scholarly journals One-Step Quantitative Molecular Approach for Detection of BCR/ABL1 Rearrangement and for Monitoring of Minimal Residual Disease in CML Patients: An Inter Laboratory Study

Blood ◽  
2016 ◽  
Vol 128 (22) ◽  
pp. 5423-5423
Author(s):  
Filomena Daraio ◽  
Maria Teresa Bochicchio ◽  
Barbara Izzo ◽  
Claudia Venturi ◽  
Santa Errichiello ◽  
...  
Keyword(s):  
Reference Material ◽  
Clinical Samples ◽  
Assigned Value ◽  
Direct Testing ◽  
Individual Laboratory ◽  
European Reference Material ◽  
One Step ◽  
The Mean ◽  
The One ◽  
The Individual

Abstract Molecular tests are the best way to monitor CML course in patients under treatment with tyrosine kinase inhibitors (TKI). International guidelines indicate the absolute copy number of the control gene ABL1 as reference for the definition of the sensitivity of the analytical method. A general implementation of the International Guidelines (EUTOS) and the moving forward of current technologies towards one-step reactions, that allow direct testing from the patient RNA, require continuous verification of the method performances. Here, as Italian laboratory network for the standardization of CML diagnosis (LabNet), we performed a comparative study across the three reference laboratories in order to evaluate the inclusion of "BCR-ABL P210 ELITe MGB® Kit" (ELITechGroup S.p.A.) one-step assay among the technologies indicated in the Laboratory Recommendations and Indications (R.I.L.) of the Italian Network for CML monitoring. "BCR-ABL P210 ELITe MGB® Kit" is a new assay that allows to perform in a unique reaction the retro-transcription and the amplification of the extracted RNA sample. In this study 30 RNA extracted from whole blood samples of CML patients at different stages of the disease and centrally distributed to the other reference labs have been analyzed. All laboratories tested 300 ng per reaction of each RNA according the one-step approach and the same RNA according each own routine method. Moreover, in the same experiments, the European Reference Material certified plasmid ERM-AD623 has been evaluated. Our results show an increased analytical sensitivity in detection of both genes (BCR/ABL1 and ABL1): the limit of detection of the one-step reaction is as low as 0.001% IS BCR/ABL1. By testing the ERM-AD623 at 1 copy/reaction the rate of PCR positivities is 63%, and the average estimated quantity is 2.5 (SD = ± 1.5) copies/reaction. The linear measurement range of BCR/ABL1 and of the control gene ABL1 evaluated using the ERM-AD623 reference material are linear and equivalent in the range of 102-107 copies/reaction. Quantifications obtained with this kit are aligned to the European Reference Material. Using 7500 Fast Dx Real-Time PCR Instrument or 7900 Real-Time PCR System (Applied Biosystem, Thermo Fisher Scientific), we confirm that the calibrator of the "BCR-ABL P210 ELITe MGB® Kit" is aligned to the ERM-AD623 DNA international standard and we demonstrated the inter-laboratory low variability and good linearity of the method by processing the secondary reference material aligned to WHO primary reference material. By analysis of 30 RNA of CML patients we observed high results reproducibility among laboratories (figure 1). In addition, at comparison with the individual routine methods (ipsogen BCR-ABL1 Mbcr IS-MMR DX, P210 PHILADELPHIA Q-PCR Alert kit. and an home-made assay) we report up to 97.4% correlation of BCR-ABL P210 ELITe MGB® kit results. In conclusion, our data demonstrate that "BCR-ABL P210 ELITe MGB® Kit" is a rapid, reproducible assay, aligned and calibrated towards the current goal standards BCR/ABL1 assays. It allows direct testing from RNA samples while maintaining the desired sensitivity. By requiring reduced hands on time of the operators and by allowing direct testing of RNA, "BCR-ABL P210 ELITe MGB® kit" will provide a significant improvement in the standardization of the molecular approach to CML monitoring. Figure 1 BCR-ABL P210 ELITe MGB® Kit reproducibility with clinical samples. Data of the individual laboratory were plotted against the mean assigned value. The regression fit of all data is R-Sq=96.8%. Figure 1. BCR-ABL P210 ELITe MGB® Kit reproducibility with clinical samples. Data of the individual laboratory were plotted against the mean assigned value. The regression fit of all data is R-Sq=96.8%. Disclosures Castagnetti: Bristol-Myers Squibb: Consultancy, Honoraria; Novartis: Consultancy, Honoraria; Pfizer: Consultancy, Honoraria; ARIAD Pharmaceuticals: Consultancy, Honoraria. Martinelli:ARIAD: Consultancy; Genentech: Consultancy; Roche: Consultancy; Amgen: Consultancy; MSD: Consultancy; Pfizer: Consultancy, Speakers Bureau; BMS: Speakers Bureau; Genentech: Consultancy; Amgen: Consultancy; Novartis: Speakers Bureau; MSD: Consultancy; Roche: Consultancy; ARIAD: Consultancy; Pfizer: Consultancy, Speakers Bureau. Saglio:Novartis: Consultancy, Honoraria; BMS: Consultancy, Honoraria; ARIAD: Consultancy, Honoraria; Pfizer: Consultancy, Honoraria; Roche: Consultancy, Honoraria.

scholarly journals Rapid electrochemical detection of coronavirus SARS-CoV-2

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Thanyarat Chaibun ◽  
Jiratchaya Puenpa ◽  
Tatchanun Ngamdee ◽  
Nimaradee Boonapatcharoen ◽  
Pornpat Athamanolap ◽  
...  
Keyword(s):  
Electrochemical Biosensor ◽  
Rolling Circle Amplification ◽  
Clinical Samples ◽  
Rolling Circle ◽  
Qrt Pcr ◽  
Reverse Transcription Pcr ◽  
Sandwich Hybridization ◽  
Redox Active ◽  
One Step ◽  
The One

AbstractCoronavirus disease 2019 (COVID-19) is a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Diagnosis of COVID-19 depends on quantitative reverse transcription PCR (qRT-PCR), which is time-consuming and requires expensive instrumentation. Here, we report an ultrasensitive electrochemical biosensor based on isothermal rolling circle amplification (RCA) for rapid detection of SARS-CoV-2. The assay involves the hybridization of the RCA amplicons with probes that were functionalized with redox active labels that are detectable by an electrochemical biosensor. The one-step sandwich hybridization assay could detect as low as 1 copy/μL of N and S genes, in less than 2 h. Sensor evaluation with 106 clinical samples, including 41 SARS-CoV-2 positive and 9 samples positive for other respiratory viruses, gave a 100% concordance result with qRT-PCR, with complete correlation between the biosensor current signals and quantitation cycle (Cq) values. In summary, this biosensor could be used as an on-site, real-time diagnostic test for COVID-19.

On the capture of interstellar matter by stars

1940 ◽  
Vol 36 (3) ◽  
pp. 314-322 ◽  
Author(s):  
R. d'E. Atkinson
Keyword(s):  
Radial Velocity ◽  
The Other ◽  
Interstellar Matter ◽  
Centre Of Gravity ◽  
Compelling Reason ◽  
Other Hand ◽  
The Mean ◽  
The One ◽  
The Individual ◽  
Capture Radius

The derivation given by Hoyle and Lyttleton for an accretion formula proposed by them is examined. A number of arguments against its validity are put forward, especially that on the one hand their capture radius depends on the theorem that if the velocity of certain masses of gas after collision is less than the velocity of escape at the point, they will not in fact escape, while on the other hand it is clear (and is now admitted) that the gas cannot in fact move with this velocity at all. It is also shown that since, ex hypothesi, the individual molecules will all, on the average, retain their hyperbolic velocities, there is not the compelling reason for their capture that there appeared to be in Hoyle and Lyttleton's argument, where only the mean radial velocity of the centre of gravity of the mass was considered. Further, it seems improbable that the temperature of the interstellar matter can be low enough for the initial assumptions of their theory to hold.

Simultaneous analysis of fluorescence decay curves for the one-step determination of the mean aggregation number of aqueous micelles

1988 ◽  
Vol 146 (3-4) ◽  
pp. 337-342 ◽  
Author(s):  
Noël Boens ◽  
Hongwen Luo ◽  
Mark van der Auweraer ◽  
Steven Reekmans ◽  
Frans C. De Schryver ◽  
...  
Keyword(s):  
Aggregation Number ◽  
Fluorescence Decay ◽  
Simultaneous Analysis ◽  
One Step ◽  
The Mean ◽  
The One ◽  
Aqueous Micelles

scholarly journals Smart Recommendations for Renting Bikes in Bike-Sharing Systems

2021 ◽  
Vol 11 (20) ◽  
pp. 9654
Author(s):  
Holger Billhardt ◽  
Alberto Fernández ◽  
Sascha Ossowski
Keyword(s):  
Queuing Theory ◽  
Real Data ◽  
Transport Systems ◽  
Bike Sharing ◽  
Usage Patterns ◽  
One Step ◽  
The One ◽  
The Individual ◽  
Recommendation Strategy ◽  
Better Than

Vehicle-sharing systems—such as bike-, car-, or motorcycle-sharing systems—have become increasingly popular in big cities in recent years. On the one hand, they provide a cheaper and environmentally friendlier means of transportation than private cars, and on the other hand, they satisfy the individual mobility demands of citizens better than traditional public transport systems. One of their advantages in this regard is their availability, e.g., the possibility of taking (or leaving) a vehicle almost anywhere in a city. This availability obviously depends on different strategic and operational management decisions and policies, such as the dimension of the fleet or the (re)distribution of vehicles. Agglutination problems—where, due to usage patterns, available vehicles are concentrated in certain areas, whereas no vehicles are available in others—are quite common in such systems, and need to be dealt with. Research has been dedicated to this problem, specifying different techniques to reduce imbalanced situations. In this paper, we present and compare strategies for recommending stations to users who wish to rent or return bikes in station-based bike-sharing systems. Our first contribution is a novel recommendation strategy based on queuing theory that recommends stations based on their utility to the user in terms of lower distance and higher probability of finding a bike or slot. Then, we go one step further, defining a strategy that recommends stations by combining the utility of a particular user with the utility of the global system, measured in terms of the improvement in the distribution of bikes and slots with respect to the expected future demand, with the aim of implicitly avoiding or alleviating balancing problems. We present several experiments to evaluate our proposal with real data from the bike sharing system BiciMAD in Madrid.

Cycle day of ovulation

1972 ◽  
Vol 4 (4) ◽  
pp. 371-378 ◽  
Author(s):  
William H. James
Keyword(s):  
Cycle Length ◽  
Composite Sample ◽  
Normal Length ◽  
Menstrual Cycles ◽  
The Mean ◽  
Long Cycles ◽  
The One ◽  
The Individual ◽  
Mean Cycle

The length of the post-ovulatory phase in a composite sample of menstrual cycles increases linearly with cycle length until cycle length reaches about 30 days, but thereafter it ceases to increase (Vollman, 1953; Bailey & Marshall, 1970). By contrast, plotted against the mean cycle length of the individual, the length of the post-ovulatory phase continues to increase roughly linearly with mean cycle length. It is concluded (a) that many long cycles are aberrant occurrences among cycles of normal length and are due to lengthening of the pre-ovulatory phase and (b) that where the mean cycle length is long, ovulation, as in shorter cycles, occurs just before the middle of the cycle, so that the post-ovulatory phase is longer than usual.The most fertile day seems to be the one before mid-cycle. These conclusions are the basis for the following formula for calendar rhythm:If x is the shortest and y the longest cycle in a woman's menstrual record, the ‘unsafe’ days start on Day (½x − 5) and last for (y − x + 8) days.

Cohort fertility of Polish women, 1945–2015: the context of postponement and recuperation

2018 ◽  
pp. 5-23 ◽  
Author(s):  
Krzysztof Tymicki ◽  
Krystof Zeman ◽  
Dorota Holzer-Żelażewska
Keyword(s):  
Driving Forces ◽  
Cohort Analysis ◽  
Individual Data ◽  
Cohort Fertility ◽  
The Mean ◽  
The One ◽  
The Individual ◽  
The Postponement ◽  
Second Births

The article is a follow-up and an extension to previously published papers by Holzer-Żelażewska & Holzer (1997) and Holzer-Żelażewska & Tymicki (2009). Fristly, we have added new cohorts to the cohort analysis based on the individual data from births registration for the years 2009–2015. Secondly, we have extended the scope of the study by taking into account the context of postponement and recuperation to analyses of cohort fertility of Polish women. The approach applied to the fertility postponement and recuperation on the cohort data refers to the method which was originally proposed by Frejka (2011) and Lesthaeghe (2001) and further developed by Sobotka et al. (Sobotka et al., 2011). This method allows for calculation of fertility postponement and recuperation measures with respect to a benchmark cohort chosen as the one that first experiences an onset of the increase in the mean age of motherhood at first birth. The results show the remarkable changes in the fertility patterns in Poland. The main driving forces behind the change in fertility patterns in Poland are related to the postponement of first births along with a relatively good recuperation. The magnitude of recuperation for Polish cohorts dropped significantly for second births and was almost non-existent for third and higher births. Therefore, the pattern of fertility in Poland observed till 2015 could be characterized by postponement and recuperation of first births along with a significant decrease in second births with perpetual postponement of third and higher births.

Hapten-heterologous conjugates evaluated for application to free thyroxine immunoassays

1993 ◽  
Vol 39 (2) ◽  
pp. 256-262 ◽  
Author(s):  
M J Khosravi ◽  
A Papanastasiou-Diamandi
Keyword(s):  
Solid Phase ◽  
Free Thyroxine ◽  
Clinical Samples ◽  
Time Resolved ◽  
Standard Curve ◽  
Sequential Binding ◽  
One Step ◽  
Titration Step ◽  
The One ◽  
Derivatives Of

Abstract We evaluated the effect of hapten heterology on free thyroxine (FT4) immunoassays involving the biotin-streptavidin system and time-resolved fluorometry. We compared protein derivatives of thyroxine (T4) and triiodothyronine (T3) as solid-phase antigen or biotinylated protein-tracer conjugate for competitive (or sequential) binding to a mouse anti-T4 monoclonal antibody. In both one- and two-step assays, the heterologous combination of the antibody and T3 conjugates showed superior standard curve sensitivity but up to eightfold lower zero standard signal (B(o)) when the same amounts of antibody and conjugates were used. The improved sensitivity was not altered when the amount of coupled T3 was increased to obtain a B(o) value similar to that of the homologous combination of antibody and T4 conjugates. In the two-step format, the sensitivity of the homologous assay was insufficient for routine use, consistent with displacement of bound T4 during the antibody back-titration step (demonstrated in the T4 displacement experiment with excess conjugate). Results from the one-step (labeled antibody) heterologous assay for approximately 85 clinical samples correlated well with those from an immunofluorometric assay and a two-step radioimmunoassay. The assay was not affected by a wide variation in endogenous serum concentrations of T4-binding globulin and albumin.

scholarly journals Bonding Performance of a Hydrophilic Amide Monomer Containing Adhesive to Occlusal and Cervical Dentin

Materials ◽  
2020 ◽  
Vol 13 (21) ◽  
pp. 4727
Author(s):  
Eri Seitoku ◽  
Shuhei Hoshika ◽  
Takatsumi Ikeda ◽  
Shigeaki Abe ◽  
Toru Tanaka ◽  
...  
Keyword(s):  
Bond Strength ◽  
Water Storage ◽  
Adhesive System ◽  
Adhesive Failure ◽  
Adhesive Systems ◽  
Microtensile Bond Strength ◽  
Bonding Performance ◽  
One Step ◽  
The Mean ◽  
The One

This study aimed to evaluate the bonding performance of a new one-step self-etching adhesive system containing a novel hydrophilic amide monomer. Clearfil Universal Bond Quick (CUB) and Clearfil Megabond 2 (CMB) were used as the one-step and two-step adhesive systems, respectively. Flat dentin surfaces of human premolars were exposed using #600 SiC (silicon carbide) and bonded with the respective adhesives of each system. The teeth were sectioned to obtain beams (1 mm × 1 mm) after 24 h of water storage. The mean bond strength and standard deviations (MPa) on an occlusal surface were as follows: CUB: 45.9 ± 19.7 and CMB: 67.9 ± 25.3. The values for cervical ones were CUB: 56.0 ± 20.3 and CMB: 67.6 ± 16.0, respectively. In both conditions, the microtensile bond strength (μTBS) value was lower than that of CMB. As seen during the microscopic observation, no adhesive failure was observed after μTBS testing because CUB formed a firm and tight adhesive interface.

scholarly journals Rapid Electrochemical Detection of Coronavirus SARS-CoV-2.

2020 ◽  
Author(s):  
Thanyarat Chaibun ◽  
Jiratchaya Puenpa ◽  
Tatchanun Ngamdee ◽  
Nimaradee Boonapatcharoen ◽  
Pornpat Athamanolap ◽  
...  
Keyword(s):  
Electrochemical Biosensor ◽  
Rolling Circle Amplification ◽  
Clinical Samples ◽  
Rolling Circle ◽  
Qrt Pcr ◽  
Reverse Transcription Pcr ◽  
Sandwich Hybridization ◽  
Redox Active ◽  
One Step ◽  
The One

Abstract COVID-19 is a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Diagnosis of COVID-19 depends on quantitative reverse transcription PCR (qRT-PCR), which is time-consuming and requires expensive instrumentation. Here, we report an ultrasensitive electrochemical biosensor based on isothermal rolling circle amplification (RCA) for rapid detection of SARS-CoV-2. The assay involves the hybridization of the RCA amplicons with probes that were functionalized with redox active labels that are detectable by an electrochemical biosensor. The one-step sandwich hybridization assay could detect as low as 1 copy/mL of N and S genes, in less than 2 hours. Sensor evaluation with 105 clinical samples, including 40 SARS-CoV-2 positive and 9 samples positive for other respiratory viruses, gave a 100% concordance result with qRT-PCR, with complete correlation between the biosensor current signals and quantitation cycle (Cq) values. In summary, this biosensor could be used as an on-site, real-time diagnostic test for COVID-19.

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