scholarly journals High-Efficiency and High-Throughput On-Chip Exchange of the Continuous Phase in Droplet Microfluidic Systems

2017 ◽  
Vol 22 (5) ◽  
pp. 529-535 ◽  
Author(s):  
Minkyu Kim ◽  
Chia Min Leong ◽  
Ming Pan ◽  
Lucas R. Blauch ◽  
Sindy K. Y. Tang
Keyword(s):  
High Throughput ◽  
Continuous Flow ◽  
High Efficiency ◽  
Scale Up ◽  
Continuous Phase ◽  
Microfluidic System ◽  
Droplet Surface ◽  
Generation Process ◽  
Microfluidic Systems ◽  
On Chip

This article describes an integrated platform for the on-chip exchange of the continuous phase in droplet microfluidic systems. The drops used in this work are stabilized by amphiphilic nanoparticles. For some characterizations and applications of these nanoparticle-stabilized drops, including the measurement of adsorption dynamics of nanoparticles to the droplet surface, it is necessary to change the composition of the continuous phase from that used during the droplet generation process. Thus far, no work has reported the exchange of the continuous phase for a large number (>1 million) of drops in a microfluidic system. This article describes the design and characterization of a high-efficiency and high-throughput on-chip exchanger of the continuous phase in a continuous-flow droplet microfluidic system. The efficiency of exchange was higher than 97%. The throughput was greater than 1 million drops/min, and this can be increased further by increasing the number of parallel exchangers used. Because drops are injected into the exchanger in a continuous-flow manner, the method is directly compatible with automation to further increase its reliability and potential scale-up.

scholarly journals Clarifying intact 3D tissues on a microfluidic chip for high-throughput structural analysis

2016 ◽  
Vol 113 (52) ◽  
pp. 14915-14920 ◽  
Author(s):  
Yih Yang Chen ◽  
Pamuditha N. Silva ◽  
Abdullah Muhammad Syed ◽  
Shrey Sindhwani ◽  
Jonathan V. Rocheleau ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Three Dimensional ◽  
Microfluidic System ◽  
High Throughput Drug Screening ◽  
Image Analysis Algorithm ◽  
Screening Assays ◽  
On Chip ◽  
Tissue Models

On-chip imaging of intact three-dimensional tissues within microfluidic devices is fundamentally hindered by intratissue optical scattering, which impedes their use as tissue models for high-throughput screening assays. Here, we engineered a microfluidic system that preserves and converts tissues into optically transparent structures in less than 1 d, which is 20× faster than current passive clearing approaches. Accelerated clearing was achieved because the microfluidic system enhanced the exchange of interstitial fluids by 567-fold, which increased the rate of removal of optically scattering lipid molecules from the cross-linked tissue. Our enhanced clearing process allowed us to fluorescently image and map the segregation and compartmentalization of different cells during the formation of tumor spheroids, and to track the degradation of vasculature over time within extracted murine pancreatic islets in static culture, which may have implications on the efficacy of beta-cell transplantation treatments for type 1 diabetes. We further developed an image analysis algorithm that automates the analysis of the vasculature connectivity, volume, and cellular spatial distribution of the intact tissue. Our technique allows whole tissue analysis in microfluidic systems, and has implications in the development of organ-on-a-chip systems, high-throughput drug screening devices, and in regenerative medicine.

scholarly journals The Fabrication and Application Mechanism of Microfluidic Systems for High Throughput Biomedical Screening: A Review

Micromachines ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 297 ◽  
Author(s):  
Kena Song ◽  
Guoqiang Li ◽  
Xiangyang Zu ◽  
Zhe Du ◽  
Liyu Liu ◽  
...  
Keyword(s):  
High Throughput ◽  
New Technologies ◽  
Biomedical Application ◽  
Raw Materials ◽  
Physical Structure ◽  
Microfluidic System ◽  
Microfluidic Chips ◽  
Microfluidic Systems ◽  
Microfluidic Technology

Microfluidic systems have been widely explored based on microfluidic technology, and it has been widely used for biomedical screening. The key parts are the fabrication of the base scaffold, the construction of the matrix environment in the 3D system, and the application mechanism. In recent years, a variety of new materials have emerged, meanwhile, some new technologies have been developed. In this review, we highlight the properties of high throughput and the biomedical application of the microfluidic chip and focus on the recent progress of the fabrication and application mechanism. The emergence of various biocompatible materials has provided more available raw materials for microfluidic chips. The material is not confined to polydimethylsiloxane (PDMS) and the extracellular microenvironment is not limited by a natural matrix. The mechanism is also developed in diverse ways, including its special physical structure and external field effects, such as dielectrophoresis, magnetophoresis, and acoustophoresis. Furthermore, the cell/organ-based microfluidic system provides a new platform for drug screening due to imitating the anatomic and physiologic properties in vivo. Although microfluidic technology is currently mostly in the laboratory stage, it has great potential for commercial applications in the future.

scholarly journals Microfluidic System for Observation of Bacterial Culture and Effects on Biofilm Formation at Microscale

Micromachines ◽  
2019 ◽  
Vol 10 (9) ◽  
pp. 606 ◽  
Author(s):  
Xiao-Yan Zhang ◽  
Kai Sun ◽  
Aliya Abulimiti ◽  
Pian-Pian Xu ◽  
Zhe-Yu Li
Keyword(s):  
Membrane Fouling ◽  
Continuous Flow ◽  
Biofilm Formation ◽  
Natural World ◽  
Microfluidic System ◽  
Fluorescence Labeling ◽  
In Situ Observation ◽  
Time Observation ◽  
On Chip ◽  
Bacteria Culture

Biofilms exist in the natural world and applied to many industries. However, due to the variety of characteristics caused by their complex components, biofilms can also lead to membrane fouling and recurrent infections which pose threats to human health. So, to make the best use of their advantages and avoid their disadvantages, knowing the best time and methods for improving or preventing biofilm formation is important. In situ observation without fluorescence labeling in microscale and according to a time scale is useful to research biofilm and confine its formation. In this study, we developed a microfluidic system for real-time observation of bacteria culture and biofilms development at microscale. We cultured E. coli ATCC 25922 on a chip at continuous flow of the velocity, which could promote bacterial formation. Biofilms formation under the condition of adding amoxicillin at different times is also discussed. In addition, the mixed strains from sludge were also cultured on chip, and possible factors in biofilm formation are discussed. Our results show that a microfluidic device could culture microorganisms in continuous flow and accelerate them to adhere to the surface, thereby promoting biofilm formation. Overall, this platform is a useful tool in research on initial biofilm formation, which can contribute to preventing biofouling and infections.

scholarly journals A droplet microfluidic platform for high-throughput photochemical reaction discovery

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Alexandra C. Sun ◽  
Daniel J. Steyer ◽  
Anthony R. Allen ◽  
Emory M. Payne ◽  
Robert T. Kennedy ◽  
...  
Keyword(s):  
High Throughput ◽  
Continuous Flow ◽  
High Throughput Screening ◽  
Resource Constraints ◽  
Scale Up ◽  
Industrial Process ◽  
Photochemical Reactions ◽  
Microfluidic Platform ◽  
Reaction Discovery ◽  
Compound Libraries

AbstractThe implementation of continuous flow technology is critical towards enhancing the application of photochemical reactions for industrial process development. However, there are significant time and resource constraints associated with translating discovery scale vial-based batch reactions to continuous flow scale-up conditions. Herein we report the development of a droplet microfluidic platform, which enables high-throughput reaction discovery in flow to generate pharmaceutically relevant compound libraries. This platform allows for enhanced material efficiency, as reactions can be performed on picomole scale. Furthermore, high-throughput data collection via on-line ESI mass spectrometry facilitates the rapid analysis of individual, nanoliter-sized reaction droplets at acquisition rates of 0.3 samples/s. We envision this high-throughput screening platform to expand upon the robust capabilities and impact of photochemical reactions in drug discovery and development.

scholarly journals On-chip density-based sorting of supercooled droplets and frozen droplets in continuous flow

Lab on a Chip ◽  
2020 ◽  
Vol 20 (21) ◽  
pp. 3876-3887
Author(s):  
Grace C. E. Porter ◽  
Sebastien N. F. Sikora ◽  
Jung-uk Shim ◽  
Benjamin J. Murray ◽  
Mark D. Tarn
Keyword(s):  
Continuous Flow ◽  
High Efficiency ◽  
Ice Nucleation ◽  
Continuous Separation ◽  
Microfluidic Platform ◽  
On Chip

We present a microfluidic platform for the continuous separation of frozen and unfrozen droplets based on differences in their density, allowing their sorting into different outlet channels with high efficiency towards applications in ice nucleation.

A scalable synthesis of ternary nanocatalysts for a high-efficiency electrooxidation catalysis by microfluidics

Nanoscale ◽  
2020 ◽  
Vol 12 (23) ◽  
pp. 12647-12654
Author(s):  
Yingyan Zhou ◽  
Dumei Wang ◽  
Xueming Kang ◽  
Dongtang Zhang ◽  
Xiangnan Dou ◽  
...  
Keyword(s):  
Continuous Flow ◽  
High Efficiency ◽  
Scale Up ◽  
High Dispersion ◽  
Nanomaterials Synthesis ◽  
Scalable Synthesis

An array-channel continuous flow scale-up strategy based on a microchip for high dispersion of loaded PtFeCu/C nanocatalysts for nanomaterials’ synthesis is reported.

scholarly journals Electropermanent magnet actuation for droplet ferromicrofluidics

TECHNOLOGY ◽  
2016 ◽  
Vol 04 (02) ◽  
pp. 110-119 ◽  
Author(s):  
José I. Padovani ◽  
Stefanie S. Jeffrey ◽  
Roger T. Howe
Keyword(s):  
Magnetic Fields ◽  
Water Droplet ◽  
Continuous Flow ◽  
Microfluidic System ◽  
Flow Conditions ◽  
Microfluidic Systems ◽  
Electromagnetic Actuation ◽  
On Demand ◽  
Current Pulses ◽  
Droplet Sorting

Droplet actuation is an essential mechanism for droplet-based microfluidic systems. On-demand electromagnetic actuation is used in a ferrofluid-based microfluidic system for water droplet displacement. Electropermanent magnets (EPMs) are used to induce 50 mT magnetic fields in a ferrofluid filled microchannel with gradients up to 6.4 × 104 kA/m2. Short 50 µs current pulses activate the electropermanent magnets and generate negative magnetophoretic forces that range from 10 to 70 nN on 40 to 80 µm water-in-ferrofluid droplets. Maximum droplet displacement velocities of up to 300 µm/s are obtained under flow and no-flow conditions. Electropermanent magnet-activated droplet sorting under continuous flow is demonstrated using a split-junction microfluidic design.

scholarly journals Microfluidic system for on-chip high-throughput whole-animal sorting and screening at subcellular resolution

2007 ◽  
Vol 104 (35) ◽  
pp. 13891-13895 ◽  
Author(s):  
C. B. Rohde ◽  
F. Zeng ◽  
R. Gonzalez-Rubio ◽  
M. Angel ◽  
M. F. Yanik
Keyword(s):  
High Throughput ◽  
Microfluidic System ◽  
On Chip ◽  
Subcellular Resolution

An Integrated Reservoir for On-Chip Aqueous Storage in Microfluidic Systems

1999 ◽  
Author(s):  
Manoj Patel ◽  
H. Thurman Henderson ◽  
Shekhar Bhansali ◽  
Chong H. Ahn
Keyword(s):  
Contact Surface ◽  
Lab On A Chip ◽  
Pressure Head ◽  
Microfluidic System ◽  
Design Issues ◽  
Microfluidic Systems ◽  
Initial Characterization ◽  
On Chip ◽  
Silicon Based

Abstract A reservoir for a silicon-based “lab-on-a-chip” integrated microfluidic system has been designed, fabricated and initially characterized. The reservoirs are necessary for storing reagents, antibodies and buffers required for on-chip capture of target microorganisms in this work. Aside from the matter of storing fluids and providing a pressure head for flow or flow augmentation one has the issue of biochemical compatibility of the contact surface. Where one does not desire a pressure head, a mere bio-chemically compatible “collapsible” bag is desired. All these factors are included in the device. This paper reports design issues, fabrication, packaging and initial characterization of the reservoirs. Scaling possibilities are essentially unlimited, however in this case standard reservoirs have been developed in modules of 1/8 and 1 ml capacity.

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