IRS-1 gene polymorphism and DNA damage in pregnant women with diabetes or mild gestational hyperglycemia

Background. Reference symphysis-fundal height (SFH) curves for pregnancies complicated by maternal hyperglycemia are not available. Objective. To build an SFH curve according to gestational age for pregnant women with hyperglycemia-type 2 diabetes (T2DM), gestational diabetes mellitus (GDM), or mild gestational hyperglycemia (MGH) and compare it with three other curves in use in Brazil. Methods. Prospective cohort study of 422 pregnant women with hyperglycemia attending the Perinatal Diabetes Research Center (PDRC) of Botucatu Medical School, São Paulo State University/UNESP. Between 13 and 41 weeks of pregnancy, 2470 SFH measurements were obtained (mean 5.85 per woman). For the assessment of glycemic control, 2074 glucose level measurements were taken and the glycemic mean (GM) at each gestational week was estimated. Results. GM was adequate (<120 mg/dL) in 94.9% and inadequate (≥120 mg/dL) in 5.1% of the cases. The equation applied for SFH prediction was expressed as SFH=1.082+0.966∗week (r2=84.6%). At visual analysis, P10 and P90 SFH measurements were higher in the study curve than in the three other curves. Statistical analysis confirmed that SFH median values in this study were higher than those in the reference curve of habitual risk pregnancies, especially after 19 weeks of pregnancy. Conclusion. Taking into account that the maternal hyperglycemia was at strict control, our unedited results suggest that the current SFH curve can be a useful tool in prenatal care of T2DM, GDM, and MGH pregnant women.

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274: Association of the Pro12Ala polymorphism of PPARγ2 with mild gestational hyperglycemia and gestational diabetes

American Journal of Obstetrics and Gynecology ◽

10.1016/j.ajog.2012.10.439 ◽

2013 ◽

Vol 208 (1) ◽

pp. S125 ◽

Cited By ~ 1

Author(s):

Kristin Taylor ◽

Paula Lima ◽

Rafael Gelaleti ◽

Mario Hirata ◽

Yuri Sinzato ◽

...

Keyword(s):

Gestational Diabetes ◽

Pro12ala Polymorphism ◽

Mild Gestational Hyperglycemia ◽

Gestational Hyperglycemia

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Influence of vitamin D binding protein polymorphism, demographics and lifestyle factors on vitamin D status of healthy Malaysian pregnant women

10.21203/rs.3.rs-27917/v3 ◽

2020 ◽

Author(s):

Siew-Siew Lee ◽

King-Hwa Ling ◽

Maiza Tusimin ◽

Raman Subramaniam ◽

Kartini Farah Rahim ◽

...

Keyword(s):

Vitamin D ◽

Gene Polymorphism ◽

Pregnant Women ◽

Binding Protein ◽

Lifestyle Factors ◽

Vitamin D Supplementation ◽

Dietary Vitamin ◽

Vitamin D Binding Protein ◽

Significant Positive Association ◽

Cross Sectional

Abstract Background: Vitamin D deficiency (VDD) has been related to vitamin D binding protein (GC) gene polymorphism, demographics and lifestyle factors in different populations. However, previous studies only focused on demographic and lifestyle factors or genetic factors alone. Therefore, this cross-sectional study aimed to assess the association between GC gene polymorphism, demographics and lifestyle factors with VDD among Malaysian pregnant women. Method: Information on demographic characteristics, dietary vitamin D intake from supplement and food, time spent outdoors, skin type and clothing were collected using questionnaire. Plasma total 25-hydroxyvitamin D (25OHD) levels were measured using a Ultra-High-Performance Chromatography (UHPLC). Maternal GC single nucleotide polymorphisms (SNPs) (rs4588 and rs7041) were determined using restriction fragment length polymorphism (RFLP) technique. Results: Results showed that 50.2% of pregnant women were vitamin D deficient (25OHD <30 nmol/L). VDD (25OHD <30 nmol/L) was significantly associated with age, veiled clothing, maternal vitamin D intakes, from both food and supplements, and GC rs7041(and GC diplotypes). In contrast to previous studies that reported for non-pregnant population, significant positive association was found between CC genotype for SNP GC rs7041, GC 1s-1s and GC If-2 with risk of VDD (25OHD <30 nmol/L). Conclusions: The high prevalence of maternal VDD found in this study suggests the need for urgent development and implementation of vitamin D supplementation or fortification strategies to reduce VDD among pregnant women. The discrepancy in the association between GC rs7041 gene polymorphism and VDD reflects the variation in the factors associated with VDD in pregnancy compared to non-pregnant state

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Pregnancy Induces Transcriptional Activation of the Peripheral Innate Immune System and Increases Oxidative DNA Damage among Healthy Third Trimester Pregnant Women

PLoS ONE ◽

10.1371/journal.pone.0046736 ◽

2012 ◽

Vol 7 (11) ◽

pp. e46736 ◽

Cited By ~ 5

Author(s):

Xinyin Jiang ◽

Haim Y. Bar ◽

Jian Yan ◽

Allyson A. West ◽

Cydne A. Perry ◽

...

Keyword(s):

Dna Damage ◽

Immune System ◽

Pregnant Women ◽

Transcriptional Activation ◽

Innate Immune System ◽

Oxidative Dna Damage ◽

Innate Immune ◽

Third Trimester

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DNA damage analysis concerning GSTM1 and GSTT1 gene polymorphism in gold jewellery workers from Peshawar Pakistan

Biomarkers ◽

10.1080/1354750x.2020.1791253 ◽

2020 ◽

Vol 25 (6) ◽

pp. 483-489

Author(s):

Muhammad Khisroon ◽

Ajmal Khan ◽

Asma Ayub ◽

Ihsan Ullah ◽

Javeed Farooqi ◽

...

Keyword(s):

Dna Damage ◽

Gene Polymorphism ◽

Damage Analysis ◽

Gstt1 Gene ◽

Gold Jewellery

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Association study of rs1801282 PPARG gene polymorphism and immune cells and cytokine levels in a Spanish pregnant women cohort and their offspring

Journal of Biomedical Science ◽

10.1186/s12929-020-00694-3 ◽

2020 ◽

Vol 27 (1) ◽

Author(s):

Maria García-Ricobaraza ◽

Mercedes García-Bermúdez ◽

Francisco J. Torres-Espinola ◽

M. Teresa Segura Moreno ◽

Mathieu N. Bleyere ◽

...

Keyword(s):

Immune System ◽

Gene Polymorphism ◽

Cord Blood ◽

Pregnant Women ◽

Venous Blood ◽

Innate Immune ◽

Peroxisome Proliferator ◽

Blood Samples ◽

Inflammatory Reactions ◽

Immune Parameters

Abstract Background Peroxisome proliferator activated receptor gamma (PPARG) belongs to the nuclear receptor superfamily functioning as transcription factors to regulate cellular differentiation, development and metabolism. Moreover, it has been implicated in the regulation of lipid metabolism, as well as the maturation of monocytes/macrophages and the control of inflammatory reactions. The aim of this study was to evaluate the relationship between the Pro12Ala (rs1808212) PPARG gene polymorphism on immune molecular and cellular components in mothers and their offspring participating in the PREOBE study. Methods DNA from maternal venous blood samples at 24, 34 and 40 gestational weeks, plus cord blood samples was extracted. Pro12Ala PPARG polymorphism genotyping was performed, and immune system markers were analyzed by flow cytometry. Results Study findings revealed no effect of rs1808212 PPARG genotypes on innate immune parameters in mothers and their offspring; however, CD4 + /CD8 + ratio were decreased at 24 and 34 weeks in pregnant women carrying the CG (Pro12Ala) rs1808212 polymorphism, (p = 0,012 and p = 0,030; respectively). Only CD19 levels in peripheral blood were significantly higher at delivery in pregnant women carrying the CC (Pro12Pro) genotype (p ≤ 0.001). Moreover, there were statistically significant differences in leukocytes and neutrophils maternal levels at 34 weeks of gestation, being lower in carriers of Pro12Ala genotype (p = 0.028 and p = 0.031, respectively). Conclusions Results suggest that Pro12Ala PPARG polymorphism may have an effect on some cell and immune parameters in pregnant women during pregnancy and at time of delivery. However, newborn innate immune system does not seems to be influenced by PPARG Pro12Ala polymorphism in cord blood.

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