scholarly journals Proper positioning of mice for Cobb angle radiographic measurements

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Zhe Yi Chen ◽  
Keith Dip Kei Luk ◽  
You Qiang Song ◽  
Bo Gao ◽  
Jason Pui Yin Cheung

Abstract Background There is no recommended standard for positioning of a mouse for radiographic assessment of the spine. This is necessary to have reproducible radiographic data and avoid false positive results. The objective of this study was to investigate the impact of various postures on Cobb angle measurements and to set up a positioning standard for imaging mouse spines. Methods This study was conducted in three parts. Firstly, we identified the problem of lack of posture standardization for radiographs. We collected 77 C57BL/6 J mice for spine radiographs and found a scoliosis prevalence of 28.6% with large variations in curve magnitude. Secondly, 24 C57BL/6 J mice underwent 4 consecutive weekly radiographs and observed high variations (relative standard deviation: 125.3%) between radiographs. Thirdly, we collected another 82 C57BL/6 J mice and designed 14 different postures that could take place during imaging. These postures were related to curling of the limbs, and head, pelvic and tail tilting. Results The results showed that head and pelvic tilting significantly affects the curve magnitude with effect size (Glass’s delta) over 1.50. Avoiding these incorrect positions during radiographs is warranted. The standard recommended posture for mouse imaging entails positioning the snout, interorbital space, neck and whole spine in one line, and with the limbs placed symmetrical to the trunk, whilst avoiding stretching the body of the mouse. Conclusions Our work exemplified the importance of standard protocol during imaging when using an animal model in the scoliosis study. We recommend utilizing this standard in studying various disorders of the spine to avoid technical causes for the appearance of a curve.

2020 ◽  
Author(s):  
Zhe Yi Chen ◽  
Keith Dip Kei Luk ◽  
You Qiang Song ◽  
Bo Gao ◽  
Jason Pui Yin Cheung

Abstract BackgroundThere is no recommended standard for positioning of a mouse for radiographic assessment of the spine. This is necessary to have reproducible radiographic data and avoid false positive results. The objective of this study was to investigate the impact of various postures on Cobb angle measurements and to set up a positioning standard for imaging mouse spines. MethodsThis study was conducted in three parts. Firstly, we identified the problem of lack of posture standardization for radiographs. We collected 77 C57BL/6J mice for spine radiographs and found a scoliosis prevalence of 28.6% with large variations in curve magnitude. Secondly, 24 C57BL/6J mice underwent 4 consecutive weekly radiographs and observed high variations (relative standard deviation: 125.3%) between radiographs. Thirdly, we collected another 82 C57BL/6J mice and designed 14 different postures that could take place during imaging. These postures were related to curling of the limbs, and head, pelvic and tail tilting. ResultsThe results showed that head and pelvic tilting significantly affects the curve magnitude with effect size (Glass’s delta) over 1.50. Avoiding these incorrect positions during radiographs is warranted. The standard recommended posture for mouse imaging entails positioning the snout, interorbital space, neck and whole spine in one line, and with the limbs placed symmetrical to the trunk, whilst avoiding stretching the body of the mouse. ConclusionsOur work exemplified the importance of standard protocol during imaging when using animal model in the scoliosis study. We recommend utilizing this standard in studying various disorders of the spine.


2020 ◽  
Vol 102-B (2) ◽  
pp. 254-260 ◽  
Author(s):  
Jason P. Y. Cheung ◽  
Prudence W. H. Cheung

Aims The aim of this study was to assess whether supine flexibility predicts the likelihood of curve progression in patients with adolescent idiopathic scoliosis (AIS) undergoing brace treatment. Methods This was a retrospective analysis of patients with AIS prescribed with an underarm brace between September 2008 to April 2013 and followed up until 18 years of age or required surgery. Patients with structural proximal curves that preclude underarm bracing, those who were lost to follow-up, and those who had poor compliance to bracing (<16 hours a day) were excluded. The major curve Cobb angle, curve type, and location were measured on the pre-brace standing posteroanterior (PA) radiograph, supine whole spine radiograph, initial in-brace standing PA radiograph, and the post-brace weaning standing PA radiograph. Validation of the previous in-brace Cobb angle regression model was performed. The outcome of curve progression post-bracing was tested using a logistic regression model. The supine flexibility cut-off for curve progression was analyzed with receiver operating characteristic curve. Results A total of 586 patients with mean age of 12.6 years (SD 1.2) remained for analysis after exclusion. The baseline Cobb angle was similar for thoracic major curves (31.6° (SD 3.8°)) and lumbar major curves (30.3° (SD 3.7°)). Curve progression was more common in the thoracic curves than lumbar curves with mean final Cobb angles of 40.5° (SD 12.5°) and 31.8° (SD 9.8°) respectively. This dataset matched the prediction model for in-brace Cobb angle with less mean absolute error in thoracic curves (0.61) as compared to lumbar curves (1.04). Reduced age and Risser stage, thoracic curves, increased pre-brace Cobb angle, and reduced correction and flexibility rates predicted increased likelihood of curve progression. Flexibility rate of more than 28% has likelihood of preventing curve progression with bracing. Conclusion Supine radiographs provide satisfactory prediction for in-brace correction and post-bracing curve magnitude. The flexibility of the curve is a guide to determine the likelihood for brace success. Cite this article: Bone Joint J 2020;102-B(2):254–260.


2021 ◽  
Author(s):  
◽  
Margaret Medlyn

<p>Writers from diverse disciplines have rhapsodised over the impact of the operatic voice on the listener, while musicologists such as Abbate, Duncan, and Risi have explored the effect that concepts of voice and bodily engagement have had on our critical readings of opera. Yet although perspectives on performance have become an increasingly vital aspect of operatic criticism, no one has laid out how opera singers experience performance in relation to the ideas of embodiment that scholars write about. The discourse on embodiment and voice is theoretical; most discussions of female voice can be mapped on to any historical period and on to any voice, so that all voices end up being treated the same; paradoxically, in addition it is a discourse that largely omits the body.  Indeed, the complexity of connecting many different layers of mind and bodily engagement, that is, the embodiment, is a task that requires detailed and specialised training. Without attempting to speak for all singers at all times, I propose that by acknowledging that different singers achieve and think about particular elements of embodiment in different ways, we can start to come to terms with an individual singer’s creative agency, as a co-creator of the composer’s music.  In this dissertation I outline key characteristics of the type of embodied voice that has become the basis of operatic singing today, how that operatic voice is produced in performance, and the importance of the singer’s own bodily engagement in making that sound and constituting the performance itself. By juxtaposing operatic criticism and readings of voice and vocality with an interrogation of my own physical engagement in singing a few particular roles (as a singer specialising in nineteenth and twentieth-century operatic repertoire), I demonstrate how a singer “creates” roles. My detailed analyses illustrate how a singer’s fully conscious bodily engagement, in and through the breath, is inextricably linked with musical and dramatic interpretation, and sets up the vocal spectacle and embodied agency so central to our modern experience of opera.  Moreover, in the context of specific readings of particular operatic roles, I argue that particular composers set up specific ways in which singers manipulate elements of body and mind – so that the score can influence and even control how a singer can or cannot breathe. As I will demonstrate in detailed studies of four roles by Verdi and Wagner (all of which I have sung in performance), some scores set up an almost physical collaboration between the singer herself and the way in which text, breath and music are shaped and moulded in performance by particular features of the vocal writing. While a large number of roles could be explored in those terms, the demands placed upon body and voice are individual and each role of every opera is always distinct; Verdi and Wagner roles provide particularly valuable examples because of the complex intersection between a rich psychological framework for interpretative engagement and a complex vocal and bodily collaboration. In addition, my focus on a particular timeframe in the historical development of vocal practice suggests fascinating correlations with the case studies I discuss from Il trovatore, Die Walküre and Parsifal. The new type of singer developing the skills and voice to sing these roles predicates today’s vocal and stage practices that in turn have influenced my own experience. Offering an in-depth examination of the complex tasks an opera singer undertakes, I also examine differences in the vocality in singing Wagner and Verdi roles, culminating in a detailed exposition of my chosen roles.  This dissertation, therefore, sets up a complex picture of the ways in which vocal performance is constructed and controlled by Verdi and Wagner, on the one hand, and how particular scores also set up the conditions that allow singers in these texts to unleash their voice to achieve “wildness” and expression that lies beyond the text. Through these case studies, I establish a discourse of vocality that allows detailed readings of aspects of vocal performance that seemingly bypass rational communication. In the end, I build a case for understanding how singers’ embodiment contributes to the creativity of the performance in ways hitherto intuited but not analysed. Thus I offer a counterbalance and reinterpretation of traditional perspectives on the reality of performance, addressing singers and scholars alike.</p>


Author(s):  
Mehlika İşcan ◽  
Burcu Kılıç ◽  
Akif Turna ◽  
Mehmet Kamil Kaynak

Abstract OBJECTIVES The Nuss technique comprises the placement of an intrathoracic bar behind the sternum. However, besides improving the body posture through the correction of the pectus excavatum (PE), this procedure may cause or worsen thoracic scoliosis as a result of the considerable stress loaded on the chest wall and the thorax. Our goal was to investigate the impact of the Nuss procedure on the thoracic spinal curvature in patients with PE. METHODS A total of 100 patients with PE who underwent the Nuss procedure were included in the study and evaluated retrospectively. The Haller index (HI), asymmetry index and sternal torsion angle were calculated from thoracic computed tomography images before the operation. To evaluate the scoliosis in the T2–T8 thoracic vertebrae, Cobb angles were calculated on a plain chest X-ray before the Nuss operation and after the removal of the bar. Cobb angles were classified as normal (5°), scoliotic posture (5°–10°) and scoliosis (&gt;10°). All angles before and after the Nuss operation were compared. The patients were followed up for a mean of 41 months. Substernal bars were removed after a mean of 33 months. RESULTS The mean age of the patients was 19.6 ± 6.7 years. The Cobb angle was statistically significantly increased in all patients (P = 0.01), male patients (P = 0.01) and children (P = 0.046) but not in adults (P = 0.11) and female patients (P = 0.54). The Cobb angle was increased in patients with severe (HI ≥ 3.5) but not in patients with moderate (3.2 &lt; HI &lt; 3.5) or mild (2.0 &lt; HI &lt; 3.2) PE deformity. CONCLUSIONS The present study shows that the Cobb angle indicates that the severity of thoracic scoliosis increases following the Nuss procedure, particularly in male patients, in patients with mild and moderate sternal torsion angle and in those with a high preoperative HI. This alteration might be due to correctional forces and torque applied by the bar. Patients undergoing the Nuss procedure for the correction of PE should be followed up strictly for timely diagnosis and management of the scoliosis.


2020 ◽  
Vol 4 (1) ◽  
pp. 504-515
Author(s):  
Keniche Assia

Background:Wilson’s illness, or hepatolenticular degeneration, is an autosomal passive issue that outcomes from an accumulation of copper prevalently in the liver and brain. The aggregation is happen because of imperfect biliary discharge of copper. Current information demonstrates that grown-up people need to ingest about 0.75 mg of copper every day to continue a parity. Because of a hereditary imperfection, people with Wilson’s ailment can't discharge the abundance copper, bringing about a progressive develop of copper in the body. Substantial metals are the most hurtful of the concoction toxins and are of specific worry because of their poison levels to people. From this poisonous metal copper, which has impacts in drinking water on the gastrointestinal tract, yet there is some with respect to the drawn out impacts of copper on delicate populaces, for example, bearers of the quality for Wilson’smalady and other metabolic issue of copper homeostasis. Method:We picked as a characteristic adsorbent in this work the Moringa Oleifera Seeds (MOSs) from Mali assortment, which could be a practical and earth safe strategy for water sanitization. All examinations were directed at room temperature, andafter being permitted to represent two hours, the examples were broke down utilizing the Atomic Absorption Spectrophotometer (AAS). The seeds of Moringawere set up by taking around 1 g of the seed squashed and blending it in with around 20 cm3 of water to be decontaminated in three distinct measuring utensils. Result:The different arrangements after filtration, indicated an expanded thickness in the amount of copper, which demonstrates the viability of the cleansing intensity of the seeds of MO. The best consequences of adsorption were for 30 min of contact time. We had half copper evacuation. The impact of contact time is significant in biosorption investigations to decide the harmony time required for the take-up of metal particles by the MOSs. Conclusion:In this investigation, the MOS from Mali were demonstrated to be a proficient regular adsorbent material of copper from fluid arrangements. The limit of the MOS to evacuate copper relies upon numerous models as; plant assortment and states of examinations. Our examination is going on the investigation of evacuation parameters, for example, contact time, pH, temperature, molecule size, sorbent portion, and introductory metal fixation.


2021 ◽  
Author(s):  
◽  
Margaret Medlyn

<p>Writers from diverse disciplines have rhapsodised over the impact of the operatic voice on the listener, while musicologists such as Abbate, Duncan, and Risi have explored the effect that concepts of voice and bodily engagement have had on our critical readings of opera. Yet although perspectives on performance have become an increasingly vital aspect of operatic criticism, no one has laid out how opera singers experience performance in relation to the ideas of embodiment that scholars write about. The discourse on embodiment and voice is theoretical; most discussions of female voice can be mapped on to any historical period and on to any voice, so that all voices end up being treated the same; paradoxically, in addition it is a discourse that largely omits the body.  Indeed, the complexity of connecting many different layers of mind and bodily engagement, that is, the embodiment, is a task that requires detailed and specialised training. Without attempting to speak for all singers at all times, I propose that by acknowledging that different singers achieve and think about particular elements of embodiment in different ways, we can start to come to terms with an individual singer’s creative agency, as a co-creator of the composer’s music.  In this dissertation I outline key characteristics of the type of embodied voice that has become the basis of operatic singing today, how that operatic voice is produced in performance, and the importance of the singer’s own bodily engagement in making that sound and constituting the performance itself. By juxtaposing operatic criticism and readings of voice and vocality with an interrogation of my own physical engagement in singing a few particular roles (as a singer specialising in nineteenth and twentieth-century operatic repertoire), I demonstrate how a singer “creates” roles. My detailed analyses illustrate how a singer’s fully conscious bodily engagement, in and through the breath, is inextricably linked with musical and dramatic interpretation, and sets up the vocal spectacle and embodied agency so central to our modern experience of opera.  Moreover, in the context of specific readings of particular operatic roles, I argue that particular composers set up specific ways in which singers manipulate elements of body and mind – so that the score can influence and even control how a singer can or cannot breathe. As I will demonstrate in detailed studies of four roles by Verdi and Wagner (all of which I have sung in performance), some scores set up an almost physical collaboration between the singer herself and the way in which text, breath and music are shaped and moulded in performance by particular features of the vocal writing. While a large number of roles could be explored in those terms, the demands placed upon body and voice are individual and each role of every opera is always distinct; Verdi and Wagner roles provide particularly valuable examples because of the complex intersection between a rich psychological framework for interpretative engagement and a complex vocal and bodily collaboration. In addition, my focus on a particular timeframe in the historical development of vocal practice suggests fascinating correlations with the case studies I discuss from Il trovatore, Die Walküre and Parsifal. The new type of singer developing the skills and voice to sing these roles predicates today’s vocal and stage practices that in turn have influenced my own experience. Offering an in-depth examination of the complex tasks an opera singer undertakes, I also examine differences in the vocality in singing Wagner and Verdi roles, culminating in a detailed exposition of my chosen roles.  This dissertation, therefore, sets up a complex picture of the ways in which vocal performance is constructed and controlled by Verdi and Wagner, on the one hand, and how particular scores also set up the conditions that allow singers in these texts to unleash their voice to achieve “wildness” and expression that lies beyond the text. Through these case studies, I establish a discourse of vocality that allows detailed readings of aspects of vocal performance that seemingly bypass rational communication. In the end, I build a case for understanding how singers’ embodiment contributes to the creativity of the performance in ways hitherto intuited but not analysed. Thus I offer a counterbalance and reinterpretation of traditional perspectives on the reality of performance, addressing singers and scholars alike.</p>


2018 ◽  
Vol 15 (2) ◽  
pp. 1-20
Author(s):  
Sabri Embi ◽  
Zurina Shafii

The purpose of this study is to examine the impact of Shariah governance and corporate governance (CG) on the risk management practices (RMPs) of local Islamic banks and foreign Islamic banks operating in Malaysia. The Shariah governance comprises the Shariah review (SR) and Shariah audit (SA) variables. The study also evaluates the level of RMPs, CG, SR, and SA between these two type of banks. With the aid of SPSS version 20, the items for RMPs, CG, SR, and SA were subjected to principal component analysis (PCA). From the PCA, one component or factor was extracted each for the CG, SR, and RMPs while another two factors were extracted for the SA. Primary data was collected using a self-administered survey questionnaire. The questionnaire covers four aspects ; CG, SR, SA, and RMPs. The data received from the 300 usable questionnaires were subjected to correlation and regression analyses as well as an independent t-test. The result of correlation analysis shows that all the four variables have large positive correlations with each other indicating a strong and significant relationship between them. From the regression analysis undertaken, CG, SR, and SA together explained 52.3 percent of the RMPs and CG emerged as the most influential variable that impacts the RMPs. The independent t-test carried out shows that there were significant differences in the CG and SA between the local and foreign Islamic banks. However, there were no significant differences between the two types of the bank in relation to SR and RMPs. The study has contributed to the body of knowledge and is beneficial to academicians, industry players, regulators, and other stakeholders.


2020 ◽  
pp. 15-18
Author(s):  
Inna R. Kilmetova ◽  
◽  
Igor A. Rodin ◽  
Nazira I. Khayrullina ◽  
Nikolay G. Fenchenko ◽  
...  

Summary. The disbalanced feeding and the uneven distribution of micro- and macroelements in the environment leads to a trace element, in particular hypomelanosis. To accelerate the growth and preservation of young farm animals include in the diet of various biological additives and drugs, which include selenium. For stimulation of weight gain in the livestock industry, as well as for the prevention and treatment of pathological processes in addition to micro - and macrouse amino acids, primarily methionine. The aim of this work was to study the influence of composition of DAFS-25+Polizon on morpho-biochemical parameters of blood and functional state of the liver in fattening bulls of black-motley breed in the conditions of the Republic of Bashkortostan. Experiments using were conducted on bull-calves of black-motley breed of the properties in the properties age from 6 to 15 months. The first experimental group during the experiment was additionally given the composition of DAFS-25+Polizon at a dose of 2 mg/kg, the animals of the control group received a standard diet. To assess the impact of the composition DAFS-25+Polizon on metabolism cattle studied morphological and biochemical indicators of blood and conducted histological examination of the liver. It is established that the use of the composition of DAFS-25+Polizon at a dose of 2 mg/kg increases the number of erythrocytes and hemoglobin in the experimental group and reduces the amount of white blood cells. The serum content of total protein, phosphorus and calcium increases in the group of experimental animals. Microscopic examination of the liver revealed no changes in the structure of the organ and hepatocytes in the experimental group, whereas in the control group hemodynamic disorders and dystrophic changes in liver cells were observed. Thus, the use of the composition DAFS-25+Polizon at a dose of 2 mg/kg of live weight in fattening bulls black-and-white breed contributes to the increase of redox processes in the body, stimulation of metabolism, prevent the development of liver disorders of cellular mechanisms of metabolism, optimizes the structure of the liver, which generally provides higher productivity.


Author(s):  
M. S. Bugaeva ◽  
O. I. Bondarev ◽  
N. N. Mikhailova ◽  
L. G. Gorokhova

Introduction. The impact on the body of such factors of the production environment as coal-rock dust and fluorine compounds leads to certain shift s in strict indicators of homeostasis at the system level. Maintaining the relative constancy of the internal environment of the body is provided by the functional consistency of all organs and systems, the leading of which is the liver. Organ repair plays a crucial role in restoring the structure of genetic material and maintaining normal cell viability. When this mechanism is damaged, the compensatory capabilities of the organ are disrupted, homeostasis is disrupted at the cellular and organizational levels, and the development of the main pathological processes is noted.The aim of the study is to compare the morphological mechanisms of maintaining structural homeostasis of the liver in the dynamics of the impact on the body of coal-rock dust and sodium fluoride.Materials and methods. Experimental studies were conducted on adult white male laboratory rats. Features of morphological mechanisms for maintaining structural homeostasis of the liver in the dynamics of exposure to coal-rock dust and sodium fluoride were studied on experimental models of pneumoconiosis and fluoride intoxication. For histological examination in experimental animals, liver sampling was performed after 1, 3, 6, 9, 12 weeks of the experiment.Results. The specificity of morphological changes in the liver depending on the harmful production factor was revealed. It is shown that chronic exposure to coal-rock dust and sodium fluoride is characterized by the development of similar morphological changes in the liver and its vessels from the predominance of the initial compensatory-adaptive to pronounced violations of the stromal and parenchymal components. Long-term inhalation of coal-rock dust at 1–3 weeks of seeding triggers adaptive mechanisms in the liver in the form of increased functional activity of cells, formation of double-core hepatocytes, activation of immunocompetent cells and endotheliocytes, ensuring the preservation of the parenchyma and the general morphostructure of the organ until the 12th week of the experiment. Exposure to sodium fluoride leads to early disruption of liver compensatory mechanisms and the development of dystrophic changes in the parenchyma with the formation of necrosis foci as early as the 6th week of the experiment.Conclusions. The study of mechanisms for compensating the liver structure in conditions of long-term exposure to coal-rock dust and sodium fluoride, as well as processes that indicate their failure, and the timing of their occurrence, is of theoretical and practical importance for developing recommendations for the timely prevention and correction of pathological conditions developing in employees of the aluminum and coal industry.The authors declare no conflict of interests.


Author(s):  
Elena A. Beigel ◽  
Natalya G. Kuptsova ◽  
Elena V. Katamanova ◽  
Oksana V. Ushakova ◽  
Oleg L. Lakhman

Introduction. Occupational chronic obstructive pulmonary disease (COPD) is one of the leading nosological forms of occupational respiratory disease. Numerous studies have shown high effectiveness of the combination of indacaterol/glycopyrronium (Ultibro®breezhaler®) on the impact on clinical and functional indicators in the treatment of COPD in General practice.The aim of the investigation the case of occupational COPD with the analysis of the dynamics of functional indicators, tolerance to physical load and evaluation of the quality of life of workers engaged in aluminum production by using combination of indacaterol/glycopyrronium.Materials and methods. The random sampling method included 20 men, workers of aluminum production, with the established diagnosis of professional COPD at the age of 40 to 60 years. The survey was conducted (Borg scale, medical Research Council scale (mMRC) and COPD Assessment Test (CAT). Functional methods of studies were conducted: spirometry, body plethysmography, electrocardiography (ECG) and the six-minute stepper test (6-MST).Results. Against the background of 8 weeks of therapy, the volume of forced exhalation for 1 minute (FEV1) increased by 14.7% and amounted to 67.90% of the due values, the forced vital capacity of the lungs (FVC) increased by 11.3% and amounted to 76.95% of the due. According to the body plethysmography (BPG) is set to decrease in residual lung volume on average by 13.4% and static hyperinflation, confirmed by the decrease in functional residual volume (FRV) of 18.8%. During the study period increased physical activity of patients. The average difference between the distance traveled in the six-minute step test before and after treatment was 58.8 m. The analysis of personal data showed that the quality of life of patients improved, the total score in the questionnaire CAT at the beginning of the study was 16.9 points, and after 8 weeks decreased by 63% and amounted to 10.7 points.Conclusions: The Results indicate a positive effect of combination therapy with indacaterol/glycopyrronium on the course and progression of occupational COPD.


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