scholarly journals Two-dimensional iodopeptide mapping demonstrates that erythrocyte Rh D, c, and E polypeptides are structurally homologous but nonidentical

Blood ◽  
1988 ◽  
Vol 72 (4) ◽  
pp. 1424-1427
Author(s):  
D Blanchard ◽  
C Bloy ◽  
P Hermand ◽  
JP Cartron ◽  
AM Saboori ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Molecular Species ◽  
Two Dimensional ◽  
Structural Homology ◽  
Composite Pattern ◽  
Rh Proteins ◽  
The Individual ◽  
High Degree

The 32,000 molecular weight (mol wt) erythrocyte Rh D, c, and E polypeptides were separately purified from cDE/cDE erythrocytes by monoclonal immunoprecipitations and compared by two-dimensional iodopeptide mapping. Digestions of the isolated Rh polypeptides with alpha-chymotrypsin revealed a high degree of structural homology between c and E (13/14 iodopeptides were identical) and less striking homology between D and c or E (8/19 identical). The iodopeptide maps of Rh proteins purified by a nonimmunologic protocol from cDE/cDE erythrocytes were virtually identical to the composite pattern (D + c + E) deduced from the individual maps of Rh D, c, and E iodopeptides. Digestions of the isolated Rh polypeptides with trypsin revealed an overall homology of approximately 50% between iodopeptides derived from D, c, and E. These data indicate that the erythrocyte Rh D, c, and E antigens are carried by homologous but distinct molecular species; c and E appear more closely related to each other than to D.

scholarly journals Two-dimensional iodopeptide mapping demonstrates that erythrocyte Rh D, c, and E polypeptides are structurally homologous but nonidentical

Blood ◽  
1988 ◽  
Vol 72 (4) ◽  
pp. 1424-1427 ◽  
Author(s):  
D Blanchard ◽  
C Bloy ◽  
P Hermand ◽  
JP Cartron ◽  
AM Saboori ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Molecular Species ◽  
Two Dimensional ◽  
Structural Homology ◽  
Composite Pattern ◽  
Rh Proteins ◽  
The Individual ◽  
High Degree

Abstract The 32,000 molecular weight (mol wt) erythrocyte Rh D, c, and E polypeptides were separately purified from cDE/cDE erythrocytes by monoclonal immunoprecipitations and compared by two-dimensional iodopeptide mapping. Digestions of the isolated Rh polypeptides with alpha-chymotrypsin revealed a high degree of structural homology between c and E (13/14 iodopeptides were identical) and less striking homology between D and c or E (8/19 identical). The iodopeptide maps of Rh proteins purified by a nonimmunologic protocol from cDE/cDE erythrocytes were virtually identical to the composite pattern (D + c + E) deduced from the individual maps of Rh D, c, and E iodopeptides. Digestions of the isolated Rh polypeptides with trypsin revealed an overall homology of approximately 50% between iodopeptides derived from D, c, and E. These data indicate that the erythrocyte Rh D, c, and E antigens are carried by homologous but distinct molecular species; c and E appear more closely related to each other than to D.

Heterologous radioimmunoassays for monkey gonadotrophins.

1983 ◽  
Vol 104 (1) ◽  
pp. 15-22 ◽  
Author(s):  
Shafiq A. Khan ◽  
Egon Diczfalusy
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Molecular Species ◽  
Adsorption Chromatography ◽  
Binding Studies ◽  
Linear Dose ◽  
Chloramine T ◽  
Partial Dissociation ◽  
High Degree ◽  
Dose Dependent

Abstract. The reliability of the reagents used in heterologous immunoassay procedures for FSH in monkey sera was assessed. Re-investigation of the iodination and purification of the human FSH tracer confirmed the presence of different iodinated molecular species, such as a high molecular weight aggregated material, 'intact' human FSH and subunits. Tracers obtained with the chloramine T procedure were somewhat superior to those obtained by the lactoperoxidase method. Purification by cellulose adsorption chromatography resulted in partial dissociation of the intact hormone into free subunits, while Sephadex G-25 chromatography did not yield any subunit-like material. Purification by Ultrogel AcA 54 gave the best separation of the 'intact' iodinated FSH. Binding studies indicated that an anti-ovine FSH (oFSH) serum showed a high degree of binding to 'intact' hFSH, although its binding to subunits and to aggregated material was also significant. The highest degree of binding to this antiserum was shown by the 'intact' tracer iodinated with chloramine T and purified sequentially by Sephadex G-25 and Ultrogel (AcA 54) chromatography. Using a highly purified hFSH tracer in combination with three antisera (anti-oFSH, H 31; anti-hFSH, WHO, M-93-2; anti-hFSH, NIH, batch No. 5) displacement curves were investigated with three purified monkey FSH preparations. No displacement was observed with a widely available anti-hFSH (WHO, M 93-2) serum. However, linear dose dependent displacement was found with another anti-hFSH (NIH, batch No. 5) serum and with an anti-oFSH (H 31) serum. Comparison of immunoassays with the human and ovine antiserum revealed significant differences in slope, parallelism, precision and effective range. Significant discrepancies in the FSH potency estimates in monkey sera were also observed with the two assay systems. It is suggested that – until a homologous system is developed – the immunoassay employing an hFSH tracer and an anti-oFSH serum should be used for macaque FSH.

Reconstruction of Two-Dimensional Projections of GP 32*I

1981 ◽  
Vol 39 ◽  
pp. 38-39
Author(s):  
H.A. Cohen ◽  
W. Chiu ◽  
J. Hosoda
Keyword(s):  
Electron Microscopy ◽  
Molecular Weight ◽  
Uranyl Acetate ◽  
Distilled Water ◽  
Acetate Solution ◽  
Two Dimensional ◽  
Parent Molecule ◽  
T4 Bacteriophage ◽  
Electron Imaging ◽  
Better Than

GP 32 (molecular weight 35000) is a T4 bacteriophage protein that destabilizes the DNA helix. The fragment GP32*I (77% of the total weight), which destabilizes helices better than does the parent molecule, crystallizes as platelets thin enough for electron diffraction and electron imaging. In this paper we discuss the structure of this protein as revealed in images reconstructed from stained and unstained crystals.Crystals were prepared as previously described. Crystals for electron microscopy were pelleted from the buffer suspension, washed in distilled water, and resuspended in 1% glucose. Two lambda droplets were placed on grids over freshly evaporated carbon, allowed to sit for five minutes, and then were drained. Stained crystals were prepared the same way, except that prior to draining the droplet, two lambda of aqueous 1% uranyl acetate solution were applied for 20 seconds. Micrographs were produced using less than 2 e/Å2 for unstained crystals or less than 8 e/Å2 for stained crystals.

Semiology of the Pain Syndrome - Identifying the Ideal Methods of Locoregional Anesthesia Based on Their Rationale and Features

2017 ◽  
Vol 68 (10) ◽  
pp. 2373-2377
Author(s):  
Mihaela Monica Scutariu ◽  
Vlad Danila ◽  
Corina Ciupilan ◽  
Oana Elena Ciurcanu
Keyword(s):  
Maxillofacial Surgery ◽  
Essential Element ◽  
Pain Syndrome ◽  
Oral And Maxillofacial Surgery ◽  
Structuring Element ◽  
Technical Accuracy ◽  
Locoregional Anesthesia ◽  
Special Approach ◽  
The Individual ◽  
High Degree

Anesthesia and the degree of control over the perception of pain depends on the personality of the individual, the socio-economic conditions, potential previous painful experiences and, last but not least, on fatigue and fear of the dentist. The perception of pain in patients is closely connected to their mental state. Pain is defined as a sensation of discomfort, with wide variations, both in quality and intensity, for different people in seemingly identical conditions; an unpleasant sensitive and emotional phenomena connected to the threat of a wound or caused in the tissues or described in the terms of this disease. The essential element of any type of anesthesia is analgesia, an effect which in some cases cannot be achived, due to the patient�s particularities or the physician�s lack of experience in anesthesia. Locoregional anesthesia (LRA) represents the blocking of the nociceptive sensitive and sympathetic autonomic afferents as well as that of motor efferents at the level of peripheral nerves� axons, by means of local anesthetic. To achieve the set purpose, we carried out a study on a representative human sample comprised of 10.123 patients treated in the Oral and Maxillofacial Surgery Clinic (Ambulatory) from the County Clinic Emergency Hospital St. Spiridon Iasi, between 01.01.2015-31.12.2016. The reason for the exclusion of certain categories of patients in the reseach was: the patients with a special conditions background require individual pre-anesthesia schemes, personalised for the nature of the pre-existing general condition, which must be further approved by the attending specialist physician : cardiologist, internist, diabetologist; children under 18 years old, with a high degree of anxiety; a high precentage of elderly patients, over 60 years old, possess a combination of general issues, thus requiring a special approach. The thoroughness lying at the core of the anesthetic practice, most especially the safegurading of a technical accuracy in the performance of anesthesia [12,], instead of improvisations, the lack of anatomical and stomatological training in general and the resulting inefficiency as such, is the underlying in-depth structuring element of this paper.

scholarly journals Territories of state-led aquaculture risk management: Thailand’s Plang Yai program

2020 ◽  
pp. 239965442096524
Author(s):  
Mariska JM Bottema ◽  
Simon R Bush ◽  
Peter Oosterveer
Keyword(s):  
Risk Management ◽  
Policy Instruments ◽  
Spatially Explicit ◽  
Financial Risks ◽  
Shared Resources ◽  
Globalizing World ◽  
Farmer Behavior ◽  
Key Policy ◽  
The Individual ◽  
High Degree

The Thai aquaculture sector faces a range of production, market and financial risks that extend beyond the private space of farms to include public spaces and shared resources. The Thai state has attempted to manage these shared risks through its Plang Yai (or ‘Big Area’) agricultural extension program. Using the lens of territorialization, this paper investigates how, through the Plang Yai program, risk management is institutionalized through spatially explicit forms of collaboration amongst farmers and between farmers and (non-)state actors. We focus on how four key policy instruments brought together under Plang Yai delimited multiple territories of risk management over shrimp and tilapia production in Chantaburi and Chonburi provinces. Our findings demonstrate how these policy instruments address risks through dissimilar but overlapping territories that are selectively biased toward facilitating the individual management of production risks, whilst enabling both the individual and collective management of market and financial risks. This raises questions about the suitability of addressing aquaculture risks by controlling farmer behavior through state-led designation of singular, spatially explicit areas. The findings also indicate the multiple roles of the state in territorializing risk management, providing a high degree of flexibility, which is especially valuable in landscapes shared by many users, connected to (global) value chains and facing diverse risks. In doing so we demonstrate that understanding the territorialization of production landscapes in a globalizing world requires a dynamic approach recognizing the multiplicity of territories that emerge in risk management processes.

A comparison of the compressibilities of some gases with that of nitrogen at pressures below one atmosphere

1950 ◽  
Vol 200 (1061) ◽  
pp. 201-218 ◽  
Keyword(s):  
Carbon Dioxide ◽  
Differential Method ◽  
Linear Extrapolation ◽  
Volume Changes ◽  
Pure Nitrogen ◽  
Molecular Weights ◽  
Nitrous Oxide Oxygen ◽  
The Individual ◽  
Straight Lines ◽  
High Degree

A differential method for comparing the compressibilities of gases at pressures below 1 atm. has been developed in which many of the errors inherent in methods employed previously have been to a large extent eliminated, especially those due to meniscus volume changes and capillary depression. Using pure nitrogen as a standard the low-pressure isothermals of carbon monoxide, carbon dioxide, nitrous oxide, oxygen, ethylene and propane have been determined at a temperature of 22-05° C. The deviations of the individual points from straight lines do not in most cases exceed 2 parts in 100,000. In no case, even with propane, was any curvature in the isotherms detectable. The contention of Moles and other recent workers that the molecular weights of liquefiable gases can be determined to a high degree of accuracy by linear extrapolation is rendered highly probable by this fresh evidence.

scholarly journals Composite patterns in neutral/neutral two-dimensional gels demonstrate inefficient replication origin usage.

1996 ◽  
Vol 16 (9) ◽  
pp. 4915-4922 ◽  
Author(s):  
R F Kalejta ◽  
J L Hamlin
Keyword(s):  
Replication Origin ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Yeast Cells ◽  
Eukaryotic Cells ◽  
Mapping Technique ◽  
Two Dimensional ◽  
Composite Pattern ◽  
Composite Gel ◽  
Bona Fide

The neutral/neutral two-dimensional (2-D) gel replicon mapping technique has been used to great advantage to localize and characterize origins of replication. Interestingly, many yeast origins display a composite pattern consisting of both a bubble arc and a single-fork arc. Moreover, in every instance in which neutral/neutral 2-D gels have been used to analyze origins in higher eukaryotic cells, two or more adjacent fragments display these composite patterns. We believe that composite patterns signal inefficient origin usage in yeast cells because the replicators in question are not active in every cell cycle and in higher eukaryotic replicons because initiation sites are chosen from among many potential sites lying within a zone. However, others have suggested that the single-fork arcs in these composite gel patterns arise from nicking activity that converts replication bubbles to branched structures that comigrate with bona fide single forks. Here, we have used three different replicon mapping strategies to show that broken simian virus 40 replication bubbles trace unique arcs that are clearly distinguishable from classic, intact single forks. Thus, it is likely that composite 2-D gel patterns represent origins that are inefficiently utilized.

Composition of and variation in high- and low-molecular weight glutenin subunits, and omega gliadins in Ethiopian tetraploid wheat germplasm

2006 ◽  
Vol 4 (2) ◽  
pp. 134-143 ◽  
Author(s):  
Faris Hailu ◽  
Eva Johansson ◽  
Arnulf Merker ◽  
Getachew Belay ◽  
Harjit-Singh ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Tetraploid Wheat ◽  
Glutenin Subunit ◽  
Low Molecular Weight ◽  
Glutenin Subunits ◽  
Hmw Glutenin Subunits ◽  
Lmw Glutenin Subunits ◽  
Hmw Glutenin ◽  
Omega Gliadins ◽  
High Degree

A collection of 120 Ethiopian tetraploid wheat accessions was analysed for high-molecular weight (HMW) glutenin subunit, low-molecular weight (LMW) glutenin subunit and omega gliadin composition by SDS–PAGE. For the HMW glutenin subunits, a new allelic variant, 2****, was detected which has not been previously described at the Glu-A1 locus. A high proportion of Glu-A1x banding pattern was observed in durum wheat. For the Glu-B1 locus four different banding patterns were detected. Among those HMW glutenin subunits, 7+8 were the most common, while subunits 14+15 and 6+8 were found to be rare. A high degree of variation was evident for the LMW glutenin subunits and D-zone omega gliadins. The association of the composition of the gluten with quality has been discussed. This wide variation can be used in improving the quality of wheat and to widen its genetic base.

scholarly journals Identification of two intermediates during processing of profilaggrin to filaggrin in neonatal mouse epidermis.

1984 ◽  
Vol 99 (4) ◽  
pp. 1372-1378 ◽  
Author(s):  
K A Resing ◽  
K A Walsh ◽  
B A Dale
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Tandem Repeats ◽  
Peptide Mapping ◽  
Two Dimensional ◽  
Major Event ◽  
Mouse Epidermis ◽  
Cell Stage ◽  
Processing Steps

A major event in the keratinization of epidermis is the production of the histidine-rich protein filaggrin (26,000 mol wt) from its high molecular weight (greater than 350,000) phosphorylated precursor (profilaggrin). We have identified two nonphosphorylated intermediates (60,000 and 90,000 mol wt) in NaSCN extracts of epidermis from C57/Bl6 mice by in vivo pulse-chase studies. Results of peptide mapping using a two-dimensional technique suggest that these intermediates consist of either two or three copies of filaggrin domains. Each of the intermediates has been purified. The ratios of amino acids in the purified components are unusual and essentially identical. The data are discussed in terms of a precursor containing tandem repeats of similar domains. In vivo pulse-chase experiments demonstrate that the processing of the high molecular weight phosphorylated precursor involves dephosphorylation and proteolytic steps through three-domain and two-domain intermediates to filaggrin. These processing steps appear to occur as the cell goes through the transition cell stage to form a cornified cell.

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