scholarly journals Two Cosmetic Properties of an Ethanol Extract of a Cultured and Edible Red Macroalga, Bangia fuscopurpurea: Moisturizing and Whitening Effects

2020 ◽  
Vol 15 (8) ◽  
pp. 1934578X2094466
Author(s):  
Shi-Ying Huang ◽  
Hui-Min David Wang ◽  
Jianhua Ke ◽  
Jian Li ◽  
Lili Chen ◽  
...  
Keyword(s):  
Moisture Absorption ◽  
Ethanol Extract ◽  
Positive Control ◽  
Total Phenolic ◽  
Red Macroalga ◽  
External Behavior ◽  
Bangia Fuscopurpurea

Previous studies have focused on the role of a cultured red macroalga Bangia fuscopurpurea as a functional food; however, except for antioxidant activity, there are no reports directly regarding the potential cosmetic properties of this alga. Our present study explored the moisturizing effect of its ethanol extract (BFH1) and used the tyrosinase activity inhibition assay to evaluate its in vitro whitening effect. The in vitro moisture-retention ability of BFH1 was similar to that of glycerol (positive control), but its moisture-absorption ability was significantly higher. The overall in vivo moisturizing effect of topical application of BFH1 in mice was similar to that of glycerol, but BFH1 did not cause significant changes in the oil content of the skin, and there were no obvious side effects regarding skin appearance and external behavior during treatment. BFH1 exerted in vitro tyrosinase inhibitory activity with a half-maximal inhibitory concentration (IC50) of 48.3 μg/mL (IC50 of positive control, vitamin C: 19.6 μg/mL). The total phenolic content of BFH1 was determined as 10.8 % ± 0.07 %. Thus, BFH1 has high potential to be turned into a cosmetic ingredient with moisturizing and whitening effects.

scholarly journals In vivo hypotensive effect and in vitro inhibitory activity of some Cyperaceae species

2013 ◽  
Vol 49 (4) ◽  
pp. 803-809
Author(s):  
Monica Lacerda Lopes Martins ◽  
Henrique Poltronieri Pacheco ◽  
Iara Giuberti Perini ◽  
Dominik Lenz ◽  
Tadeu Uggere de Andrade ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Colorimetric Assay ◽  
Hypotensive Effect ◽  
Ace Inhibition ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Total Phenolic ◽  
Before And After

In 1820, French naturalist August Saint Hillaire, during a visit in Espírito Santo (ES), a state in southeastern Brazil, reported a popular use of Cyperaceae species as antidote to snake bites. The plant may even have a hypotensive effect, though it was never properly researched. The in vitro inhibitory of the angiotensin converting enzyme (ACE) activity of eigth ethanolic extracts of Cyperaceae was evaluated by colorimetric assay. Total phenolic and flavonoids were determined using colorimetric assay. The hypotensive effect of the active specie (Rhychonospora exaltata, ERE) and the in vivo ACE assay was measured in vivo using male Wistar Kyoto (ERE, 0.01-100mg/kg), with acetylcholine (ACh) as positive control (5 µg/kg, i.v.). The evaluation of ACE in vivo inhibitory effect was performed comparing the mean arterial pressure before and after ERE (10 mg/kg) in animals which received injection of angiotensin I (ANG I; 0,03, 03 and 300 µg/kg, i.v.). Captopril (30 mg/kg) was used as positive control. Bulbostylis capillaris (86.89 ± 15.20%) and ERE (74.89 ± 11.95%, ERE) were considered active in the in vitro ACE inhibition assay, at 100 µg/mL concentration. ACh lead to a hypotensive effect before and after ERE's curve (-40±5% and -41±3%). ERE showed a dose-dependent hypotensive effect and a in vivo ACE inhibitory effect. Cyperaceae species showed an inhibitory activity of ACE, in vitro, as well as high content of total phenolic and flavonoids. ERE exhibited an inhibitory effect on both in vitro and in vivo ACE. The selection of species used in popular medicine as antidotes, along with the in vitro assay of ACE inhibition, might be a biomonitoring method for the screening of new medicinal plants with hypotensive properties.

scholarly journals The Ethanol Extract of Avocado (Persea americana Mill. (Lauraceae)) Seeds Successfully Induces Implant Regression and Restores Ovarian Dynamic in a Rat Model of Endometriosis

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Stéphane Minko Essono ◽  
Marie Alfrede Mvondo ◽  
Esther Ngadjui ◽  
François Xavier Kemka Nguimatio ◽  
Pierre Watcho
Keyword(s):  
Ethanol Extract ◽  
Serum Levels ◽  
Positive Control ◽  
Negative Control ◽  
Persea Americana ◽  
Corpora Lutea ◽  
Serum Samples ◽  
Female Wistar Rats

Endometriosis is an estrogen-dependent disease with conventional therapies which do not have desirable effectiveness and possess many side effects. Scientific evidences suggest that medicinal plants with antioxidant, anti-inflammatory, and/or antiproliferative properties are potential alternatives for the treatment of endometriosis. The ethanol extract of Persea americana Mill. (Lauraceae) seeds was found exhibiting antiproliferative properties in vitro and in vivo. This study therefore is aimed at investigating the effects of such an extract on an experimental model of endometriosis. Endometriosis was induced by grafting uterine fragments onto the peritoneum of female Wistar rats. After checking the success of the transplantation surgery, animals with endometriosis were orally treated with the ethanol extract of P. americana seeds at the doses of 12.5, 25, and 50 mg/kg. The positive control was treated with letrozole (10 mg/kg) while the negative control received the vehicle. Treatments lasted 7 days and animals were sacrificed thereafter. Endometrial implant volume was determined. Estradiol and progesterone levels were measured in serum samples and endometriosis lesions. The oxidative status of endometriosis lesions was evaluated. Histological analysis of endometriosis lesions, uterus, and ovaries was also performed. Results showed that the ethanol extract of P. americana seeds decreased endometrial implant volume (p<0.001) and serum levels of estradiol and progesterone (p<0.01). The levels of estradiol also decreased in endometriosis lesions at doses of 12.5 and 50 mg/kg (p<0.001). Both malondialdehyde and glutathione levels increased in endometriosis lesions (p<0.001). The ectopic endometrium height decreased and the number of antral follicles and corpora lutea (p<0.05) increased while that of luteinized unruptured follicles decreased (p<0.001). In conclusion, the ethanol extract of P. americana seeds displayed an antiendometriosis effect suggesting that it could be a potential alternative for the treatment of endometriosis.

scholarly journals Anti-Obesity Effect of Extract from Nelumbo Nucifera L., Morus Alba L., and Raphanus Sativus Mixture in 3T3-L1 Adipocytes and C57BL/6J Obese Mice

Foods ◽  
2019 ◽  
Vol 8 (5) ◽  
pp. 170 ◽  
Author(s):  
Wan-Sup Sim ◽  
Sun-Il Choi ◽  
Bong-Yeon Cho ◽  
Seung-Hyun Choi ◽  
Xionggao Han ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Raphanus Sativus ◽  
Ethanol Extract ◽  
Morus Alba ◽  
Nelumbo Nucifera ◽  
Total Phenolic ◽  
Control Group ◽  
High Fat

The antioxidant and anti-adipogenic activities of a mixture of Nelumbo nucifera L., Morus alba L., and Raphanus sativus were investigated and their anti-obesity activities were established in vitro and in vivo. Among the 26 different mixtures of extraction solvent and mixture ratios, ethanol extract mixture no. 1 (EM01) showed the highest antioxidant (α,α-Diphenyl-β-picrylhydrazyl, total phenolic contents) and anti-adipogenic (Oil-Red O staining) activities. EM01 inhibited lipid accumulation in 3T3-L1 adipocytes compared to quercetin-3-O-glucuronide. Furthermore, body, liver, and adipose tissue weights decreased in the high-fat diet (HFD)-EM01 group compared to in the high-fat diet control group (HFD-CTL). EM01 lowered blood glucose levels elevated by the HFD. Lipid profiles were improved following EM01 treatment. Serum adiponectin significantly increased, while leptin, insulin growth factor-1, non-esterified fatty acid, and glucose significantly decreased in the HFD-EM01 group. Adipogenesis and lipogenesis-related genes were suppressed, while fat oxidation-related genes increased following EM01 administration. Thus, EM01 may be a natural anti-obesity agent.

scholarly journals IN VITRO AND IN VIVO STUDIES OF ANTIHYPERURICEMIC AND ANTIOXIDANT ACTIVITY FROM BULBS OF BAWANG TIWAI (ELEUTHERINE PALMIFOLIA (L.) MERR.) FROM INDONESIA

2019 ◽  
Vol 12 (1) ◽  
pp. 497
Author(s):  
Dian Ratih Laksmitawati ◽  
Rininta Firdaus ◽  
Mediana Astika Zein
Keyword(s):  
Antioxidant Activity ◽  
Body Weight ◽  
Uric Acid ◽  
Ethanol Extract ◽  
Positive Control ◽  
In Vivo Studies ◽  
Inhibit Lipid Peroxidation ◽  
Plasma Malondialdehyde

Objectives: This study would like to investigate the in vitro antioxidant activity through 2,2-diphenyl-1-picrylhydrazyl assay and in vitro xanthine oxidase activity of the bulbs. This study performs in vivo assays to study the antihyperuricemic activity and antioxidant in the hyperuricemic rat through plasma malondialdehyde measurement. Method: The study was conducted by testing the fresh bulbs of bawang tiwai (Eleutherine palmifolia (L.) Merr. with chemical solvent of ethanol 70% to extract the bulbs. Allopurinol and Vitamin C were used as positive control for the antihyperuricemic assay and antioxidant assay, respectively. Other chemical substances were also used in this study. This study used chicken extract (Brands) 20 ml/kg/body weight to induce the level of uric acid in the blood serum, and potassium oxonate (Sigma 156124) to inhibit the uricase in rats. Results: The results show that the levels of uric acid were measured using spectrophotometer with dichloro-hydroxybenzen sulfonate (Biolabo) a as reagent. The ethanol extract of bawang tiwai (EBT) (E. palmifolia (L.) Merr) was potential to reduce uric acid level at 140, 280, and 560 mg/kg body weight, but possibly without inhibition against xanthine oxydase activity. Conclusion: All doses of EBT could inhibit lipid peroxidation in hyperuricemic condition caused by high purine diet in 14 days.

scholarly journals COMPARATIVE STUDIES OF PHYTOCHEMICAL AND ANTIOXIDANT ACTIVITY OF IN VIVO PLANT AND IN VITRO CALLUS EXTRACT OF CARDIOSPERMUM HALICACABUM L.

2021 ◽  
pp. 94-103
Author(s):  
THANIARASU R ◽  
LOGESHWARI M
Keyword(s):  
Antioxidant Activity ◽  
Biological Activities ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Dry Weight ◽  
Biologically Active ◽  
Diffusion Method ◽  
Total Phenolic

Objective: The present investigation focuses on the use of Cardiospermum halicacabum L. in their phytochemical and biological activities. Methods: In this study, in vivo stem and in vitro callus ethanolic extracts of C. halicacabum were tested for their phytochemical attributes by qualitative method, Fourier transform infrared (FTIR), antioxidant, antibacterial, and bioactive compound properties. The bactericidal activity of the in vivo stem and in vitro callus extract has been evaluated in both Gram+ve and Gram-ve microorganisms using the disk diffusion method. Results: The highest frequency (78%) of well developed, dark green organogenic callus was induced from stem explant on Murashige and Skoog (MS) medium supplemented with 0.7 mg/l 2,4-Dichlorophenoxyacetic acid (2, 4-D) and 0.5 mg/l benzyl adenine (BA). The results of FTIR spectra confirmed the presence of functional groups in wild stem and in vitro callus extract of C. halicacabum with various peaks. The total phenolic content in ethanolic extract of in vivo plant and in vitro callus was 80.46 mg gallic acid equivalent (GAE)/g dry weight and 76.4 mg GAE/g dry weight, respectively. The highest percentage of tannins was measured at 78.03 in wild stem ethanol extracts followed by 75.22 in callus extract. The antioxidant activity of 2,2-diphenyl-2- picrylhydrazyl (DPPH) ethanol extract was found to be 206.54 μg/ml. IC50 values of the stem extracts of C. halicacabum are 306 μg/ml and 286 μg/ml in callus extract, respectively. Antibacterial activity of the ethanol extract was higher for Staphylococcus aureus (S. aureus) with a 17 mm zone of inhibition. Conclusion: The present investigation recommended that the callus ethanolic extract function as a good source of biologically active compounds and natural antioxidants.

scholarly journals Aktivitas antibakteri salep ekstrak etanol daun sirih hijau (Piper betleL.) Terhadap infeksi bakteri Staphylococcus aureus

2018 ◽  
Vol 5 (2) ◽  
pp. 56
Author(s):  
Rissa Laila Vifta ◽  
Muhammad Andri Wansyah ◽  
Anita Kumala Hati
Keyword(s):  
Staphylococcus Aureus ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Piper Betle ◽  
In Vitro Test ◽  
In Vivo Test ◽  
Betel Leaf

<p align="center"><strong>Abstrak </strong></p><p align="center"><strong> </strong></p><p>Sirih hijau (<em>Piper betle L</em>.) adalah tanaman yang mudah dijumpai di Indonesia. Secara empiris, sirih hijau  digunakan sebagai antibakteri pada luka. Penelitian ini bertujuan untuk mengetahui aktivitas salep ekstrak etanol daun sirih hijau sebagai antibakteri. Pengujian efek antibakteri dilakukan secara <em>in vitro </em>dan <em>in vivo</em>. Ekstrak etanol daun sirih hijau dibuat dengan cara maserasi. Pengujian secara <em>in vitro</em> dilakukan menggunakan metode mikrodilusi terhadap <em>Staphylococcus aureus</em>  untuk mengetahui nilai konsentrasi hambat minimum ekstrak daun sirih hijau pada konsentrasi 3, 4, dan 5%.  Kemudian ekstrak dibuat salep dan diuji secara <em>in vivo</em> menggunakan hewan uji tikus yang terdiri dari kelompok kontrol negatif, kelompok ekstrak (konsentrasi 3,4, dan 5%), serata pembanding kontrol positif gentamisin 0,1%. Hasil uji dianalisis menggunakan <em>Sapiro-Wilk</em> dan dilanjutkan dengan uji ANAVA serta uji LSD untuk mengetahui perbandingan hasil kelima kelompok perlakuan. Hasil uji <em>in vitro</em> menunjukkan bahwa konsentrasi hambat minimum ekstrak daun sirih yang optimal adalah 5%. Hasil uji <em>in vivo</em> menunjukkan lama kesembuhan luka meningkat seiring dengan bertambahnya konsentrasi ekstrak daun sirih hijau. Hasil uji normalitas, Anava, dan uji LSD pada uji <em>in vivo</em> memberikan hasil yang sejalan. Konsentrasi 4 dan 5% merupakan konsentrasi efektif dengan aktivitas waktu kesembuhan luka tidak berbeda bermakna dengan kontrol positif (gentamisin 0,1%) dengan rerata waktu berturut-turut 6,20±0,80 dan 6,00±0,71 hari. Dapat disimpulkan bahwa hasil uji antibakteri pada salep sejalan dengan uji secara <em>in-vitro</em> ekstrak daun Sirih Hijau yang memiliki potensi sebagai antibakteri.</p><p><strong>Kata kunci:</strong>     Daun sirih hijau, Antibakteri, Salep, Luka</p><p> </p><p align="center"><strong><em>Antibacterial activity from ointment contains extract of green betle leaves (Piper betle l.) to Staphylococcus aureus bacterial infection</em></strong></p><p align="center"><strong> </strong></p><p align="center"><strong><em>Abstract </em></strong></p><p align="center"><strong><em> </em></strong></p><p><em>Green betel (Piper betle L.) is a plant that is easy to find in Indonesia. Empirically, green betel is used as an antibacterial to the wound. This study aims to determine the activity of ointment </em><em>of </em><em>ethanol extract of green </em><em>betel</em><em> leaves as antibacterial. Tests of antibacterial effects were performed in vitro and in vivo. Ethanol extract of green betel leaf is made by maceration. In vitro testing was done using micro dilution method to Staphylococcus aureus to determine the minimum inhibitory concentration of green leaf extract at concentrations of 3, 4, and 5%. Then the extract was made ointment and tested in vivo using rat consisting of the negative control group, the extract group (concentrations 3,4, and 5%), the positive control of gentamicin 0.1%. The test results were analyzed using Sapiro-Wilk and continued with ANAVA test and LSD test to find out the comparison of the results of the five treatment groups. The results of in vitro test showed that the minimum inhibit concentration of </em><em>green </em><em>betel leaf extract was 5%. The results of in vivo test showed the duration of wound healing increased with increasing concentration of green betel leaf extract. The result of normality test, Anava, and LSD test on in vivo test give the same result. Concentrations of 4 and 5% were effective concentrations with wound healing time activity were not significantly different with positive control (gentamicin 0.1%) with mean time of 6.20 ± 0.80 and 6.00 ± 0.71 days respectively. It can be concluded that the antibacterial test results in ointment are in line with in vitro test of leaf </em><em>green betel </em><em>extract which has potential as antibacterial.</em><strong><em></em></strong></p><p><strong><em>Key words</em></strong><em>:      Green betel, Antibacterial, Ointment, Wound</em></p>

scholarly journals IN VITRO AND IN VIVO STUDIES OF ANTIHYPERURICEMIC AND ANTIOXIDANT ACTIVITY FROM BULBS OF BAWANG TIWAI (ELEUTHERINE PALMIFOLIA (L.) MERR.) FROM INDONESIA

2019 ◽  
Vol 12 (1) ◽  
pp. 497
Author(s):  
Dian Ratih Laksmitawati ◽  
Rininta Firdaus ◽  
Mediana Astika Zein
Keyword(s):  
Antioxidant Activity ◽  
Body Weight ◽  
Uric Acid ◽  
Ethanol Extract ◽  
Positive Control ◽  
In Vivo Studies ◽  
Inhibit Lipid Peroxidation ◽  
Plasma Malondialdehyde

Objectives: This study would like to investigate the in vitro antioxidant activity through 2,2-diphenyl-1-picrylhydrazyl assay and in vitro xanthine oxidase activity of the bulbs. This study performs in vivo assays to study the antihyperuricemic activity and antioxidant in the hyperuricemic rat through plasma malondialdehyde measurement. Method: The study was conducted by testing the fresh bulbs of bawang tiwai (Eleutherine palmifolia (L.) Merr. with chemical solvent of ethanol 70% to extract the bulbs. Allopurinol and Vitamin C were used as positive control for the antihyperuricemic assay and antioxidant assay, respectively. Other chemical substances were also used in this study. This study used chicken extract (Brands) 20 ml/kg/body weight to induce the level of uric acid in the blood serum, and potassium oxonate (Sigma 156124) to inhibit the uricase in rats. Results: The results show that the levels of uric acid were measured using spectrophotometer with dichloro-hydroxybenzen sulfonate (Biolabo) a as reagent. The ethanol extract of bawang tiwai (EBT) (E. palmifolia (L.) Merr) was potential to reduce uric acid level at 140, 280, and 560 mg/kg body weight, but possibly without inhibition against xanthine oxydase activity. Conclusion: All doses of EBT could inhibit lipid peroxidation in hyperuricemic condition caused by high purine diet in 14 days.

scholarly journals Aktivitas antibakteri salep ekstrak etanol daun sirih hijau (Piper betleL.) Terhadap infeksi bakteri Staphylococcus aureus

2017 ◽  
Vol 5 (2) ◽  
pp. 56
Author(s):  
Rissa Laila Vifta ◽  
Muhammad Andri Wansyah ◽  
Anita Kumala Hati
Keyword(s):  
Staphylococcus Aureus ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Piper Betle ◽  
In Vitro Test ◽  
In Vivo Test ◽  
Betel Leaf

<p align="center"><strong>Abstrak </strong></p><p align="center"><strong> </strong></p><p>Sirih hijau (<em>Piper betle L</em>.) adalah tanaman yang mudah dijumpai di Indonesia. Secara empiris, sirih hijau  digunakan sebagai antibakteri pada luka. Penelitian ini bertujuan untuk mengetahui aktivitas salep ekstrak etanol daun sirih hijau sebagai antibakteri. Pengujian efek antibakteri dilakukan secara <em>in vitro </em>dan <em>in vivo</em>. Ekstrak etanol daun sirih hijau dibuat dengan cara maserasi. Pengujian secara <em>in vitro</em> dilakukan menggunakan metode mikrodilusi terhadap <em>Staphylococcus aureus</em>  untuk mengetahui nilai konsentrasi hambat minimum ekstrak daun sirih hijau pada konsentrasi 3, 4, dan 5%.  Kemudian ekstrak dibuat salep dan diuji secara <em>in vivo</em> menggunakan hewan uji tikus yang terdiri dari kelompok kontrol negatif, kelompok ekstrak (konsentrasi 3,4, dan 5%), serata pembanding kontrol positif gentamisin 0,1%. Hasil uji dianalisis menggunakan <em>Sapiro-Wilk</em> dan dilanjutkan dengan uji ANAVA serta uji LSD untuk mengetahui perbandingan hasil kelima kelompok perlakuan. Hasil uji <em>in vitro</em> menunjukkan bahwa konsentrasi hambat minimum ekstrak daun sirih yang optimal adalah 5%. Hasil uji <em>in vivo</em> menunjukkan lama kesembuhan luka meningkat seiring dengan bertambahnya konsentrasi ekstrak daun sirih hijau. Hasil uji normalitas, Anava, dan uji LSD pada uji <em>in vivo</em> memberikan hasil yang sejalan. Konsentrasi 4 dan 5% merupakan konsentrasi efektif dengan aktivitas waktu kesembuhan luka tidak berbeda bermakna dengan kontrol positif (gentamisin 0,1%) dengan rerata waktu berturut-turut 6,20±0,80 dan 6,00±0,71 hari. Dapat disimpulkan bahwa hasil uji antibakteri pada salep sejalan dengan uji secara <em>in-vitro</em> ekstrak daun Sirih Hijau yang memiliki potensi sebagai antibakteri.</p><p><strong>Kata kunci:</strong>     Daun sirih hijau, Antibakteri, Salep, Luka</p><p> </p><p align="center"><strong><em>Antibacterial activity from ointment contains extract of green betle leaves (Piper betle l.) to Staphylococcus aureus bacterial infection</em></strong></p><p align="center"><strong> </strong></p><p align="center"><strong><em>Abstract </em></strong></p><p align="center"><strong><em> </em></strong></p><p><em>Green betel (Piper betle L.) is a plant that is easy to find in Indonesia. Empirically, green betel is used as an antibacterial to the wound. This study aims to determine the activity of ointment </em><em>of </em><em>ethanol extract of green </em><em>betel</em><em> leaves as antibacterial. Tests of antibacterial effects were performed in vitro and in vivo. Ethanol extract of green betel leaf is made by maceration. In vitro testing was done using micro dilution method to Staphylococcus aureus to determine the minimum inhibitory concentration of green leaf extract at concentrations of 3, 4, and 5%. Then the extract was made ointment and tested in vivo using rat consisting of the negative control group, the extract group (concentrations 3,4, and 5%), the positive control of gentamicin 0.1%. The test results were analyzed using Sapiro-Wilk and continued with ANAVA test and LSD test to find out the comparison of the results of the five treatment groups. The results of in vitro test showed that the minimum inhibit concentration of </em><em>green </em><em>betel leaf extract was 5%. The results of in vivo test showed the duration of wound healing increased with increasing concentration of green betel leaf extract. The result of normality test, Anava, and LSD test on in vivo test give the same result. Concentrations of 4 and 5% were effective concentrations with wound healing time activity were not significantly different with positive control (gentamicin 0.1%) with mean time of 6.20 ± 0.80 and 6.00 ± 0.71 days respectively. It can be concluded that the antibacterial test results in ointment are in line with in vitro test of leaf </em><em>green betel </em><em>extract which has potential as antibacterial.</em><strong><em></em></strong></p><strong><em>Key words</em></strong><em>:      Green betel, Antibacterial, Ointment, Wound</em>

scholarly journals Study the therapeutic role of Alcoholic Extract of Plantago lanceolata aganist infection with Staphylococcus saprophyticus

2012 ◽  
Vol 36 (0E) ◽  
pp. 8-15
Author(s):  
Aseel J. Mohammad
Keyword(s):  
Plantago Lanceolata ◽  
Kidney Tissue ◽  
Inhibition Zone ◽  
Positive Control ◽  
Control Group ◽  
Alcoholic Extract ◽  
Therapeutic Role

The present study was carried out to investigate the antibacterial activity of alcoholicextracts of Plantago lanceolata leafes in vitro and in vivo by inducing urinary tract infection inrats which caused by urethra administration of S.saprophyticus isolated from human andanimals(cow and sheep)These extracts showed significant effect (P<0.05) on the inhibition of the growth ofS.saprophyticus in vitro with the superiority of the concentration 200mg / ml of alcoholicextract with the mean of inhibition zone diameter 30 mm against S.saprophyticus ,while zonediameter was ( 26.5 ,21 ) mm due to the concentration 150, 100mg/ml respectively.This study included the therapeutic role of doses 150 mg/kg . B.W. of 1.5ml daily orally ofalcoholic extract dissolved in DMSO of plantago lanceolata leaves in the pathogenesis ofS.saprophyticus in rats by the urethral infection in compared with the control group (ratsinjected with S.saprophyticus without treatments).The results of histopathological changesshowed the role of Plantago lanceolata extract on the decreasing of pathological sings inbladder and kidney tissue after 14 and 21 days and gave negative results by decreasecongestion in the blood vessels of kidney hemorrhage and few infiltration of inflammatorycells in bladder , in compared with the positive control which showed acutehistopathological change.

Sign in / Sign up

Export Citation Format

Share Document