Basophil/mast cell precursors in human peripheral blood

Abstract Semisolid (methylcellulose) hemopoietic cultures revealed the presence of histamine-containing granulocyte colonies derived from precursors (CFU-C) present in human peripheral blood. Light microscopy and histochemical studies of cells in individual histamine-containing colonies demonstrated homogeneous populations of metachromatic basophil/mast cells (BMC) at various stages of maturation. By inverted microscopy, pure BMC colonies were more often found to have the overall appearance of the previously described “eosinophil” (type II), rather than “neutrophil-macrophage” (type I), colony type. Histamine-positive colonies constituted 58% (50/86) of all (type I and type II) granulocyte colonies in repeated cultures from a patient with systemic mastocytosis (SM), and 19% (13/67) of colonies in cultures from 8 patients with chronic myeloid leukemia (CML); this was in contrast to 8% (12/153) of colonies in cultures from 4 patients with urticaria pigmentosa (UP) and 6 normal controls (p less than 0.0001). Calculated frequency of BMC CFU-C was approximately 1 per 2 X 10(6) in normal and 1 per 2 X 10(5) nucleated cells in SM peripheral blood. Taking colony size into account, histamine content per cell in histamine-positive type II colonies in SM cultures was 1.1 +/- 0.19 pg, compared to 0.29 +/- 0.08 pg in CML and less than or equal to 0.10 in normals and UP. Electron microscopy (EM) of individual colonies revealed electron-dense granules with ultrastructural features of BMC in histamine-positive, but not histamine-negative, colonies. Use of these methods may help to further clarify the nature of BMC precursors and the regulation of their proliferation in bone marrow disorders and allergic states.

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Basophil/mast cell precursors in human peripheral blood

Blood ◽

10.1182/blood.v61.4.775.bloodjournal614775 ◽

1983 ◽

Vol 61 (4) ◽

pp. 775-780

Author(s):

JA Denburg ◽

M Richardson ◽

S Telizyn ◽

J Bienenstock

Keyword(s):

Peripheral Blood ◽

Myeloid Leukemia ◽

Systemic Mastocytosis ◽

Human Peripheral Blood ◽

Type I ◽

Type Ii ◽

Positive Type ◽

Dense Granules ◽

Normal Controls ◽

Bone Marrow Disorders

Semisolid (methylcellulose) hemopoietic cultures revealed the presence of histamine-containing granulocyte colonies derived from precursors (CFU-C) present in human peripheral blood. Light microscopy and histochemical studies of cells in individual histamine-containing colonies demonstrated homogeneous populations of metachromatic basophil/mast cells (BMC) at various stages of maturation. By inverted microscopy, pure BMC colonies were more often found to have the overall appearance of the previously described “eosinophil” (type II), rather than “neutrophil-macrophage” (type I), colony type. Histamine-positive colonies constituted 58% (50/86) of all (type I and type II) granulocyte colonies in repeated cultures from a patient with systemic mastocytosis (SM), and 19% (13/67) of colonies in cultures from 8 patients with chronic myeloid leukemia (CML); this was in contrast to 8% (12/153) of colonies in cultures from 4 patients with urticaria pigmentosa (UP) and 6 normal controls (p less than 0.0001). Calculated frequency of BMC CFU-C was approximately 1 per 2 X 10(6) in normal and 1 per 2 X 10(5) nucleated cells in SM peripheral blood. Taking colony size into account, histamine content per cell in histamine-positive type II colonies in SM cultures was 1.1 +/- 0.19 pg, compared to 0.29 +/- 0.08 pg in CML and less than or equal to 0.10 in normals and UP. Electron microscopy (EM) of individual colonies revealed electron-dense granules with ultrastructural features of BMC in histamine-positive, but not histamine-negative, colonies. Use of these methods may help to further clarify the nature of BMC precursors and the regulation of their proliferation in bone marrow disorders and allergic states.

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Expression of Gonadotropin-Releasing Hormone Type-I (GnRH-I) and Type-II (GnRH-II) in Human Peripheral Blood Mononuclear Cells (PMBCs) and Regulation of B-Lymphoblastoid Cell Proliferation by GnRH-I and GnRH-II

Experimental and Clinical Endocrinology & Diabetes ◽

10.1055/s-2004-830404 ◽

2004 ◽

Vol 112 (10) ◽

pp. 587-594 ◽

Cited By ~ 14

Author(s):

F. Tanriverdi ◽

D. Gonzalez-Martinez ◽

L. Silveira ◽

Y. Hu ◽

G. Maccoll ◽

...

Keyword(s):

Cell Proliferation ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Human Peripheral Blood ◽

Gonadotropin Releasing Hormone ◽

Type I ◽

Type Ii ◽

Peripheral Blood Mononuclear ◽

Blood Mononuclear Cells

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Structure of the two promoters of the human lck gene: differential accumulation of two classes of lck transcripts in T cells

Molecular and Cellular Biology ◽

10.1128/mcb.9.5.2173-2180.1989 ◽

1989 ◽

Vol 9 (5) ◽

pp. 2173-2180

Author(s):

T Takadera ◽

S Leung ◽

A Gernone ◽

Y Koga ◽

Y Takihara ◽

...

Keyword(s):

T Cells ◽

T Cell ◽

Cell Lines ◽

Carcinoma Cell ◽

Human Peripheral Blood ◽

Specific Gene ◽

Type I ◽

Type Ii ◽

Carcinoma Cell Lines ◽

Human T Cell

The human T-cell- or lymphocyte-specific gene, lck, encodes a tyrosine kinase and is a member of the src family. In this report we demonstrate that there are two classes of human lck transcripts (types I and II), containing different 5'-untranslated regions, which are expressed from two distinct promoters. No apparent sequence similarity was observed between the 5'-flanking regions of the two promoters. The expression of lck in human T-cell leukemia and carcinoma cell lines and in human peripheral blood T lymphocytes was examined by S1 nuclease and primer extension mapping and by Northern (RNA) blot analysis of total cellular RNA. The following results were obtained. (i) Two RNA start sites in the downstream promoter were used to generate type I transcripts. (ii) The major human type I start site has not been described for the mouse. (iii) At least five RNA start sites in the upstream promoter were used to generate type II transcripts. (iv) In T cells and in two colon carcinoma cell lines, type II transcripts were present in higher amounts than type I transcripts. (v) In T cells treated with phytohemagglutinin, tetradecanoylphorbol acetate, and cyclosporin A, the modulation of lck expression was associated primarily with changes in levels of type II transcripts. The above results suggest that the two human lck promoters are utilized differentially and may be regulated independently during certain physiological states.

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Effect of tunicamycin on maturation of fetal mouse lung

AJP Lung Cellular and Molecular Physiology ◽

10.1152/ajplung.1993.265.3.l250 ◽

1993 ◽

Vol 265 (3) ◽

pp. L250-L259

Author(s):

E. H. Webster ◽

S. R. Hilfer ◽

R. L. Searls ◽

J. Kornilow

Keyword(s):

Extracellular Matrix ◽

Fetal Lung ◽

Mouse Lung ◽

Type I ◽

Type Ii ◽

Fetal Mouse ◽

Type Ii Pneumocytes ◽

Separate Control ◽

Normal Controls

The mesodermal capsule of the fetal lung plays a role in differentiation of the respiratory region. It has been proposed for other epithelial organs that the mesodermal capsule influences development by modifying the basal lamina or the extended extracellular matrix. The effect could be on deposition or turnover of collagens, proteoglycans, and/or glycoproteins. This study tests the role of glycoproteins in differentiation of respiratory endings by inhibiting their synthesis with the antibiotic tunicamycin (TM). Lungs at 16 and 18 days gestation and 3 days after birth were cultured with TM and examined for morphological and biochemical differences from normal controls. With TM, alveolar regions did not expand properly and formed fewer type I pneumocytes, although type II pneumocytes were unaffected. The epithelium of untreated respiratory regions showed greater incorporation of radioactive mannose than the airways region or mesenchyme. This incorporation was diminished in TM, but the pattern persisted. Comparison with the results obtained with beta-xyloside suggested that differentiation of type I and type II pneumocytes is under separate control.

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Immunocytochemical localization of the endogenous neuroexcitotoxin quinolinate in human peripheral blood monocytes/macrophages and the effect of human T-cell lymphotropic virus type I infection.

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.93.4.1636 ◽

1996 ◽

Vol 93 (4) ◽

pp. 1636-1641 ◽

Cited By ~ 11

Author(s):

C. N. Venkateshan ◽

R. Narayanan ◽

M. G. Espey ◽

J. R. Moffett ◽

D. C. Gajdusek ◽

...

Keyword(s):

T Cell ◽

Virus Type ◽

Peripheral Blood ◽

Human Peripheral Blood ◽

Type I ◽

Immunocytochemical Localization ◽

Blood Monocytes ◽

Peripheral Blood Monocytes ◽

Human T Cell

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TLR7 Stimulation of APCs Results in Inhibition of IL-5 through Type I IFN and Notch Signaling Pathways in Human Peripheral Blood Mononuclear Cells

The Journal of Immunology ◽

10.4049/jimmunol.1200780 ◽

2013 ◽

Vol 190 (6) ◽

pp. 2585-2592 ◽

Cited By ~ 15

Author(s):

Susan Edwards ◽

Carolyn Jones ◽

Andrew J. Leishman ◽

Barbara W. Young ◽

Hiroyuki Matsui ◽

...

Keyword(s):

Notch Signaling ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Human Peripheral Blood ◽

Type I ◽

Peripheral Blood Mononuclear ◽

Blood Mononuclear Cells ◽

Tlr7 Stimulation ◽

Stimulation Of

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Type I and II Interferon Signatures Can Predict the Response to Anti-TNF Agents in Inflammatory Bowel Disease Patients: Involvement of the Microbiota

Inflammatory Bowel Diseases ◽

10.1093/ibd/izaa216 ◽

2020 ◽

Vol 26 (10) ◽

pp. 1543-1553

Author(s):

Clio P Mavragani ◽

Adrianos Nezos ◽

Nikolas Dovrolis ◽

Nikolaos-Panayiotis Andreou ◽

Evangelia Legaki ◽

...

Keyword(s):

Real Time ◽

Peripheral Blood ◽

Response To Treatment ◽

Type I ◽

Type Ii ◽

Lower Baseline ◽

Inflammatory Bowel ◽

Polymerase Chain ◽

Treatment Responses ◽

Necrosis Factor

Abstract Background Anti-TNF agents have been a cornerstone of IBD therapy; however, response to treatment has been variable, and clinically applicable biomarkers are urgently needed. We hypothesized that the type I and type II interferon (IFN) signatures may be a confounding factor for response to antitumor necrosis factor (TNF) treatment via interactions with the host and its gut microbiota. Methods Peripheral blood from 30 IBD patients and 10 healthy controls was subjected to real-time quantitative real-time polymerase chain reaction for type I and type II IFN genes (IFNGs), both at baseline and after treatment with anti-TNF. Correlation between IFN signatures and microbiota composition was also determined for a subgroup of patients and controls. Results At baseline, type I IFN score was significantly higher in IBD patients (P = 0.04 vs controls). Responders to subsequent anti-TNF treatment had significantly lower baseline scores for both type I and II IFN signatures (P < 0.005 vs nonresponders for both comparisons). During treatment with anti-TNF, the expression of type I and II IFNGs was significantly elevated in responders and decreased in nonresponders. In addition, changes in IFN signatures correlated to specific alterations in the abundance of several microbial taxa of the gut microbiome. Conclusions Baseline expression of type I and II IFN signatures and their kinetics during anti-TNF administration significantly correlate to treatment responses in IBD patients. Peripheral blood IFN signatures may serve as clinically meaningful biomarkers for the identification of subgroups of patients with favorable response to anti-TNF treatment. Additionally, the distinct synergies between different IFN types and microbiota might help drive therapeutic intervention.

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