scholarly journals Serum CXCL10/IP-10 may be a potential biomarker for severe Mycoplasma pneumoniae pneumonia in children

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Mengyao Li ◽  
Ying Chen ◽  
Huihan Li ◽  
Dehua Yang ◽  
Yunlian Zhou ◽  
...  
Keyword(s):  
Clinical Practice ◽  
Mycoplasma Pneumoniae ◽  
Area Under The Curve ◽  
Clinical Information ◽  
Blood Samples ◽  
Cytokines And Chemokines ◽  
Potential Biomarker ◽  
Inflammatory Chemokines ◽  
The Relationship ◽  
Mycoplasma Pneumoniae Pneumonia

Abstract Background How to early distinguish the severity of Mycoplasma pneumoniae pneumonia (MPP) is a worldwide concern in clinical practice. We therefore conducted this study to assess the relationship between levels of serum inflammatory chemokines and the severity of MPP. Methods In this prospective study, we enrolled 39 children with MPP, whose clinical information was collected, blood samples were assayed for cytokines and chemokines by ELISA. Results The levels of serum CXCL10 in children with severe MPP were significantly higher than those in children with mild MPP (2500.0 [1580.9–2500.0] vs. 675.7 [394.7–1134.9], P < 0.001). Measurement of CXCL10 levels in serum enabled the differentiation of children with severe MPP with an area under the curve (AUC) of 0.885 (95 % CI 0.779–0.991, P < 0.001), with a sensitivity of 81.0 % and a specificity of 83.3 %. Conclusions Serum CXCL10 level may be a potential biomarker for severe MPP in children.

scholarly journals Altered distribution of NK cell subsets in children with Mycoplasma pneumoniae pneumonia Altered distribution of NK cell subsets in children with Mycoplasma pneumoniae pneumonia

2020 ◽  
Author(s):  
Weifang Zhou ◽  
Dandan Zhang ◽  
Xiuling Cao ◽  
Yuqin Li ◽  
Ying Ding ◽  
...  
Keyword(s):  
Nk Cells ◽  
Mycoplasma Pneumoniae ◽  
Peripheral Blood ◽  
Nk Cell ◽  
Respiratory Illness ◽  
Absolute Number ◽  
Healthy Controls ◽  
Blood Samples ◽  
The Relationship ◽  
Mycoplasma Pneumoniae Pneumonia

Abstract Background : Mycoplasma pneumoniae pneumonia (MPP) is a common respiratory illness in children, particularly those with allergic constitutions. NK cells and cytokines are thought to be involved; however, understanding of the immunopathology of MPP is incomplete. Methods : Peripheral blood samples were collected from 51 children hospitalized with with MPP, 26 with an allergic constitution and 25 without, and 29 healthy controls. NK cell subsets were analyzed by flow cytometry and the expression of interleukin (IL)-1 alpha and IL-18 was detected by ELISA. The relationship between NK cell subsets and the expression of IL-18 and IL-1 alpha was determined. Results : The number of CD3 − CD56 + NK cells and CD3 − CD56 dim CD16 bright NK cells in children with MPP was lower than in healthy controls (P < 0.05). The percentage of CD3 − CD56 + NK cells, CD3 − CD56 dim CD16 bright NK cells and the number of CD3 − CD56 dim CD16 bright NK cells in the MPP allergic group were lower than in the non-allergic group (P < 0.05). The expression of IL-18 was significantly increased in the MPP groups (P < 0.05), and the absolute number of CD3 − CD56 dim CD16 bright NK cells negatively correlated with IL-18 levels in the peripheral blood (P < 0.05). Conclusion : Reduced numbers of NK cell subsets were identified in children with MPP and MPP with an allergic predisposition compared with healthy controls. Concomitant increases in IL-18 in children with MPP suggest the involvement of IL-18 in the immunopathogenesis of MPP and may be related to the reduced CD3 − CD56 dim CD16 bright NK cells.

scholarly journals Microbiologic and Immunologic Evaluation of a Single High Dose of Azithromycin for Treatment of Experimental Mycoplasma pneumoniae Pneumonia

2005 ◽  
Vol 49 (9) ◽  
pp. 3970-3973 ◽  
Author(s):  
Ana María Ríos ◽  
Mónica Fonseca-Aten ◽  
Asunción Mejías ◽  
Susana Chávez-Bueno ◽  
Kathy Katz ◽  
...  
Keyword(s):  
Mycoplasma Pneumoniae ◽  
High Dose ◽  
Single High Dose ◽  
Cytokines And Chemokines ◽  
Lung Histopathology ◽  
Mycoplasma Pneumoniae Pneumonia

ABSTRACT We evaluated the efficacy of azithromycin therapy given as a single high dose or divided over 5 days for the treatment of mild experimental Mycoplasma pneumoniae pneumonia. Although both azithromycin regimens significantly reduced quantitative cultures, lung histopathology, and pulmonary cytokines and chemokines, there were no significant differences between the two regimens.

scholarly journals Elevation of serum alpha-1-acid glycoprotein in children with bronchial pneumonia caused by Mycoplasma pneumoniae infection

2020 ◽  
Author(s):  
Huanying Fang ◽  
Aiping Zhou ◽  
shusheng yang
Keyword(s):  
Mycoplasma Pneumoniae ◽  
Area Under The Curve ◽  
Related Factor ◽  
Related Factors ◽  
Receiver Operating Characteristic Curves ◽  
Acid Glycoprotein ◽  
Inflammatory Conditions ◽  
Laboratory Examinations ◽  
Logistic Regressions ◽  
The Relationship

Abstract Background:Alpha-1-acid glycoprotein(AGP) is an acute phase protein which can be elevated under inflammatory conditions. Few studies have explored the use of monitoring serum AGP in Mycoplasma pneumoniae pneumonia(MPP). Methods:We aimed to evaluate the relationship between serum AGP and other convention inflammation bio-markers,in order to explore its clinical significance in children with MPP. Pediatric inpatients with bronchopneumonia from January to December 2018 were enrolled and divided into two groups -children with MPP and children without MPP severed as controls. Serum AGP, procalcitonin (PCT) ,and other laboratory examinations were compared and multiple logistic regressions analysis was used to select the main related factors, then receiver-operating characteristic curves were used to evaluate the value of the selected markers used to identify MPP from bronchopneumonia. Results: Two hundred and sixty-eight children were enrolled in this study.Among them, 87 (32.46%) cases were diagnosed as MPP and 181 gender-matched children were without MPP. Children with MPP were slightly older than those without MPP and had lower levels of serum PCT (P<0.05). In addition, serum AGP was significantly higher in patients with MPP than control participants (136.06±46.37 vs. 122.29±44.21,respectively;P=0.020). Multiple logistic regressions analysis adjusted for age showed that only AGP was the main related factor ,which could perhaps be used to identify MPP from common bronchopneumonia and its area under the curve (AUC) was 0.703(95% CI: 0.634-0.772;P=0.000).Combination with AGP and PCT could slightly improve the AUC(95 % CI) to 0.732 (95% CI: 0.666-0.797, P=0.000). Conclusion: AGP is perhaps a potential bio-marker to distinguish MPP from other bronchopneumonia in children and assist early diagnosis and treatment as soon as possible for it is relatively convenient and fast to be carried out.

scholarly journals The clinical significance of IL-6s and IL-27s in Bronchoalveolar lavage fluids from children with mycoplasma pneumoniae pneumonia

2020 ◽  
Author(s):  
Jie Zhao ◽  
Yuyun Li ◽  
Wen Zhang
Keyword(s):  
Bronchoalveolar Lavage ◽  
Mycoplasma Pneumoniae ◽  
Mixed Infection ◽  
Single Infection ◽  
Infection Group ◽  
Patient Groups ◽  
Infection Types ◽  
And Control ◽  
The Relationship ◽  
Mycoplasma Pneumoniae Pneumonia

Abstract Backgrounds: IL-6 is associated with the severity of mycoplasma pneumoniae pneumonia (MPP). But the relationship between IL-27 and MPP was unknown. Methods: 98 patients with MPP who were under 14 years old, were enrolled in this study and divided into mild cases and severe cases, Mycoplasma pneumoniae (MP) single infection group and MP mixed infection group, and low MP DNA loads group and high MP DNA loads group, by severity, infection types and DNA loads respectively. 15 children with foreign bodies for bronchus were also enrolled as control. IL-6s and IL-27s in bronchoalveolar lavage fluids (BALFs) from these children were measured by ELISA. Results: There were significant differences in IL-6s of BALFs from patients between mild cases and severe cases, MP single infection group and MP mixed infection group, and low MP DNA loads group and high MP DNA loads group, respectively (P﹤0.05). Compared with IL-6s of BALFs from control, IL-6 in BALFs from the 5 patient groups were significantly higher (P﹤0.05) except that from low MP DNA loads group. IL-27s in BALFs from MP mixed infection group were significantly lower than those from MP single infection group and control (P﹤0.05) respectively. Conclusion: IL-6 was firmly associated with MPP and had potential application in clinical practice while IL-27 was not related to MP infection.

scholarly journals Salivary IL-6 Concentration Is Associated with Frailty Syndrome in Older Individuals

Diagnostics ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 117
Author(s):  
Pablo Gómez-Rubio ◽  
Isabel Trapero ◽  
Omar Cauli ◽  
Cristina Buigues
Keyword(s):  
Characteristic Curve ◽  
Area Under The Curve ◽  
Activity Level ◽  
Frailty Syndrome ◽  
Older Individuals ◽  
Cross Sectional ◽  
Potential Biomarker ◽  
Syndrome Individual ◽  
The Relationship ◽  
Frailty Criteria

Background: One of the physiological changes that is most closely associated with frailty is the increase in pro-inflammatory cytokines, and IL-6 in particular. Most studies have demonstrated this association using blood samples. We analyzed the relationship between frailty syndrome, individual frailty criteria, and IL-6 levels obtained by saliva tests. Methods: A cross-sectional pilot study was performed among women institutionalized in nursing homes. Frailty was defined as having three or more of the following components: low lean mass, weakness, self-reported exhaustion, low activity level, and slow walking speed; prefrailty was defined as having one or two of those components. Results: There was a significant and positive correlation between the frailty score and salivary IL-6 concentration. Regarding the associations between IL-6 and individual dichotomized frailty criteria, there were significant differences in salivary IL-6 concentration in two frailty criteria: weight loss (p = 0.002) and low physical activity (p = 0.007). Receiver operating characteristic curve analysis showed that IL-6 concentration significantly (p < 0.05) (although moderately) discriminated patients that progressed in the frailty syndrome (the area under the curve value was 0.697 with 95% CI 0.566–0.827). Conclusions: Salivary IL-6 concentration can be used as potential biomarker of frailty syndrome and as a tool to monitor the effects of interventions in frail individuals.

scholarly journals Complement C1q as a Potential Biomarker for Obesity and Metabolic Syndrome in Chinese Adolescents

2020 ◽  
Vol 11 ◽  
Author(s):  
Xuelian Yang ◽  
Yanan Ma ◽  
Zhongyi Zhao ◽  
Shihan Zhen ◽  
Deliang Wen
Keyword(s):  
Metabolic Syndrome ◽  
Operating Characteristic ◽  
Area Under The Curve ◽  
Chinese Adolescents ◽  
Trend Test ◽  
Roc Curve Analysis ◽  
Potential Biomarker ◽  
Prevalence Of Obesity ◽  
Complement C1q ◽  
The Relationship

BackgroundComplement C1q (C1q) has been confirmed to be related to obesity, metabolic syndrome (MetS), and its components. However, human data regarding the associations are relatively scarce. This study aimed to investigate associations of C1q with obesity as well as MetS in Chinese adolescents.MethodsA total of 1,191 Chinese adolescents aged 13–18 years were enrolled in this study. The biochemical and anthropometric variables of all the subjects were evaluated using standardized procedures. C1q was measured using the immunoturbidometric assay. The relationship between C1q and obesity or MetS was analyzed using multiple regression analyses.ResultsObesity was more prevalent among participants in the highest tertile than in the lowest tertile of C1q levels. The highest tertile of C1q was related to a greater effect on the risk of MetS, and its trend test was statistically significant. Except for hyperglycemia, the prevalence of other components of MetS significantly increased relative to an increase in C1q tertile. Receiver operating characteristic (ROC) curve analysis of C1q for predicting adolescents with MetS illustrated that the area under the curve (AUC) was 0.82 [95% confidence interval (CI): 0.76, 0.88; P&lt;0.001] in the total population after adjusting for confounders.ConclusionsThis study observed a significantly higher prevalence of obesity and MetS features in adolescents with high C1q. The findings of the current study also reported a significant relationship between C1q levels and MetS components [except for fasting plasma glucose (FPG)] in Chinese adolescents. C1q may represent a biomarker for predicting obesity or MetS in adolescents.

scholarly journals The clinical significance of IL-6s and IL-27s in Bronchoalveolar lavage fluids from children with mycoplasma pneumoniae pneumonia

2020 ◽  
Author(s):  
Jie Zhao ◽  
Yuyun Li ◽  
Wen Zhang
Keyword(s):  
Bronchoalveolar Lavage ◽  
Mycoplasma Pneumoniae ◽  
Mixed Infection ◽  
Single Infection ◽  
Infection Group ◽  
Patient Groups ◽  
Infection Types ◽  
And Control ◽  
The Relationship ◽  
Mycoplasma Pneumoniae Pneumonia

Abstract Backgrounds: IL-6 is associated with the severity of mycoplasma pneumoniae pneumonia (MPP). But the relationship between IL-27 and MPP was unknown. Methods: 98 patients with MPP < 14 years old were enrolled in this study and divided into groups by severity (mild cases and severe cases), infection types (MP single infection group and MP mixed infection group) and DNA loads (low MP DNA loads group and high MP DNA loads group), respectively. 15 children with foreign bodies in bronchus were also enrolled as control. IL-6s and IL-27s in bronchoalveolar lavage fluids (BALFs) from these children were measured by ELISA. Results: There were significant differences in IL-6s of BALFs from patients between mild cases and severe cases, MP single infection group and MP mixed infection group, and low MP DNA loads group and high MP DNA loads group, respectively (P﹤0.05). Compared with IL-6s of BALFs from control, IL-6 in BALFs from the 6 patient groups were significantly higher (P﹤0.05). IL-27s in BALFs from MP mixed infection group were significantly lower than those from MP single infection group and control (P﹤0.05) respectively. Conclusion: IL-6 was firmly associated with MPP and had potential application in clinical practice while IL-27 was not related to MP infection.

scholarly journals Application of Lactoferrin andα1-Antitrypsin in Gingival Retention Fluid to Diagnosis of Periodontal Disease

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Ryosuke Koshi ◽  
Kazuhiko Kotani ◽  
Mariko Ohtsu ◽  
Naoto Yoshinuma ◽  
Naoyuki Sugano
Keyword(s):  
Clinical Practice ◽  
Periodontal Disease ◽  
Area Under The Curve ◽  
Pocket Depth ◽  
Bleeding On Probing ◽  
Periodontal Status ◽  
Positive Correlation ◽  
Probing Pocket Depth ◽  
The Relationship

Objectives.Periodontal disease is prevalent and has an inflammation associated with not only oral but also systemic pathologies. The diagnosis by biomarkers is required for clinical practice on periodontal disease. The lactoferrin andα1-antitrypsin were both inflammation-related molecules. The present study investigated the relationship between the periodontal status and the two biomarkers in gingival retention fluid (GRF).Patients and Methods. In 63 subjects with periodontitis, the GRF was sampled from maxillary anterior gingiva using a microbrush for 30 seconds. The lactoferrin andα1-antitrypsin levels in GRF were measured by an enzyme-link solvent immunoassay. Periodontal status was evaluated by probing pocket depth (PD) and bleeding on probing (BOP).Results. There was a higher level of these biomarkers in saliva (median (ng/mL), lactoferrin: 3611.9,α1-antitrypsin: 4573.3) than in GRF (lactoferrin: 61.0,α1-antitrypsin: 54.7). There was a mild-to-moderate but significantly positive correlation in lactoferrin orα1-antitrypsin between GRF and saliva. There was a positively mild-to-moderate accuracy (area under the curve: 0.60–0.81) of lactoferrin orα1-antitrypsin in GRF or in saliva to distinguish the severity of periodontal status. The cutoff level (ng/mL) of lactoferrin in GRF for detecting ≥30% of PD ≥ 4 mm (moderate periodontitis) was 68.6 and for detecting ≥20% of BOP (clinically active periodontitis) was 61.2. The cutoff level (ng/mL) ofα1-antitrypsin in GRF for detecting ≥30% of PD ≥ 4 mm was 54.5 and for detecting ≥20% of BOP was 35.3.Conclusions.The data can promote an application of the measurements of lactoferrin andα1-antitrypsin in GRF to clinical practice on periodontal disease.

Correlation between Vitamin A Deficiency and the Severity of Mycoplasma Pneumoniae Pneumonia in Children

2021 ◽  
Vol 7 (5) ◽  
pp. 3364-3373
Author(s):  
Teng Wang ◽  
Ping Zheng ◽  
Qian Jiang ◽  
Qianqian Sun
Keyword(s):  
Vitamin A ◽  
Mycoplasma Pneumoniae ◽  
Detection Rate ◽  
Vitamin A Deficiency ◽  
Hospitalized Children ◽  
Immunoglobulin Level ◽  
Normal Ratio ◽  
The Relationship ◽  
Immunoglobulin Levels ◽  
Mycoplasma Pneumoniae Pneumonia

Mycoplasma pneumoniae pneumonia is a problem that has attracted the attention of children’s respiratory department in recent years. The relationship between vitamin A deficiency (VA) and the severity of Mycoplasma disease (MPP) in children was studied. In this paper, the hospitalized children with Mycoplasma pneumoniae pneumonia (MPP) in our hospital were selected as the research objects, and they were divided into common type and refractory type (RMPP) for comparative experiment. The VA and immunoglobulin levels of the two groups were compared. Attention should be paid to the normal ratio of VA deficiency (CVAD) and sub deficiency (svad) in the two groups. In this paper, the relationship between infection, immunoglobulin level and VA level was analyzed. The results showed that CVAD was 27.27% in MPP group and 63.75% in RMPP group. Comparison of immunoglobulin levels between the two groups: the levels of IgM, IgA and IgG in the normal MPP group were significantly lower than those in the RMPP group. On the other hand, the detection rate of CVAD was 81.48% in RMPP with infection and 54.72% in RMPP without infection. The detection rate of CVAD in patients with infection was significantly higher than that in patients without infection. There was a correlation between VA Deficiency and MPP classification.

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