Storage of Orthodontic Study Models in Hospital Units in the U.K.

Orthodontic study models form an essential part of the dental records of patients undergoing diagnosis and treatment. In order to ascertain the problems encountered by hospital orthodontic units in the utilization and storage of study models, a questionnaire was circulated in February 1991 to members of the Consultant Orthodontists Group. All respondents took pretreatment study models, while 9 per cent took their final study models at some time other than the end of active treatment; 85·5 per cent of respondents stored their study models in their units, but most were beginning to experience difficulties in this regard. There was a wide range for storage times, and only 10 per cent of employing authorities had a stated policy on the storage of study models. There was a highly significant difference (P&z.Ltc;0·001) between the time that models are stored at present, and the desired storage times. Most respondents appeared to be rather uncertain about the precise medico-legal requirements concerning model storage. The implications for audit and medico-legal matters are discussed in the light of these findings.

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The Optimum Storage Conditions on the Quality of the Stingless bee Honey

Journal of Physics Conference Series ◽

10.1088/1742-6596/2049/1/012003 ◽

2021 ◽

Vol 2049 (1) ◽

pp. 012003

Author(s):

Imron Meechai ◽

Isma-ae Chelong ◽

Romlee Chedoloh

Keyword(s):

Moisture Content ◽

Physicochemical Properties ◽

Sugar Content ◽

Storage Condition ◽

Storage Conditions ◽

Stingless Bee ◽

Significant Difference ◽

Storage Times ◽

And Storage

Abstract Honey of stingless bee has a higher moisture content than bees. Long-term storage may cause fermentation processes to change the physicochemical properties and taste. Thus, the aim of this research was evaluation of the optimum storage condition on the quality of stingless bee honey. Stingless bee honey (Tetragonular larviceps) was contained in plastic bottle and kept at ambient temperature (30-35°C) and low temperature (4-8°C) for 0-45 days. Before and after storage honey were analyzed the physicochemical properties and sensory investigation for comparison of quality. The results showed that temperatures and storage times have affected on the reducing sugar content, pH, conductivity, color, moisture content with significant difference (p<0.05). While, temperatures and storage times have unaffected on the °Brix value (p>0.05). Additionally, the physicochemical properties of honey were according with previously quality report. The sensory investigation indicated that the smell natural flavor, consistency, taste and sourness were not significant difference (p>0.05). In contrast, the color and overall preference were significant difference (p<0.05). For honey quality, Thus, the honey might keep at 4-35°C for ≤45 day of this study.

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The Effect of Physical Conditions on the Transportation of Peripheral Blood Progenitor (PBPC) Products.

Blood ◽

10.1182/blood.v112.11.2315.2315 ◽

2008 ◽

Vol 112 (11) ◽

pp. 2315-2315

Author(s):

Jan Jansen ◽

Pamela L Nolan ◽

Margaret I Reeves ◽

Luke Paul Akard ◽

James M. Thompson ◽

...

Keyword(s):

Trypan Blue ◽

Room Temperature ◽

Trypan Blue Exclusion ◽

Physical Conditions ◽

Cd34 Cells ◽

Wide Range ◽

Duration Of Storage ◽

Higher Temperature ◽

Storage Times ◽

And Storage

Abstract The viability of transported PBPC products has not been studied extensively. Commonly, PBPC products are transported at a concentration of >200 x109/l in containers with −20oC ice packs. Continuous temperature monitoring has shown that the temperatures of these products stays at <10oC for less than 24 hours and reaches room temperature by 48 hours. Samples of freshly collected PBPC from 12 allogeneic donors were studied for various viability parameters during storage for up to 96 hours. The effects of storage time, concentration of cells, temperature, and storage in gas-permeable bags were studied. Trypan-blue exclusion and double fluorescence for 7-AAD and CD34 were used for viability assessment. Over a wide range of temperatures and storage times, the viable CD34+ assay was more sensitive to damage than trypan-blue exclusion (mean Δ 10.7%, p<0.0001 in paired t-test). The viable CD34+ assay was routinely used in parallel with CFU-GM cultures. No difference in survival of viable CD34+ cells or CFU-GM was found whether cells were incubated for 48hr in test-tubes or in gas-permeable bags. When cells at 200 x 109/l were incubated for 48hr at room temperature, the mean viability decreased to 19% and 6% of starting values of viable CD34+ cells and CFU-GM, respectively. Serial dilution to 25 x 109/l improved the survival to 81% and 51% respectively. Similarly, incubation at lower temperatures led to better survival of CD34+ cells and CFU-GM: 67% and 18% at 17oC, 80% and 50% at 13oC, and 95% and 86% at 4oC. At 200 x109/l and 22oC the survivals of CD34+ cells and CFU-GM were 74% and 21% at 24hr, 19% and 7% at 48hr, 7% and 6% at 72hr, and 3% and 13% at 96hr. The effects of concentration, temperature and duration of storage were all significant (p<0.05). Transportation at 4oC leads to the best survival of CD34+ cells and CFU-GM, in particular at a low concentration. If transportation at a slightly higher temperature is necessary, dilution of the PBPC product will enhance the survival of CD34+ cells and CFU-GM. Proliferative assays such as CFU-GM appear the most sensitive parameters of PBPC survival, and should be included in the validation process of PBPC transportation.

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Evaluation of the maladaptation of the test base in acrylic resin regarding the technique of preparation, place of measurement and storage time

Revista de Odontologia da UNESP ◽

10.1590/1807-2577.04517 ◽

2018 ◽

Vol 47 (1) ◽

pp. 51-56

Author(s):

Natália Pereira de OLIVEIRA ◽

Renata Pilli JÓIAS ◽

Rodnei Dennis ROSSONI ◽

Renato Morales JÓIAS

Keyword(s):

Storage Time ◽

Acrylic Resin ◽

Hydraulic Press ◽

Control Group ◽

Preparation Technique ◽

Test Base ◽

Significant Difference ◽

Storage Times ◽

And Storage ◽

Digital Caliper

Abstract Introduction The most used material for the preparation of the baseplates is the acrylic resin, but it can present distortions. Objective To evaluate preparation technique, region and storage time that presents less maladaptation of the base when made with self-cured acrylic resin. Material and method Models were created in gypsum type III simulating edentulous maxilla, as divided into 3 groups (n = 10): GC (control group) thermopolymerizable acrylic resin; G1 - manual adaptation technique and G2 - drip technique. For the measurements, silicone by condensation of light consistency that was interposed between base and model was used. With a hydraulic press, 50 kg pressure was applied leading the base of the model. The obtained mold was measured in the palate, canine and molar regions with a digital caliper at the following times: immediately after the base polymerization, at 24, 48, 72, 96 hours and one week. The results were submitted to statistical analysis. Result G1 presented maladaptation of 0.43 mm ± 0.10, while G2 obtained 0.39 mm ± 0.11. The lowest maladaptation occurred in the CG. The palate region presented greater maladaptation (0.52 ± 0.07) and the canine region, the lowest (CD = 0.27 mm ± 0.07 and CE = 0.27 ± 0.09); There was no statistically significant difference for storage times. Conclusion G2 presented lower values than G1, with no statistically significant difference. The palate region presented greater maladaptation, followed by molars and canines. The bases continued to maladaptation the model after the immediate polymerization, with no statistically significant difference.

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Efficiency of Different Diluents and Dilution Rates on the Fertilization Potential of Chicken Spermatozoa

Indian Journal of Animal Research ◽

10.18805/ijar.b-3940 ◽

2020 ◽

Author(s):

V. Beulah Pearlin ◽

J. Mohan ◽

J.S. Tyagi ◽

M. Gopi ◽

G. Kolluri ◽

...

Keyword(s):

Sperm Motility ◽

Fertile Period ◽

Semen Collection ◽

Wide Range ◽

Significant Difference ◽

Semen Storage ◽

Subsequent Storage ◽

And Storage ◽

Fertility Pattern ◽

Fresh Semen

Background: Artificial Insemination (AI) has been well established to obtain better fertility than natural mating. Semen extension and preservation play a major role in AI technique. The concentrated nature of chicken semen entails dilution of semen for easy handling and storage. With the availability of a wide range of extenders, exploring various extenders and dilution rates supplement the reproductive efficiency achieved through modern AI techniques.Methods: Pooled semen samples obtained from twenty White Leghorn breeder males were split equally into three portions, each for CARI poultry semen diluent, EK extender and Tselutin extender. At 1:2 dilution rate, the samples were subjected to sperm motility analysis from 0 to 96 h and AI was targeted at 0 and 24 h of semen collection. Fertility was assessed by candling of eggs collected from 2 to 8 days post insemination at 9th day of incubation. Further investigation of different dilution rates (1:2, 1:4, 1:6, 1:8, 1:10, 1:12, 1:14, 1:16, 1:18, 1:20) on fertility of fresh semen was done using the superior diluent.Result: Upon analysis, sperm motility showed no significant difference among the diluents at 0 h storage while CARI poultry semen diluent showed superior motility (%) at the subsequent storage periods, followed by EK and Tselutin extender. Higher fertility (p less than 0.05) was expressed in the diluents of CARI and EK at 0 h storage, whereas CARI poultry semen diluent showed superior fertility pattern (p less than 0.05) followed by EK and least by Tselutin extender at 24 h semen storage. Further investigation of different dilution rates using CARI diluent, exhibited fertility (p less than 0.05) of 90% and higher at 1:2 and 1:4 dilutions, followed by 1:6 and 1:8 at 2 to 6 days of fertile period.

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CLINICAL AND FORENSIC ASPECTS OF PHARMACOBEZOARS

Current Drug Research Reviews ◽

10.2174/2589977512666200217094018 ◽

2020 ◽

Vol 12 ◽

Author(s):

Francisco Basílio ◽

Ricardo Jorge Dinis-Oliveira

Keyword(s):

State Of The Art ◽

Signs And Symptoms ◽

Immediate Release ◽

Diagnosis And Treatment ◽

Blood Concentrations ◽

Forensic Practice ◽

Wide Range ◽

Therapeutic Doses ◽

Complete History ◽

Lethal Blood

Background: Pharmacobezoars are specific types of bezoars formed when medicines, such as tablets, suspensions, and/or drug delivery systems, aggregate and may cause death by occluding airways with tenacious material or by eluting drugs resulting in toxic or lethal blood concentrations. Objective: This work aims to fully review the state-of-the-art regarding pathophysiology, diagnosis, treatment and other relevant clinical and forensic features of pharmacobezoars. Results: patients of a wide range of ages and in both sexes present with signs and symptoms of intoxications or more commonly gastrointestinal obstructions. The exact mechanisms of pharmacobezoar formation are unknown but is likely multifactorial. The diagnosis and treatment depend on the gastrointestinal segment affected and should be personalized to the medication and the underlying factor. A good and complete history, physical examination, image tests, upper endoscopy and surgery through laparotomy of the lower tract are useful for diagnosis and treatment. Conclusion: Pharmacobezoars are rarely seen in clinical and forensic practice. They are related to controlled or immediate-release formulations, liquid or non-digestible substances, in normal or altered digestive motility/anatomy tract, and in overdoses or therapeutic doses, and should be suspected in the presence of risk factors or patients taking drugs which may form pharmacobezoars.

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Biochemical and molecular characterization of Alternaria alternata isolates highly resistant to procymidone from broccoli and cabbage

Phytopathology Research ◽

10.1186/s42483-021-00092-z ◽

2021 ◽

Vol 3 (1) ◽

Author(s):

Bingran Wang ◽

Tiancheng Lou ◽

Lingling Wei ◽

Wenchan Chen ◽

Longbing Huang ◽

...

Keyword(s):

Alternaria Alternata ◽

Single Point ◽

Sodium Dodecyl ◽

Cross Resistance ◽

Molecular Characteristics ◽

Single Point Mutation ◽

Wide Range ◽

Significant Difference ◽

Leaf Blights ◽

High Level

AbstractAlternaria alternata, a causal agent of leaf blights and spots on a wide range of hosts, has a high risk of developing resistance to fungicides. Procymidone, a dicarboximide fungicide (DCF), has been widely used in controlling Alternaria leaf blights in China for decades. However, the resistance of A. alternata against DCFs has rarely been reported from crucifer plants. A total of 198 A. alternata isolates were collected from commercial fields of broccoli and cabbage during 2018–2019, and their sensitivities to procymidone were determined. Biochemical and molecular characteristics were subsequently compared between the high-level procymidone-resistant (ProHR) and procymidone-sensitive (ProS) isolates, and also between ProHR isolates from broccoli and cabbage. Compared with ProS isolates, the mycelial growth rate, sporulation capacity and virulence of most ProHR isolates were reduced; ProHR isolates displayed an increased sensitivity to osmotic stresses and a reduced sensitivity to sodium dodecyl sulfate (SDS); all ProHR isolates showed a reduced sensitivity to hydrogen peroxide (H2O2) except for the isolate B102. Correlation analysis revealed a positive cross-resistance between procymidone and iprodione, or fludioxonil. When treated with 10 μg/mL of procymidone, both mycelial intracellular glycerol accumulations (MIGAs) and relative expression of AaHK1 in ProS isolates were higher than those in ProHR isolates. Sequence alignment of AaHK1 from ten ProHR isolates demonstrated that five of them possessed a single-point mutation (P94A, V612L, E708K or Q924STOP), and four isolates had an insertion or a deletion in their coding regions. No significant difference in biochemical characteristics was observed among ProHR isolates from two different hosts, though mutations in AaHK1 of the cabbage-originated ProHR isolates were distinct from those of the broccoli-originated ProHR isolates.

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Prostatic Artery Embolization (PAE) and Transurethral Resection of the Prostate (TURP) have a Differential Impact on Lower Urinary Tract Symptoms (LUTS): Retrospective Analysis of the Multicentre UK-ROPE (UK Register of Prostate Embolization) Study

CardioVascular and Interventional Radiology ◽

10.1007/s00270-021-02821-5 ◽

2021 ◽

Author(s):

Ganesh Vigneswaran ◽

Drew Maclean ◽

Mohammed Hadi ◽

Benjamin Maher ◽

Sachin Modi ◽

...

Keyword(s):

Retrospective Analysis ◽

Transurethral Resection ◽

Artery Embolization ◽

Prostatic Artery Embolization ◽

Voiding Symptoms ◽

Significant Difference ◽

Student’S T ◽

The Uk ◽

And Storage ◽

Prostatic Artery

Abstract Purpose To compare the relative IPSS (International Prostate Symptom Score) improvement in storage and voiding symptoms between prostatic artery embolization (PAE) and transurethral resection of the prostate (TURP). Method Retrospective analysis of the UK-ROPE (UK Register of Prostate Embolization) multicentre database was conducted with inclusion of all patients with full IPSS questionnaire score data. The voiding and storage subscore improvement was compared between interventions. Student’s t-test (paired and unpaired) and ANOVA (Analysis of variance) were used to identify significant differences between the groups. Results 146 patients (121 PAE, 25 TURP) were included in the analysis. Storage symptoms were more frequently the most severe symptom (‘storage’ in 75 patients vs ‘voiding’ in 17 patients). Between groups, no significant difference was seen in raw storage subscore improvement (TURP 4.9 vs PAE 4.2; p = 0.34) or voiding subscore improvement (TURP 8.4 vs PAE 6.7; p = 0.1). ANOVA demonstrated a greater proportionate reduction (relative to total IPSS) towards voiding symptoms in the TURP group (27.3% TURP vs 9.9% PAE, p = 0.001). Conclusion Although both TURP and PAE improve voiding symptoms more than storage, a significantly larger proportion of total symptom reduction is due to voiding in the TURP cohort, with PAE providing a more balanced improvement between voiding and storage.

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Antifungal Activity of Propyl Disulfide from Neem (Azadirachta indica) in Vapor and Agar Diffusion Assays against Anthracnose Pathogens (Colletotrichum gloeosporioides and Colletotrichum acutatum) in Mango Fruit

Microorganisms ◽

10.3390/microorganisms9040839 ◽

2021 ◽

Vol 9 (4) ◽

pp. 839

Author(s):

Muhammad Rafiullah Khan ◽

Vanee Chonhenchob ◽

Chongxing Huang ◽

Panitee Suwanamornlert

Keyword(s):

Antifungal Activity ◽

Mycelial Growth ◽

Colletotrichum Gloeosporioides ◽

Colletotrichum Acutatum ◽

Model Parameters ◽

Pathogenicity Test ◽

Agar Diffusion ◽

Wide Range ◽

Significant Difference ◽

Diffusion Assay

Microorganisms causing anthracnose diseases have a medium to a high level of resistance to the existing fungicides. This study aimed to investigate neem plant extract (propyl disulfide, PD) as an alternative to the current fungicides against mango’s anthracnose. Microorganisms were isolated from decayed mango and identified as Colletotrichum gloeosporioides and Colletotrichum acutatum. Next, a pathogenicity test was conducted and after fulfilling Koch’s postulates, fungi were reisolated from these symptomatic fruits and we thus obtained pure cultures. Then, different concentrations of PD were used against these fungi in vapor and agar diffusion assays. Ethanol and distilled water were served as control treatments. PD significantly (p ≤ 0.05) inhibited more of the mycelial growth of these fungi than both controls. The antifungal activity of PD increased with increasing concentrations. The vapor diffusion assay was more effective in inhibiting the mycelial growth of these fungi than the agar diffusion assay. A good fit (R2, 0.950) of the experimental data in the Gompertz growth model and a significant difference in the model parameters, i.e., lag phase (λ), stationary phase (A) and mycelial growth rate, further showed the antifungal efficacy of PD. Therefore, PD could be the best antimicrobial compound against a wide range of microorganisms.

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A Threat of Farmers’ Suicide and the Opportunity in Organic Farming for Sustainable Agricultural Development in India

Sustainability ◽

10.3390/su11082400 ◽

2019 ◽

Vol 11 (8) ◽

pp. 2400 ◽

Cited By ~ 4

Author(s):

Karthikeyan Mariappan ◽

Deyi Zhou

Keyword(s):

Organic Farming ◽

Tamil Nadu ◽

Agricultural Development ◽

Small Scale ◽

Conventional Farming ◽

Wide Range ◽

Significant Difference ◽

Small Scale Farmers ◽

The Cost ◽

Input Cost

Agriculture is the main sources of income for humans. Likewise, agriculture is the backbone of the Indian economy. In India, Tamil Nadu regional state has a wide range of possibilities to produce all varieties of organic products due to its diverse agro-climatic condition. This research aimed to identify the economics and efficiency of organic farming, and the possibilities to reduce farmers’ suicides in the Tamil Nadu region through the organic agriculture concept. The emphasis was on farmers, producers, researchers, and marketers entering the sustainable economy through organic farming by reducing input cost and high profit in cultivation. A survey was conducted to gather data. One way analysis of variance (ANOVA) has been used to test the hypothesis regards the cost and profit of rice production. The results showed that there was a significant difference in profitability between organic and conventional farming methods. It is very transparent that organic farming is the leading concept of sustainable agricultural development with better organic manures that can improve soil fertility, better yield, less input cost and better return than conventional farming. The study suggests that by reducing the cost of cultivation and get a marginal return through organic farming method to poor and small scale farmers will reduce socio-economic problems such as farmers’ suicides in the future of Indian agriculture.

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Recent Insights into Anthocyanin Pigmentation, Synthesis, Trafficking, and Regulatory Mechanisms in Rice (Oryza sativa L.) Caryopsis

Biomolecules ◽

10.3390/biom11030394 ◽

2021 ◽

Vol 11 (3) ◽

pp. 394 ◽

Cited By ~ 1

Author(s):

Enerand Mackon ◽

Guibeline Charlie Jeazet Dongho Epse Mackon ◽

Yafei Ma ◽

Muhammad Haneef Kashif ◽

Niyaz Ali ◽

...

Keyword(s):

Food Industry ◽

Anthocyanin Biosynthesis ◽

Oryza Sativa L ◽

Anthocyanin Synthesis ◽

Pigmented Rice ◽

Complex Processes ◽

Natural Colorants ◽

Wide Range ◽

Anthocyanin Production ◽

And Storage

Anthocyanins are antioxidants used as natural colorants and are beneficial to human health. Anthocyanins contribute to reactive oxygen species detoxification and sustain plant growth and development under different environmental stresses. They are phenolic compounds that are broadly distributed in nature and are responsible for a wide range of attractive coloration in many plant organs. Anthocyanins are found in various parts of plants such as flowers, leaves, stems, shoots, and grains. Considering their nutritional and health attributes, anthocyanin-enriched rice or pigmented rice cultivars are a possible alternative to reduce malnutrition around the globe. Anthocyanin biosynthesis and storage in rice are complex processes in which several structural and regulatory genes are involved. In recent years, significant progress has been achieved in the molecular and genetic mechanism of anthocyanins, and their synthesis is of great interest to researchers and the scientific community. However, limited studies have reported anthocyanin synthesis, transportation, and environmental conditions that can hinder anthocyanin production in rice. Rice is a staple food around the globe, and further research on anthocyanin in rice warrants more attention. In this review, metabolic and pre-biotic activities, the underlying transportation, and storage mechanisms of anthocyanins in rice are discussed in detail. This review provides potential information for the food industry and clues for rice breeding and genetic engineering of rice.

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