The Effect of Physical Conditions on the Transportation of Peripheral Blood Progenitor (PBPC) Products.

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 2315-2315
Author(s):  
Jan Jansen ◽  
Pamela L Nolan ◽  
Margaret I Reeves ◽  
Luke Paul Akard ◽  
James M. Thompson ◽  
...  
Keyword(s):  
Trypan Blue ◽  
Room Temperature ◽  
Trypan Blue Exclusion ◽  
Physical Conditions ◽  
Cd34 Cells ◽  
Wide Range ◽  
Duration Of Storage ◽  
Higher Temperature ◽  
Storage Times ◽  
And Storage

Abstract The viability of transported PBPC products has not been studied extensively. Commonly, PBPC products are transported at a concentration of >200 x109/l in containers with −20oC ice packs. Continuous temperature monitoring has shown that the temperatures of these products stays at <10oC for less than 24 hours and reaches room temperature by 48 hours. Samples of freshly collected PBPC from 12 allogeneic donors were studied for various viability parameters during storage for up to 96 hours. The effects of storage time, concentration of cells, temperature, and storage in gas-permeable bags were studied. Trypan-blue exclusion and double fluorescence for 7-AAD and CD34 were used for viability assessment. Over a wide range of temperatures and storage times, the viable CD34+ assay was more sensitive to damage than trypan-blue exclusion (mean Δ 10.7%, p<0.0001 in paired t-test). The viable CD34+ assay was routinely used in parallel with CFU-GM cultures. No difference in survival of viable CD34+ cells or CFU-GM was found whether cells were incubated for 48hr in test-tubes or in gas-permeable bags. When cells at 200 x 109/l were incubated for 48hr at room temperature, the mean viability decreased to 19% and 6% of starting values of viable CD34+ cells and CFU-GM, respectively. Serial dilution to 25 x 109/l improved the survival to 81% and 51% respectively. Similarly, incubation at lower temperatures led to better survival of CD34+ cells and CFU-GM: 67% and 18% at 17oC, 80% and 50% at 13oC, and 95% and 86% at 4oC. At 200 x109/l and 22oC the survivals of CD34+ cells and CFU-GM were 74% and 21% at 24hr, 19% and 7% at 48hr, 7% and 6% at 72hr, and 3% and 13% at 96hr. The effects of concentration, temperature and duration of storage were all significant (p<0.05). Transportation at 4oC leads to the best survival of CD34+ cells and CFU-GM, in particular at a low concentration. If transportation at a slightly higher temperature is necessary, dilution of the PBPC product will enhance the survival of CD34+ cells and CFU-GM. Proliferative assays such as CFU-GM appear the most sensitive parameters of PBPC survival, and should be included in the validation process of PBPC transportation.

scholarly journals Key properties of inorganic thermoelectric materials – tables (version 1)

2022 ◽  
Author(s):  
Robert Freer ◽  
Dursun Ekren ◽  
Tanmoy Ghosh ◽  
Kanishka Biswas ◽  
Pengfei Qiu ◽  
...  
Keyword(s):  
Thermoelectric Properties ◽  
Conversion Efficiency ◽  
Thermoelectric Materials ◽  
Room Temperature ◽  
Temperature Data ◽  
Inorganic Materials ◽  
Peak Performance ◽  
Thermoelectric Conversion ◽  
Wide Range ◽  
Higher Temperature

Abstract This paper presents tables of key thermoelectric properties, which define thermoelectric conversion efficiency, for a wide range of inorganic materials. The 12 families of materials included in these tables are primarily selected on the basis of well established, internationally-recognised performance and their promise for current and future applications: Tellurides, Skutterudites, Half Heuslers, Zintls, Mg-Sb Antimonides, Clathrates, FeGa3–type materials, Actinides and Lanthanides, Oxides, Sulfides, Selenides, Silicides, Borides and Carbides. As thermoelectric properties vary with temperature, data are presented at room temperature to enable ready comparison, and also at a higher temperature appropriate to peak performance. An individual table of data and commentary are provided for each family of materials plus source references for all the data.

Effect of Temperature Fluctuation and Transport Time on Complete Blood Count Parameters

2019 ◽  
Vol 152 (Supplement_1) ◽  
pp. S112-S112
Author(s):  
Amandeep Kaur ◽  
Hong-Kee Lee ◽  
Denisse Del Giudice ◽  
Irene Singh ◽  
Mohamed Eldibany
Keyword(s):  
Temperature Fluctuation ◽  
Room Temperature ◽  
Optimal Transport ◽  
Complete Blood Count ◽  
White Cell Count ◽  
Blood Parameters ◽  
Effect Of Temperature ◽  
Duration Of Storage ◽  
And Storage

Abstract Introduction Reliable specimen collection and transport are fundamental to obtain high-quality test results in the hematology laboratory. Recognizing the effect of fluctuations in transport and storage conditions, time, and temperatures on the integrity of specimens and quality of results may help define the optimal transport and storage parameters. We designed an IRB-approved quality improvement study to investigate the effects of temperature fluctuation and duration of storage on blood parameters. Methods Ten volunteers donated blood for the study. All samples were deidentified and volunteers were blinded to the results without any review of their charts. Eight EDTA vials were collected from each volunteer and were immediately stored in heating blocks/refrigerators/freezers at selected temperatures including 0°C, 2 to 8°C, 25°C, 30°C, 35°C, 40°C, 45°C and at room temperature (21°C). Blood parameters were then measured using sysmex XN series at 0, 8, 12, 24, and 48 hours and values were analyzed. Results We observed that total white cell count, Hb, MCH, and platelets were stable for 12 hours at 2 to 35°C. RBCs and MPV were stable for 12 hours at 2 to 30°C, whereas blood parameters including Hct, RDW, MCV, and MCHC were stable up until 12 hours at room temperature but not at higher temperatures. All parameters were stable at 2 to 8°C even up to 48 hours. Conclusion It is preferable to perform CBC on blood specimens as soon as they are received. However, our data suggest that most of the blood parameters are stable for at least 12 hours at room temperature. The integrity of specimens and consequently the quality of the results deteriorate above 35°C and when stored longer than 24 hours; 2 to 8°C is the optimal temperature range for specimen transport, especially if delays in analysis are anticipated.

Optimum Temperature for Maintaining the Viability of CD34+ Cells during Storage and Transport of Fresh Hemopoietic Progenitor Cells.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 5269-5269
Author(s):  
Vicki Antonenas ◽  
Frances Garvin ◽  
Melina Webb ◽  
Mary Sartor ◽  
Kenneth F. Bradstock ◽  
...  
Keyword(s):  
Stem Cells ◽  
Progenitor Cells ◽  
Trypan Blue ◽  
Room Temperature ◽  
Storage Time ◽  
Optimum Temperature ◽  
Cd34 Cells ◽  
The Mean ◽  
Colony Assays ◽  
Hemopoietic Progenitor

Abstract Background and Aim: The optimum storage and transport of freshly harvested hemopoetic progenitor cells (HPC) in the liquid state is not specified in the JACIE and FACT guidelines. Depending upon transplant centre, there is a range of reported ideal temperatures (1°C to 24°C) for HPC storage and transport but little data exists to justify the recommendations. Due to the limitations of Trypan Blue viability assays and CFU-GM colony assays, we used a no-lyse, CD34 assay (Sartor et al, Bone Marrow Transplantation 2005) to determine the optimum storage and transport temperature for maintaining viability of CD34+ stem cells in freshly harvested HPC. Method: Samples were aseptically removed from 46 fresh HPC harvests (34 PBSC & 12 BM) and stored at refrigerated temperature (2°– 8°C), room temperature (18°– 24°C) and 37°C, for up to 72 hours. Samples were analysed for viable CD34+ cells/ml at 0, 24, 48 and 72 hours. Results: The mean viable CD34+ yield prior to storage was 7.7x106/kg (range: 0.7 – 30.3). No viable CD34+ cells remained after storage at 37°C for 24 hours. The mean % loss of viable CD34+ cells at refrigerated and room temperatures can be summarized as follows: Conclusion: These results demonstrate that the optimum temperature to maintain the viability of CD34+ stem cells for up to 72 hours during storage and transport of freshly harvested HPC is 2°– 8°C. Mean % loss or gain of CD34+ cells on storage Time Refrigerated Temperature Room Temperature N Mean Range N Mean Range 24 hrs 39 −9.4 +15 to −42 23 −21.9 +17 to −59 48 hrs 31 −19.4 +7 to −69 20 −30.7 +3 to −68 72 hrs 29 −28.0 +4 to −53 18 −43.3 +11 to −70

Storage of Orthodontic Study Models in Hospital Units in the U.K.

1992 ◽  
Vol 19 (3) ◽  
pp. 227-232 ◽  
Author(s):  
Niall J. McGuinness ◽  
Christopher D. Stephens
Keyword(s):  
Active Treatment ◽  
Diagnosis And Treatment ◽  
Legal Requirements ◽  
Hospital Units ◽  
Final Study ◽  
Wide Range ◽  
Significant Difference ◽  
Dental Records ◽  
Storage Times ◽  
And Storage

Orthodontic study models form an essential part of the dental records of patients undergoing diagnosis and treatment. In order to ascertain the problems encountered by hospital orthodontic units in the utilization and storage of study models, a questionnaire was circulated in February 1991 to members of the Consultant Orthodontists Group. All respondents took pretreatment study models, while 9 per cent took their final study models at some time other than the end of active treatment; 85·5 per cent of respondents stored their study models in their units, but most were beginning to experience difficulties in this regard. There was a wide range for storage times, and only 10 per cent of employing authorities had a stated policy on the storage of study models. There was a highly significant difference (P&z.Ltc;0·001) between the time that models are stored at present, and the desired storage times. Most respondents appeared to be rather uncertain about the precise medico-legal requirements concerning model storage. The implications for audit and medico-legal matters are discussed in the light of these findings.

scholarly journals Stability of lactoferrin and lysozyme in human milk at various temperatures and duration of storage

2021 ◽  
Vol 27 (2) ◽  
Author(s):  
◽  
Sumirah Budi Pertami ◽  
Agus Setyo Utomo ◽  
Siti Nur Arifah ◽  
◽  
...  
Keyword(s):  
Human Milk ◽  
Room Temperature ◽  
Storage Time ◽  
Enzyme Linked Immunosorbent Assay ◽  
Milk Samples ◽  
Duration Of Storage ◽  
Different Temperatures ◽  
Bioactive Content ◽  
The Stability ◽  
And Storage

Introduction: Exclusive breastfeeding, especially in the first six months, is essential for infants as it provides nutrition and protection against various diseases. Colostrum, which is found in the first breast milk produced, contains various protective factors, such as lactoferrin and lysozyme. Human milk can be stored at room temperature, refrigerated, or kept frozen. Several factors affect the stability of the bioactive content in human milk, such as temperature and storage time. The aim of this study was to measure the stability of lactoferrin and lysozyme levels in human milk during the first six hours (h) at different temperatures and compare it with that of frozen human milk. Methods: Human milk samples were obtained from 11 breastfeeding mothers using certain criteria. The human milk was stored at room temperature and 4°C for 1, 3, and 6 h and classified as never frozen, while frozen human milk was stored at -20°C for 1, 3, and 6 days. Measurement of the lactoferrin and lysozyme levels was performed using enzyme-linked immunosorbent assay. Results: The results showed that storage at room temperature significantly reduced lactoferrin and lysozyme levels. Lactoferrin levels in frozen human milk did not significantly decrease during the first six days. Meanwhile, the lysozyme levels in frozen human milk decreased significantly. Conclusion: The levels of lactoferrin and lysozyme in frozen human milk stored for the first six days were more stable than those stored at room temperature and 4°C in the first 6 h.

Viability of CD34+ Cells in Cryopreserved Cord Blood

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 4138-4138
Author(s):  
Young-woo Eom ◽  
Seong Hyun Jeong ◽  
Jin-Hyuk Choi ◽  
Seok Yun Kang ◽  
Hyun Woo Lee ◽  
...  
Keyword(s):  
Cord Blood ◽  
Esterase Activity ◽  
Death Rate ◽  
Trypan Blue ◽  
Caspase 3 ◽  
Annexin V ◽  
Trypan Blue Exclusion ◽  
Cell Death Rate ◽  
Cd34 Cells ◽  
Dna Contents

Abstract Purpose: On performing umbilical cord blood (UCB) transplantation, faster engraftment may lead better clinical outcome. Because transplanted viable cell count in UCB is related to the engraftment, we examined cryopreserved UCB cells with several methods after thawing. Methods: Viability of cryopreserved cells were examined with trypan blue, DNA contents analysis, caspase-3 activation test, intracellular esterase activity and Annexin-V/PI staining. Results: A total of 60 samples were used in this study. After thawing, 89% of the total MNCs and 84% of CD34+ cells were viable as identified by trypan blue exclusion assay. In the CD34+ cell population, the cell death rate was found to be 47 % by Annexin-V/PI staining and less than 5 % by DNA contents analysis. Caspase-3 activity failed to document apoptosis. The intracellular esterase activity test also showed a cell death rate of about 10–20 % at 2, 4, and 6 hours after thawing. Conclusion: Viable cells in UCB should be measured by several compensatory techniques rather than a single method. Discordance among Annexin-V/PI staining versus trypan blue exclusion, DNA contents analysis, and the caspase-3 activation test or intracellular esterase activity should be clarified in order to apply these techniques for actual cord blood transplantation.

Cost and Utilization Of Stored Autologous PBSC To Support Tandem ASCT In MM Patients In The Era Of Novel Agent Therapy

Blood ◽  
2013 ◽  
Vol 122 (21) ◽  
pp. 4507-4507
Author(s):  
Colin Phipps ◽  
Michael L. Linenberger ◽  
Damian J. Green ◽  
M. Corinna Palanca-Wessels ◽  
Pamela S. Becker ◽  
...  
Keyword(s):  
Cellular Therapy ◽  
Conflicts Of Interest ◽  
Novel Agents ◽  
Cell Dose ◽  
Cd34 Cells ◽  
Duration Of Storage ◽  
And Storage ◽  
Over Time ◽  
Novel Agent

Introduction Historically, autologous stem cell transplantation (ASCT) for multiple myeloma (MM) improves overall survival (OS) compared to conventional chemotherapy alone. Before the introduction of novel agent therapy, tandem ASCT, defined as a second ASCT within 180 days of the first, was an important option for suboptimal responses after an initial ASCT and thus collecting adequate peripheral blood stem cells (PBSCs) for 2 transplants has been considered standard of care. However, the role of tandem transplants is being challenged by novel agent maintenance strategies. Considering this changing landscape of MM therapy, we sought to evaluate the current PBSC collection and storage practices that set the CD34+ cell dose goal to be sufficient for 2 transplants. Methods We obtained clinical, PBSC collection and storage data on MM patients who underwent ASCT from 1993 to 2011 from the Autologous Transplant and Cellular Therapy Laboratory databases at the Fred Hutchinson Cancer Research Center. We determined frequencies and trends of all second ASCTs, including tandem, costs involved in PBSC collection, storage, and utilization of the product that remained cryopreserved for a second ASCT. Cell dose target at our center is 5 x 106 CD34+ cells/kg/transplant. To analyze trends over time, we divided the sample into groups of 3 or 4 year periods. Collection and cryopreservation costs for second ASCT were calculated by first determining the number of days required to collect sufficient PBSC for one ASCT, then the number of additional days to complete the actual collection, and the cumulative costs of long-term storage in our facility. Cost was estimated per July 2012 charges: 1 day PBSC collection $3,016, 1 day processing for cryopreservation $5,955, 1 year storage fees $408 ($34/month). The cost of mobilization chemotherapy and growth factors were not included. Results From May 1993 to June 2011, 889 MM patients underwent PBSC collection and ASCT (111 of 1000 excluded due to incomplete records). Median total PBSC collection days was 2 (range 1 – 10) with median yield of 13.18 x 106/kg CD34+ cells. Median days to collect sufficient cells for one ASCT was 1 day (1 – 9) and 383 patients collected adequate cells for 2 ASCTs after 1 apheresis procedure. Of 889 patients, 135 underwent a second ASCT within a median 14 months (2.5 – 113) of the first. Number of second ASCTs per time period: 1993 to 1995 – 9 of 39 MM patients undergoing ASCT (23%), 1996 to 1999 – 18 of 100 (18%), 2000 to 2003 – 15 of 162 (9%), 2004 to 2007 – 62 of 251 (25%), 2008 to 2011 – 31 of 337 (9%). Fifty patients underwent tandem ASCTs and these accounted for 89%, 72%, 7%, 24%, and 42% of all second ASCTs during the respective periods; the other 85 occurred > 6 months after the first. Number of additional days and associated costs to collect and store PBSC for a second ASCT: 5 days ($44,855), n=1 patient; 4 days ($35,844), n=2; 3 days ($26,913), n=10; 2 days ($17,942), n=41; and 1 day ($8,971), n=211. 637 patients had unused PBSC that remained in storage for ≥ 1 year, with a rising trend over time: 1993 to 1995 – 7 (1%), 1996 to 1999 – 65 (10%), 2000 to 2003 – 77 (12%), 2004 to 2007 – 185 (29%), 2008 to 2011 – 303 (48%). Duration of storage was < 2 years for 34, 2 to 5 years for 346, and > 5 years for 257 patients. Median PBSC storage time was 40 months. PBSC products from 260 patients were discarded (or sent to repository), after a median storage of 54 months, for the following reasons: 5 had allogeneic transplant, 74 were alive and possibly concerned about costs and/or the unlikely need for a second ASCT, 3 had cell viability issues, and 178 patients had died. From January 2009, some patients received plerixafor to achieve the collection goal for 1 ASCT. Its cost was not considered additional. Estimated average cost for PBSC collection, cryopreservation and storage was at least $20,065.96/person and at least $6718.11/person was spent to collect and store PBSC for a tandem ASCT that was never performed. Conclusions Approximately 70% of patients had PBSC products that remained unused and in prolonged cryopreservation after the first ASCT. Estimated cost for collection and storage of PBSC beyond that needed for a single ASCT accounted for roughly 1/3 of total costs. This conventional practice should be reconsidered in view of rising treatment costs, the evolving role of novel agents in maintenance therapy, and the deeper responses achievable with novel agents prior to first ASCT, thus reducing the need for tandem ASCT. Disclosures: No relevant conflicts of interest to declare.

scholarly journals Low-Temperature Growth of Shewanella oneidensis MR-1

2005 ◽  
Vol 71 (2) ◽  
pp. 811-816 ◽  
Author(s):  
Randa Abboud ◽  
Radu Popa ◽  
Virginia Souza-Egipsy ◽  
Carol S. Giometti ◽  
Sandra Tollaksen ◽  
...  
Keyword(s):  
Room Temperature ◽  
Doubling Time ◽  
Shewanella Oneidensis ◽  
Maximum Growth ◽  
Lag Phase ◽  
Temperature Growth ◽  
Wide Range ◽  
Mesophilic Bacterium ◽  
Cellular Lipids ◽  
Higher Temperature

ABSTRACT Shewanella oneidensis MR-1 is a mesophilic bacterium with a maximum growth temperature of ≈35°C but the ability to grow over a wide range of temperatures, including temperatures near zero. At room temperature (≈22°C) MR-1 grows with a doubling time of about 40 min, but when moved from 22°C to 3°C, MR-1 cells display a very long lag phase of more than 100 h followed by very slow growth, with a doubling time of ≈67 h. In comparison to cells grown at 22°C, the cold-grown cells formed long, motile filaments, showed many spheroplast-like structures, produced an array of proteins not seen at higher temperature, and synthesized a different pattern of cellular lipids. Frequent pilus-like structures were observed during the transition from 3 to 22°C.

scholarly journals KAJIAN MUTU DAN DAYA SIMPAN DENDENG UDANG PUTIH (Penaeus merguensis) SELAMA PENGEMASAN DAN PENYIMPANAN SUHU RUANG

AGROINTEK ◽  
2021 ◽  
Vol 15 (2) ◽  
pp. 608-616
Author(s):  
Yulia Delviani ◽  
Susi Lestari ◽  
Shanti Dwita Lestari ◽  
Sherly Ridhowati
Keyword(s):  
Room Temperature ◽  
White Shrimp ◽  
Plate Count ◽  
Total Volatile ◽  
Paired Sample ◽  
Total Plate Count ◽  
Average Water ◽  
Storage Times ◽  
And Storage ◽  
Total Yeast

The white shrimp (Penaeus merguensis)is abundant in Indonesia which could be made the jerky product to increase its shelflife. This study was aimed to determine the quality and shelflife of vacuum and non- vacuum  packed white shrimp jerky stored at room temperature. And,  the packed of white shrimp jerky was stored at room temperature for 0, 3, 6 and 9 days. The data obtained were analyzed using paired sample t-test. The condition of packaging had affected the quality and storage times for this jerky. The average water content  value was 23,01% (dry basis,db) for all the treatments. For 9 days, the TVB-N (total volatile based- nitrogen) and TPC (total plate count) were 22,41% mgN/100g and 3,27 log CFU/g (vacuum packed), then 26,52 mgN/100g and 6.12 log CFU/g (non-vacuum packed), respectively. During storage,  total yeast and fungi, as well as  aw (activity water), have increased. The vacuum- packaged white shrimp jerky was the best option for storing this product, and it has a shelflife of up to 9.

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