Dual species transcript profiling during the interaction between banana (Musa acuminata) and the fungal pathogen Fusarium oxysporum f. sp. cubense

ABSTRACTEarly detection for Fusarium wilt resistant of banana plants can be done at seedling phase under greenhouse condition. The purpose of early detection was to screen and evaluate some number of local and hybrid accession of banana plants for resistance to Fusarium oxysporum f. sp. cubense (Foc VCG 01213/16 TR4 or Foc TR4). The treatments were done by immersing the root seedling in Fusarium inoculant at concentration of 106 conidia mL-1, for 2 hours, then planted in sterile sand media and placed in container with "double tray system". Experiments were performed using 15 accessions of banana i.e. 1 accession of wild musa (Musa acuminata var. malaccesis), 7 local accessions and 7 hybrid bananas. Based on greenhouse bioassay, banana Rejang#2 (AA) showed resistance to Foc TR4. The results were consistent in the field condition. Tetraploid plants for pisang Rejang#2 (AAAA) also showed resistant to Foc TR4. Cultivar Cavendish (AAA) and Madu (AA) showed highly susceptible; Ustrali (AAAB) and Goroho (AAA) showed susceptible to Foc TR4. Wild banana (Musa acuminata var. malaccensis) and Mas Jambe 4x (AAAA) showed moderate susceptible. Of the total 7 hybrids accession; 3 hybrids showed moderate susceptible, and 4 other hybrids showed susceptible to Foc TR4.Keywords: banana plants, early detection, Foc TR4, hybrid, local ABSTRAK Deteksi awal tingkat ketahananan tanaman pisang terhadap penyakit layu Fusarium oxysporum f. sp. cubense (Foc) dapat dilakukan pada masa pembibitan di rumah kaca. Penelitian bertujuan untuk evaluasi awal tingkat ketahanan beberapa aksesi pisang liar, pisang lokal dan pisang hasil persilangan terhadap penyakit layu Fusarium. Akar bibit pisang pada air yang berisi inokulan Fusarium (Foc VCG 01213/16 atau Foc TR4) pada konsentrasi 106 konidia mL-1, selama 2 jam yang selanjutnya ditanam pada media pasir steril dan diletakkan pada kontainer dengan pengaturan “double tray system”. Lima belas aksesi pisang yang terdiri atas 1 aksesi pisang liar (Musa acuminata var. malaccensis), 7 aksesi pisang budidaya dan 7 nomor pisang hasil persilangan dievaluasi tingkat ketahanan terhadap Foc TR4. Dari hasil pengujian di rumah kaca, pisang Rejang#2 (AA) menunjukkan status tahan terhadap penyakit layu Fusarium Foc VCG 01213/16 TR4 yang hasilnya sesuai dan stabil dengan tingkat ketahanannya di lapangan. Pisang Rejang#2 tetraploid (AAAA) juga menunjukkan status tahan terhadap Foc TR4. Pisang Madu (AA) dan Cavendish (AAA) menunjukkan hasil sangat rentan, sedangkan pisang Goroho (AAA) dan pisang Ustrali (AAAB) menunjukkan status rentan. Pisang liar Musa acuminata var. malaccensis (AA) dan pisang Mas Jambe 4x (AAAA) menunjukkan status agak rentan. Pada seleksi pisang hasil persilangan, didapatkan 3 nomor pisang hibrid dengan status agak rentan yakni 816 MDRK, 1060 MDRK, 2284 MMRK, sedangkan 4 nomor hibrid lainnya menunjukkan status rentan.Kata kunci: deteksi dini, Foc TR4, hibrid, lokal, pisang

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DEVELOPMENT OF A GENETIC LINKAGE MAP FOR GENES ASSOCIATED WITH RESISTANCE AND SUSCEPTIBILITY TO FUSARIUM OXYSPORUM F. SP. CUBENSE FROM AN F1 HYBRID POPULATION OF MUSA ACUMINATA SSP. MALACCENSIS

Acta Horticulturae ◽

10.17660/actahortic.2009.828.34 ◽

2009 ◽

pp. 333-340 ◽

Cited By ~ 5

Author(s):

F. Kayat ◽

N. Bonar ◽

R. Waugh ◽

S. Rajinder ◽

A.R. Rahimah ◽

...

Keyword(s):

Fusarium Oxysporum ◽

Linkage Map ◽

Genetic Linkage ◽

Genetic Linkage Map ◽

Musa Acuminata ◽

Hybrid Population ◽

F1 Hybrid

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Long noncoding RNAs that respond to Fusarium oxysporum infection in ‘Cavendish’ banana (Musa acuminata)

Scientific Reports ◽

10.1038/s41598-017-17179-3 ◽

2017 ◽

Vol 7 (1) ◽

Cited By ~ 11

Author(s):

Wenbin Li ◽

Chunqiang Li ◽

Shuxia Li ◽

Ming Peng

Keyword(s):

Fusarium Oxysporum ◽

Noncoding Rnas ◽

Musa Acuminata ◽

Long Noncoding Rnas ◽

Cavendish Banana

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Selection for resistance to Fusarium wilt in red clover

Canadian Journal of Plant Science ◽

10.4141/p98-005 ◽

1999 ◽

Vol 79 (3) ◽

pp. 351-356 ◽

Cited By ~ 2

Author(s):

B. C. Venuto ◽

R. R. Smith ◽

C. R. Grau

Keyword(s):

Fusarium Oxysporum ◽

Fungal Pathogen ◽

Trifolium Pratense ◽

Red Clover ◽

Severity Index ◽

Research Station ◽

Vascular Wilt ◽

Trifolium Pratense L ◽

Selection For ◽

Overall Resistance

In Wisconsin, Fusarium oxysporum, Schlect., a pathogen causing vascular wilt, is the most prevalent fungal pathogen recovered from diseased red clover (Trifolium pratense L.) plants. This study was conducted to determine the mode of inheritance for red clover resistance to this pathogen and to develop resistant germplasm. Virulent isolates of this pathogen, collected from red clover plants at the Ashland Research Station, Ashland, Wisconsin, were used to screen three populations, the red clover cultivars Arlington and Marathon and the C11 germplasm, for resistant plants. Plants were inoculated with the pathogen and evaluated for reaction, using a disease-severity index (DSI) score from 1 to 5 (1 = no reaction, 5 = plant dead). Selected plants from each cycle were intercrossed to produce subsequent generations. After two and three cycles of selection, the developed populations were simultaneously evaluated for gain from selection. The gain from selection for resistance in these populations (cycle 0 minus cycle 2) ranged from 0.31 to 0.48, 0.12 to 0.75, and 0.13 to 0.83 DSI units, respectively, for Arlington, Marathon, and C11. Estimated narrow-sense heritabilities, based on cycle-1 and cycle-2 progeny, were, respectively, 0.20 and 0.37 for Arlington, 0.15 and 0.13 for Marathon, and 0.06 and 0.17 for C11. These results indicate that resistance is a quantitative trait controlled by many loci, each contributing some portion to overall resistance in the host. Key words: Red clover, Trifolium pratense L., Fusarium oxysporum, vascular wilt, resistance

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Standardized bioassays: An improved method for studying Fusarium oxysporum f. sp. cubense race 4 (FocR4) pathogen stress response in Musa acuminata cv. ‘Berangan’

Asia Pacific Journal of Molecular Biology and Biotechnology ◽

10.35118/apjmbb.2019.027.3.10 ◽

2019 ◽

pp. 101-112

Author(s):

Yusmin Mohd-Yusuf ◽

Norzulaani Khalid ◽

Jameel R. Al-Obaidi ◽

Nadiya Akmal Baharum ◽

Kamilatulhusna Zaidi ◽

...

Keyword(s):

Fusarium Oxysporum ◽

Musa Acuminata ◽

Post Inoculation ◽

Molecular Approach ◽

Disease Response ◽

Pathogenesis Related ◽

Bioassay Experiment ◽

Infection Parameters ◽

Race 4 ◽

Leaf Symptoms

To date, there is no standardized Fusarium bioassay protocol established owing partly to the wide variety of Fusarium oxysporum f. sp. cubense (Foc) isolates and banana cultivars present. Thus, validation of the infection parameters is deemed essential prior to each bioassay experiment. In the current study, a simple standardized workflow was developed based on available assays for testing Fusarium wilt disease response in Musa acuminata using M. acuminata cv. ‘Berangan’ of tissue-culture origin as a model. The phenotypic assays were able to detect external disease symptoms less than one week post-inoculation, while the molecular approach using RT-qPCR identified differential expression of catalase (CAT), pathogenesis-related 10 (PR10), phenylalanine ammonia-lyase (PAL) and xylanase (XYL) genes as early as day 0. The transcript levels of PR10 and XYL fluctuated over 4 days of Foc Race 4 (FocR4 C1 HIR isolate) infection while the expression of CAT steadily increased over time. In contrast, PAL was highly upregulated at 2 days post-inoculation. These signature changes suggest that all genes tested might be involved in the early defense response of ‘Berangan’ plants against FocR4 infection. ‘Berangan’ cultivar was found to be highly susceptible to Foc Race 4 (C1 HIR isolate) with leaf symptoms index (LSI) and rhizome discoloration index (RDI) scores of 4.257 and 5.971, respectively. The procedure elaborated in this study can be used as a reference Foc bioassay for reproducible and comparable results possibly across cultivars and test isolates due to its simple steps aided by integration of phenotypic and molecular approach.

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178 Differences in Grape Phylloxera-related Grapevine Root Damage in Organically and Conventionally Managed Vineyards in California

HortScience ◽

10.21273/hortsci.34.3.472f ◽

1999 ◽

Vol 34 (3) ◽

pp. 472F-473 ◽

Cited By ~ 2

Author(s):

D.W. Lotter ◽

J. Granett ◽

A.D. Omer

Keyword(s):

Organic Matter ◽

Fusarium Oxysporum ◽

Fungal Pathogen ◽

Fungal Pathogens ◽

Secondary Infection ◽

Soil Characteristics ◽

Root Damage ◽

Significant Difference ◽

Root Necrosis ◽

Organically Managed

Secondary infection of roots by fungal pathogens is a primary cause of vine damage in phylloxera-infested grapevines. In summer and fall surveys in 1997 and 1998, grapevine root samples were taken from organically managed vineyards (OMVs) and from conventionally managed vineyards (CMVs), all of which were phylloxera-infested. In both years, root samples from OMVs showed significantly less fungal pathogen-caused root necrosis than samples from CMVs, averaging 9% in OMVs and 31% in CMVs. There was no significant difference in phylloxera populations per 100 g of root between OMVs and CMVs, although there was a trend toward higher populations in CMVs. Soil characteristics, percent organic matter, total nitrogen, nitrate, and percent sand/silt/clay were not significantly different between the two regimes. Cultures of necrotic root tissue showed significantly higher levels of the benefical fungus Trichoderma in OMVs in 1997 but not in 1998, and there were significantly higher levels of the pathogens Fusarium oxysporum and Cylindrocarpon spp. in CMVs in 1998 but not in 1997. Implications for further research and viticulture are discussed.

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Fusarium Oxysporum Disrupts Microbiome-Metabolome Networks in Arabidopsis Thaliana Roots

10.21203/rs.3.rs-845639/v1 ◽

2021 ◽

Author(s):

Enoch Narh Kudjordjie ◽

Kourosh Hooshmand ◽

Rumakanta Sapkota ◽

Inge S. Fomsgaard ◽

Mogens Nicolaisen

Keyword(s):

Arabidopsis Thaliana ◽

Fusarium Oxysporum ◽

Fungal Pathogen ◽

Plant Disease ◽

Differential Resistance ◽

Amplicon Sequencing ◽

Interactive Effects ◽

Pathogen Invasion ◽

Plant Disease Control ◽

Microbiome Assembly

Abstract BackgroundAlthough it is well established that plant metabolomes mediate microbiome assembly, the question of how metabolome-microbiome interactions may prevent pathogen invasion remains to be answered. To address this question, we studied microbiome and metabolome profiles of two Arabidopsis thaliana accessions, Columbia-0 (Col-0) and Landsberg erecta (Ler-0) with differential resistance profiles to the fungal pathogen Fusarium oxysporum f.sp. mathioli (FOM). We used amplicon sequencing to characterize bacterial (16S) and fungal (ITS2) communities, and we used targeted metabolite analysis across 5 stages of FOM host progression. ResultsWe found that microbiome and metabolome profiles were markedly altered in FOM-inoculated and non-inoculated samples of resistant Col-0 and susceptible Ler-0. Co-occurrence network analysis revealed robust microbial networks in the resistant Col-0 compared to the susceptible Ler-0, during FOM infection. Specific metabolites and microbial OTUs (including indicator and hub OTUs) correlated in both non-inoculated and inoculated Col-0 and Ler-0. The glucosinolates 4-hydroxyglucobrassicin, neoglucobrassicin and indole-3 carbinol, but also phenolic compounds were active in structuring the A. thaliana-microbiome. ConclusionsOur results highlight the interactive effects of host resistance and its associated microbiota on Fusarium infection and progression. These findings shed significant insights into plant inter-omics dynamics during pathogen invasion and could possibly facilitate the exploitation of microbiomes for plant disease control.

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Identification and characterization of miRNA169 family members in banana (Musa acuminata L.) that respond to fusarium oxysporum f. sp. cubense infection in banana cultivars

PeerJ ◽

10.7717/peerj.6209 ◽

2018 ◽

Vol 6 ◽

pp. e6209 ◽

Cited By ~ 10

Author(s):

Shun Song ◽

Yi Xu ◽

Dongmei Huang ◽

Muhammad Aleem Ashraf ◽

Jingyang Li ◽

...

Keyword(s):

Fusarium Oxysporum ◽

Fusarium Wilt ◽

Family Members ◽

Musa Acuminata ◽

Small Rna Sequencing ◽

Transcriptional Level ◽

Evolutionary Analysis ◽

Multiple Sequence ◽

Expression Levels ◽

Starting Point

MicroRNAs (miRNAs) play an important role in plant resistance to pathogen infections. However, little is known about the role of miRNAs in banana Fusarium wilt, which is the most economically devastating disease in banana production. In the present study, we identified and characterized a total of 18 miR169 family members in banana (Musa acuminata L.) based on small RNA sequencing. The banana miR169 family clustered into two groups based on miRNA evolutionary analysis. Multiple sequence alignment indicated a high degree of sequence conservation in miRNA169 family members across 28 plant species. Computational target prediction algorithms were used to identify 25 targets of miR169 family members in banana. These targets were enriched in various metabolic pathways that include the following molecules: glycine, serine, threonine, pentose, glycerolipids, nucleotide sugars, starch, and sucrose. Through miRNA transcriptomic analysis, we found that ma-miR169a and ma-miR169b displayed high expression levels, whereas the other 16 ma-miR169 members exhibited low expression in the HG and Baxi banana cultivars. Further experiments indicate that there were negative relationships between ma-miR169a, ma-miR169b and their targets basing on their expression levels to Foc4 (Fusarium oxysporum f. sp. cubense tropical race 4) infection in resistant cultivars. But they were low expressed in susceptive cultivars. These results suggested that the expression levels of ma-miR169a and ma-miR169b were consistent with the resistance degree of the banana cultivars to Foc4. The analysis presented here constitutes a starting point to understand ma-miR169-mediated Fusarium wilt resistance at the transcriptional level in banana and predicts possible candidate targets for the genetic improvement of banana resistance to Foc4.

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