scholarly journals Studies on Platelet Proteins. IV. Some Physical and Chemical Properties of Thrombosthenin

Blood ◽  
1969 ◽  
Vol 34 (4) ◽  
pp. 511-520 ◽  
Author(s):  
P. GANGULY
Keyword(s):  
Protein A ◽  
Gel Filtration ◽  
Chemical Properties ◽  
Sedimentation Coefficient ◽  
Viscosity Data ◽  
Kinetic Unit ◽  
Platelet Protein ◽  
Low Protein ◽  
Approximate Molecular Weight ◽  
Physical And Chemical

Abstract The contractile platelet protein thrombosthenin has been isolated with butanol and purified further by repeated precipitation and gel filtration. Thrombosthenin thus isolated shows all the typical properties of this protein as reported in the literature. 1 M NaCl-tris, pH 7.2 has been found to be a better solvent than 0.6 M KCl as far as stability, resolution and aggregation of the protein are concerned. In the ultracentrifuge, 0.7 per cent thrombosthenir in 0.6 M KCl shows three hypersharp boundaries with sedimentation coefficients of 36 S, 56 S and 83 S. However, at low protein concentration only the 36 S boundary is observed. In 1 M NaCl, all these components break down producing a single kinetic unit of 18 S which dissociates further to 7 S. The 7 S component retains the ATP-sensitivity typical of thrombosthenin, suggesting it to be the monomeric unit of this protein. A similar hypersharp boundary with similar sedimentation coefficient to purified thrombosthenin has been noted in platelet ghosts solubilized in presence of detergent. A combination of sedimentation and viscosity data leads to an approximate molecular weight 8.9 x 105 for thrombosthenin.

RAPESEED: RELATIONS BETWEEN SOME PHYSICAL AND CHEMICAL PROPERTIES

1976 ◽  
Vol 56 (1) ◽  
pp. 169-174 ◽  
Author(s):  
RUSSELL TKACHUK ◽  
F. D. KUZINA
Keyword(s):  
Oil Content ◽  
Chemical Properties ◽  
Kernel Weight ◽  
Erucic Acid Content ◽  
Brassica Napus L ◽  
Weight Density ◽  
Low Protein ◽  
Undesirable Property ◽  
Almost All ◽  
Physical And Chemical

Rapeseed (Brassica napus L., Brassica campestris L.) was examined for its density, hectoliter weight, porosity, kernel weight, and protein, oil, moisture, and erucic acid content, and iodine value. Inter-relationship and correlations were established between these properties. An increase of from 4 to 10% in moisture content of rapeseed resulted in only a slight decrease in both density and hectoliter weight. Density increased significantly with increase in hectoliter weight. However, in almost all samples, kernel weight was inversely related to density and hence to hectoliter weight. Heavier kernels contained more oil, while the opposite was shown for denser kernels. An inverse relationship was found between hectoliter weight and oil content, showing that low hectoliter weight, usually considered an undesirable property, can actually indicate high oil content. However, low hectoliter weight appears to be associated with low protein content.

scholarly journals Physico-Chemical Properties of Purified Carboxylesterase from the Seeds of Tamarindus indica

2021 ◽  
pp. 42-58
Author(s):  
S. Kantharaju ◽  
M. Mylarappa
Keyword(s):  
Molecular Weight ◽  
Gel Filtration ◽  
Chemical Properties ◽  
Physical And Chemical Properties ◽  
Tamarindus Indica ◽  
Physico Chemical ◽  
Sds Page ◽  
Physical And Chemical ◽  
Naphthyl Acetate ◽  
And Chemical Properties

The present work is focus on physical and chemical properties of purified Carboxylesterase using the Seeds of Tamarindus Indica.The esterases are extracted from the germinating tamarind seeds using 50 mM phosphate buffer, pH 7 and purified. The Km with α-naphthyl acetate, β-naphthyl acetate and α-naphthyl butyrate as the substrates is 28.6 μM, 22.2 μM and 26.7 μM respectively. The Vmax for the same substrates is 7.1 x 10-3 µmole/min, 7.41 x 10-3 µmole/min and 8.00 x 10-3 µmole/min respectively. The enzymes optimally active at pH 7.0 and are stable between pH 5.0 to 8.0. The optimum temperature of esterase activity is 40˚C. The molecular weight of 27.5 kD as determined by SDS-PAGE, both in the presence and absence of β-mercaptothanol and is in close agreement with the molecular weight determined by gel-filtration on Sephadex G-100 (26.9 kD).

scholarly journals Some physical and chemical properties of trypsin-digested nucleoprotein

1968 ◽  
Vol 109 (4) ◽  
pp. 625-635 ◽  
Author(s):  
Dawn B. Marks ◽  
Verne N. Schumaker
Keyword(s):  
Intrinsic Viscosity ◽  
Chemical Properties ◽  
Sedimentation Coefficient ◽  
Physical And Chemical Properties ◽  
Peptide Complex ◽  
Dna Molecule ◽  
Calf Thymus ◽  
Hydrodynamic Data ◽  
Physical And Chemical ◽  
And Chemical Properties

A DNA–peptide complex that is soluble in 0·2m-sodium chloride can be prepared by trypsin digestion of calf thymus nucleoprotein. The trypsin-digested nucleoprotein molecule contains about 70% of DNA and 30% of peptides by weight, and consists of one DNA molecule associated with arginine-rich peptides. A series of trypsin-digested nucleoprotein preparations differing only in molecular weight were prepared by blending. The intrinsic viscosity and average sedimentation coefficient were determined for each of these preparations. Then the DNA was isolated from each preparation and the hydrodynamic measurements were repeated on the DNA. From a comparison of these results it was concluded that the presence of the complex-forming peptides causes a large decrease in intrinsic viscosity of the DNA and an increase in sedimentation coefficient. In addition, the hydrodynamic data indicate that the DNA–peptide complex behaves like a coil in solution but is more compact than the same length of DNA. The ‘melting’ profiles, streptomycin precipitation curves and maximum viscosities obtained with ethidium bromide binding for the trypsin-digested nucleoprotein are similar to those of purified DNA, and markedly different from those of undigested nucleoprotein. These findings suggest that the peptides are not strongly associated with the DNA, and that secondary valency forces are involved in the binding.

Statistical criteria of stellar population types

1966 ◽  
Vol 24 ◽  
pp. 101-110
Author(s):  
W. Iwanowska
Keyword(s):  
Stellar Population ◽  
Chemical Properties ◽  
Spectrophotometric Study ◽  
Physical And Chemical Properties ◽  
Population Type ◽  
The Galaxy ◽  
Distribution Parameters ◽  
Physical And Chemical ◽  
Statistical Criteria ◽  
And Chemical Properties

In connection with the spectrophotometric study of population-type characteristics of various kinds of stars, a statistical analysis of kinematical and distribution parameters of the same stars is performed at the Toruń Observatory. This has a twofold purpose: first, to provide a practical guide in selecting stars for observing programmes, second, to contribute to the understanding of relations existing between the physical and chemical properties of stars and their kinematics and distribution in the Galaxy.

Biophysical Measurement of Molecular Weight of Foot-and-Mouth Disease RNA Fragments

1974 ◽  
Vol 32 ◽  
pp. 262-263
Author(s):  
Sydney S. Breese ◽  
Howard L. Bachrach
Keyword(s):  
Chemical Properties ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Distilled Water ◽  
Bovine Plasma ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Carbon Coated ◽  
Rna Fragments ◽  
Physical And Chemical

Continuing studies on the physical and chemical properties of foot-and-mouth disease virus (FMDV) have included electron microscopy of RNA strands released when highly purified virus (1) was dialyzed against demlneralized distilled water. The RNA strands were dried on formvar-carbon coated electron microscope screens pretreated with 0.1% bovine plasma albumin in distilled water. At this low salt concentration the RNA strands were extended and were stained with 1% phosphotungstic acid. Random dispersions of strands were recorded on electron micrographs, enlarged to 30,000 or 40,000 X and the lengths measured with a map-measuring wheel. Figure 1 is a typical micrograph and Fig. 2 shows the distributions of strand lengths for the three major types of FMDV (A119 of 6/9/72; C3-Rezende of 1/5/73; and O1-Brugge of 8/24/73.

Tailoring microstructures of materials through biomimetics

1993 ◽  
Vol 51 ◽  
pp. 500-501
Author(s):  
Mehmet Sarikaya ◽  
Ilhan A. Aksay
Keyword(s):  
Chemical Properties ◽  
Design And Synthesis ◽  
Structure Sensitive ◽  
Macro Scale ◽  
Methods Of Synthesis ◽  
Successive Level ◽  
Engineering Materials ◽  
Physical And Chemical ◽  
And Chemical Properties ◽  
Synthesis Of Materials

Biomimetics involves investigation of structure, function, and methods of synthesis of biological composite materials. The goal is to apply this information to the design and synthesis of materials for engineering applications.Properties of engineering materials are structure sensitive through the whole spectrum of dimensions from nanometer to macro scale. The goal in designing and processing of technological materials, therefore, is to control microstructural evolution at each of these dimensions so as to achieve predictable physical and chemical properties. Control at each successive level of dimension, however, is a major challenge as is the retention of integrity between successive levels. Engineering materials are rarely fabricated to achieve more than a few of the desired properties and the synthesis techniques usually involve high temperature or low pressure conditions that are energy inefficient and environmentally damaging.In contrast to human-made materials, organisms synthesize composites whose intricate structures are more controlled at each scale and hierarchical order.

Physico-Chemical Properties of Recombinant Desulphatohirudin

1990 ◽  
Vol 63 (03) ◽  
pp. 499-504 ◽  
Author(s):  
A Electricwala ◽  
L Irons ◽  
R Wait ◽  
R J G Carr ◽  
R J Ling ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Gel Filtration ◽  
Chemical Properties ◽  
Alpha Helix ◽  
Apparent Molecular Weight ◽  
Correlation Spectroscopy ◽  
Nmr Studies ◽  
Recombinant Hirudin ◽  
Physico Chemical ◽  
Recombinant Molecule

SummaryPhysico-chemical properties of recombinant desulphatohirudin expressed in yeast (CIBA GEIGY code No. CGP 39393) were reinvestigated. As previously reported for natural hirudin, the recombinant molecule exhibited abnormal behaviour by gel filtration with an apparent molecular weight greater than that based on the primary structure. However, molecular weight estimation by SDS gel electrophoresis, FAB-mass spectrometry and Photon Correlation Spectroscopy were in agreement with the theoretical molecular weight, with little suggestion of dimer or aggregate formation. Circular dichroism studies of the recombinant molecule show similar spectra at different pH values but are markedly different from that reported by Konno et al. (13) for a natural hirudin-variant. Our CD studies indicate the presence of about 60% beta sheet and the absence of alpha helix in the secondary structure of recombinant hirudin, in agreement with the conformation determined by NMR studies (17)

Response of corn (Zea mays L.) to various organic-based fertilizers in marginal upland of Sta. Rita, Samar

2017 ◽  
pp. 31-43
Author(s):  
Berta Ratilla ◽  
Loreme Cagande ◽  
Othello Capuno
Keyword(s):  
Block Design ◽  
Management Strategies ◽  
Chemical Properties ◽  
Organic Fertilizers ◽  
Growth And Yield ◽  
Physical And Chemical Properties ◽  
Total N ◽  
Randomized Complete Block Design ◽  
Physical And Chemical ◽  
And Chemical Properties

Organic farming is one of the management strategies that improve productivity of marginal uplands. The study aimed to: (1) evaluate effects of various organic-based fertilizers on the growth and yield of corn; (2) determine the appropriate combination for optimum yield; and (3) assess changes on the soil physical and chemical properties. Experiment was laid out in Randomized Complete Block Design, with 3 replications and 7 treatments, namely; T0=(0-0-0); T1=1t ha-1 Evans + 45-30-30kg N, P2O5, K2O ha-1; T2=t ha-1 Wellgrow + 45-30-30kg N, P2O5, K2O ha-1; T3=15t ha-1 chicken dung; T4=10t ha-1 chicken dung + 45-30-30kg N, P2O5, K2O ha-1; T5=15t ha-1 Vermicast; and T6=10t ha-1 Vermicast + 45-30-30kg N, P2O5, K2O ha-1. Application of organic-based fertilizers with or without inorganic fertilizers promoted growth of corn than the control. But due to high infestation of corn silk beetle(Monolepta bifasciata Horns), its grain yield was greatly affected. In the second cropping, except for Evans, any of these fertilizers applied alone or combined with 45-30-30kg N, P2O5, K2O ha-1 appeared appropriate in increasing corn earyield. Soil physical and chemical properties changed with addition of organic fertilizers. While bulk density decreased irrespective of treatments, pH, total N, available P and exchangeable K generally increased more with chicken dung application.

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