scholarly journals SEM and TEM for identification of capsular fibrosis and cellular behavior around breast implants – a descriptive analysis

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Britta Kuehlmann ◽  
Isabel Zucal ◽  
Clark Andrew Bonham ◽  
Lydia-Marie Joubert ◽  
Lukas Prantl
Keyword(s):  
Descriptive Analysis ◽  
Breast Implants ◽  
Foreign Body Response ◽  
Future Research ◽  
Cellular Mechanisms ◽  
Tem Analysis ◽  
Capsular Fibrosis ◽  
Sem And Tem ◽  
Post Operation ◽  
Bacterial Accumulation

Abstract Background Capsular fibrosis (CF) is the most common long-term complication in implant-based breast augmentation. It is well accepted that the foreign body response (FBR) instigates the development of fibrotic disease. Our study aims to compare murine and human samples of CF and describe the cellular and extracellular matrix (ECM) composition using scanning and transmission electron microscopy (SEM and TEM). Results Miniature microtextured silicone breast implants were implanted in mice and subsequently harvested at days 15, 30, and 90 post-operation. Isolated human capsules with the most aggravated form of CF (Baker IV) were harvested post-operation. Both were analyzed with SEM and TEM to assess cellular infiltration and ECM structure. An architectural shift of collagen fiber arrangement from unidirectional to multidirectional was observed at day 90 when compared to days 15 and 30. Fibrosis was observed with an increase of histiocytic infiltration. Moreover, bacterial accumulation was seen around silicone fragments. These findings were common in both murine and human capsules. Conclusions This murine model accurately recapitulates CF found in humans and can be utilized for future research on cellular invasion in capsular fibrosis. This descriptive study helps to gain a better understanding of cellular mechanisms involved in the FBR. Increases of ECM and cellularity were observed over time with SEM and TEM analysis.

scholarly journals SEM and TEM for Identification of Capsular Fibrosis and Cellular Behavior Around Breast Implants – a Descriptive Analysis

2021 ◽  
Author(s):  
Britta Kuehlmann ◽  
Isabel Zucal ◽  
Clark Andrew Bonham ◽  
Lydia-Marie Joubert ◽  
Lukas Prantl
Keyword(s):  
Descriptive Analysis ◽  
Breast Implants ◽  
Foreign Body Response ◽  
Future Research ◽  
Cellular Mechanisms ◽  
Tem Analysis ◽  
Sem And Tem ◽  
Transmission Electron ◽  
Post Operation ◽  
Bacterial Accumulation

Abstract BackgroundCapsular fibrosis (CF) is the most common long-term complication in implant-based breast augmentation. It is well accepted that the foreign body response (FBR) instigates the development of fibrotic disease. Our study aims to compare murine and human samples of CF and describe the cellular and extracellular matrix (ECM) composition using scanning and transmission electron microscopy (SEM and TEM).ResultsMiniature microtextured silicone breast implants were implanted in mice and subsequently harvested at days 15, 30, and 90 post-operation. Isolated human capsules with the most aggravated form of CF (Baker IV) were harvested post-operation. Both were analyzed with SEM and TEM to assess cellular infiltration and ECM structure.An architectural shift of collagen fiber arrangement from unidirectional to multidirectional was observed at day 90 when compared to days 15 and 30. Fibrosis was observed with an increase of histiocytic infiltration. Moreover, bacterial accumulation was seen around silicone fragments. These findings were common in both murine and human capsules.ConclusionsThis murine model accurately recapitulates CF found in humansand can be utilized for future research on cellular invasion in capsular fibrosisThis descriptive study helps to gain a better understanding of cellular mechanisms involved in the FBR. Increases of ECM and cellularity were observed over time with SEM and TEM analysis.

A Review of Minimum Quantity Lubrication (MQL) based on Bibliometry

2020 ◽  
Vol 13 ◽  
Author(s):  
Gaurav Gaurav ◽  
Abhay Sharma ◽  
G S Dangayach ◽  
M L Meena
Keyword(s):  
Descriptive Analysis ◽  
Minimum Quantity Lubrication ◽  
Citation Network ◽  
Machining Process ◽  
Cutting Fluid ◽  
Future Research ◽  
Research Directions ◽  
Minimum Quantity ◽  
Research Areas ◽  
Future Research Directions

Background: Minimum quantity lubrication (MQL) is one of the most promising machining techniques that can yield a reduction in consumption of cutting fluid more than 90 % while ensuring the surface quality and tool life. The significance of the MQL in machining makes it imperative to consolidate and analyse the current direction and status of research in MQL. Objective: This study aims to assess global research publication trends and hot topics in the field of MQL among machining process. The bibliometric and descriptive analysis are the tools that the investigation aims to use for the data analysis of related literature collected from Scopus databases. Methods: Various performance parameters are extracted, such as document types and languages of publication, annual scientific production, total documents, total citations, and citations per article. The top 20 of the most relevant and productive sources, authors, affiliations, countries, word cloud, and word dynamics are assessed. The graphical visualisation of the bibliometric data is presented in terms of bibliographic coupling, citation, and co-citation network. Results: The investigation reveals that the International Journal of Machine Tools and Manufacture (2611 citations, 31 hindex) is the most productive journal that publishes on MQL. The most productive institution is the University of Michigan (32 publications), the most cited country is Germany (1879 citations), and the most productive country in MQL is China (124 publications). The study shows that ‘Cryogenic Machining’, ‘Sustainable Machining’, ‘Sustainability’, ‘Nanofluid’ and ‘Titanium alloy’ are the most recent keywords and indications of the hot topics and future research directions in the MQL field. Conclusion: The analysis finds that MQL is progressing in publications and the emerging with issues that are strongly associated with the research. This study is expected to help the researchers to find the most current research areas through the author’s keywords and future research directions in MQL and thereby expand their research interests.

Ways that nurse practitioner students self-explain during diagnostic reasoning

Diagnosis ◽  
2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Leah Burt ◽  
Susan Corbridge ◽  
Colleen Corte ◽  
Laurie Quinn ◽  
Lorna Finnegan ◽  
...  
Keyword(s):  
Problem Solving ◽  
Health Care Providers ◽  
Nurse Practitioner ◽  
Descriptive Analysis ◽  
Diagnostic Errors ◽  
Diagnostic Reasoning ◽  
Knowledge Structures ◽  
Future Research ◽  
Biological Knowledge ◽  
Care Providers

Abstract Objectives An important step in mitigating the burden of diagnostic errors is strengthening diagnostic reasoning among health care providers. A promising way forward is through self-explanation, the purposeful technique of generating self-directed explanations to process novel information while problem-solving. Self-explanation actively improves knowledge structures within learners’ memories, facilitating problem-solving accuracy and acquisition of knowledge. When students self-explain, they make sense of information in a variety of unique ways, ranging from simple restatements to multidimensional thoughts. Successful problem-solvers frequently use specific, high-quality self-explanation types. The unique types of self-explanation present among nurse practitioner (NP) student diagnosticians have yet to be explored. This study explores the question: How do NP students self-explain during diagnostic reasoning? Methods Thirty-seven Family NP students enrolled in the Doctor of Nursing Practice program at a large, Midwestern U.S. university diagnosed three written case studies while self-explaining. Dual methodology content analyses facilitated both deductive and qualitative descriptive analysis. Results Categories emerged describing the unique ways that NP student diagnosticians self-explain. Nine categories of inference self-explanations included clinical and biological foci. Eight categories of non-inference self-explanations monitored students’ understanding of clinical data and reflect shallow information processing. Conclusions Findings extend the understanding of self-explanation use during diagnostic reasoning by affording a glimpse into fine-grained knowledge structures of NP students. NP students apply both clinical and biological knowledge, actively improving immature knowledge structures. Future research should examine relationships between categories of self-explanation and markers of diagnostic success, a step in developing prompted self-explanation learning interventions.

A Descriptive Analysis of Music Therapy Employment from 2013 to 2019

2020 ◽  
Author(s):  
Jonathon Oden
Keyword(s):  
Music Therapy ◽  
Descriptive Analysis ◽  
American Music ◽  
High Rate ◽  
Future Research ◽  
Full Time ◽  
Music Therapists ◽  
Funding Sources ◽  
Part Time ◽  
Therapy Services

Abstract The purpose of this study was to descriptively analyze music therapy employment data from 2013 to 2019, including years in the field, gender, age, ethnicity, hours worked, jobs created, number of new board-certified music therapists (MT-BCs), funding sources, and wages. A database was created to analyze descriptive data from the 2013–2019 American Music Therapy Association Workforce Analysis Surveys as well as data from the Certification Board for Music Therapists. Results indicate a large portion of music therapists (MTs) have been in the field for five years or less. Though the majority of MTs work full time, there is a high rate of part-time employment. An estimate of the total number of new full-time jobs represented a ratio of 57% of new MT-BCs during the period. Private pay was the most reported funding source for music therapy services. Music therapy wages tended to be higher for those with higher levels of education. Limitations of the study and suggestions for future research are provided.

Moral cosmopolitanism, civic action and ethical consumption: Social differences in young Australians’ global citizenship

2021 ◽  
pp. 144078332110538
Author(s):  
Quentin Maire
Keyword(s):  
Sociological Theory ◽  
Global Citizenship ◽  
Descriptive Analysis ◽  
Statistical Modelling ◽  
Ethical Consumption ◽  
Future Research ◽  
Social Differences ◽  
Theory Of Practice ◽  
Positive Attitudes ◽  
Citizenship Identity

The relationship between global citizenship identity and actions remains an unsettled issue. In this article we use the PISA 2018 survey to explore whether global citizenship identity is associated with a greater likelihood of engaging in ‘globally minded’ practices among young Australians. Descriptive analysis reveals that self-reported global citizenship identity is associated with higher levels of moral cosmopolitanism, more so than with greater intercultural values or with more positive attitudes to immigrants. However, this self-declared identity does not necessarily translate into cosmopolitan action. Statistical modelling shows that, in the Australian context, global citizenship knowledge, values and identity account for a limited proportion of differences in self-reported cosmopolitan action. We argue that a sociological theory of practice helps explain these results. We conclude by highlighting future research opportunities to better understand the social determinants of global citizenship practices, including by exploring out-of-school socialisation and a broader range of cosmopolitan practices.

scholarly journals Demographic and clinical characteristics of individuals’ Intentions to attend cardiac rehabilitation following hospitalization for heart failure: a descriptive analysis

2021 ◽  
Author(s):  
Kristen Cunningham
Keyword(s):  
Heart Failure ◽  
Pilot Study ◽  
Cardiac Rehabilitation ◽  
Clinical Characteristics ◽  
Descriptive Analysis ◽  
Future Research ◽  
Post Discharge ◽  
Convenience Sample ◽  
Rehabilitation Centers ◽  
Quantitative Descriptive

There is limited literature available that addresses heart failure patient attendance at cardiac rehabilitation centers. This quantitative descriptive pilot study used a convenience sample (n=30) to determine differences in socio-demographic and clinical characteristics and complications among individuals with heart failure who intended or did not intend to attend cardiac rehabilitation six weeks post-discharge. Findings suggest those intending to attend were significantly (p<0.05) older, unemployed/retired, received an income >$50,000, were able to drive, had lower functional classification scores, and experienced fewer complications over six weeks post-discharge. This descriptive pilot study provides an understanding of factors associated with intention to attend cardiac rehabilitation as well as the feasibility of the study design and procedures. Implications focus on strategies to increase potential attendance at cardiac rehabilitation in the heart failure population at the health care provider, organizational and policy levels as well as areas for future research.

scholarly journals Research fronts of Chemical Biology

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Shanshan Lv
Keyword(s):  
High Throughput Screening ◽  
Drug Targets ◽  
Chemical Biology ◽  
Rational Design ◽  
Future Research ◽  
Design Strategies ◽  
Binding Assays ◽  
Cellular Mechanisms ◽  
Diagnostic Technologies

Abstract Over the past decades, researchers have witnessed substantially increasing and ever-growing interests and efforts in Chemical Biology studies, thanks to the development of genome and epi-genome sequencing (revealing potential drug targets), synthetic chemistry (producing new medicines), bioorthogonal chemistry (chemistry in living systems) and high-throughput screening technologies (in vitro cell systems, protein binding assays and phenotypic assays). This report presents literature search results for current research in Chemical Biology, to explore basic principles, summarize recent advances, identify key challenges, and provide suggestions for future research (with a focus on Chemical Biology in the context of human health and diseases). Chemical Biology research can positively contribute to delivering a better understanding of the molecular and cellular mechanisms that accompany pathology underlying diseases, as well as developing improved methods for diagnosis, drug discovery, and therapeutic delivery. While much progress has been made, as shown in this report, there are still further needs and opportunities. For instance, pressing challenges still exist in selecting appropriate targets in biological systems and adopting more rational design strategies for the development of innovative and sustainable diagnostic technologies and medical treatments. Therefore, more than ever, researchers from different disciplines need to collaborate to address the challenges in Chemical Biology.

scholarly journals Extracellular vesicles from amyloid-β exposed cell cultures induce severe dysfunction in cortical neurons

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Chiara Beretta ◽  
Elisabeth Nikitidou ◽  
Linn Streubel-Gallasch ◽  
Martin Ingelsson ◽  
Dag Sehlin ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Cortical Neurons ◽  
Amyloid Β ◽  
Neuronal Cell ◽  
Time Lapse ◽  
Cellular Mechanisms ◽  
Axonal Swelling ◽  
Tem Analysis ◽  
Neuronal Cell Bodies ◽  
Time Lapse Imaging

AbstractAlzheimer’s disease (AD) is characterized by a substantial loss of neurons and synapses throughout the brain. The exact mechanism behind the neurodegeneration is still unclear, but recent data suggests that spreading of amyloid-β (Aβ) pathology via extracellular vesicles (EVs) may contribute to disease progression. We have previously shown that an incomplete degradation of Aβ42 protofibrils by astrocytes results in the release of EVs containing neurotoxic Aβ. Here, we describe the cellular mechanisms behind EV-associated neurotoxicity in detail. EVs were isolated from untreated and Aβ42 protofibril exposed neuroglial co-cultures, consisting mainly of astrocytes. The EVs were added to cortical neurons for 2 or 4 days and the neurodegenerative processes were followed with immunocytochemistry, time-lapse imaging and transmission electron microscopy (TEM). Addition of EVs from Aβ42 protofibril exposed co-cultures resulted in synaptic loss, severe mitochondrial impairment and apoptosis. TEM analysis demonstrated that the EVs induced axonal swelling and vacuolization of the neuronal cell bodies. Interestingly, EV exposed neurons also displayed pathological lamellar bodies of cholesterol deposits in lysosomal compartments. Taken together, our data show that the secretion of EVs from Aβ exposed cells induces neuronal dysfunction in several ways, indicating a central role for EVs in the progression of Aβ-induced pathology.

Green competencies: insights and recommendations from a systematic literature review

2020 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Clement Cabral ◽  
Rajib Lochan Dhar
Keyword(s):  
Literature Review ◽  
Systematic Literature Review ◽  
Thematic Analysis ◽  
Descriptive Analysis ◽  
Future Research ◽  
Research Directions ◽  
Content Type ◽  
Classification Framework ◽  
Future Research Directions ◽  
Research Gap

PurposeThis study conceptualises the construct – green competencies. The concept is in the niche stage and needs further elaboration. Hence, to address the research gap, this study follows the steps proposed by Tranfield et al. (2003). The major part of the study comprises descriptive analysis and thematic analysis. Descriptive analysis of the selected 66 articles was examined with the classification framework, which contains year-wise distribution, journal-wise distribution, the focus of the concept, the economic sector, and dimensions of sustainable development. The paper conducts a thematic analysis of the following research questions. What are the green competencies and their conceptual definition? What are their dimensions?Design/methodology/approachThis paper applies a systematic literature review of green competencies literature, extends the state-of-the-art using the natural resource-based view, and discusses future research directions for academicians and practitioners.FindingsIn recent years, there was considerable interest in green competencies (GC), as reflected in the surge of articles published in this genre. This paper asserts that green competencies are a multidimensional construct comprised of green knowledge, green skills, green abilities, green attitudes, green behaviours, and green awareness.Originality/valueDespite the significance of green competencies, there has been a dearth of study to define the constructs and identify the dimensions. Hence, this study addresses the literature gap by conceptualisation and discusses dimensions of the construct.

Customer acceptance of ride-hailing in Indonesia

2020 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Mohammad Nabil Almunawar ◽  
Muhammad Anshari ◽  
Syamimi Ariff Lim
Keyword(s):  
Online Survey ◽  
Descriptive Analysis ◽  
Small Sample ◽  
Future Research ◽  
Adoption Studies ◽  
Enabling Factors ◽  
Broadband Wireless ◽  
Content Type ◽  
Customer Acceptance ◽  
Use Of Technology

Purpose The purpose of this paper is to investigate the enabling factors and the customers’ acceptance of ride-hailing in Indonesia. Design/methodology/approach The authors adopt some constructs from the unified theory of acceptance and use of technology (UTAUT) 2 as the framework for the study to derive factors that influence the acceptance of ride-hailing in Indonesia. Samples through a convenience sampling method were collected from an online survey and were transformed into data through coding and subsequently processed using SPSS for descriptive analysis, reliability test, correlation and multiple regression analysis for hypothesis testing. Findings Ride-hailing started in 2015 in Indonesia. Five enabling factors make digital ride-hailing possible, the internet, smartphone, broadband wireless network, digital map and global positioning system. The authors found that performance expectancy, social influence and habit positively influence customers to accept ride-hailing in Indonesia. Research limitations/implications Although this research has a small sample, it is still relevant to understand people’s acceptance to the ride-hailing platform. As a ride-hailing platform is now transformed to a multisided markets platform, adoption studies or other studies on each market to cover the whole picture of the platform influence to the society, and its contribution to the national economy will be very interesting. The authors’ future research will cover various services covered by ride-hailing companies. Originality/value This study proposes and argues that four main enabling factors make digital ride-hailing a viable business. The study contributes to three significant factors that influence the acceptance of ride-hailing in Indonesia.

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