scholarly journals In Vitro Antioxidant Properties of Dichloromethanolic Leaf Extract of Gnidia glauca (Fresen) as a Promising Antiobesity Drug

2019 ◽  
Vol 24 ◽  
pp. 2515690X1988325 ◽  
Author(s):  
Wycliffe Arika ◽  
Cromwell Mwiti Kibiti ◽  
Joan Murugi Njagi ◽  
Mathew Piero Ngugi
Keyword(s):  
Oxidative Stress ◽  
Synergistic Effects ◽  
Antioxidant Properties ◽  
Iron Chelation ◽  
Reducing Power ◽  
Phytochemical Analysis ◽  
Traditional Practitioners ◽  
Antioxidant Effects ◽  
Hydroxyl Hydrogen

The acquisition of ethnobotanical information from traditional practitioners remains an empirical aspect of understanding the ethnopharmacology research. However, integration of information on chemical composition of plant extracts and their pharmacological activities forms a key resource for synthesis of new and effective therapeutics. In traditional African medicine, Gnidia glauca has folkloric remedies against obesity and its associated oxidative stress-mediated complications. However, the upsurge in its use has not been accompanied with scientific validations to support these claims. The present study aimed to determine the antioxidant potential of G glauca as a promising antiobesity agent. The antioxidant effects of the extract were assessed against 1,1-diphenyl-2-picrylhydrazyl, hydroxyl, hydrogen peroxide, nitric oxide, and superoxide radicals as well as lipid peroxidation, iron-chelating effect, and ferric-reducing power. Phytochemical analysis was conducted using gas chromatography linked to mass spectrophotometry. The results revealed that G glauca exhibited scavenging activities against all radicals formed. Besides, the extract showed iron chelation and ferric reducing abilities. The extract indicated a lower half maximal inhibitory concentration value than the standards used. For instance, the extract inhibited 50% of the formation of 2,2-diphenyl-1-picrylhydrazine at the concentration of 1.33 ± 0.03 mg/mL relative to 1.39 ± 0.06 mg/mL of the standard, vitamin C at 1% confidence limit. Similarly, the extract scavenged 50% of hydroxyl radical at 204.34 ± 10.64 μg/mL relative to 210.05 ± 8.80 μg/mL of gallic acid. The extract also contained various phytochemicals that have been associated with antiobesity effects. The synergistic effects of these phytocompounds increase their bioavailability and action on multiple molecular targets thereby correcting obesity-induced oxidative stress.

scholarly journals Phytochemical Analysis of Anti-Inflammatory and Antioxidant Effects of Mahonia aquifolium Flower and Fruit Extracts

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Andra-Diana Andreicut ◽  
Alina Elena Pârvu ◽  
Augustin Cătălin Mot ◽  
Marcel Pârvu ◽  
Eva Fischer Fodor ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Stress Index ◽  
Tnf Alpha ◽  
Phytochemical Analysis ◽  
Anti Inflammatory ◽  
Mahonia Aquifolium ◽  
Antioxidant Effects

Oxidative stress and inflammation are interlinked processes. The aim of the study was to perform a phytochemical analysis and to evaluate the antioxidant and anti-inflammatory activities of ethanolic Mahonia aquifolium flower (MF), green fruit (MGF), and ripe fruit (MRF) extracts. Plant extract chemical composition was evaluated by HLPC. A DPPH test was used for the in vitro antioxidant activity. The in vivo antioxidant effects and the anti-inflammatory potential were tested on a rat turpentine oil-induced inflammation, by measuring serum nitric oxide (NOx) and TNF-alpha, total oxidative status (TOS), total antioxidant reactivity (TAR), oxidative stress index (OSI), 3-nitrothyrosine (3NT), malondialdehyde (MDA), and total thiols (SH). Extracts were administrated orally in three dilutions (100%, 50%, and 25%) for seven days prior to inflammation. The effects were compared to diclofenac. The HPLC polyphenol and alkaloid analysis revealed chlorogenic acid as the most abundant compound. All extracts had a good in vitro antioxidant activity, decreased NOx, TOS, and 3NT, and increased SH. TNF-alpha was reduced, and TAR increased only by MF and MGF. MDA was not influenced. Our findings suggest that M. aquifolium has anti-inflammatory and antioxidant effects that support the use in primary prevention of the inflammatory processes.

Free Radical Scavenging potential of different extracts of Tabebuia roseo-alba (Ridl) Sand leaves

2021 ◽  
pp. 4801-4807
Author(s):  
Suseela V. ◽  
Sushmita L. ◽  
Bharatkumar R. ◽  
Nirmaladevi R.
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Free Radical Scavenging ◽  
Age Related ◽  
Radical Scavenging Properties ◽  
Hydroxyl Hydrogen

The present study was carried out to elucidate the in vitro free radical scavenging potential of various extracts of Tabebuia roseo-alba leaves. Assays for radical scavenging, such as DPPH, ABTS+, hydroxyl, hydrogen peroxide, superoxide, nitric oxide assay and reducing power activity were performed using standard protocols and the results were compared with standard ascorbic acid. Among the various extracts used the ethanolic extract of T. roseo-alba exhibited efficient scavenging potential with lowest EC50 value proving its antioxidant potential. Leaves of T. roseo-alba have strong free radical scavenging properties and thus can be used as a potential antioxidant to resolve diseases that are associated with oxidative stress including diabetes and other age related disorders.

scholarly journals Allium subhirsutum L. as a Potential Source of Antioxidant and Anticancer Bioactive Molecules: HR-LCMS Phytochemical Profiling, In Vitro and In Vivo Pharmacological Study

Antioxidants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1003
Author(s):  
Riadh Badraoui ◽  
Tarek Rebai ◽  
Salem Elkahoui ◽  
Mousa Alreshidi ◽  
Vajid N. Veettil ◽  
...  
Keyword(s):  
Pharmacological Study ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Bioactive Molecules ◽  
Skeletal Metastases ◽  
Total Phenolic ◽  
Walker 256 ◽  
Antioxidant Effects

This study investigated Allium subhirsutum L. (AS) anticancer and antioxidant effects and inhibition of tumor angiogenesis in a murine model of skeletal metastases due to inoculation of Walker 256/B cells. Phytochemical composition of AS extract (ASE) was studied by High Resolution-Liquid Chromatography Mass Spectroscopy (HR-LCMS). Total phenolic and flavonoid contents (TPC and TFC) were determined. In vitro, the antioxidant properties were evaluated by reducing power and antiradical activity against DPPH. Cancer cells’ proliferation, apoptosis, metastatic development and angiogenesis were evaluated using Walker 256/B and MatLyLu cells. The p-coumaric acid was the major phenolic acid (1700 µg/g extract). ASE showed high levels of TPC and TFC and proved potent antioxidant effects. ASE inhibited Walker 256/B and MatLyLu cells’ proliferation (Half-maximal inhibitory concentration: IC50 ≃ 150 µg/mL) and induced apoptosis. In silico and in vivo assays confirmed these findings. ASE effectively acts as a chemo-preventive compound, induces apoptosis and attenuates angiogenesis and osteolytic metastases due to Walker 256/B malignant cells.

scholarly journals PHYTOCHEMICAL PROFILING USING LC-Q-TOF-MS ANALYSIS AND IN VITRO ANTIOXIDANT ACTIVITY OF A RARE SALT-SECRETING MANGROVE AEGIALITIS ROTUNDIFOLIA ROXB. LEAVES EXTRACT

2019 ◽  
pp. 37-47
Author(s):  
Debjit Ghosh ◽  
Sumanta Mondal ◽  
Prof. Karipeddi Ramakrishna
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
In Vitro Models ◽  
Phytochemical Analysis ◽  
Ms Analysis ◽  
Aegialitis Rotundifolia ◽  
Tof Ms

Objective: The present work deals with the qualitative study of the phytoconstituents present in Aegialitis rotundifolia Roxb., ethanolic leaves extract and evaluate its antioxidant properties in vitro. Methods: The qualitative phytochemical analysis of the extract was performed first using preliminary phytochemical tests and then by liquid chromatography quadrupole-time-of-flight mass spectrometry (LC-Q-TOF-MS). The antioxidant properties were investigated comprehensively using seven in vitro models viz., 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, nitric oxide (NO) scavenging, hydrogen peroxide (H2O2) scavenging, superoxide (SOD) radical scavenging, lipid peroxidation (LPO) assay, reducing power (RP), and total antioxidant activity. Results: The preliminary phytochemical analysis revealed the presence of several important phytochemical groups whereas the LC-Q-TOF-MS analysis detected 25 phytoconstituents in the extract mostly belonging to flavonoids and alkaloids. The test extract showed strong dose-dependent antioxidant activity in all the seven in vitro models, however, the activity of the extracts was slightly lower compared to the reference standard ascorbic acid. Conclusion: The test extract showed strong antioxidant properties which could be possibly due to the phytoconstituents detected in the extract.

scholarly journals Antioxidant Properties of a Supercritical Fluid Extract of the Halophyte Mesembryanthemum nodiflorum L. from Sicilian Coasts: Nutraceutical and Cosmeceutical Applications

2020 ◽  
Vol 10 (7) ◽  
pp. 2374 ◽  
Author(s):  
Rosaria Arena ◽  
Simona Manuguerra ◽  
Edward Collins ◽  
Abdelkarim Mahdhi ◽  
Giuseppe Renda ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Supercritical Fluid ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Negative Effects ◽  
Cell Vitality ◽  
Aerial Parts ◽  
Bioactive Properties

The aim of this paper was to obtain different extracts from the aerial parts of Mesembryanthemum nodiflorum, comparing traditional extraction (using solvents with increasing polarity such as hexane, ethanol 80%, acetone 70% and water) with an eco-friendly technique (supercritical fluid extraction (SFE)); to evaluate which extract showed a higher amount of antioxidants and then evaluate the bioactive properties in vitro, in human fibroblast (HS68). From the six extracts obtained it was observed that the solvent with the highest extraction efficiency was water, but the extracts in ethanol, N-hexane and SFE are those that showed the highest antioxidant activity (polyphenols, 1,1-Diphenyl-2-picrylhydrazyl (DPPH) and reducing power). On the basis of these results, the SFE extract was chosen to evaluate the antioxidant and anti-aging activity in vitro, by assessing cell vitality and molecular markers (MTT and immunoblotting assays). The results showed that the SFE extract exerted antioxidant activity in vitro, protecting cells from mortality induced by oxidative stress; this protection was also confirmed at the molecular level, by the levels of the protein integrin -1, that is able to prevent the negative effects of a stress situation, such as oxidative stress, that could promote aging and related diseases. This extract, obtained with an eco-friendly technique, given its beneficial properties, could be used for application in nutraceuticals and cosmeceuticals.

scholarly journals Ethanolic Extract of Glycyrrhiza glabra to Ameliorate Oxidative Stress – Studies in vitro

2020 ◽  
Vol 2 (1) ◽  
pp. 80
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Glycyrrhiza Glabra ◽  
New Drugs ◽  
Phytochemical Analysis ◽  
Radical Scavenging Assay

Plants are major sources of bioactive organic molecules that are of interest to the pharmaceutical industry and are being screened for new drugs and chemicals. Glycyrrhiza glabra Linn. (Family: Fabaceae) also known as Liquorice, Mulaithi, or Yashtimadu is a well-known medicinal plant used in traditional medicine. Its roots and rhizomes are the medicinal parts used and are reported to possess antitumor, antimicrobial, antiviral, anti-inflammatory, immunoregulatory activities. This plant is also used as a flavoring agent due to its sweetness. In the present work, the ethanolic extract of Glycyrrhiza glabra was prepared, and its phytochemical analysis was done using HPLC. The in vitro antioxidant assays such as DPPH radical scavenging assay, Hydroxyl radical scavenging assay, total antioxidant activity assay, and total reducing power assay were done, and the results showed significant antioxidant activity of the extract. The extract was analyzed further to evaluate the ability to protect against oxidative stress in chicken liver tissue. The levels of glutathione and lipid peroxidation in H2O2 and/or G glabra extract-treated tissue indicated the potential to protect against oxidative stress under in vitro conditions. The in vitro comet assay results showed that the G glabra extract protected against H2O2 induced cellular DNA damage. These findings indicated promising antioxidant and antigenotoxic potential of G.glabra and need further exploration for translating these findings to its possible health benefits.

scholarly journals Lipid Nanoparticles Containing Mixtures of Antioxidants to Improve Skin Care and Cancer Prevention

Pharmaceutics ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 2042
Author(s):  
Catarina Gonçalves ◽  
Maria João Ramalho ◽  
Renata Silva ◽  
Vera Silva ◽  
Rita Marques-Oliveira ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cancer Prevention ◽  
Antioxidant Properties ◽  
In Vitro Release ◽  
Human Keratinocytes ◽  
Natural Compounds ◽  
Skin Care ◽  
Tem Analysis ◽  
Antioxidant Effects

Oxidative stress, triggered by UV radiation, is one of the major causes of free radical-associated disorders, such as skin cancer. The application of natural compounds (NCs) with antioxidant effects can attenuate free radicals’ accumulation and, therefore, provide a strategy for skin care and cancer prevention. In this work, three natural compounds, naringenin, nordihydroguaiaretic acid (NDGA), and kaempferol, were encapsulated into nanostructured lipid carriers (NLCs) aiming for the development of a formulation for cutaneous application with antioxidant properties. For the experiments, different formulation parameters were evaluated to optimize the NLCs that showed a diameter around 200 nm, which is an adequate particle size for incorporation in cosmetics. Transmission electron microscopy (TEM) analysis confirmed the NLCs’ typical spherical morphology. Encapsulation efficiency (EE) and loading capacity (LC) values revealed an effective production process, with EEs over 90% and LCs near the maximum value. The developed NLCs revealed a prolonged in vitro release of the natural compounds. The NLCs were stable under storage conditions, maintaining their psychochemical characteristics for 30 days. Additionally, they did not show any physical instability in accelerated stability studies, which also suggests long-term stability. Finally, the NCs antioxidant activity was evaluated. Interestingly, the NDGA and kaempferol mixture provided an antioxidant synergic effect. The NLC formulations’ cytotoxicity was tested in vitro in immortalized human keratinocytes (HaCaT). In addition, putative antioxidant effects of the developed NLC formulations against tert-butyl hydroperoxide (t-BHP)-induced oxidative stress were studied, and the NDGA-loaded NLC was revealed to be the one with the most protective effect. Therefore, we concluded that the naringenin, NDGA, and kaempferol incorporation into NLCs constitutes a promising strategy to increase their bioavailability and delivery to the skin.

scholarly journals The Antioxidant Effects of a Polyphenol-Rich Grape Pomace ExtractIn VitroDo Not CorrespondIn VivoUsing Exercise as an Oxidant Stimulus

2012 ◽  
Vol 2012 ◽  
pp. 1-14 ◽  
Author(s):  
Aristidis S. Veskoukis ◽  
Antonios Kyparos ◽  
Michalis G. Nikolaidis ◽  
Dimitrios Stagos ◽  
Nektarios Aligiannis ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Hydroxyl Radicals ◽  
Antioxidant Properties ◽  
Redox Status ◽  
Grape Pomace ◽  
Antioxidant Effects ◽  
Essential Food

Fruits, such as grapes, are essential food of the Mediterranean diet. Grape extracts have potent antioxidant and chemopreventive propertiesin vitro. Numerous studies have examined the effects of plant extract administration on redox status at rest in animals and humans but their results are controversial. However, there are no studies comparing thein vitroandin vivoeffects of plant extracts on oxidative stress using exercise as an oxidant stimulus. Thus, the aim of this study was to investigate whether a polyphenol-rich grape pomace extract of theVitis viniferaspecies possessesin vitroantioxidant properties and to examine whether these properties apply in anin vivomodel at rest and during exercise. Our findings indicate that the tested extract exhibits potentin vitroantioxidant properties because it scavenges the DPPH•and ABTS•+radicals and inhibits DNA damage induced by peroxyl and hydroxyl radicals. Administration of the extract in rats generally induced oxidative stress at rest and after exercise whereas exercise performance was not affected. Our findings suggest that the grape pomace extract does not behave with the same wayin vitroandin vivo.

scholarly journals Antioxidant Effects of Turmeric Leaf Extract against Hydrogen Peroxide-Induced Oxidative Stress In Vitro in Vero Cells and In Vivo in Zebrafish

Antioxidants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 112
Author(s):  
Sera Kim ◽  
Mingyeong Kim ◽  
Min-Cheol Kang ◽  
Hyun Hee L. Lee ◽  
Chi Heung Cho ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Properties ◽  
Vero Cells ◽  
G1 Phase ◽  
Ros Generation ◽  
Scavenging Activity ◽  
Intracellular Ros ◽  
Antioxidant Effects

Oxidative stress, caused by the excessive production of reactive oxygen species (ROS), results in cellular damage. Therefore, functional materials with antioxidant properties are necessary to maintain redox balance. Turmeric leaves (Curcuma longa L. leaves; TL) are known to have antioxidant properties, including 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 2,2′-Azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS), and Hydrogen peroxide (H2O2) radical scavenging activity in several studies. The antioxidant effects of TL come from distinct bioactive compounds, such as curcumin, total phenolic compounds, and flavonoids. Therefore, in this study, the antioxidant effects of a water extract of TL (TLE) against H2O2 treatment were assessed in vitro Vero cells and in vivo zebrafish models. The intracellular ROS generation and the proportion of sub-G1 phase cells were evaluated in H2O2- or/and TLE-treated Vero cells to measure the antioxidant activity of TLE. TLE showed outstanding intracellular ROS scavenging activity and significantly decreased the proportion of cells in the sub-G1 phase in a dose-dependent manner. Furthermore, cell death, ROS generation, and lipid peroxidation in the H2O2-treated zebrafish model were attenuated as a consequence of TLE treatment. Collectively, the results from this study suggested that TLE may be an alternative material to relieve ROS generation through its antioxidant properties or a suitable material for the application in a functional food industry.

scholarly journals Ethanolic Extract of Glycyrrhiza glabra to Ameliorate Oxidative Stress – Studies In vitro

2020 ◽  
Vol 10 (2) ◽  
pp. 2289-2297
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Glycyrrhiza Glabra ◽  
New Drugs ◽  
Phytochemical Analysis ◽  
Radical Scavenging Assay

Plants are major sources of bioactive organic molecules of interest for the pharmaceutical industry and are being screened for new drugs and chemicals. Glycyrrhiza glabra Linn. (Family: Fabaceae) is a very well-known medicinal plant traditionally used as medicine. Its roots and rhizomes are the medicinal parts used and are reported to possess antitumor, antimicrobial, antiviral, anti-inflammatory, immunoregulatory activities. The ethanolic extract of Glycyrrhiza glabra was prepared in the present work, and its phytochemical analysis and HPLC were done. The in vitro antioxidant activity assays such as DPPH radical scavenging assay, Hydroxyl radical scavenging assay, total antioxidant activity assay, and total reducing power assay was done, and the results showed significant antioxidant activity. The extract was analyzed further to evaluate the ability to protect against oxidative stress in chicken liver tissue. The extent of lipid peroxidation and glutathione in H2O2 and/or Glycyrrhiza glabra extract-treated tissue indicated the extract's potential to protect against oxidative stress under in vitro conditions. The in vitro comet assay results showed that the Glycyrrhiza glabra extract protected against H2O2 induced cellular DNA damage. These findings indicated promising antioxidant and antigenotoxic potential of Glycyrrhiza glabra and need further exploration for translating these findings to its possible health benefits.

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