scholarly journals In vitro anti-allergic activity of Moringa oleifera Lam. extracts and their isolated compounds

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Nur Zahirah Abd Rani ◽  
Endang Kumolosasi ◽  
Malina Jasamai ◽  
Jamia Azdina Jamal ◽  
Kok Wai Lam ◽  
...  
Keyword(s):  
Histamine Release ◽  
Moringa Oleifera ◽  
Late Phase ◽  
Positive Control ◽  
Chromatographic Technique ◽  
Allergic Reactions ◽  
Ketotifen Fumarate ◽  
Tnf Α ◽  
New Compounds

Abstract Background Moringa oleifera Lam. is a commonly used plant in herbal medicine and has various reported bioactivities such as antioxidant, antimicrobial, anticancer and antidiabetes. It is rich in nutrients and polyphenols. The plant also has been traditionally used for alleviating allergic conditions. This study was aimed to examine the anti-allergic activity of M. oleifera extracts and its isolated compounds. Method M. oleifera leaves, seeds and pods were extracted with 80% of ethanol. Individual compounds were isolated using a column chromatographic technique and elucidated based on the nuclear magnetic resonance (NMR) and electrospray ionisation mass spectrometry (ESIMS) spectral data. The anti-allergic activity of the extracts, isolated compounds and ketotifen fumarate as a positive control was evaluated using rat basophilic leukaemia (RBL-2H3) cells for early and late phases of allergic reactions. The early phase was determined based on the inhibition of beta-hexosaminidase and histamine release; while the late phase was based on the inhibition of interleukin (IL-4) and tumour necrosis factor (TNF-α) release. Results Two new compounds; ethyl-(E)–undec-6-enoate (1) and 3,5,6-trihydroxy-2-(2,3,4,5,6-pentahydroxyphenyl)-4H-chromen-4-one (2) together with six known compounds; quercetin (3), kaempferol (4), β-sitosterol-3-O-glucoside (5), oleic acid (6), glucomoringin (7), 2,3,4-trihydroxybenzaldehyde (8) and stigmasterol (9) were isolated from M. oleifera extracts. All extracts and the isolated compounds inhibited mast cell degranulation by inhibiting beta-hexosaminidase and histamine release, as well as the release of IL-4 and TNF-α at varying levels compared with ketotifen fumarate. Conclusion The study suggested that M. oleifera and its isolated compounds potentially have an anti-allergic activity by inhibiting both early and late phases of allergic reactions.

scholarly journals Mediators of Anaphylaxis, Anaphylactoid Reactions, and Rhinitis

1987 ◽  
Vol 1 (1) ◽  
pp. 17-26 ◽  
Author(s):  
Kenneth P. Mathews
Keyword(s):  
Mast Cells ◽  
Allergic Reaction ◽  
Late Effects ◽  
Late Phase ◽  
Allergic Reactions ◽  
Cellular Interactions ◽  
Nasal Symptoms ◽  
Anaphylactoid Reactions

Besides histamine, a large and increasing number of mediators of allergic reactions are being found to be released by mast cells or basophils during anaphylactic reactions. Many of these same substances are released by stimuli other than allergen-IgE interactions, and this type of phenomenon (anaphylactoid or pseudo-allergic reaction) may account for some nasal symptoms that simulate allergy. In addition to rapidly developing reactions of these types, numerous recent investigations have emphasized the importance of late-phase reactions that occur as a consequence of the immediate reactivity. Besides mast cells and/or basophils, these late effects seem to involve a complex network of cellular interactions, which may include neutrophils, eosinophils, lymphocytes, macrophages, and platelets. Studies of nasal washings following allergen challenges in humans have provided cogent in vivo support of earlier hypotheses about mediator release based on in vitro experimentation.

scholarly journals COMPARISON OF α –GLUCOSIDASE INHIBITORY ACTIVITY OF Moringa oleifera ETHANOLIC EXTRACT

2021 ◽  
Vol 9 (Spl-2-ICOPMES_2020) ◽  
pp. S269-S273
Author(s):  
Rizky Rahmwaty Alami ◽  
◽  
Herlina Rante ◽  
Yulia Yusrini Djabir ◽  
◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Moringa Oleifera ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Glucosidase Inhibitor ◽  
Plant Samples

The purpose of this research was to determine the α-glucosidase enzyme inhibitory activity of Moringa oleifera plant samples collected from the three geographical areas viz., Saragi, Bacuhau, and Batumatongka of Southeast Sulawesi Indonesia. Ethanol extract of Moringa leaves was prepared by the maceration method using 95% ethanol. The estimation of α –glucosidase inhibitory activity of this extract was performed in vitro. The results of the study showed that ethanolic extract of three Moringa samples i.e. Sarangi, Bacuhau, and Batumatongka had the IC50value of 18.62, 10.18, 10.58 ppm, respectively while IC50value for the acarbose positive control was reported 11.54ppm. From the results of this study, it can be concluded that ethanolic extract of Moringa could inhibit α –glucosidase and this potential was similar to the commercial α –glucosidase inhibitor acarbose.

Chemical and biological activity of: Calea sp. from Panama; Centaurea spp. from Algeria; Hypericum spp. from Crete; Euphorbia spp. from Central Greece

2021 ◽  
Author(s):  
Μαρία-Ελένη Γραφάκου
Keyword(s):  
Biological Activity ◽  
National Cancer Institute ◽  
Ex Vivo ◽  
Positive Control ◽  
X Ray ◽  
Central Greece ◽  
Tnf Α

Τα φάρμακα φυσικής προέλευσης παίζουν καίριο ρόλο τόσο στην παραδοσιακή όσο και στην σύγχρονη ιατρική, καθώς η φύση παρέχει μια ανεξάντλητη πηγή βιοδραστικών συστατικών, και ο ρόλος των φυσικών προϊόντων στην εύρεση καινούργιων μορίων-οδηγών για την ανακάλυψη αποτελεσματικών φαρμάκων για μία πληθώρα ασθενειών καθημερινώς ενισχύεται. Με βάση σύγχρονα βιβλιογραφικά δεδομένα, η μελέτη είχε ως στόχο την αναζήτηση και τον εντοπισμό σεσκιτερπενικών λακτονών (από τα ενδημικά είδη Centaurea και Calea), φαινολικών παραγώγων και αιθερίων ελαίων (από ενδημικά είδη Hypericum), καθώς και διτερπενίων (από ενδημική Euphorbia), με έμφαση στην αντιφλεγμονώδη και κυτταροτοξική δράση. Αναφορικά με τα τέσσερα υπό εξέταση είδη, αν και διαφέρουν τόσο στην βοτανική κατάταξη, όσο και στο χημικό προφίλ, εμφανίζουν ομοιότητες, που αφορούν στη βιολογική δράση. Το Κενταύριο, το Υπερικό και η Ευφορβία αναφέρονται απο τον Ιπποκράτη (5ος αιώνας π.Χ.) και παρατηρούμε επίσης ότι έχουν καταγραφεί κοινές χρήσεις και για τα τέσσερα γένη στην παραδοσιακή λαϊκή θεραπευτική μέχρι σήμερα, και συγκεκριμένα αναφέρονται ως επουλωτικά, αντιπυρετικά, αντιφλεγμονώδη, διουρητικά, εμμηναγωγά, για γαστρεντερικές διαταραχές. Είναι σημαντικό ότι τα ενδημικά είδη με στενή εξάπλωση αποτελούν δυνητικά σημαντικές πηγές νέων βιοδραστικών δευτερογενών μεταβολιτών, δεδομένου ότι τα αντίστοιχα φυτικά είδη από τα ίδια γένη ευρείας όμως εξάπλωσης εμφανίζουν πληθώρα εθνοφαρμακολογικών αναφορών.Συνολικά 84 δευτερογενείς μεταβολίτες απομονώθηκαν από ενδημικά φυτά που φύονται ανά τον κόσμο, και συγκεκριμένα 26 από την Calea jamaicensis, 12 από την Centaurea papposa, 24 από το Hypericum jovis, και 22 από την Euphorbia deflexa. Η ανίχνευση και απομόνωση μεταβολιτών, που ανήκουν στις κατηγορίες των σεσκιτερπενικών λακτονών, φλορογλουκινών και διτερπενίων ήταν κατευθυνόμενη μέσω 1Η NMR (Φασματοσκοπία Πυρηνικού Μαγνητικού Συντονισμού). Οι πορείες απομόνωσης περιελάμβαναν μία πληθώρα χρωματογραφικών τεχνικών, όπως στήλης με διαφορετικά πληρωτικά υλικά (πχ. silica, diaion, sephadex), παρασκευαστική χρωματογραφία λεπτής στιβάδας, καθώς και υγρή χρωματογραφία υψηλής απόδοσης (HPLC) με ανιχνευτές RI και DAD και κατάλληλες στήλες (RP-C18, RP-Biphenyl, NP). Οι χημικές δομές αποδώθηκαν με συνδυασμό από δεδομένα NMR, φασματομετρία μάζης (HRESIMS) και κρυσταλλογραφίας (X-ray). Απομονώθηκαν και ταυτοποιήθηκαν 28 σεσκιτερπενικές λακτόνες, 14 φλορογλουκινόλες, 22 διτερπένια και 20 άλλα συστατικά, μεταξύ αυτών 14 σεσκιτερπενικές λακτόνες, 2 φλορογλουκινόλες, 16 διτερπένια και 3 χρωμένια αποτελούν νέα φυσικά προϊόντα, που περιγράφονται για πρώτη φορά στη παρούσα εργασία, όπως και τα κρυσταλλογραφικά δεδομένα από δύο διτερπένια. Παράλληλα, περισσότερα από 120 πτητικά συστατικά ταυτοποιήθηκαν με την τεχνική της αεριοχρωματογραφίας συζευγμένης με φασματομετρία μάζης (GC-MS) από φυτά του γένους Hypericum (H. jovis, H. empetrifolium, H. amblyocalyx, H. triquetrifolium και H. perforatum), κυρίως μονοτερπενικοί και σεσκιτερπενικοί υδρογονάνθρακες. Οι απομονωμένες σεσκιτερπενικές λακτόνες αξιολογήθηκαν in vitro σε καρκινικές σειρές, παρουσιάζοντας IC50 ≤ 10 μM, τιμές που κατατάσσουν τα μόρια σε δραστικά και πιθανά αντικαρκινικά φάρμακα με βάση τις οδηγίες από το Εθνικό Ινστιτούτο Καρκίνου (National Cancer Institute). Συγκεκριμένα, ο carbetolide C απέδωσε τιμές IC50 για τις καρκινικές σειρές HeLa, SK-MEL-28 και HePG2 παρόμοιες με αυτές του παρθενολιδίου (2.9, 7.5, 12.6 μΜ και 2.1, 4.9, 10.0 μΜ, αντίστοιχα), που αποτελεί ισχυρή αντικαρκινική σεσκιτερπενική λακτόνη και η οποία χρησιμοποιήθηκε ως πρότυπη ουσία-μάρτυρας (positive control). Είναι σημαντικό πως στο σύνολό τους οι σεσκιτερπενικές λακτόνες δεν παρουσίασαν τοξικότητα στα φυσιολογικά/μη καρκινικά κύτταρα HMEC-1, παρότι οι αντιδράσεις τύπου Michael του λακτονικού δακτυλίου συνήθως περιλαμβάνουν και φαινόμενα μη ειδικής τοξικότητας. Στην κυτταρρική σειρά HMEC-1, οι σεσκιτερπενικές λακτόνες οδήγησαν σε δοσοεξαρτώμενη αντιφλεγμονώδη δράση αναστέλλοντας την επαγόμενη μέσω TNF-α έκφραση του μορίου ICAM-1. Ισχυρή αντιφλεγμονώδη δράση παρουσίασαν επίσης και οι φλορογλουκινόλες τόσο στην in vitro βιοδοκιμασία του ICAM-1, όσο και στην ex vivo βιοδοκιμασία COX/LOX. Πολύ λίγες μελέτες περιγράφουν τις αντιφλεγμονώδεις ιδιότητες αυτών των ουσιών, καταδεικνύοντας την ανάγκη για περαιτέρω διερεύνηση της δυναμικής τους ως αντιφλεγμονώδεις παράγοντες. Όσον αφορά στα διτερπένια από την Euphorbia, αν και άφθονα με εξαιρετικό ενδιαφέρον στην περίπλοκη απόδοση δομών, τα περισσότερα από αυτά δεν παρουσίασαν αξιόλογες βιολογικές δράσεις. Περαιτέρω, αν και είναι γνωστή η επουλωτική δράση του βαλσαμέλαιου (Oleum Hyperici), δεν είχαν μελετηθεί προηγουμένως οι επουλωτικές ιδιότητες των αιθερίων ελαίων από φυτά του γένους Hypericum, παρότι πτητικά συστατικά συνεκχυλίζονται κατά την παρασκευή του βαλσαμέλαιου. Για να καλυφθεί αυτό το κενό, διερευνήθηκαν in vivo πέντε αιθέρια έλαια και έδειξαν σημαντική επουλωτική δράση, ενισχύοντας τη γνώση για τις θεραπευτικές ιδιότητες του γένους Hypericum. Επιπλέον, ο μεταβολισμός των φυσικών προϊόντων είναι πολύ λιγότερο μελετημένος σε σχέση με τα βιοσυνθετικά τους μονοπάτια, οπότε ένας τελικός στόχος της παρούσας διατριβής αποτέλεσε η διερεύνηση του in vitro μεταβολισμού των σεσκιτερπενικών λακτονών χρησιμοποιώντας μικροσώματα ανθρώπινου ήπατος και οι μεταβολίτες ανιχθεύθηκαν με LC-MS/MS. Οι σεσκιτερπενικές λακτόνες οδήγησαν σε ποικίλους μεταβολίτες λόγω της δομικής τους ποικιλομορφίας, αν και ο εξαιρετικά ενεργός δακτύλιος λακτόνης παρέμεινε η πιο κοινή μεταβολική θέση.Συμπερασματικά, αναφέρθηκε υψηλή κυτταροτοξική, αντιφλεγμονώδης και επουλωτική δράση από φυσικά προϊόντα που προέρχονται από τα φυτά που μελετήθηκαν, i.e. Calea jamaicensis, Centaurea papposa, Hypericum jovis, H. empetrifolium και Euphorbia deflexa. Για αυτά τα είδη, υπήρχαν ελάχιστες αναφορές στη βιβλιογραφία. Στη παρούσα διδακτoρική διατριβή μελετήθηκαν αυτά τα είδη χρησιμοποιώντας σύγχρονες τεχνικές και διερευνώντας τις βιολογικές επιδράσεις των συστατικών τους. Η φύση θα συνεχίσει να αποτελεί μια πηγή νέων φαρμάκων στο μέλλον και τα αποτελέσματα αυτής της μελέτης συμβάλλουν στην ανακάλυψη δραστικών ουσιών βασισμένων στα φυσικά προϊόντα, υποστηρίζοντας περαιτέρω τη στρατηγική χρήση φυσικών προϊόντων ως αφετηρία για τη διερεύνηση νέων δομών ως δυνητικών παραγόντων για ποικίλες ανθρώπινες παθήσεις.

Inhibitory Effects of BiRyuChe-Bang on Mast Cell-Mediated Allergic Reactions and Inflammatory Cytokines Production

2013 ◽  
Vol 41 (06) ◽  
pp. 1267-1282 ◽  
Author(s):  
Phil-Dong Moon ◽  
Il Sang Choi ◽  
Ji-Hyun Go ◽  
Byong-Joo Lee ◽  
Sang Woo Kang ◽  
...  
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Histamine Release ◽  
Inflammatory Cytokines ◽  
Medical Center ◽  
Human Mast Cell ◽  
Enzyme Linked Immunosorbent Assay ◽  
Allergic Reactions ◽  
Linalyl Acetate ◽  
Tnf Α

BiRyuChe-bang (BRC) is a Korean prescription medicine, which has been used to treat allergic rhinitis at Kyung Hee Medical Center. In this work, we investigated the effects of BRC on mast cell-mediated allergic reactions and inflammatory cytokines production, and identified the active component of BRC. Histamine release was measured from rat peritoneal mast cells (RPMCs). Ear swelling and passive cutaneous anaphylaxis (PCA) were examined in mouse models. Phorbol 12-myristate 13-acetate (PMA) plus A23187-induced inflammatory cytokines production was measured using enzyme-linked immunosorbent assay. Reverse transcriptase-polymerase chain reaction was used for the expressions of tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-8. Activation of nuclear factor (NF)-κB was analyzed by Western blotting. BRC significantly inhibited the compound 48/80-induced ear swelling response, histamine release from RPMCs, PCA activated by anti-dinitrophenyl IgE, and PMA plus A23187-induced inflammatory cytokines production (p < 0.05). In addition, BRC dose-dependently inhibited the mRNA expressions of TNF-α, IL-6, and IL-8 as well as the activation of NF-κB in a human mast cell line, HMC-1 cells. BRC inhibited the levels of TNF-α and IL-6 in mice induced with PCA. Several components of BRC, such as 1,8-Cineole, Linalool, Linalyl acetate, α-Pinene, and α-Terpineol, significantly inhibited the release of histamine from RPMCs (p < 0.05). Among these components, Linalyl acetate was the most effective for inhibiting histamine release. These results indicate that BRC has a potential regulatory effect on allergic and inflammatory reactions mediated by mast cells.

scholarly journals Chemical Constituents of the Deep-Sea-Derived Penicillium solitum

Marine Drugs ◽  
2021 ◽  
Vol 19 (10) ◽  
pp. 580
Author(s):  
Zhi-Hui He ◽  
Jia Wu ◽  
Lin Xu ◽  
Man-Yi Hu ◽  
Ming-Ming Xie ◽  
...  
Keyword(s):  
Deep Sea ◽  
Chemical Constituents ◽  
A549 Cells ◽  
Acid Methyl Ester ◽  
2D Nmr ◽  
Positive Control ◽  
Ic50 Values ◽  
Penicillium Solitum ◽  
New Compounds

A systematic chemical investigation of the deep-sea-derived fungus Penicillium solitum MCCC 3A00215 resulted in the isolation of one novel polyketide (1), two new alkaloids (2 and 3), and 22 known (4–25) compounds. The structures of the new compounds were established mainly on the basis of exhaustive analysis of 1D and 2D NMR data. Viridicatol (13) displayed moderate anti-tumor activities against PANC-1, Hela, and A549 cells with IC50 values of around 20 μM. Moreover, 13 displayed potent in vitro anti-food allergic activity with an IC50 value of 13 μM, compared to that of 92 μM for the positive control, loratadine, while indole-3-acetic acid methyl ester (9) and penicopeptide A (10) showed moderate effects (IC50 = 50 and 58 μM, respectively).

scholarly journals Moringa Leaf Powder (Moringa oleifera) Decrease of Inflammation Plasma Cytokine of Pregnant Rats with Diabetes Mellitus

2021 ◽  
Vol 9 (A) ◽  
pp. 1043-1046
Author(s):  
Harry Kurniawan Gondo
Keyword(s):  
Diabetes Mellitus ◽  
Risk Factors ◽  
Dose Group ◽  
Moringa Oleifera ◽  
Normal Pregnancy ◽  
Positive Control ◽  
Control Group ◽  
Group 4 ◽  
Tnf Α ◽  
Leaf Powder

BACKGROUND: Preeclampsia is a major obstetric problem worldwide, especially in developing countries that can cause maternal and fetal morbidity and mortality that affect 2–10% of pregnancies worldwide. More than 4 million pregnant women each year develop preeclampsia and cause 15–20% of maternal deaths worldwide. There are several high-risk factors associated with preeclampsia. Diabetes mellitus is one of the risk factors for preeclampsia. Preeclampsia has been shown to have higher levels of Th1 (pro-inflammatory) products and lower levels of Th2 (anti-inflammatory) products compared to normal pregnancy in blood serum. Interleukin (IL) 1, IL-2, IL-8, tumor necrosis factor (TNF)-alpha, and interferon gamma are Th1 cytokines or pro-inflammatory cytokines that can induce inflammatory reactions and are associated with pregnancy complications such as repeated abortion, preterm labor, rupture of membranes. preeclampsia, and stunted fetal growth. In contrast, Th2 cytokines such as transforming growth factor-β (TGF-β), IL-4, IL-5, IL6, and IL-10 are associated with normal pregnancy. AIM: This study aims to prove the effect of giving Moringa leaf powder (M. oleifera) to decrease blood levels, , so this study will answer the effect of Moringa oleifera leaf powder on blood level of IL-6, IL-10, TNF-α and TGF-β METHODS: A total of 30 rats were checked for sugar levels on day 4 after being induced by alloxan for 18 days to see if the rats were already in a hyperglycemic state. Then, they were divided into six groups, each group contained five pregnant white rats. Group 1 was the negative group (without being given alloxan), Group 2 was positive (given alloxan 150 mg/day/kg BW), and Groups 3, 4, 5, and 6 were given alloxan 150 mg/day/kg BW and each given a dose of 100 Moringa leaf powder; 200, 400, and 800 mg/day/kg body weight. After alloxan was induced and given a dose of Moringa leaf powder according to each group, blood samples were taken to separate the serum and continued with the ELISA method to calculate the levels of IL-6, IL-10, TNF-α, and TGF-β. RESULTS: The administration of Moringa leaf powder at a dose of 800 mg/day/kg BW was able to increase the levels of IL-10 in the positive control Group 4 by 7.211 mIU/ml and reduced levels of IL-6 in the 8th dose group by giving Moringa leaf powder as much as 800 mg/day/kg body weight by 2.112 mIU/ml, was able to increase TGF-β levels in the dose Group 4 by giving Moringa leaf powder as much as 800 mg/day/kg BW, amounted to 1049.066 mIU/ml, and decreased TNF-α levels in the dose Group 4 by giving Moringa leaf powder as much as 800 mg/day/kg BW mIU/ml. CONCLUSION:The administration of Moringa leaf powder was able to increase the levels of IL-10 in the positive control Group, reduced levels of IL-6, was able to increase TGF-β levels, and decreased TNF-α levels.

scholarly journals Anti-Food Allergic Compounds from Penicillium griseofulvum MCCC 3A00225, a Deep-Sea-Derived Fungus

Marine Drugs ◽  
2021 ◽  
Vol 19 (4) ◽  
pp. 224
Author(s):  
Cui-Ping Xing ◽  
Dan Chen ◽  
Chun-Lan Xie ◽  
Qingmei Liu ◽  
Tian-Hua Zhong ◽  
...  
Keyword(s):  
Deep Sea ◽  
Spectroscopic Data ◽  
Positive Control ◽  
X Ray ◽  
X Ray Crystallography ◽  
Ic50 Value ◽  
Penicillium Griseofulvum ◽  
New Compounds ◽  
Basophilic Leukemia

Ten new (1–10) and 26 known (11–36) compounds were isolated from Penicillium griseofulvum MCCC 3A00225, a deep sea-derived fungus. The structures of the new compounds were determined by detailed analysis of the NMR and HRESIMS spectroscopic data. The absolute configurations were established by X-ray crystallography, Marfey’s method, and the ICD method. All isolates were tested for in vitro anti-food allergic bioactivities in immunoglobulin (Ig) E-mediated rat basophilic leukemia (RBL)-2H3 cells. Compound 13 significantly decreased the degranulation release with an IC50 value of 60.3 μM, compared to that of 91.6 μM of the positive control, loratadine.

scholarly journals Two new compounds from leaves of Bruguiera cylindrica (L.) Blume with the in vitro α-glucosidase inhibitory activity

2021 ◽  
Vol 23 (4) ◽  
Author(s):  
Thuy Thi Le Nguyen ◽  
Tung Thanh Bui ◽  
Phung Kim Phi Nguyen ◽  
Chi Minh Tran ◽  
Tu Dang Cam Phan ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Ethyl Acetate Extract ◽  
Chemical Constituents ◽  
Positive Control ◽  
Chemical Structures ◽  
Ic50 Values ◽  
Bruguiera Cylindrica ◽  
New Compounds ◽  
Better Than

Introduction: Bruguiera cylindrica is one of the mangrove plants belonging to Bruguiera genus. This genus is characterized by the presence of a large number of compounds, but the research on bioactivities has not been investigated so far. In the present research, the α-glucosidase inhibitory activity, as well as chemical constituents of the ethyl acetate extract of this plant, were studied. Methods: The chemical structures of two new compounds were elucidated by spectroscopic and computational methods. Results: Two new compounds, benzobrugierol (1) and bruguierine (2), were isolated from leaves of Bruguiera cylindrica (L.) Blume, together with nine known ones, including lupeol (3), betulin (4), chrysoeriol (5), glut-5-ene-3-ol (6), cholesta-4-ene-3-one (7), 3α-(Z)-coumaroyllupeol (8), 3α-(E)-coumaroyllupeol (9), 3β-hydroxycholesta-5-ene-7-one (10) and β-sitosterol 3-O-β-D-glucopyranoside (11). Extracts and some isolated compounds were evaluated for α-glucosidase inhibitory activities. Conclusion: The results showed that most of the extracts and tested compounds exhibited activities better than the positive control acarbose, especially two new compounds 1 and 2 with their IC50 values of 17.9 ± 0.4 and 34.6 ± 0.7 (mg/mL), respectively.

scholarly journals Phenolic Glycosides from Lindera obtusiloba and their Anti-allergic Inflammatory Activities

2013 ◽  
Vol 8 (2) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Hyun Gyu Choi ◽  
Hwa Dong Lee ◽  
Sang Hyun Kim ◽  
Min Kyun Na ◽  
Jeong Ah Kim ◽  
...  
Keyword(s):  
Mast Cells ◽  
Histamine Release ◽  
Proinflammatory Cytokines ◽  
Allergic Inflammation ◽  
Positive Control ◽  
Phenolic Glycosides ◽  
Gene Expressions ◽  
Human Mast Cells ◽  
Tnf Α ◽  
Lindera Obtusiloba

Eight phenolic glycosides, tachioside (1), isotachioside (2), koaburaside (3), 2,6-dimethoxy-4-hydroxyphenyl-1- O-ß-D-glucopyranoside (4), 4,6-dihydroxy-2- methoxyphenyl-1- O-β-D-glucopyranoside (5), a mixture of erigeside C (6a) and salidroside (6b), and 6-hydroxyphenyl)-1- O-β-D-glucopyranoside (7) were isolated from the stems of Lindera obtusiloba Blume. The structures of the isolates were determined by 1H-, 13C-NMR, COSY, HMQC, and HMBC spectroscopy. To evaluate their anti-allergic inflammatory activities, the inhibitory effects of isolates (1-7) on histamine release and on the gene expressions of tumor necrosis factor (TNF)-α and interleukin (IL)-6 were examined using human mast cells; previous studies have reported that TNF-α and IL-6 release from mast cells is positively related to the severity of allergic symptoms. Of the tested compounds, koaburaside (3), 2,6-dimethoxy-4-hydroxyphenyl-1- O-ß-D- glucopyranoside (4), and (6-hydroxyphenyl)-1- O-β-D-glucopyranoside (7) suppressed histamine release from mast cells as compared with gallic acid (positive control). In particular, 6-hydroxyphenyl)-1- O-β-D-glucopyranoside (7) attenuated the gene expressions of the proinflammatory cytokines TNF-α and IL-6 in human mast cells. Our results support the notion that phenolic glycosides isolated from L. obtusiloba inhibit mast-cell-derived allergic inflammation, histamine, and proinflammatory cytokines.

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