scholarly journals Effects of Anthocyanins and Melatonin From Purple Waxy Corn By-Products on Collagen Production by Cultured Human Fibroblasts

2019 ◽  
Vol 14 (7) ◽  
pp. 1934578X1986351 ◽  
Author(s):  
Thithima Rimdusit ◽  
Suthasinee Thapphasaraphong ◽  
Ploenthip Puthongking ◽  
Aroonsri Priprem
Keyword(s):  
Mass Spectrometry ◽  
High Performance ◽  
Zea Mays L ◽  
Human Fibroblasts ◽  
Ethanol Extract ◽  
Human Skin Fibroblasts ◽  
Collagen Production ◽  
Cultured Human Fibroblasts ◽  
Waxy Corn ◽  
By Products

Cob and silk of purple waxy corn ( Zea mays L. Ceratina Kulesh) are underutilized sources of anthocyanins, which could be extracted by various solvents: water, ethanol, methanol, 50% ethanol, and 50% methanol. Anthocyanin and melatonin levels were investigated. The quantifications of anthocyanins as cyanidin-3-glucoside, pelargonidin-3-glucoside, and peonidin-3-glucoside were determined by high-performance liquid chromatography combined with mass spectrometry (HPLC-MS/MS), and melatonin by HPLC-fluorescence. The ability of collagen production upon exposure to human skin fibroblasts from the different macerates of the cob and silk was also studied. All cob extracts showed higher level of anthocyanins than silk extracts. The 50% ethanol extract of cob showed the highest cyanidin-3-glucoside level at 2.42 ± 0.03 mg/g dried weight, whereas that of silk showed the highest content of cyanidin-3-glucoside at 1.95 ± 0.04 mg/g dried weight. Although cob extracts contained more anthocyanins than silk extracts, silk extracts could stimulate collagen production more than cob extracts. Therefore, the collagen production was likely due to the amount of melatonin in the silk extracts, which contained greater quantities than those of the cob, and the lipophilicity of melatonin or the hydrophilicity of anthocyanins also affected the ability of cell permeation. Thus, anthocyanins and melatonin levels should be considered for the biological activity study.

Insulin and Serum-stimulated Hexose Transport in In Vitro Aged Cultured Human Skin Fibroblasts

1986 ◽  
Vol 5 (3) ◽  
pp. 157-164 ◽  
Author(s):  
Ralph J. Germinario ◽  
Maureen Oliveira ◽  
Susannia Manuel
Keyword(s):  
Human Skin ◽  
Human Fibroblasts ◽  
Skin Fibroblasts ◽  
Proliferative Potential ◽  
Hexose Transport ◽  
Human Skin Fibroblasts ◽  
Short Term ◽  
Cultured Human Fibroblasts ◽  
Insulin Responses

ABSTRACTInsulin (0.67 μM) and serum (10% v/v) stimulated hexose transport did not change with in vitro ageing of cultured human skin fibroblasts. Additionally, dexamethasone amplification of the insulin-stimulated response which yielded responses to near physiological concentrations of insulin (i.e. 6.7 nM), showed no changes with in vitro ageing. Basal (i.e. nonstimulated) hexose transport, either in the presence or absence of dexamethasone, did not change with in vitro ageing. The in vitro loss of proliferative potential during in vitro ageing of cultured human fibroblasts probably does not involve changes in short term insulin responses (e.g. stimulation of hexose transport).

scholarly journals Anti-Obesity Effect of an Ethanol Extract of Cheongchunchal In Vitro and In Vivo

Nutrients ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 3453
Author(s):  
Hye Won Kawk ◽  
Gun-He Nam ◽  
Myeong Jin Kim ◽  
Sang-Yong Kim ◽  
Gi No Kim ◽  
...  
Keyword(s):  
High Performance ◽  
Adipocyte Differentiation ◽  
Ethanol Extract ◽  
Inhibitory Effect ◽  
Morphological Observation ◽  
Related Factors ◽  
Blood Biochemical ◽  
Waxy Corn

Cheongchunchal (CE) is a developed crop more highly enriched in cyanidin-3-O-glucoside chloride (anthocyanin) than conventional waxy corn. Anthocyanin has been proven to have anti-oxidant, anti-inflammatory, anti-obesity, and anti-cancer effects. In this study, using high-performance liquid chromatography (HPLC), Cheongchunchal was confirmed to contain 8.99 mg/g anthocyanin. The inhibitory effect of an ethanol extract of Cheongchunchal (CE) on adipocyte differentiation was demonstrated using Oil Red O staining and a triglyceride assay. By conducting Western blotting, we also confirmed the regulatory effect of CE on adipocyte differentiation factors by assessing changes in the levels of factors that play a significant role in the differentiation of 3T3-L1 preadipocytes. A C57BL/6N mouse model of obesity was induced with a high-fat diet, and CE (400, 600, and 800 mg/kg/day) or Garcinia (245 mg/kg/day) was orally administered to verify the anti-obesity effect of CE. As a result of CE administration, the food efficiency ratio (FER), weight gain, and weight of tissues decreased. Additionally, blood biochemical changes were observed. Furthermore, the inhibitory effect of CE on adipocytes was confirmed through morphological observation and the expression of adipocyte differentiation-related factors in the liver and fat tissues. Therefore, in this study, we verified the anti-obesity effects of anthocyanin-rich CE both in vitro and in vivo.

Identification of new lignans in Norway spruce knotwood extracts

Holzforschung ◽  
2012 ◽  
Vol 66 (5) ◽  
pp. 553-567 ◽  
Author(s):  
Annika I. Smeds ◽  
Iveta Češková ◽  
Patrik C. Eklund ◽  
Stefan M. Willför
Keyword(s):  
Mass Spectrometry ◽  
Norway Spruce ◽  
High Performance ◽  
Mass Spectra ◽  
Ethanol Extract ◽  
Dry Weight ◽  
Flash Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Resonance Spectroscopy ◽  
Hydrophilic Extract

Abstract In a hydrophilic extract of Norway spruce knotwood, the dominating lignan, 7-hydroxymatairesinol, was partially removed by precipitation, and the resulting mixture was fractionated by flash chromatography and preparative high-performance liquid chromatography (HPLC). In the HPLC fractions, 7S- and 7R-todolactol A, 7′-hydroxylariciresinol, and two stereoisomers of 9′-hydroxylariciresinol were identified by gas chromatography-mass spectrometry (GC-MS) analyses, and their structures were confirmed by nuclear magnetic resonance spectroscopy. The 9′-hydroxylariciresinols were suggested to have the 7S,8R,8′R,9′Rand 7R,8R,8′R,9′Rconfigurations, and (-)-7′-hydroxylariciresinol 7S*,7′R*,8R*,8′S*, which indicates a configuration of this structure that has not been reported previously. 7S- and 7R-isoliovil were identified by comparison with previously published GC-MS data, and 7′-oxolariciresinol was tentatively identified on the basis of its mass spectrum. Of these lignans, only 7′-hydroxylariciresinol has been identified previously in Norway spruce. Several other lignans with similar mass spectra as the todolactols, isoliovils, and 7′-hydroxylariciresinol were detected, indicating that they are different stereoisomers of these lignans and/or of liovils. In addition to these lignans, the mass spectra of several other unidentified minor lignans indicated the presence of several tens of previously unidentified minor lignans in Norway spruce knotwood, accounting altogether for approximately 6% of the dry weight of the ethanol extract. 7S-Todolactol A, which was dominating among the new lignans, was found to be very unstable in aqueous solutions. Identification of more of these unidentified lignans may be possible only by access to pure reference compounds.

scholarly journals Phenolic Compounds from Sesame Cake and Antioxidant Activity: A New Insight for Agri-Food Residues’ Significance for Sustainable Development

Foods ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 432 ◽  
Author(s):  
Reham Hassan Mekky ◽  
Essam Abdel-Sattar ◽  
Antonio Segura-Carretero ◽  
María del Mar Contreras
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
High Performance ◽  
Relevant Literature ◽  
Reversed Phase ◽  
Total Phenol Content ◽  
Nitrogenous Compounds ◽  
Food Residues ◽  
By Products

Agri-food residues represent a rich source of nutrients and bioactive secondary metabolites, including phenolic compounds. The effective utilization of these by-products in food supplements and the nutraceuticals industry could provide a way of valorization in the transition to becoming more sustainable. In this context, the present study describes the phenolic profiling of sesame (Sesamum indicum L.) cake using reversed-phase high-performance liquid chromatography coupled to diode array detection and quadrupole-time-of-flight-mass spectrometry. Compounds were characterized based on their retention time, UV spectra, accurate mass spectrometry (MS) and MS/MS data along with comparison with standards, whenever possible, and the relevant literature. The characterized compounds (112 metabolites) belong to several classes, namely, phenolic acids (hydroxybenzoic acids and hydroxycinnamic acids), flavonoids, and lignans. Moreover, organic acids and some nitrogenous compounds were characterized. The total phenol content and the antioxidant activity of the cake extract were determined. This study provides useful information for the valorization of by-products from the sesame oil industry.

scholarly journals Evaluation of antioxidant and anti-inflammatory effects of three different Rubus coreanus Miq. by-products

2021 ◽  
Vol 20 (11) ◽  
pp. 2317-2323
Author(s):  
Sung-Gyu Lee ◽  
Hyun Kang
Keyword(s):  
High Performance ◽  
Inflammatory Diseases ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Total Flavonoid ◽  
Added Value ◽  
Total Polyphenol ◽  
Rubus Coreanus ◽  
Anti Inflammatory ◽  
By Products

Purpose: To investigate the antioxidant and anti-inflammatory properties of three different Rubus coreanus Miq. by-products in stimulated BV-2 microglial cells and explore its underlying physiological efficacy.Methods: Cell viability assessment was performed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Lipopolysaccharide (LPS) was used to activate BV-2microglia. Total polyphenol and total flavonoid contents were determined by the method of Folin-Denis. As three different Rubus coreanus Miq. by-products remaining after extraction of Rubus coreanus, High performance liquid chromatography (HPLC)) finger printing, ABST (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging assay, and NO (nitric oxide) inhibitory assay were performed.Results: Three different Rubus coreanus by-product extract did not exhibit any signs of cytotoxicity to BV-2 cells up to 100 μg/ml concentration (p < 0.5). The LPS-activated excessive release of NO in BV-2 cells was significantly inhibited by Rubus coreanus by-product extract (p < 0.5) at 500 μg/mL). Total polyphenol and total flavonoid contents were highest in 50 % ethanol wine processing by-product (p < 0.5 at 30, 50, 70 and 100 %, respectively). The by-product of wine processing had the lowest RC50 radical scavenging effect (16.53 μL/ml). The quercetin content of the wine processing by-product was the highest in the 70% ethanol extract at 6.26 mg/g (p < 0.5 at 30, 50, 70 and 100%, respectively).Conclusion: These results reveal that of the three other by-products, wine processing by-product has the highest antioxidant and anti-inflammatory activities. The use of these by-products has high added value for industrial production; furthermore, they are a potential treatment for various inflammatory diseases.

scholarly journals Comparative chemical profiling of purified and unpurified Strychnos nux-vomica Linn seeds: An attempt to reduce toxic Brucine content

2021 ◽  
Vol 12 (4) ◽  
pp. 807-819
Author(s):  
Ajay Kumar Meena ◽  
Poorna Venktaraman ◽  
Panda P ◽  
Murali Krishna C ◽  
Kusuma Ganji ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance ◽  
Quantitative Estimation ◽  
Ethanol Extract ◽  
Chloroform Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Chemical Profiling ◽  
Chromatography Mass Spectrometry ◽  
Before And After ◽  
Layer Chromatography

Vishamusthi (Strychnos nux-vomica Linn.), a medicinal plant described as Upavisha (semi-poisonous) group of Ayurvedic Pharmacopoeia of India. Vishamusthi has widely been used and being practiced in several illness namely nervous debility, paralysis, weakness of limbs, sexual weakness, dyspepsia and etc. Ayurveda practices strictly recommend the use of Vishamusthi in therapeutics only after proper shodhana (purificatory procedure) through specific medias such as Gomutra (cow’s urine), Godugdha (cow’s milk), Goghrita (cow’s ghee), and etc. Although various shodhana procedures are recommended in Ayurvedic treatise, but updated scientific researches regarding the shodhana methods are lacking. The present study was undertaken to investigate the physicochemical and phytochemical parameters, quantitative estimation of brucine using cutting edge research tools such as high-performance thin layer chromatography (HPTLC), liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS) analyses of Vishamusthi seeds before and after purification. The remarkable changes have been observed in different physicochemical parameters, HPTLC, HPLC, GC-MS and LC-MS chromatographic profiling before and after shodhana process of Vishamusthi seeds. Quantitative HPLC studies revealed that the process of shodhana resulted in depletion of toxic brucine (chief poisonous constituent of Vishamusthi seeds) reduced to 79.66% in chloroform extract and 64.54% in ethanol extract after shodhana process.

Sign in / Sign up

Export Citation Format

Share Document