The expression levels of MicroRNA-146a, RANKL and OPG after non-surgical periodontal treatment

Abstract Objective MicroRNA-146a (miR-146a) is a regulator of inflammatory response. Periodontitis is a disease with immune pathophysiology of the periodontium in which the inflammation results in the destruction of the soft tissues and alveolar bone. Therefore, the aim of this study was to investigate the expressions of miR-146a, OPG, and RANKL in diseased and healthy periodontal tissues to understand whether miR-146a expression level may associate with OPG and RANKL mRNA levels and OPG/RANKL ratio after non-surgical periodontal treatment. Methods The levels of miR-146a, RANKL, and OPG in gingival tissues from patients with generalized periodontitis stages II and III and grades A and B (n = 15, group A), patients with generalized periodontitis stages III and IV and grade C (n = 15, group B), and healthy individuals (n = 10) were determined by real-time PCR. The associations of miR-146a expression with OPG and RANKL levels were evaluated. Results The levels of miR-146a in two subgroups within periodontitis patients were significantly higher than healthy subjects (P < 0.0001). MiR-146a showed the increased level in group A of patients compared with group B (P < 0.05). Clinical parameters such as probing depth (PD) and clinical attachment loss (CAL) were significantly higher in patients than control group (P < 0.05). The levels of OPG and RANKL were increased in patients compared with healthy subjects, although the elevated levels were not statistically significant. MiR-146a was not associated with OPG and RANKL levels and OPG/RANKL ratio. Conclusions The results of this study failed to show the associations of miR-146a level with OPG and RANKL levels and OPG/RANKL ratio in periodontitis after non-surgical periodontal treatment.

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Micro RNA-146a effect on Periodontitis is Independent on OPG/RANKL Axis

10.21203/rs.3.rs-598474/v1 ◽

2021 ◽

Author(s):

Mandana Sattari ◽

Somayeh Ghotloo ◽

Ramezan-Ali Taheri ◽

Reza ArefNezhad ◽

Hossein Motedayyen

Keyword(s):

Healthy Subjects ◽

Soft Tissues ◽

Alveolar Bone ◽

Micro Rna ◽

Control Group ◽

Clinical Attachment Loss ◽

Probing Depth ◽

Independent Mechanism ◽

Group A ◽

Group B

Abstract Background MicroRNA-146a (miR-146a) is a regulator of inflammatory response. Periodontitis is a disease with immune pathophysiology of the periodontium in which the inflammation results in the destruction of the soft tissues and alveolar bone. Therefore, this study was investigated whether miR-146a may contribute to periodontitis through affecting the levels of OPG and RANKL. Methods The levels of miR-146a, RANKL, and OPG in gingival tissues from patients with generalized periodontitis stages II and III and grades A and B (n = 15, group A), patients with generalized periodontitis stages III and IV and grade C (n = 15, group B), and healthy individuals (n = 10) were determined by real-time PCR. The associations of miR-146a expression with OPG and RANKL levels were evaluated. Results The levels of miR-146a in two subgroups within periodontitis patients were significantly higher than healthy subjects (P < 0.0001). MiR-146a showed the increased level in group A of patients compared with group B (P < 0.05). Clinical parameters such as probing depth (PD) and clinical attachment loss (CAL) were significantly higher in patients than control group (P < 0.05). The levels of OPG and RANKL were increased in patients compared with healthy subjects, although the elevated levels were not statistically significant. MiR-146a was not associated with the levels of OPG and RANKL. Conclusion The results of this study failed to show the effect of miR-146a on the pathophysiology of disease through OPG/RANKL axis. Clinical Relevancy: MiR-146a may participate in the pathophysiology of disease through independent mechanism(s) of OPG/RANKL axis.

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Efek Seduhan Kopi Robusta terhadap Jumlah Limfosit pada Daerah Tarikan Ligamen Periodontal Gigi Marmut (Cavia Cobaya) yang Diinduksi Gaya Mekanis Ortodonti

Pustaka Kesehatan ◽

10.19184/pk.v7i3.11560 ◽

2020 ◽

Vol 7 (3) ◽

pp. 182

Author(s):

Ginanjar Hidayatullah ◽

Herniyati Herniyati ◽

Leliana Sandra Deviade Putri

Keyword(s):

Periodontal Ligament ◽

Alveolar Bone ◽

Control Group ◽

Control Group Design ◽

Robusta Coffee ◽

Coffee Brew ◽

Significant Difference ◽

Group A ◽

Group B ◽

Coffea Robusta

Malocclusion is an occlusion that deviates from the normal state, so it needs to be treated using an orthodontic device. The formation of new alveolar bone after obtaining an orthodontic force will be surrounded by osteoid bones are not stable enough (relapse). Robusta coffee (Coffea robusta) has flavonoids which can increase lymphocyte proliferation. The purpose of this study was to analyze the effect of robusta coffee on the number of lymphocyte cells in the tension area of marmot’s (Cavia cobaya) periodontal ligament which induced by mechanical orthodontic forces. This type of research is laboratory experimental with the Post Test Only Control Group Design. The average number of lymphocyte cells in group A = 0.75, B = 1.45, C = 1.26, D = 1.54. One-way Anova test results showed that the number of lymphocyte cells in all groups showed a significant difference (p <0.05). The results of the post hoc LSD test showed that group A had significant differences with group B and group D (p <0.05). While group C did not have a significant difference with group A (p> 0.05). The conclusion obtained is that the provision of robusta coffee brew for 2 weeks can increase the number of lymphocyte cells in the tension area of marmot’s (Cavia cobaya) periodontal ligament which induced by orthodontic mechanical forces compared to without robusta coffee brew.

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Effects of Corticision on Paradental Remodeling in Orthodontic Tooth Movement

The Angle Orthodontist ◽

10.2319/020308-60.1 ◽

2009 ◽

Vol 79 (2) ◽

pp. 284-291 ◽

Cited By ~ 96

Author(s):

Su-Jung Kim ◽

Young-Guk Park ◽

Seung-Goo Kang

Keyword(s):

Alveolar Bone ◽

Tooth Movement ◽

Orthodontic Tooth Movement ◽

Control Group ◽

Orthodontic Force ◽

Alveolar Bone Remodeling ◽

Biologic Effects ◽

Group A ◽

Group B ◽

Tissue Specimens

Abstract Objective: To investigate the biologic effects of Corticision on alveolar remodeling in orthodontic tooth movement. Materials and Methods: In this study, 16 cats were divided into 3 groups: group A, only orthodontic force (control); group B, orthodontic force plus Corticision; and group C, orthodontic force plus Corticision and periodic mobilization. Histologic and histomorphometric studies were performed on tissue specimens on days 7, 14, 21, and 28. Results: Extensive direct resorption of bundle bone with less hyalinization and more rapid removal of hyalinized tissue were observed in group B. The accumulated mean apposition area of new bone on day 28 was observed to be 3.5-fold higher in group B than in the control group A. Conclusions: Corticision might be an efficient procedure for accelerating orthodontic tooth movement accompanied with alveolar bone remodeling.

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The Gaseous Ozone Therapy as a Promising Antiseptic Adjuvant of Periodontal Treatment: A Randomized Controlled Clinical Trial

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph19020985 ◽

2022 ◽

Vol 19 (2) ◽

pp. 985

Author(s):

Biagio Rapone ◽

Elisabetta Ferrara ◽

Luigi Santacroce ◽

Skender Topi ◽

Antonio Gnoni ◽

...

Keyword(s):

Therapy Group ◽

Randomized Study ◽

Periodontal Treatment ◽

Controlled Clinical Trial ◽

Ozone Therapy ◽

Periodontal Tissues ◽

Gaseous Ozone ◽

Significant Difference ◽

Group A ◽

Group B

Background: the establishment of periodontitis is regulated by the primary etiological factor and several individual conditions including the immune response mechanism of the host and individual genetic factors. It results when the oral homeostasis is interrupted, and biological reactions favor the development and progression of periodontal tissues damage. Different strategies have been explored for reinforcing the therapeutic effect of non-surgical periodontal treatment of periodontal tissue damage. Gaseous ozone therapy has been recognized as a promising antiseptic adjuvant, because of its immunostimulating, antimicrobial, antihypoxic, and biosynthetic effects. Then, we hypothesized that the adjunct of gaseous ozone therapy to standard periodontal treatment may be leveraged to promote the tissue healing response. Methods: to test this hypothesis, we conducted a prospective randomized study comparing non-surgical periodontal treatment plus gaseous ozone therapy to standard therapy. A total of 90 healthy individuals with moderate or severe generalized periodontitis were involved in the study. The trial was conducted from September 2019 to October 2020. Forty-five patients were randomized to receive scaling and root-planning (SRP) used as conventional non-surgical periodontal therapy plus gaseous ozone therapy (GROUP A); forty-five were allocated to standard treatment (GROUP B). The endpoint was defined as the periodontal response rate after the application of the ozone therapy at 3 months and 6 months, defined as no longer meeting the criteria for active periodontitis. Statistical analysis was performed employing SPSS v.18 Chicago: SPSS Inc. Results: periodontal parameters differed significantly between patients treated with the two distinct procedures at 3 months (p ≤ 0.005); a statistically significant difference between groups was observed from baseline in the CAL (p ≤ 0.0001), PPD (p ≤ 0.0001) and BOP (p ≤ 0.0001) scores. Conclusions: The present study suggests that SRP combined with ozone therapy in the treatment of periodontitis revealed an improved outcome than SRP alone.

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Lesion Sterilization Tissue Repair as Adjunct to Conventional Root Canal Treatment of Combined Periodontic-Endodontic Cases

World Journal of Dentistry ◽

10.5005/jp-journals-10015-1257 ◽

2014 ◽

Vol 5 (1) ◽

pp. 47-52

Author(s):

Ahmed A Saleh ◽

Hossam A Eid ◽

Khalid M Abdelaziz

Keyword(s):

Root Canal ◽

Tissue Repair ◽

Periodontal Treatment ◽

Root Canal Treatment ◽

Control Group ◽

Group A ◽

Group B ◽

Rate Of Failure ◽

Periodontal Probing

ABSTRACT Background Treatment of perio-endo lesions is challenging for the clinician. A combination of root canal treatment and periodontal treatment is needed in order to provide good results. There is a high failure rate due to the difficulty in eliminating the microflora that colonizes and complicates the lesion. Conventional root canal treatment (RCT) sometimes is not enough to prevent failure in such lesions. Hence the Aim of this study was to evaluate of use of LSTR (Lesion Sterilization Tisssue Repair) therapy in conjunction with, periodontal therapy and RCT in management of combined perio-endo lesions. Materials and methods This study was conducted on 26 patients with perio-endo lesions, The affected teeth were tender to percussion, presented no mobility, and meticulous periodontal probing revealed all pockets to be more than 6 mm in depth. Periapical radiographs of the teeth confirmed the extensive coronal carious lesion involving the pulp and revealed large periapical lesion. The patients were classified into two groups, each of 14 patients, control group (A) RCT done by using conventional sealer, and group (B), RCT done by mixing sealer with LSTR material in equal proportions. All teeth were treated by open flap debridement. Results In group B patients, after follow-up for 3, 6, 9 and 12 months, we found excellent prognosis (98%), with no symptoms of pain and there was marked improvement in periodontal parameters for all teeth. However, on the other hand, in group A patients, the prognosis was found to be 90%. Conclusion According to results of this study, we highly recommend the use of LSTR with sealer in RCT together with periodontal treatment in management of perio-endo lesions to decrease the rate of failure and improve the periodontal condition. How to cite this article Saleh AA, Eid HA, Abdelaziz KM. Lesion Sterilization Tissue Repair as Adjunct to Conventional Root Canal Treatment of Combined Periodontic-Endodontic Cases. World J Dent 2014;5(1):47-52.

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Evaluation of Salmonella Gallinarum ghost formulated with Montanide™ ISA 70 VG adjuvant as a vaccine against fowl typhoid

Acta Veterinaria Hungarica ◽

10.1556/004.2015.038 ◽

2015 ◽

Vol 63 (4) ◽

pp. 401-412 ◽

Cited By ~ 5

Author(s):

Chetan V. Jawale ◽

Nithiphonh Somsanith ◽

Seong Kug Eo ◽

Sang-Youel Park ◽

John Hwa Lee

Keyword(s):

Virulent Strain ◽

Survival Rates ◽

Control Group ◽

Mrna Levels ◽

Salmonella Gallinarum ◽

B Subunit ◽

Fowl Typhoid ◽

Group A ◽

Group B ◽

Th1 Cytokines

Escherichia coli heat-labile enterotoxin B subunit (LTB) protein is a potent adjuvant. Salmonella Gallinarum ghosts carrying LTB (S. Gallinarum-LTB ghosts) were genetically constructed using a plasmid, pJHL187-LTB, designed for the co-expression of the LTB and E lysis proteins. This study evaluates the immunopotentiating effects of Montanide™ ISA 70 VG on S. Gallinarum-LTB ghost vaccination against fowl typhoid. Five-week-old layer chickens were injected intramuscularly with sterile PBS (non-immunised control, Group A), S. Gallinarum-LTB ghost (Group B) or S. Gallinarum-LTB ghost emulsified with Montanide™ ISA 70 VG adjuvant (Group C). Chickens from both Groups B and C showed significant induction of antigen-specific systemic IgG response compared to controls; in addition, Group C showed enhanced induction of systemic IgG response compared to Group B. We observed significant induction of antigen-specific lymphocyte proliferative response and increased mRNA levels of Th1 cytokines (IFN-γ and IL2) in both Groups B and C. Furthermore, in the challenge experiment with a virulent strain of S. Gallinarum, Group C showed higher survival rates compared with other groups. These results indicate that vaccination with the S. Gallinarum-LTB ghost in combination with Montanide™ ISA 70 VG may enhance the protective immunity against fowl typhoid.

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Clinical effect of Suvarna Bindu Prashan

Journal of Ayurveda and Integrated Medical Sciences (JAIMS) ◽

10.21760/jaims.v2i3.8203 ◽

2017 ◽

Vol 2 (3) ◽

Cited By ~ 1

Author(s):

Patil Aniket ◽

Dindore Pallavi ◽

Arbar Aziz ◽

Kadam Avinash ◽

Saroch Vikas

Keyword(s):

Physical Health ◽

Eating Habits ◽

Control Group ◽

Inform Consent ◽

Significant Difference ◽

Group A ◽

Group B ◽

Immune Booster ◽

Mental And Physical Health ◽

Apparently Healthy

The quest for excellence in mental and physical health is not new. We find various references and formulations in Ayurvedic classics meant for promoting mental and physical health of a child. Suvarna Prashan is one of the formulations explained in age old Ayurvedic classic Kashyap Samhita. This formulation is very widely used now days as a memory and immune booster for children. But there is very little systematic documented study which can be used to evaluate the efficacy of the formulation. Suvarna Bhasma was prepared in Ayurved Rasayani Pharmacy, Pune. Madhu and Ghrita were collected from KLE Ayurveda Pharmacy, Belgaum. Suvarna Bindu Prashan was prepared in KLE Ayurved pharmacy, Belgaum. It contains Suvarna Bhasma, Ghrita and Madhu. Twenty apparently healthy male and female children with age group of three to four years were ready to sign inform consent form were selected into two groups each. Subjects in Group A received Suvarna Bindu Prashan where as Group B (Control group) did not receive any treatment. Both the groups were observed for six months. Children in Suvarna Bindu Prashan group showed significant reduction in the scores of eating habits, behavior, mood, temperament and scores of event of illness. However there was no significant difference in the score of sleeping habit. There was significant increase in IQ percentage.

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Microecological preparation combined with an modified low-carbon diet improves glucolipid metabolism and cardiovascular complication in obese patients

Diabetology & Metabolic Syndrome ◽

10.1186/s13098-021-00697-6 ◽

2021 ◽

Vol 13 (1) ◽

Author(s):

Jianguo Liu ◽

Liehui Xiao ◽

Hezhongrong Nie ◽

Yong Pan ◽

Yan Liu ◽

...

Keyword(s):

Intestinal Barrier ◽

Intestinal Barrier Function ◽

Control Group ◽

Obese Patients ◽

Carotid Atherosclerotic Plaque ◽

Low Carbon ◽

Balanced Diet ◽

Group A ◽

Group B ◽

Control Group Group

Abstract Objective To investigate the impact of microecological preparation combined with modified low-carbon diet on the glucolipid metabolism and cardiovascular complication in obese patients. Methods From August 2017 to July 2020, 66 obese patients were recruited, and administrated with an modified low-carbon diet with (group A) or without (Group B) microecology preparation and a balanced diet in control group (group C) for 6 months. Meanwhile, 20 volunteers administrated with a balanced diet were recruited as the healthy control group (group D). Results After 6-month intervention, obese subjects in group A and B showed significant improvement of body and liver fat mass, reduction of serum lipid levels, intestinal barrier function markers, insulin resistance index (IRI), high blood pressure (HBP) and carotid intima thickness, as compared with subjects in group C. More importantly, subjects in group A had better improvement of vascular endothelial elasticity and intimal thickness than subjects in group B. However, these intervention had no effect on carotid atherosclerotic plaque. Conclusion Administration of microecological preparation combined with modified low-carbon diet had better improvement of intestinal barrier function, glucose and lipid metabolism, and cardiovascular complications than low-carbon diet in obese patients, but the effect of a simple low-carb diet on carotid atherosclerotic plaque need to be further addressed.

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Effects of different thermal insulation methods on the nasopharyngeal temperature in patients undergoing laparoscopic hysterectomy: a prospective randomized controlled trial

BMC Anesthesiology ◽

10.1186/s12871-021-01324-7 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Guanyu Yang ◽

Zefei Zhu ◽

Hongyu Zheng ◽

Shifeng He ◽

Wanyue Zhang ◽

...

Keyword(s):

Thermal Insulation ◽

Primary Outcome ◽

Controlled Trial ◽

Laparoscopic Hysterectomy ◽

Control Group ◽

Prospective Randomized Controlled Trial ◽

Warming Blanket ◽

Group A ◽

Insulation Effect ◽

Group B

Abstract Background This study explored the comparison of the thermal insulation effect of incubator to infusion thermometer in laparoscopic hysterectomy. Methods We assigned 75 patients enrolled in the study randomly to three groups: Group A: Used warming blanket; group B: Used warming blanket and infusion thermometer; group C: Used warming blanket and incubator. The nasopharyngeal temperature at different time points during the operation served as the primary outcome. Results The nasopharyngeal temperature of the infusion heating group was significantly higher than that of the incubator group 60 min from the beginning of surgery (T3): 36.10 ± 0.20 vs 35.81 ± 0.20 (P<0.001)90 min from the beginning of surgery (T4): 36.35 ± 0.20 vs 35.85 ± 0.17 (P<0.001). Besides, the nasopharyngeal temperature of the incubator group was significantly higher compared to that of the control group 60 min from the beginning of surgery (T3): 35.81 ± 0.20 vs 35.62 ± 0.18 (P<0.001); 90 min from the beginning of surgery (T4): 35.85 ± 0.17 vs 35.60 ± 0.17 (P<0.001). Regarding the wake-up time, that of the control group was significantly higher compared to the infusion heating group: 24 ± 4 vs 21 ± 4 (P = 0.004) and the incubator group: 24 ± 4 vs 22 ± 4 (P = 0.035). Conclusion Warming blanket (38 °C) combined infusion thermometer (37 °C) provides better perioperative thermal insulation. Hospitals without an infusion thermometer can opt for an incubator as a substitute. Trial registration This trial was registered with ChiCTR2000039162, 20 October 2020.

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The Bone Buttress Theory: The Effect of the Mechanical Loading of Bone on the Osseointegration of Dental Implants

Biology ◽

10.3390/biology10010012 ◽

2020 ◽

Vol 10 (1) ◽

pp. 12

Author(s):

David Chavarri-Prado ◽

Aritza Brizuela-Velasco ◽

Ángel Álvarez-Arenal ◽

Markel Dieguez-Pereira ◽

Esteban Pérez-Pevida ◽

...

Keyword(s):

Dental Implants ◽

Mechanical Loading ◽

Bone Growth ◽

Bone Matrix ◽

Test Group ◽

Control Group ◽

Group A ◽

Bone To Implant Contact ◽

The Stability ◽

Group B

Objectives: To determine the effect of mechanical loading of bone on the stability and histomorphometric variables of the osseointegration of dental implants using an experimental test in an animal model. Materials and Methods: A total of 4 human implants were placed in both tibiae of 10 New Zealand rabbits (n = 40). A 6-week osseointegration was considered, and the rabbits were randomly assigned to two groups: Group A (Test group) included 5 rabbits that ran on a treadmill for 20 min daily during the osseointegration period; Group B (Controls) included the other 5 that were housed conventionally. The monitored variables were related to the primary and secondary stability of the dental implants (implant stability quotient—ISQ), vertical bone growth, bone to implant contact (BIC), area of regenerated bone and the percentage of immature matrix. Results: The results of the study show a greater vertical bone growth (Group A 1.26 ± 0.48 mm, Group B 0.32 ± 0.47 mm, p < 0.001), higher ISQ values (Group A 11.25 ± 6.10 ISQ, 15.73%; Group B 5.80 ± 5.97 ISQ, 7.99%, p = 0.006) and a higher BIC (Group A 19.37%, Group B 23.60%, p = 0.0058) for implants in the test group, with statistically significant differences. A higher percentage of immature bone matrix was observed for implants in the control group (20.68 ± 9.53) than those in the test group (15.38 ± 8.84) (p = 0.108). A larger area of regenerated bone was also observed for the test implants (Group A 280.50 ± 125.40 mm2, Group B 228.00 ± 141.40 mm2), but it was not statistically significant (p = 0.121). Conclusions: The mechanical loading of bone improves the stability and the histomorphometric variables of the osseointegration of dental implants.

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