) for branches. Bornyl acetate (19.96%); α-pinene (12.47%) and retinol (11.98%) for fruits. The antibacterial properties of the essential oil of leaves of Tetraclinis articulata (Vahl) were tested in clinical bacterial strain. The essential oils inhibit the growth of Escherichia coli, Staphylococcus aureus and Klebsiella pneumoniae at 1.2 µl/ml; 2.4 µl/ml and 9.7 µl/ml respectively. Indeed, the resistance of bacteria to antibiotics is known for its adverse effects on human health, to escape this problem, it is necessary to exploit the antibiotics from aromatic and medicinal plants.


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