scholarly journals Development of a novel and rapid phenotype-based screening method to assess rice seedling growth

Plant Methods ◽  
2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Lena Vlaminck ◽  
Chananchida Sang-Aram ◽  
Deborah Botterman ◽  
Christine Jewel C. Uy ◽  
Mary Kay Harper ◽  
...  
Keyword(s):  
Plant Growth ◽  
Seedling Growth ◽  
Growth Regulation ◽  
Marine Invertebrates ◽  
Screening Method ◽  
Rice Seedling ◽  
Plant Growth Promoting Bacteria ◽  
Stress Tests ◽  
Wide Range ◽  
The Impact

Abstract Background Rice (Oryza sativa) is one of the most important model crops in plant research. Despite its considerable advantages, (phenotypic) bioassays for rice are not as well developed as for Arabidopsis thaliana. Here, we present a phenotype-based screening method to study shoot-related parameters of rice seedlings via an automated computer analysis. Results The phenotype-based screening method was validated by testing several compounds in pharmacological experiments that interfered with hormone homeostasis, confirming that the assay was consistent with regard to the anticipated plant growth regulation and revealing the robustness of the set-up in terms of reproducibility. Moreover, abiotic stress tests using NaCl and DCMU, an electron transport blocker during the light dependent reactions of photosynthesis, confirmed the validity of the new method for a wide range of applications. Next, this method was used to screen the impact of semi-purified fractions of marine invertebrates on the initial stages of rice seedling growth. Certain fractions clearly stimulated growth, whereas others inhibited it, especially in the root, illustrating the possible applications of this novel, robust, and fast phenotype-based screening method for rice. Conclusions The validated phenotype-based and cost-efficient screening method allows a quick and proper analysis of shoot growth and requires only small volumes of compounds and media. As a result, this method could potentially be used for a whole range of applications, ranging from discovery of novel biostimulants, plant growth regulators, and plant growth-promoting bacteria to analysis of CRISPR knockouts, molecular plant breeding, genome-wide association, and phytotoxicity studies. The assay system described here can contribute to a better understanding of plant development in general.

Harvesting the complex pathways of antibiotic production and resistance of soil bacilli for optimizing plant microbiome

2020 ◽  
Vol 96 (9) ◽  
Author(s):  
Qihui Hou ◽  
Ilana Kolodkin-Gal
Keyword(s):  
Plant Growth ◽  
Antibiotic Production ◽  
Resistance Mechanisms ◽  
Plant Growth Promoting Bacteria ◽  
Plant Defences ◽  
Wide Range ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Rhizosphere Environment ◽  
Plant Microbiome

ABSTRACT A sustainable future increasing depends on our capacity to utilize beneficial plant microbiomes to meet our growing needs. Plant microbiome symbiosis is a hallmark of the beneficial interactions between bacteria and their host. Specifically, colonization of plant roots by biocontrol agents and plant growth-promoting bacteria can play an important role in maintaining the optimal rhizosphere environment, supporting plant growth and promoting its fitness. Rhizosphere communities confer immunity against a wide range of foliar diseases by secreting antibiotics and activating plant defences. At the same time, the rhizosphere is a highly competitive niche, with multiple microbial species competing for space and resources, engaged in an arms race involving the production of a vast array of antibiotics and utilization of a variety of antibiotic resistance mechanisms. Therefore, elucidating the mechanisms that govern antibiotic production and resistance in the rhizosphere is of great significance for designing beneficial communities with enhanced biocontrol properties. In this review, we used Bacillus subtilis and B. amyloliquefaciens as models to investigate the genetics of antibiosis and the potential for its translation of into improved plant microbiome performance.

scholarly journals Characterization and potential of plant growth-promoting rhizobacteria on rice seedling growth and the effect on Xanthomonas oryzae pv. oryzae

2019 ◽  
Vol 20 (12) ◽  
Author(s):  
Haliatur Rahma ◽  
NURBAILIS ◽  
NILA KRISTINA
Keyword(s):  
Plant Growth ◽  
Seedling Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Rice Seedling ◽  
Xanthomonas Oryzae ◽  
Resistant Variety ◽  
Dual Culture ◽  
Rice Seedlings ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract. Rahma H, Nurbailis, Kristina N. 2019. Characterization and potential of plant growth-promoting rhizobacteria on rice seedling growth and the effect on Xanthomonas oryzae pv. oryzae. Biodiversitas 20: 3654-3661. Xanthomonas oryzae pv. oryzae (Xoo), a major limiting factor in rice production, and the use of resistant Xoo varieties have failed to control the bacterial pathogens as well as increased yield. It is due to the diversity in pathotypes, caused by environmental factors, the nature of resistant variety used, and gene mutation. The aims of this study were to select rhizobacterial strains with the potential of suppressing Xoo growth and promoting the growth of rice seedlings. This experiment was conducted in a completely randomized design (CRD) using seven rhizobacterial isolates selected through a dual culture test, with four replications. There were four isolates that potential in inhibiting the growth of Xoo, namely KJKB5.4, LMTSA5.4, Bacillus cereus AJ34, and Alcaligenes faecalis AJ14, with inhibition diameters greater than 11.50 mm. Rhizobacterial supernatant of 4 potential isolates has a zone of inhibition ranging from 12.25 to 24.00 mm. Four potential isolates were also able to solubilize phosphate, produce indole acetic acid (IAA) growth hormone, and siderophore, as well as enhance the growth of rice seedlings. Based on the nucleic acid sequencing of LMTSA5.4, KJKB5.4, and RK12 isolates were identified as Stenotrophomonas malthopilia strain LMG 958 (99.13%) accession NR 119220.1, Stenotrophomonas pavanii strain LMG 25348 (95.84%) accession NR 118008.1 and Ochrobactrum ciceri strain ca-34 (92.91%) accession NR115819.1.

scholarly journals Toxicity of seed-applied pesticides to Azospirillum spp.: an approach based on bacterial count in the maize rhizosphere

2020 ◽  
Vol 48 (2) ◽  
pp. 241-246 ◽  
Author(s):  
Lucas Caiubi Pereira ◽  
Cristiane de Carvalho ◽  
Andreia Kazumi Suzukawa ◽  
Larissa Vinis Correia ◽  
Renata Cristiane Pereira ◽  
...  
Keyword(s):  
Plant Growth ◽  
Bacterial Count ◽  
Plant Growth Promoting Bacteria ◽  
Maize Rhizosphere ◽  
Seed Surface ◽  
Maize Seeds ◽  
Cell Mortality ◽  
Plant Growth Promoting ◽  
Root Tissues ◽  
The Impact

The objective of this work was to investigate the impact of pesticides on the survival of the plant growth- promoting bacteria Azospirillum in the surface of coated seeds as well as in the maize rhizosphere. Our results showed that the greater the time that the bacteria are in contact with the seed surface, the higher the cell mortality. In uncoated maize seeds, inoculation increased bacterial concentration in root tissues and ensured superior plant growth up to 12 hours of Azospirillum contact with the seed surface, while for the coated seeds, a similar performance was observed only up to six hours of exposure.

The Effect of Zinc Oxide Nanoparticles (ZnO NPs) on Vigna mungo L. Seedling Growth and Antioxidant Activity

2020 ◽  
Vol 10 (2) ◽  
pp. 117-122
Author(s):  
Kantabathini Venkata Pavani ◽  
Mallula Beulah ◽  
Govinda Udayar Sai Poojitha
Keyword(s):  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Glutathione Reductase ◽  
Seedling Growth ◽  
Vigna Mungo ◽  
Guaiacol Peroxidase ◽  
Zno Nps ◽  
Wide Range ◽  
The Impact

Aim: The purpose of this study was to test the phytotoxicity effect of ZnONPs on Vigna mungo L. seedling growth and antioxidant activity. Methods: Vigna mungo L. Seeds were treated with to a wide range of ZnO NPs ranging 5 to25mg/100ml for 8hours. Vigna mungo seeds that were soaked in ZnO NPs solution were sown in pots (20 cm × 40 cm) filled with red soil and a layer of coco peat. The effect of ZnO NPs on morphological, biochemical and antioxidant activity in Vigna mungo L. plants was investigated after 15,30,45 and 60 days. Results: The impact of ZnO NPs on plant growth characteristics and biochemical changes in Vigna mungo L. plants was investigated after 15,30,45 and 60 days. The ZnONPs exposure significantly enhanced germination percentage by 111.3% but root length (75.25%), shoot length (89.81%), number of leaves (91.66%), length of leaves (76%), width of leaves (67.27%), fresh weight of plant (27.96%) and dry weight of plant (28.23%) decreased in the treated plants after 60 days exposure to 25mg/100ml compared to the untreated control. Interestingly, treated plants after 60 days exposure to 25mg/100ml increased significantly the chlorophyll (115.0%), reducing sugars (244.4%), total sugars (212.72%) protein (181.8%). Treatment to Vigna mungo L. seeds with ZnONPs has been found to induce the activities of antioxidant enzymes such as Guaiacol peroxidase, Glutathione Reductase, Catalase and increase in the ascorbic acid and hydrogen peroxide contents. TEM images revealed that the aggregated ZnO NPs to be deposited inside the seed. Conclusion: Vigna mungo seeds treated with different concentrations of ZnO NPs showed decreased root growth and increased germination index, shoot and leaf growth. There was a significant change in Glutathione reductase, Guaiacol peroxidase and Catalase activity and ascorbic acid and hydrogen peroxide of Vigna mungo exposed to ZnONPs. Aggregated nanoparticles penetration into the intracellular region of the seed was observed.A complete study on the toxic effects of ZnO NPs can help significantly in the safe disposal of ENPs for the reduction of adverse effects in both environmental and agricultural systems.

Plant Growth Regulation Activity of Column Chromatography Fractions from Mikania micrantha H.B.K. on Raphanus sativus

2013 ◽  
Vol 726-731 ◽  
pp. 4397-4400
Author(s):  
Yan Zhao ◽  
Min Ye ◽  
Li Ming Fan ◽  
You Gui Zha ◽  
Xiao Yu Gao
Keyword(s):  
Plant Growth ◽  
Seedling Growth ◽  
Raphanus Sativus ◽  
Growth Regulation ◽  
Chemical Constituents ◽  
Chemical Compounds ◽  
Strong Activity ◽  
Mikania Micrantha ◽  
Plant Growth Regulation ◽  
Seeds Germination

Some chemical compounds in low polar fractions ofMikania micranthaH.B.K. extracts can strongly influence seeds germination and seedling growth ofRaphanus sativus. In this study, we provide evidence that the chemical compounds in a low polar fraction chromatographed on normal phase silica gel columns from petroleum ether extract ofM. micranthahave strong activity to inhibit the seeds germination and seedling growth ofRaphanus sativus. Two main chemical constituents, α-Bisabolol and Eudesma-5,11(13)-dien-8,12-olide, in the active fraction were confirmed by GC-MS analysis. Key words:Mikania micranthaH.B.K.,Raphanus sativus, plant growth regulation activity, fraction, GC-MS

scholarly journals The Contrivance of Plant Growth Promoting Microbes to Mitigate Climate Change Impact in Agriculture

2021 ◽  
Vol 9 (9) ◽  
pp. 1841
Author(s):  
Angelika Fiodor ◽  
Surender Singh ◽  
Kumar Pranaw
Keyword(s):  
Climate Change ◽  
Plant Growth ◽  
Global Climate ◽  
Acc Deaminase ◽  
Crop Productivity ◽  
Plant Growth Promoting Bacteria ◽  
Hormone Synthesis ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
The Impact

Combating the consequences of climate change is extremely important and critical in the context of feeding the world’s population. Crop simulation models have been extensively studied recently to investigate the impact of climate change on agricultural productivity and food security. Drought and salinity are major environmental stresses that cause changes in the physiological, biochemical, and molecular processes in plants, resulting in significant crop productivity losses. Excessive use of chemicals has become a severe threat to human health and the environment. The use of beneficial microorganisms is an environmentally friendly method of increasing crop yield under environmental stress conditions. These microbes enhance plant growth through various mechanisms such as production of hormones, ACC deaminase, VOCs and EPS, and modulate hormone synthesis and other metabolites in plants. This review aims to decipher the effect of plant growth promoting bacteria (PGPB) on plant health under abiotic soil stresses associated with global climate change (viz., drought and salinity). The application of stress-resistant PGPB may not only help in the combating the effects of abiotic stressors, but also lead to mitigation of climate change. More thorough molecular level studies are needed in the future to assess their cumulative influence on plant development.

scholarly journals Recent Developments in the Study of Plant Microbiomes

2021 ◽  
Vol 9 (7) ◽  
pp. 1533
Author(s):  
Bernard R. Glick ◽  
Elisa Gamalero
Keyword(s):  
Plant Growth ◽  
Cross Sections ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Strains ◽  
Bacterial Consortia ◽  
Positive Effects ◽  
Wide Range ◽  
Recent Developments ◽  
Plant Growth Promoting ◽  
Bacteria And Fungi

To date, an understanding of how plant growth-promoting bacteria facilitate plant growth has been primarily based on studies of individual bacteria interacting with plants under different conditions. More recently, it has become clear that specific soil microorganisms interact with one another in consortia with the collective being responsible for the positive effects on plant growth. Different plants attract different cross-sections of the bacteria and fungi in the soil, initially based on the composition of the unique root exudates from each plant. Thus, plants mostly attract those microorganisms that are beneficial to plants and exclude those that are potentially pathogenic. Beneficial bacterial consortia not only help to promote plant growth, these consortia also protect plants from a wide range of direct and indirect environmental stresses. Moreover, it is currently possible to engineer plant seeds to contain desired bacterial strains and thereby benefit the next generation of plants. In this way, it may no longer be necessary to deliver beneficial microbiota to each individual growing plant. As we develop a better understanding of beneficial bacterial microbiomes, it may become possible to develop synthetic microbiomes where compatible bacteria work together to facilitate plant growth under a wide range of natural conditions.

Sign in / Sign up

Export Citation Format

Share Document