scholarly journals Early pregnancy peripheral blood gene expression and risk of preterm delivery: a nested case control study

2009 ◽  
Vol 9 (1) ◽  
Author(s):  
Daniel A Enquobahrie ◽  
Michelle A Williams ◽  
Chunfang Qiu ◽  
Seid Y Muhie ◽  
Kimberly Slentz-Kesler ◽  
...  
Keyword(s):  
Gene Expression ◽  
Preterm Delivery ◽  
Early Pregnancy ◽  
Peripheral Blood ◽  
Case Control Study ◽  
Case Control ◽  
Blood Gene Expression ◽  
Peripheral Blood Gene Expression ◽  
Nested Case Control ◽  
Control Study

Chronic HPA activity in mothers with preterm delivery: A pilot nested case-control study

2019 ◽  
pp. 1-9
Author(s):  
B. Gelaye ◽  
C. Kirschbaum ◽  
Q.-Y. Zhong ◽  
S.E. Sanchez ◽  
M.B. Rondon ◽  
...  
Keyword(s):  
Preterm Delivery ◽  
Case Control Study ◽  
Case Control ◽  
Nested Case Control ◽  
Control Study

scholarly journals Trimethylamine N-Oxide Metabolites in Early Pregnancy and Risk of Gestational Diabetes: A Nested Case-Control Study

2019 ◽  
Vol 104 (11) ◽  
pp. 5529-5539 ◽  
Author(s):  
Xiaoxu Huo ◽  
Jing Li ◽  
Yun-Feng Cao ◽  
Sai-Nan Li ◽  
Ping Shao ◽  
...  
Keyword(s):  
Gestational Diabetes ◽  
Early Pregnancy ◽  
Case Control Study ◽  
Conditional Logistic Regression ◽  
Full Range ◽  
Case Control ◽  
Conversion Ratio ◽  
Additive Interaction ◽  
Nested Case Control ◽  
Control Study

Abstract Objectives This study aimed to investigate the associations between trimethylamine N-oxide (TMAO) and related metabolites in early pregnancy and the risk of gestational diabetes mellitus (GDM). Design A prospective cohort of 22,302 pregnant women from 2010 to 2012 in Tianjin, China, was used to perform a nested case-control study. A total of 243 women with GDM and 243 women without GDM matched by maternal age (±1 year) were used as cases and controls, respectively. Conditional logistic regression and restricted cubic spline were used to examine the full-range risk associations between individual TMAOs metabolites at the first antenatal care visit with GDM. Trimethylamine conversion ratio (TMAR) was defined as trimethylamine (TMA)/its precursors, and trimethylamine N-oxide conversion ratio (TMAOR) was defined as TMAO/TMA. An additive interaction between high TMAR and low TMAOR indicates a state of TMA accumulation, and a mathematical interaction between high TMAR and high TMAOR indicates accumulation of TMAO. Results TMA was linearly associated with GDM, whereas TMA precursors and TMAO were inversely associated with GDM with clear threshold effects, i.e., 16 nmol/mL for TMAO, 200 nmol/mL for betaine, 112 nmol/mL for l-carnitine, and 110 and 270 nmol/mL for cholinechloride (a U-shaped relationship). Copresence of TMAR >0.35 and TMAOR ≤0.15 was associated with a markedly higher OR (11.16; 95% CI, 5.45 to 22.8), compared with TMAR >0.35 only (OR = 1.71; 95% CI, 0.42 to 6.95) or TMAOR ≤0.15 only (OR = 2.06; 95% CI, 1.09 to 3.90), with a significant additive interaction. However, the mathematical interaction was nonsignificant. Conclusions TMAO metabolites in the early pregnancy were associated with the risk of GDM, whereas TMA was more likely to play a causal role in GDM.

DNA Methylation in Peripheral Blood and Risk of Gastric Cancer: A Prospective Nested Case–control Study

2020 ◽  
Author(s):  
James A Chamberlain ◽  
Pierre-Antoine Dugué ◽  
Julie K. Bassett ◽  
Roger L. Milne ◽  
Jihoon E Joo ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Dna Methylation ◽  
Peripheral Blood ◽  
Case Control Study ◽  
Case Control ◽  
Nested Case Control ◽  
Control Study

scholarly journals Early Pregnancy Sex Steroids and Maternal Breast Cancer: A Nested Case–Control Study

2014 ◽  
Vol 74 (23) ◽  
pp. 6958-6967 ◽  
Author(s):  
Renée T. Fortner ◽  
Helena Schock ◽  
Rudolf Kaaks ◽  
Matti Lehtinen ◽  
Eero Pukkala ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Early Pregnancy ◽  
Sex Steroids ◽  
Case Control Study ◽  
Case Control ◽  
Nested Case Control ◽  
Control Study

scholarly journals Association of Toll-like receptor 7 variants with life-threatening COVID-19 disease in males: findings from a nested case-control study

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Chiara Fallerini ◽  
Sergio Daga ◽  
Stefania Mantovani ◽  
Elisa Benetti ◽  
Nicola Picchiotti ◽  
...  
Keyword(s):  
Gene Expression ◽  
Case Control Study ◽  
Susceptibility Gene ◽  
Case Control ◽  
Type I ◽  
Loss Of Function ◽  
Extreme Phenotype ◽  
Male Patients ◽  
Nested Case Control ◽  
Control Study

Background: Recently, loss-of-function variants in TLR7 were identified in two families in which COVID-19 segregates like an X-linked recessive disorder environmentally conditioned by SARS-CoV-2. We investigated whether the two families represent the tip of the iceberg of a subset of COVID-19 male patients. Methods: This is a nested case-control study in which we compared male participants with extreme phenotype selected from the Italian GEN-COVID cohort of SARS-CoV-2-infected participants (<60y, 79 severe cases versus 77 control cases). We applied the LASSO Logistic Regression analysis, considering only rare variants on young male subsets with extreme phenotype, picking up TLR7 as the most important susceptibility gene. Results: Overall, we found TLR7 deleterious variants in 2.1% of severely affected males and in none of the asymptomatic participants. The functional gene expression profile analysis demonstrated a reduction in TLR7-related gene expression in patients compared with controls demonstrating an impairment in type I and II IFN responses. Conclusion: Young males with TLR7 loss-of-function variants and severe COVID-19 represent a subset of male patients contributing to disease susceptibility in up to 2% of severe COVID-19.

scholarly journals Early Pregnancy Peripheral Blood DNA Methylation and Risk of Gestational Diabetes Mellitus: A Nested Case-Control Study

2021 ◽  
Author(s):  
Xiaolei Wang ◽  
Jin Huang ◽  
Sisi Long ◽  
Huijun Lin ◽  
Na Zhang ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Dna Methylation ◽  
Peripheral Blood ◽  
Case Control Study ◽  
Methylation Status ◽  
First Trimester ◽  
Case Control ◽  
Cpg Sites ◽  
Nested Case Control ◽  
Control Study

Abstract Introduction: Genome-wide DNA methylation profiling has been used to identify CpG sites relevant to gestational diabetes mellitus (GDM). However, these sites have not been verified in larger samples. Here, our aim was to evaluate the changes in target CpG sites in the peripheral blood of pregnant women with GDM in their first trimester. Research Design and Methods: This nested case-control study examined a large cohort of women with GDM in early pregnancy (10–15 weeks; n = 80). Target CpG sites were extracted from related published literature and bioinformatics analysis. The DNA methylation levels at 337 CpG sites located in 27 target genes were determined using MethylTarget™ sequencing. The best cut-off levels for methylation of CpG sites were determined using the generated ROC curve. The independent effect of CpG site methylation status on GDM was analyzed using conditional logistic regression. Results Methylation levels at 6 CpG sites were significantly higher in the GDM group than in controls, whereas those at 7 CpG sites were significantly lower (P < 0.05). The area under the ROC curve at each methylation level of the significant CpG sites ranged between 0.593 and 0.650 for GDM prediction. After adjusting for possible confounders, the hypermethylation status of candidate sites cg68167324 (OR = 3.168, 1.038–9.666) and cg24837915 (OR = 5.232, 1.659–16.506) was identified as more strongly associated with GDM; conversely, the hypermethylation of sites cg157130156 (OR = 0.361, 0.135–0.966) and cg89438648 (OR = 0.206, 0.065–0.655) might indicate lower risk of GDM. Conclusions The methylation status of target CpG sites in the peripheral blood of pregnant women during the first trimester is associated with GDM pathogenesis, and has potential as a predictor of GDM.

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