scholarly journals Immunohistochemical analysis of ADAMTS-1, versican and pEGFR expressions in periapical granuloma and radicular cyst

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Nádia Marielly Gomes Batista ◽  
Antonia Taiane Lopes de Moraes ◽  
Karolyny Martins Balbinot ◽  
Osvaldo Rodrigues de Souza Neto ◽  
Juliana Melo da Silva Brandão ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Immunohistochemical Analysis ◽  
Dental Follicle ◽  
Radicular Cyst ◽  
Periapical Lesions ◽  
Periapical Granuloma ◽  
Inflammatory Processes

Abstract Background ADAMTS expression can be associated with several inflammatory processes, and has been correlated with tumorigenesis of some neoplasms, but its participation in the development of periapical lesions has not been investigated. Therefore, our objective was to verify the expression of ADAMTS-1, versican and pEGFR in Periapical Granuloma (PG) and in the Radicular Cyst (RC) since they are the most common lesions of the periapex. Methods 25 samples of RC and 10 of PG were used. As a control, 10 samples of inflammatory fibrous hyperplasia (IFH) and 10 of dental follicle (DF) were used. The expression of these proteins was investigated using immunohistochemistry. Results In the epithelium of RC, IFH and DF, the expression of ADAMTS-1 was greater in DF than in RC (p < .001). Versicano showed greater expression in IFH than in RC, DF than in RC (p < .001). pEGFR showed greater expression in IFH and RC than in DF (p < .01 and p < .05, respectively). In connective tissue, ADAMTS-1 expression was greater in PG and RC than in IFH and DF (p < .001). Versicano showed greater expression in PG, RC and IFH compared to DF (p < .001). In pEGFR there was a higher expression in PG when compared to RC, IFH and DF (p < .001). Greater immunostaining occurred in the RC than in the DF (p < .001). Conclusions Our results suggest that the studied proteins may participate in the pathogenesis of PG and RC, through the interaction of these proteins, in the remodeling of the ECM (versican) by ADAMTS-1, producing bioactive fragments, which could activate EGFR, contributing to the formation, growth and maintenance of injuries.

scholarly journals Comparative Metabolomics Reveals the Microenvironment of Common T-Helper Cells and Differential Immune Cells Linked to Unique Periapical Lesions

2021 ◽  
Vol 12 ◽  
Author(s):  
Alaa Muayad Altaie ◽  
Thenmozhi Venkatachalam ◽  
Lakshman P. Samaranayake ◽  
Sameh S. M. Soliman ◽  
Rifat Hamoudi
Keyword(s):  
Lactic Acid ◽  
Gas Chromatography Mass Spectrometry ◽  
Oral Lesions ◽  
Radicular Cyst ◽  
Pulp Tissue ◽  
Periapical Lesions ◽  
Periapical Abscess ◽  
Periapical Granuloma ◽  
Healthy Control ◽  
Active Genes

Periapical abscesses, radicular cysts, and periapical granulomas are the most frequently identified pathological lesions in the alveolar bone. While little is known about the initiation and progression of these conditions, the metabolic environment and the related immunological behaviors were examined for the first time to model the development of each pathological condition. Metabolites were extracted from each lesion and profiled using gas chromatography-mass spectrometry in comparison with healthy pulp tissue. The metabolites were clustered and linked to their related immune cell fractions. Clusters I and J in the periapical abscess upregulated the expression of MMP-9, IL-8, CYP4F3, and VEGF, while clusters L and M were related to lipophagy and apoptosis in radicular cyst, and cluster P in periapical granuloma, which contains L-(+)-lactic acid and ethylene glycol, was related to granuloma formation. Oleic acid, 17-octadecynoic acid, 1-nonadecene, and L-(+)-lactic acid were significantly the highest unique metabolites in healthy pulp tissue, periapical abscess, radicular cyst, and periapical granuloma, respectively. The correlated enriched metabolic pathways were identified, and the related active genes were predicted. Glutamatergic synapse (16–20),-hydroxyeicosatetraenoic acids, lipophagy, and retinoid X receptor coupled with vitamin D receptor were the most significantly enriched pathways in healthy control, abscess, cyst, and granuloma, respectively. Compared with the healthy control, significant upregulation in the gene expression of CYP4F3, VEGF, IL-8, TLR2 (P &lt; 0.0001), and MMP-9 (P &lt; 0.001) was found in the abscesses. While IL-12A was significantly upregulated in cysts (P &lt; 0.01), IL-17A represents the highest significantly upregulated gene in granulomas (P &lt; 0.0001). From the predicted active genes, CIBERSORT suggested the presence of natural killer cells, dendritic cells, pro-inflammatory M1 macrophages, and anti-inflammatory M2 macrophages in different proportions. In addition, the single nucleotide polymorphisms related to IL-10, IL-12A, and IL-17D genes were shown to be associated with periapical lesions and other oral lesions. Collectively, the unique metabolism and related immune response shape up an environment that initiates and maintains the existence and progression of these oral lesions, suggesting an important role in diagnosis and effective targeted therapy.

Immunohistochemical Study of Presence of T Cells, B Cells, and Macrophages in Periradicular Lesions of Primary Teeth

2008 ◽  
Vol 32 (4) ◽  
pp. 287-293 ◽  
Author(s):  
Michele Bolan ◽  
Daniele de Almeida Lima ◽  
Cláudia Pinto Figueiredo ◽  
Gabriella Di Giunta ◽  
Maria José de Carvalho Rocha
Keyword(s):  
T Cells ◽  
Immune System ◽  
B Cells ◽  
Primary Teeth ◽  
Microscopic Analysis ◽  
Inflammatory Cells ◽  
Periapical Lesions ◽  
Inflammatory Processes ◽  
Local Inflammatory Reaction

BACKGROUND: The periapical lesion is the result of a local inflammatory reaction caused by bacteria and its products present on the root canal. The interaction between inflammatory cells and bacteria elicit both specific and non-specific immune responses. OBJETIVE: Due to the lack of studies evaluating the role of the immune system in periapical lesions of primary teeth and considering the potentially systemic effects that these infections can cause in children, especially because of the immaturity of their immune system, we sought to evaluate the presence of T cells, B cells and macrophages on periradicular lesions in primary teeth. STUDY DESIGN: 14 periradicular lesions were analyzed. The immunohistochemistry technique was performed using CD45RO, CD20, CD68 monoclonal antibodies aiming to identify T cells, B cells and macrophages, respectively. Cells were quantified by microscopic analysis of histological sections. RESULTS: Mean percentage of positive cells CD45RO was 11.76; CD20 was 5.25; CD68 was 10.92. Our results showed that T and B cells and macrophages comprise the majority of the inflammatory infiltrate. CONCLUSION: We concluded that both humoral and cell mediated immune reactions take place in periradicular lesions of primary teeth. The immune system plays an important role on the periradicular inflammatory processes in primary teeth.

scholarly journals Method for the prevention of inflammatory processes in the bone part of the external auditory canal accompanied by the growth of connective tissue

2020 ◽  
Vol 19 (4) ◽  
pp. 21-26
Author(s):  
I. A. Anikin ◽  
◽  
S. A. Eremin ◽  
A. E. Shinkareva ◽  
◽  
...  
Keyword(s):  
Hearing Loss ◽  
Connective Tissue ◽  
Granulation Tissue ◽  
Satisfactory Result ◽  
External Auditory Canal ◽  
Inflammatory Process ◽  
Local Therapy ◽  
Scar Tissue ◽  
Inflammatory Processes ◽  
Bone Section

Otosurgery plays an important role in the rehabilitation of patients with hearing loss. Any operation can be accompanied by a long inflammatory process with excessive development of connective tissue, forming atresia or stenosis of the external auditory canal. Formed scars in the external auditory canal reduce the effectiveness of treatment and can lead to reoperation. In the treatment of scars, the use of injections of a suspension of prolonged steroids directly into the developing connective tissue is effective. We have developed a method of injecting drugs into the scar tissue of the bone section of the external auditory canal, which allows the use of such therapy in patients after otosurgery. 12 people were treated with the proposed technique. All patients had an inflammatory process in the external auditory canal with undesirable growth of connective tissue. Previous local therapy did not bring a satisfactory result. Patients received injections of a suspension of steroids into developing scar tissue. The treatment period was 1-8 weeks, for which 1 to 4 sessions of injections were performed. As a result of treatment according to the developed method, in all cases a cessation of the pathological inflammatory process was observed. Timely therapy made it possible to completely eliminate the excess granulation tissue (100%), to stop the growth of the scar, or even to cause a significant decrease (33%). The developed method allows to use of a small dose of a substance (0,1–0,5 ml) for injection to achieve the desired therapeutic effect.

scholarly journals Comparative evaluation to determine the efficacy of conventional radiography, digital radiography and ultrasound imaging in the diagnosis of periapical lesions

2021 ◽  
Vol 1 (3) ◽  
Author(s):  
Rajesh Gupta ◽  
Preety Gupta ◽  
Shivani Gupta, ◽  
Sumit Garg
Keyword(s):  
Ultrasound Imaging ◽  
Digital Radiography ◽  
Histopathological Examination ◽  
Conventional Radiography ◽  
Test Results ◽  
Periapical Lesions ◽  
Periapical Granuloma ◽  
Mandibular Teeth ◽  
Wallis Anova ◽  
Histopathological Investigation

In recent time several imaging modalities have been implicated for diagnosing periapical lesion. The present study is carried out to determine and compare the nature and extent of periapical lesions using conventional radiography, digital radiography and ultrasound imaging and to confirm the diagnosis with histopathological examination. Thirty patients aged between 15 and 45 years with well-defined periapical radiolucency associated with anterior maxillary or mandibular teeth requiring endodontic surgery or extraction were selected. The pre-operative intraoral periapical radiographs and digital images and were diagnosed by three observers. Ultrasound examination was performed and the images were assessed by an ultrasonographer. The periapical tissues were obtained to enable histopathological investigation. The data was statistically analysed using the kappa procedure, Kruskal Wallis Anova & Mann-Whitney U test. Results showed that the percentage accuracy of diagnosing periapical lesions using conventional radiography, digital radiography and ultrasound was – 86.67%, 93.33% & 93.33% respectively. Thus, we conclude that ultrasound helps in distinguishing between periapical granuloma and cyst which is of importance in predicting the treatment outcome. So, ultrasound can be used as an adjunct to conventional or digital radiography in diagnosing periapical lesions.

scholarly journals Peculiarities of endodontic treatment of chronic symptomatic periodontitis on oncological disease and chemotherapy

2019 ◽  
Vol 06-07 (1-2) ◽  
pp. 8-11
Author(s):  
O.V. Vozniy ◽  
A.M. Filon
Keyword(s):  
Oral Cavity ◽  
Dental Care ◽  
Endodontic Treatment ◽  
Malignant Neoplasms ◽  
Dental Disease ◽  
Chemotherapy Treatment ◽  
Periapical Lesions ◽  
High Prevalence ◽  
Inflammatory Processes ◽  
Methods Of Treatment

Providing dental care to cancer patients has been and remains very relevant. Studies of the oral cavity in this category of patients have found a high prevalence of dental disease and, as a consequence, a significant need for dental care. Chemotherapy can cause complications such as stomatitis, mucositis, exacerbation of chronic pulpitis, and periodontitis. Patients with an unsanctioned oral cavity run the risk of developing these complications, unlike patients who regularly visit the dentist. The aim of our work was to study the features of endodontic treatment of patients with malignant neoplasms receiving chemotherapy treatment and to confirm the use of atraumatic and conservative methods of treatment of periapical inflammatory processes. The results obtained confirm that the elimination of infection in the root canal of the teeth leads to the regeneration of periapical lesions of bone tissue. The size of the lesion does not affect the tactics of treatment.

scholarly journals A Quiescent Colossal Radicular Cyst: A Diverse Histological Presentation

2013 ◽  
Vol 4 (4) ◽  
pp. 286-290
Author(s):  
V Reshma ◽  
Radhika M Bavle ◽  
BK Varsha ◽  
BM Kavya
Keyword(s):  
Connective Tissue ◽  
Periodontal Ligament ◽  
Radicular Cyst ◽  
Cell Infiltrate ◽  
Jaw Cysts ◽  
Connective Tissue Capsule ◽  
Tissue Capsule

ABSTRACT Radicular cysts are the most frequent inflammatory cysts comprising of about 52 to 68% of all the jaw cysts. It arises from the epithelial residues in the periodontal ligament space following pulpal necrosis. The distinguished lining of the cystic lumen is hyperplastic stratified squamous nonkeratinizing epithelium showing arcading pattern with dense infammatory cell infiltrate, cholesterol clefts, Russell bodies and Ruston bodies in the connective tissue capsule. Here, we present an unusual large radicular cyst lined almost entirely by quiescent atrophic epithelium, which is innocuous, persistent, has enlarged overtime, with minimal infammatory cells in the connective tissue capsule. How to cite this article Reshma V, Bavle RM, Varsha BK, Kavya BM. A Quiescent Colossal Radicular Cyst: A Diver Histological Presentation. World J Dent 2013;4(4):286-290.

scholarly journals The Distribution of Abcc6 in Normal Mouse Tissues Suggests Multiple Functions for this ABC Transporter

2003 ◽  
Vol 51 (7) ◽  
pp. 887-902 ◽  
Author(s):  
Konstanze Beck ◽  
Kimiko Hayashi ◽  
Brian Nishiguchi ◽  
Olivier Le Saux ◽  
Masando Hayashi ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Epithelial Cells ◽  
Connective Tissue ◽  
Tissue Distribution ◽  
Cellular Localization ◽  
Immunohistochemical Analysis ◽  
Pseudoxanthoma Elasticum ◽  
Rnase Protection ◽  
Mouse Tissues ◽  
Extracellular Matrix Components

We have studied the tissue distribution of Abcc6, a member of the ABC transmembrane transporter subfamily C, in normal C57BL/6 mice. RNase protection assays revealed that although almost all tissues studied contained detectable levels of the mRNA encoding Abcc6, the highest levels of Abcc6 mRNA were found in the liver. In situ hybridization (ISH) demonstrated abundant Abcc6 mRNA in epithelial cells from a variety of tissues, including hepatic parenchymal cells, bile duct epithelia, kidney proximal tubules, mucosa and gland cells of the stomach, intestine, and colon, squamous epithelium of the tongue, corneal epithelium of the eye, keratinocytes of the skin, and tracheal and bronchial epithelium. Furthermore, we detected Abcc6 mRNA in arterial endothelial cells, smooth muscle cells of the aorta and myocardium, in circulating leukocytes, lymphocytes in the thymus and lymph nodes, and in neurons of the brain, spinal cord, and the specialized neurons of the retina. Immunohistochemical analysis using a polyclonal Abcc6 rabbit antibody confirmed the tissue distribution of Abcc6 suggested by our ISH studies and revealed the cellular localization of Abcc6 in the basolateral plasma membrane in the epithelial cells of proximal convoluted tubules in the kidney. Although the function of Abcc6 is unknown, mutations in the human ABCC6 gene result in a heritable disorder of connective tissue called pseudoxanthoma elasticum (PXE). Our results demonstrating the presence of Abcc6 in epithelial and endothelial cells in a variety of tissues, including those tissues affected in PXE patients, suggest a possible role for Abcc6 in the normal assembly of extracellular matrix components. However, the presence of Abcc6 in neurons and leukocytes, two cell populations not associated with connective tissue, also suggests a more complex multifunctional role for Abcc6.

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