scholarly journals Comparison of RNAscope and immunohistochemistry for evaluation of the UPK2 status in urothelial carcinoma tissues

2022 ◽  
Vol 17 (1) ◽  
Author(s):  
Jiangli Lu ◽  
Ming Zhao ◽  
Chenyan Wu ◽  
Chengbiao Chu ◽  
Chris Zhiyi Zhang ◽  
...  
Keyword(s):  
Correlation Analysis ◽  
Urothelial Carcinoma ◽  
Correct Diagnosis ◽  
Diagnostic Sensitivity ◽  
Positive Correlation ◽  
Upper Tract ◽  
Significant Difference ◽  
Positive Rate ◽  
Low Sensitivity ◽  
Moderate Positive Correlation

Abstract Background UPK2 exhibits excellent specificity for urothelial carcinoma (UC). UPK2 evaluation can be useful in making the correct diagnosis of UC. However, UPK2 detection by immunohistochemistry (IHC) has relatively low sensitivity. This paper aimed to compare the diagnostic sensitivity of RNAscope and IHC for evaluation of the UPK2 status in UC. Methods Tissue blocks from 127 conventional bladder UCs, 45 variant bladder UCs, 24 upper tract UCs and 23 metastatic UCs were selected for this study. IHC and RNAscope were used to detect the UPK2 status in UCs. Then, comparisons of the two methods were undertaken. Results There was no significant difference between RNAscope and IHC for the evaluation of the UPK2 positivity rate in UC (68.0% vs. 62.6%, P = 0.141). Correlation analysis revealed a moderate positive correlation for detection of UPK2: RNAscope vs. IHC (P < 0.001, R = 0.441). Our results showed a trend toward a higher positive UPK2 rate detected by RNAscope (53.3%) than by IHC (35.6%) in variant bladder UCs. Disappointingly, the P value did not indicate a significant difference (P = 0.057). Conclusions RNAscope for UPK2 appeared to perform similarly to IHC, with a marginally higher positive rate, suggesting it could be used as an alternative or adjunct to UPK2 IHC.

scholarly journals Use of the Nuclear Matrix Protein 22 BladderChek Test for the Detection of Primary and Recurrent Urothelial Carcinoma

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Chang-sheng Xia ◽  
Chun-hong Fan ◽  
Ming Su ◽  
Qing-song Wang ◽  
Hui-zhang Bao
Keyword(s):  
Urothelial Carcinoma ◽  
Sensitivity And Specificity ◽  
Nuclear Matrix ◽  
Matrix Protein ◽  
Benign Disease ◽  
Urine Cytology ◽  
Nuclear Matrix Protein ◽  
Combined Use ◽  
Significant Difference ◽  
Low Sensitivity

Objective. To evaluate the performance of the nuclear matrix protein 22 (NMP22) BladderChek test in urothelial carcinoma (UC). Methods. We retrospectively analyzed 1318 patients who performed the NMP22 BladderChek tests. Of them, 103 were primary UC patients, 90 were surgical treatment UC patients, and 1125 were benign disease patients. The performance of the NMP22 BladderChek test for the diagnosis of primary and recurrent UC was evaluated. Moreover, the performance of urine cytology and the NMP22 BladderChek test for the diagnosis of primary UC was compared in 90 available subjects including 48 primary UC patients and 42 benign disease patients. Results. The sensitivity and specificity of the NMP22 BladderChek test were 37.9% and 95.8%, respectively, for the diagnosis of primary UC (n=1228). The corresponding parameters of the NMP22 BladderChek test were 31.0% and 88.5%, respectively, for the diagnosis of recurrent UC (n=90). The sensitivity and specificity of urine cytology were 54.2% and 97.6%, respectively, for the diagnosis of primary UC (n=90); the corresponding parameters of the NMP22 BladderChek test were 41.7% and 83.3%, respectively; the corresponding parameters of the two tests combination were 64.6% and 83.3%, respectively. There was a significant difference in the performance between the NMP22 BladderChek test and urine cytology or the combination of two tests (P=0.017 and 0.001, respectively). Conclusions. The NMP22 BladderChek test has a low sensitivity for detecting primary and recurrent UC. Urine cytology is superior to the NMP22 BladderChek test, and combined use of the two tests improves the sensitivity in the detection of primary UC.

Correlation of MECA-79-positive high endothelial venule density with clinical outcomes in upper tract urothelial carcinoma patients treated with radical nephroureterectomy.

2018 ◽  
Vol 36 (6_suppl) ◽  
pp. 417-417
Author(s):  
Yasuhiro Hashimoto ◽  
Hayato Yamamoto ◽  
Tohru Yoneyama ◽  
Atsushi Imai ◽  
Shingo Hatakeyama ◽  
...  
Keyword(s):  
Urothelial Carcinoma ◽  
Clinical Outcomes ◽  
Poor Prognosis ◽  
Disease Free Survival ◽  
Small Sample ◽  
Upper Tract Urothelial Carcinoma ◽  
Radical Nephroureterectomy ◽  
Upper Tract ◽  
Significant Difference ◽  
The Mean

417 Background: High endothelial venules (HEVs) are present in lymph nodes and tertiary lymphoid organs. It has been reported that low HEV density is associated with the poor prognosis of several carcinomas. MECA-79 antibody recognizes L-selectin ligand (6-sulfosialyl Lewis X glycan) expressed in HEV. In the present study, we examined whether MECA-79 positive HEV density was associated with clinical outcomes of patients with upper tract urothelial carcinoma (UTUC) treated with radical nephroureterectomy (RNU). Methods: Eighty-eight patients with UTUC who underwent RNU at the Hirosaki University hospital between January 2008 and December 2016 were enrolled. Tissue microarray for MECA-79 was performed, and HEV densities were calculated. HEV density < 1.5/mm2 was defined as HEV (−); HEV density ≥1.5/mm2 was defined as HEV(+). Results: Of 88 patients, 64 (72.7%) were male and 24 (27.2%) were female. The average age was 68.5 years (range, 36–84 years). Fifty-three patients (60.2%) had previously undergone neoadjuvant chemotherapy. The mean observation period was 39.0 months. Twenty-one (23.8%) patients developed recurrence, whereas 16 (33.3%) patients died during follow-up. Five-year cause-specific survival (CSS) rate was 66.1%, and five-year disease-free survival (DFS) rate was 70.7%. In our cohort, 25 (28.4%) patients were found to be HEV(−), whereas 63 (71.5%) were found to be HEV(+). The mean HEV density was 6.3/mm2(0-41.6). The 5-year DFS rates for HEV (+) and HEV (−) patients were 78.0% and 53.9%, respectively, with a statistically significant difference between the groups. (log-rank, p = 0.042). Moreover, the 5-year CSS rates for HEV (+) and HEV (−) patients were 72.5% and 53.4%, respectively, with a statistically significant difference between the groups. (log-rank, p = 0.0036). Conclusions: Low MECA-79-positive HEV density may be associated with poor prognosis of patients with UTUC treated with RNU. Despite the small sample size and preliminary nature of our study, our study provides valuable insights to guide future research.

Prognostic role of programmed cell death protein 1 expression in surgically treated patients with upper tract urothelial carcinoma.

2016 ◽  
Vol 34 (2_suppl) ◽  
pp. 402-402
Author(s):  
Nozomi Hayakawa ◽  
Eiji Kikuchi ◽  
Ryuichi Mizuno ◽  
Keishiro Fukumoto ◽  
Takeo Kosaka ◽  
...  
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Protein Expression ◽  
Urothelial Carcinoma ◽  
Lymphovascular Invasion ◽  
Upper Tract Urothelial Carcinoma ◽  
Upper Tract ◽  
Significant Difference ◽  
Cell Death Protein

402 Background: Programmed cell death protein (PD-1) expressed on active T cells, and its ligand PD-L1 expressed on the surface of cancer cells, complementarily down-regulate T cell activation and are related to immune tolerance. A close association between PD-1 expression and poor prognosis has been reported in several cancers, however, in upper tract urothelial carcinoma (UTUC) the role of PD-1 expression on clinical outcome has not been investigated. Methods: The protein expression of PD-1 was evaluated by immunohistochemistry and the relationship with clinicopathological features was investigated in surgical specimens obtained from 100 patients who had been surgically treated for UTUC. At a magnification of 200x, PD-1 protein expression was estimated and the positive cells were graded as no (negative), moderate (1-10 cells), and strong ( > 10 cells). Results: Twenty-four patients (24.0%) had strong PD-1 staining, 32 patients (32.0%) had moderate PD-1 staining, and 44 patients (44.0%) had no PD-1 staining. PD-1 staining was associated with pathological T stage (p = 0.023), tumor grade (p = 0.005), and lymphovascular invasion (p = 0.033). Lymphovascular invasion (p < 0.001) and PD-1 staining (p = 0.02) were independent factors for predicting disease metastasis. The 5-year matastatic free survival rate in patients with strong PD-1 staining was 57.3 %, which was significantly lower than that with no PD-1 staining (87.3%, p=0.001) and that with moderate PD-1 staining (74.3%, p = 0.05). In a sub-group analysis of patients with ≥pT2 (N = 59), a significant difference in disease metastasis was observed between patients with strong PD-1 staining and no PD-1 staining (p = 0.018), but was not observed between strong and moderate PD-1 staining (p = 0.146). Conclusions: PD-1 expression may be a useful indicator for a worse prognosis in UTUC patients who undergo radical nephroureterectomy. Targeting therapy against PD-1 might be a promising therapeutic modality for UTUC.

scholarly journals Comparison of Radical Nephroureterectomy and Partial Ureterectomy for the Treatment of Upper Tract Urothelial Carcinoma

2018 ◽  
Vol 2018 ◽  
pp. 1-7
Author(s):  
Jianzhong Zhang ◽  
Feiya Yang ◽  
Mingshuai Wang ◽  
Yinong Niu ◽  
Weicheng Chen ◽  
...  
Keyword(s):  
Urothelial Carcinoma ◽  
Kidney Function ◽  
Operation Time ◽  
Upper Tract Urothelial Carcinoma ◽  
Radical Nephroureterectomy ◽  
Cox Regression Analysis ◽  
Upper Tract ◽  
Significant Difference ◽  
The Mean ◽  
Kidney Functions

This study aimed to compare the oncological and renal outcomes of partial ureterectomy (PU) versus radical nephroureterectomy (RNU) for upper tract urothelial carcinoma (UTUC). UTUC patients’ clinical information was reviewed, and progression-free survival (PFS), overall survival (OS), and kidney function were collected. The mean follow-up period was 59 (6–135) months in the RNU group and 34.5 (5–135) months in the PU group. The mean operation time in the PU group was 141 (64–340) min, which is significantly shorter than the RNU group (P<0.01). Regarding kidney function at one year or two years after operation, the PU group had significantly improved mean estimated glomerular filtration rate (eGFR) levels and a remarkably decreased constitution of patients with chronic kidney disease (CKD) III or higher group (P<0.05). There was no significant difference in PFS and OS between the RNU group and the PU group (P>0.05). Multifactor Cox regression analysis indicated that age and the preoperative CKD stages were independent risk factors for poor kidney functions of UTUC patients. Compared to patients in RNU group, patients in PU have no significant difference in survival time but have shorter operation time, shorter hospital stay, and improved kidney functions.

scholarly journals Correlation between Beliefs on Translation and the Use of It as an English Learning Strategy

2020 ◽  
Vol 1 (02) ◽  
pp. 196-209
Author(s):  
Mega Fariziah Nur Humairoh ◽  
Febrina Rizky Agustina
Keyword(s):  
Correlation Analysis ◽  
Undergraduate Students ◽  
Learning Strategy ◽  
T Test ◽  
Correlational Study ◽  
English Learning ◽  
Strong Positive Correlation ◽  
Positive Correlation ◽  
Significant Difference ◽  
Efl Students

This current study focuses its investigation on the correlation between students’ beliefs about the use of translation as an English learning strategy and the strategic use of translation in learning English by EFL students in Indonesia. This current study is aimed 1) to investigate the correlation between students’ beliefs and the use of translation as a learning strategy 2) to examine the developmental of the use of translation as a learning strategy across different year of study. One hundred seventeen undergraduate students, consisting 67 freshmen and 50 sophomores participated in this correlational study. The data were collected by frequency of an Indonesian Version of the Inventory for Beliefs about Translation for beliefs measurement and the Inventory for Translation as a Learning Strategy. Correlation and t-test were employed in the study.  Correlation analysis reveals that a strong positive correlation between students’ beliefs about translation and their use of translation as learning strategy. T-test finds the significant difference in the use of translation as learning strategy between freshman and sophomore learner.

scholarly journals Who will benefit most from lymph node dissection in invasive upper tract urothelial carcinoma: a matched case-control study

2020 ◽  
Author(s):  
Zaishang Li ◽  
Xueying Li ◽  
Ying Liu ◽  
Kefeng Xiao
Keyword(s):  
Lymph Node ◽  
Urothelial Carcinoma ◽  
Lymph Node Dissection ◽  
Propensity Score Analysis ◽  
Upper Tract Urothelial Carcinoma ◽  
Node Dissection ◽  
Metastatic Tumors ◽  
Tumor Patients ◽  
Upper Tract ◽  
Significant Difference

Abstract Background: Limited or regional lymphadenectomy (lymph node dissection, LND), which can help in postoperative risk stratification, is performed with radical nephroureterectomy (RNU). However, the specific ones are appropriate for LND in upper tract urothelial carcinoma (UTUC) has long been debated. We determined the clinical value of LND in different groups of UTUC patients who underwent RNU and someone who benefit most from LND in this study.Methods: Patients diagnosed with UTUC between 2004 and 2015 with available TNM stage classification were identified from 18 Surveillance, Epidemiology and End Results (SEER) registries. Propensity score analysis was used to match the baseline differences of patients. Results: This study included 5426 patients, and 2000 patients with invasive UTUC were matched (1000 patients with LND vs. 1000 patients without LND). The 5-year cancer-specific survival (CSS) estimate for the total cohort was 73.5%. After matching, the LND group had a higher 5-year CSS rate than the no-LND group (77.2% vs. 70.1%, P = 0.002). In heterogeneity analysis, there were significant differences between LND groups and no-LND in stage T4 (52.5% vs. 23.3%, P< 0.001), lymph node metastatic tumors (48.0% vs. 21.2%, P=0.015) and non-distant metastatic tumors (78.5% vs. 71.5%, P = 0.002). According to the most recent pathological prognostic groups classification, significant differences in survival were found between groups only with stage IV tumors (52.0% vs. 25.1%, P< 0.001). However, only stage T4NxM0 tumor patients were found a significant difference between LND groups in subgroup analysis (57.4% vs. 29.9%, P< 0.001). Conclusions:LND may improve the survival of invasive UTUC patients who undergo RNU, especially in T4NxM0 tumor patients. However, this hypothesis requires further confirmation.

Patterns of lymphatic metastases in upper tract urothelial carcinoma: Experience from three comprehensive cancer centers.

2015 ◽  
Vol 33 (7_suppl) ◽  
pp. 349-349
Author(s):  
Surena F. Matin ◽  
Patrick Espiritu ◽  
Jonathan A. Coleman ◽  
John P. Sfakianos ◽  
Philippe E. Spiess
Keyword(s):  
Lymph Node ◽  
Urothelial Carcinoma ◽  
Renal Pelvis ◽  
Therapeutic Potential ◽  
Upper Tract Urothelial Carcinoma ◽  
Cancer Specific Survival ◽  
Cancer Centers ◽  
Upper Tract ◽  
Comprehensive Cancer Centers ◽  
Significant Difference

349 Background: Data on patterns of lymph node metastasis (LNM) for upper tract urothelial carcinoma (UTUC) is sparse despite its critical relevance for staging and therapeutic potential, with only 1 prior study detailing patterns of spread in 23 pathologically confirmed cases (Kondo T et al, Urol. 2007). Methods: We retrospectively identified and reviewed patients with pTany N+ M0 UTUC at 3 comprehensive cancer centers which routinely perform template-based dissections for UTUC. Anatomic locations of the primary tumor and positive lymph nodes based on the pathological specimens were analyzed, and maps of the location and incidence of LNM constructed. Results: A total of 92 patients with pN+ UTUC were identified, 63 of whom had renal pelvis (n=43) or proximal ureteral (n=20) tumors. LNM from left renal pelvis tumors occurred to the hilar (29.4%), pre- and para-aortic (50.0%) regions, with secondary spread to suprarenal (1.8%), interaortocaval (5.9%), and common iliac (2.9%) regions. LNM from right renal pelvic tumors occurred to the hilar (24.1%), pre- and para-caval (38.0%), retrocaval (13.8%), and inter-aortocaval (24.1%) areas. Left proximal ureteral tumors primarily had LNM to the hilar (46.1%), para-aortic (46.1%), and retro-aortic (7.7%) regions. Right proximal ureteral tumors had LNM to the hilar (36.4%), paracaval (54.5%), and retrocaval (9.1%) regions. A significant difference in survival was noted with patients who had organ confined tumor (p = 0.002) and a lymph node density of less than 25% (p = 0.005). The 1, 2, and 5-year cancer-specific survival rates of the overall cohort were 69%, 49%, and 27%, respectively. Conclusions: Our multi-institutional study provides data regarding the primary landing zones for LNM in renal pelvis and proximal ureter UTUC as well as guidance for developing anatomic dissection templates for prospective studies in UTUC incorporating surgical methodologies as a variable.

scholarly journals Characterization of Macrophages and TNF-α in Cleft Affected Lip Tissue

Cosmetics ◽  
2021 ◽  
Vol 8 (2) ◽  
pp. 42
Author(s):  
Jana Goida ◽  
Māra Pilmane
Keyword(s):  
Quantitative Method ◽  
Weak Correlation ◽  
Orofacial Clefts ◽  
Protective Mechanisms ◽  
Tissue Samples ◽  
Positive Correlation ◽  
Tnf Α ◽  
Significant Difference ◽  
Moderate Positive Correlation

Orofacial clefts are one of the most common congenital anomalies worldwide; however, morphopathogenesis of the clefts is not yet completely understood. Taking the importance of innate immunity into account, the aim of this work was to examine the appearance and distribution of macrophages (M) 1, M2, and TNF-α, as well as to deduce any possible intercorrelations between the three factors in cleft affected lip tissue samples. Twenty samples of soft tissue were collected from children during plastic surgery. Fourteen control tissue samples were obtained during labial frenectomy. Tissues were immunohistochemically stained, analysed by light microscopy using a semi-quantitative method, and the Mann–Whitney U and Spearman’s tests were used to evaluate statistical differences and correlations. A statistically significant difference in the distribution was observed only in regard to M1. A weak correlation was observed between M2 and TNF-α but a moderate one between M1 and M2 as well as M1 and TNF-α. However, only the correlation between M1 and M2 was statistically important. The rise in M1, alongside the positive correlation between M1 and TNF-α, suggested a more pro-inflammatory/inflammatory environment in the cleft affected lip tissue. The moderate positive correlation between M1 and M2 indicated an intensification of the protective mechanisms.

scholarly journals Correlation between ER, PR, P53, Ki67 expression, and high-risk HPV infection in patients with different levels of cervical intraepithelial neoplasia

2020 ◽  
Vol 18 ◽  
pp. 205873922093308
Author(s):  
Gao Yuan
Keyword(s):  
High Risk ◽  
Correlation Analysis ◽  
Cervical Intraepithelial Neoplasia ◽  
Intraepithelial Neoplasia ◽  
Hpv Infection ◽  
Ki67 Expression ◽  
Positive Correlation ◽  
Positive Rate ◽  
The Difference ◽  
High Risk Hpv

This study was designed to investigate the correlation between high-risk human papillomavirus (HPV) infection and the expression of IHC markers (ER, PR, p53, Ki67) in patients with different grades of cervical intraepithelial neoplasia (CIN). It was a retrospective study, which was conducted from June 2016 to June 2018. 140 specimens of CIN were collected from the pathology department of a certain hospital that included 40 specimens of CIN1, 50 specimens of CIN2 and 50 specimens of CIN3. The expression of ER, PR, P53 and Ki67 were determined by immunohistochemistry. The high-risk HPV infections were detected by PCR fluorescence quantification and were given the correlation analysis. In the 140 specimens, the positive rates of HPV16 and HPV18 in CIN1 specimens were 27.5% and 25.0% respectively, and in CIN2 specimens were 64.0% and 60.0% respectively, and in CIN3 specimens were 90.0% and 92.0% respectively, the difference were statistically significant (p<0.05). There were no significant correlation (p<0.05) between HPV16 and HPV18 positive rate and patient age, tissue differentiation, and tumor size. With the increased of CIN grade, the positive rate of ER, PR, P53 and Ki67 expression in specimen were also increased significantly, and the difference were statistically significant (p<0.05). Pearson correlation analysis showed there were positive correlation (p<0.05) between the positive rates of HPV16 and HPV18 and the positive rates of ER, PR, P53 and Ki67. With the increase of CIN level, the positive rates of high-risk HPV infection as well as ER, PR, P53 and Ki67 are increased, and they have positive correlation.

scholarly journals Comparison of Genomic Characterization in Upper Tract Urothelial Carcinoma and Urothelial Carcinoma of the Bladder

2021 ◽  
Author(s):  
Kaiwei Yang ◽  
Wei Yu ◽  
Huanhuan Liu ◽  
Feng Ding ◽  
Yanrui Zhang ◽  
...  
Keyword(s):  
Urothelial Carcinoma ◽  
Biological Diversity ◽  
Upper Tract Urothelial Carcinoma ◽  
Driver Gene ◽  
Driver Genes ◽  
Upper Tract ◽  
Significant Difference ◽  
Genomic Characterization ◽  
Carcinoma Of The Bladder ◽  
Subclonal Mutation

Abstract Background: Different genomic characterization in urothelial carcinoma (UC) by site of origin, may imply contrasting therapeutic opportunities and pathogenetic mechanisms. The aim of this study was to investigate whether the differences between upper tract urothelial carcinoma (UTUC) and urothelial carcinoma of the bladder (UCB) result from intrinsic biological diversity.Methods: We prospectively sequenced 118 tumors and matched blood DNA from Chinese UC patients using next-generation sequencing (NGS) techniques, including 45 UTUC and 73 UCB.Results: There were marked disparities in the mutational landscape for UC according to race and site of origin. Signature 22 for exposure to aristolochic acid (AA) and signature 10 for defects in polymerase POLE were only observed in the UTUC cohort. Conversely, signature 6 for defective DNA mismatch repair only existed in the UCB cohort. Compared to UCB, UTUC had higher clonal (p<0.001) and subclonal mutation numbers (p=0.015). TP53, PIK3CA, and FGFR3 mutations may be the driver genes for UTUC, whereas for UCB, the driver gene may be BRCA1. UTUC patients had lower PD-L1 than UCB patients. There was no significant difference in the number of DDR mutations, copy number variation (CNV) counts, tumor mutational burden (TMB) or clinical actionability between UTUC and UCB.Conclusions: UTUC and UCB exhibit significant differences in the prevalence of common genomic landscape and carcinogenesis. Consequently, molecular subtypes differ according to location, and these results may have important implications for the site-specific management of patients with urothelial carcinoma. Mutational signature may be used as a screening tool to assist clinical differential diagnosis between UTUC and UCB.

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