scholarly journals Synthesis and self-assembly of curcumin-modified amphiphilic polymeric micelles with antibacterial activity

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Caio H. N. Barros ◽  
Dishon W. Hiebner ◽  
Stephanie Fulaz ◽  
Stefania Vitale ◽  
Laura Quinn ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Self Assembly ◽  
Glycolic Acid ◽  
Extracellular Polymeric Substances ◽  
Polymeric Micelles ◽  
Polymeric Nanoparticles ◽  
Enhanced Resistance ◽  
Antimicrobial Nanoparticles ◽  
Good Penetration ◽  
Antibiofilm Agents

Abstract Background The ubiquitous nature of bacterial biofilms combined with the enhanced resistance towards antimicrobials has led to the development of an increasing number of strategies for biofilm eradication. Such strategies must take into account the existence of extracellular polymeric substances, which obstruct the diffusion of antibiofilm agents and assists in the maintenance of a well-defended microbial community. Within this context, nanoparticles have been studied for their drug delivery efficacy and easily customised surface. Nevertheless, there usually is a requirement for nanocarriers to be used in association with an antimicrobial agent; the intrinsically antimicrobial nanoparticles are most often made of metals or metal oxides, which is not ideal from ecological and biomedical perspectives. Based on this, the use of polymeric micelles as nanocarriers is appealing as they can be easily prepared using biodegradable organic materials. Results In the present work, micelles comprised of poly(lactic-co-glycolic acid) and dextran are prepared and then functionalised with curcumin. The effect of the functionalisation in the micelle’s physical properties was elucidated, and the antibacterial and antibiofilm activities were assessed for the prepared polymeric nanoparticles against Pseudomonas spp. cells and biofilms. It was found that the nanoparticles have good penetration into the biofilms, which resulted in enhanced antibacterial activity of the conjugated micelles when compared to free curcumin. Furthermore, the curcumin-functionalised micelles were efficient at disrupting mature biofilms and demonstrated antibacterial activity towards biofilm-embedded cells. Conclusion Curcumin-functionalised poly(lactic-co-glycolic acid)-dextran micelles are novel nanostructures with an intrinsic antibacterial activity tested against two Pseudomonas spp. strains that have the potential to be further exploited to deliver a secondary bioactive molecule within its core. Graphic Abstract

scholarly journals High Throughput Preparation of Poly(Lactic-Co-Glycolic Acid) Nanoparticles Using Fiber Fluidic Reactor

Materials ◽  
2020 ◽  
Vol 13 (14) ◽  
pp. 3075
Author(s):  
Niloofar Heshmati Aghda ◽  
Emilio J. Lara ◽  
Pulinkumar Patel ◽  
Tania Betancourt
Keyword(s):  
High Throughput ◽  
Self Assembly ◽  
Large Scale ◽  
Glycolic Acid ◽  
Polymeric Nanoparticles ◽  
High Surface ◽  
High Surface Area ◽  
Poly Vinyl Alcohol ◽  
Operational Parameters ◽  
Operational Temperature

Polymeric nanoparticles (NPs) have a variety of biomedical, biotechnology, agricultural and environmental applications. As such, a great need has risen for the fabrication of these NPs in large scales. In this study, we used a high throughput fiber reactor for the preparation of poly(lactic-co-glycolic acid) (PLGA) NPs via nanoprecipitation. The fiber reactor provided a high surface area for the controlled interaction of an organic phase containing the PLGA solution with an aqueous phase, containing poly(vinyl alcohol) (PVA) as a stabilizer. This interaction led to the self-assembly of the polymer into the form of NPs. We studied operational parameters to identify the factors that have the greatest influence on the properties of the resulting PLGA NPs. We found that the concentration of the PLGA solution is the factor that has the greatest effect on NP size, polydispersity index (PDI), and production rate. Increasing PLGA concentration increased NP sizes significantly, while at the same time decreasing the PDI value. The second factor that was found to affect NP properties was the concentration of PVA solution, which resulted in increased NP sizes and decreased production rates. Flowrates of the feed streams also affected NP size to a lesser extent, while changing the operational temperature did not change the product’s features. In general, the results demonstrate that fiber reactors are a suitable method for the large-scale, continuous preparation of polymeric NPs suitable for biomedical applications.

Peptide-Directed Self-Assembly of Functionalized Polymeric Nanoparticles Part I: Design and Self-Assembly of Peptide-Copolymer Conjugates into Nanoparticle Fibers and 3D Scaffolds

2014 ◽  
Vol 14 (6) ◽  
pp. 853-871 ◽  
Author(s):  
Xiaochu Ding ◽  
Jagadeesh Janjanam ◽  
Ashutosh Tiwari ◽  
Martin Thompson ◽  
Patricia A. Heiden
Keyword(s):  
Self Assembly ◽  
Polymeric Nanoparticles ◽  
3D Scaffolds

Current phthalocyanines delivery systems in photodynamic therapy: an updated review

2021 ◽  
Vol 28 ◽  
Author(s):  
Mariana Miretti ◽  
Cesar German Prucca ◽  
Tomas Cristian Tempesti ◽  
Maria Teresa Baumgartner
Keyword(s):  
Photodynamic Therapy ◽  
Polymeric Micelles ◽  
Polymeric Nanoparticles ◽  
Aqueous Media ◽  
Delivery Systems ◽  
Current Status ◽  
Oxygen Species ◽  
Cancer Treatments ◽  
Therapy Efficacy ◽  
Cellular Components

: Photodynamic therapy has emerged as an effective therapeutic alternative to treat oncological, cardiovascular, dermatological, infectious, and ophthalmic diseases. Photodynamic therapy combines the action of a photosensitizer with light in the presence of oxygen to generate reactive oxygen species capable of reacting with cellular components resulting in injury and, consequently, inducing cellular death. Phthalocyanines are considered good photosensitizers, although most of them are lipophilic, difficulting their administration for clinical use. A strategy to overcome the lack of solubility of phthalocyanines in aqueous media is to incorporate them into different delivery systems. The present review aimed to summarize the current status of the main drug delivery systems used for Zn and Al phthalocyanines and their effect in photodynamic therapy, reported in the last five years. Liposomes, polymeric micelles, polymeric nanoparticles, and gold-nanoparticles constituted some of the most used carriers and were discussed in this review. The latest studies reported strongly suggests that the application of nanotechnologies as delivery systems allow an increase in photodynamic therapy efficacy and reduce side-effects associated with the phthalocyanine administration, which represents a promise for cancer treatments.

scholarly journals Translating the fabrication of protein-loaded poly(lactic-co-glycolic acid) nanoparticles from bench to scale-independent production using microfluidics

2020 ◽  
Vol 10 (3) ◽  
pp. 582-593 ◽  
Author(s):  
Carla B. Roces ◽  
Dennis Christensen ◽  
Yvonne Perrie
Keyword(s):  
Particle Size ◽  
Glycolic Acid ◽  
Polymeric Nanoparticles ◽  
Physicochemical Characteristics ◽  
European Medicines Agency ◽  
Protein Encapsulation ◽  
Human Use ◽  
Rate Ratios ◽  
The Impact ◽  
Process Controls

AbstractIn the formulation of nanoparticles, poly(lactic-co-glycolic acid) (PLGA) is commonly employed due to its Food and Drug Administration and European Medicines Agency approval for human use, its ability to encapsulate a variety of moieties, its biocompatibility and biodegradability and its ability to offer a range of controlled release profiles. Common methods for the production of PLGA particles often adopt harsh solvents, surfactants/stabilisers and in general are multi-step and time-consuming processes. This limits the translation of these drug delivery systems from bench to bedside. To address this, we have applied microfluidic processes to develop a scale-independent platform for the manufacture, purification and monitoring of nanoparticles. Thereby, the influence of various microfluidic parameters on the physicochemical characteristics of the empty and the protein-loaded PLGA particles was evaluated in combination with the copolymer employed (PLGA 85:15, 75:25 or 50:50) and the type of protein loaded. Using this rapid production process, emulsifying/stabilising agents (such as polyvinyl alcohol) are not required. We also incorporate in-line purification systems and at-line particle size monitoring. Our results demonstrate the microfluidic control parameters that can be adopted to control particle size and the impact of PLGA copolymer type on the characteristics of the produced particles. With these nanoparticles, protein encapsulation efficiency varies from 8 to 50% and is controlled by the copolymer of choice and the production parameters employed; higher flow rates, combined with medium flow rate ratios (3:1), should be adopted to promote higher protein loading (% wt/wt). In conclusion, herein, we outline the process controls for the fabrication of PLGA polymeric nanoparticles incorporating proteins in a rapid and scalable manufacturing process.

scholarly journals Facile fabrication of Tl4HgI6 nanostructures as a novel antibacterial and antibiofilm agents

2020 ◽  
Author(s):  
Maryam Karami ◽  
Mojgan Ghanbari ◽  
Omid Amiri ◽  
MASOUD SALAVATI-NIASARI ◽  
Somaye Rashki
Keyword(s):  
Raman Spectroscopy ◽  
Antibacterial Activity ◽  
Bactericidal Activity ◽  
Stoichiometric Ratio ◽  
Raman Spectrometry ◽  
Gram Negative ◽  
Biofilm Production ◽  
Facile Fabrication ◽  
Antibiofilm Agents ◽  
The Impact

Abstract In the present study, Tl4HgI6 nanostructures have been successfully fabricated through a simple precipitation route. The impact of TlI stoichiometric ratio to HgI2, and kind of surfactants was explored on purity, structure, and shape of samples. The as-fabricated Tl4HgI6 was characterized via XRD, EDX, FESEM, TEM, HRTEM, and Raman spectroscopy. Raman spectrometry corroborated the XRD outcomes, and revealed that the Tl4HgI6 nanoparticles were successfully fabricated. The structure, shape, and scale of the products were studied through FESEM images. It was observed that different factors have a notable aspect on the morphology and size of the products. The maximum antibacterial activity of Tl4HgI6 was perceived against S. aureus, E.coli and M. catarils. These outcomes demonstrate that Tl4HgI6 displays efficient bactericidal activity against Gram-positive and Gram-negative microorganisms. The anti-biofilm activity revealed that the best reduction of biofilm was recognized in higher Tl4HgI6 concentrations (2×MIC). Tl4HgI6 at 2×MIC concentration inhibits biofilm production by S. aureus and E. feacalis with an inhibition percentage of 95% and 90%, respectively.

Self-Assembly of Virulent Amyloid-Derived Peptides into Nanoantibacterials

Nanoscale ◽  
2021 ◽  
Author(s):  
Wenlu Tu ◽  
Ke Xue ◽  
Shaofeng Lou ◽  
Chunlei Zhu ◽  
Zhilin Yu
Keyword(s):  
Antibacterial Activity ◽  
Self Assembly ◽  
Antibacterial Peptides

Current strategies for design of antibacterial peptides show limitation in development of assembled antibacterial peptides due to the challenges in simultaneously balancing the antibacterial activity and assembling behavior. Herein, we...

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