scholarly journals Genomic adaptations of Campylobacter jejuni to long-term human colonization

Gut Pathogens ◽  
2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Samuel J. Bloomfield ◽  
Anne C. Midwinter ◽  
Patrick J. Biggs ◽  
Nigel P. French ◽  
Jonathan C. Marshall ◽  
...  
Keyword(s):  
Signal Transduction ◽  
New Zealand ◽  
Gastrointestinal Tract ◽  
16S Rrna ◽  
Cell Motility ◽  
Campylobacter Jejuni ◽  
Nucleotide Polymorphisms ◽  
Biochemical Tests ◽  
Human Colonization

Abstract Background Campylobacter is a genus of bacteria that has been isolated from the gastrointestinal tract of humans and animals, and the environments they inhabit around the world. Campylobacter adapt to new environments by changes in their gene content and expression, but little is known about how they adapt to long-term human colonization. In this study, the genomes of 31 isolates from a New Zealand patient and 22 isolates from a United Kingdom patient belonging to Campylobacter jejuni sequence type 45 (ST45) were compared with 209 ST45 genomes from other sources to identify the mechanisms by which Campylobacter adapts to long-term human colonization. In addition, the New Zealand patient had their microbiota investigated using 16S rRNA metabarcoding, and their level of inflammation and immunosuppression analyzed using biochemical tests, to determine how Campylobacter adapts to a changing gastrointestinal tract. Results There was some evidence that long-term colonization led to genome degradation, but more evidence that Campylobacter adapted through the accumulation of non-synonymous single nucleotide polymorphisms (SNPs) and frameshifts in genes involved in cell motility, signal transduction and the major outer membrane protein (MOMP). The New Zealand patient also displayed considerable variation in their microbiome, inflammation and immunosuppression over five months, and the Campylobacter collected from this patient could be divided into two subpopulations, the proportion of which correlated with the amount of gastrointestinal inflammation. Conclusions This study demonstrates how genomics, phylogenetics, 16S rRNA metabarcoding and biochemical markers can provide insight into how Campylobacter adapts to changing environments within human hosts. This study also demonstrates that long-term human colonization selects for changes in Campylobacter genes involved in cell motility, signal transduction and the MOMP; and that genetically distinct subpopulations of Campylobacter evolve to adapt to the changing gastrointestinal environment.

Plant mortality and seedling recruitment responses to flupropanate in grassland populations of Nassella trichotoma

2017 ◽  
Vol 70 ◽  
pp. 160-164 ◽  
Author(s):  
G.W. Bourdôt ◽  
S. Jackman ◽  
D.J. Saville
Keyword(s):  
New Zealand ◽  
Dose Response ◽  
Sodium Salt ◽  
Seedling Recruitment ◽  
Residual Activity ◽  
Application Rate ◽  
Plant Mortality ◽  
Complete Control ◽  
Bio Synthesis

Flupropanate (sodium 2,2,3,3 tetrafluoropropanate), a slow-acting lipid bio- synthesis-inhibiting herbicide, was recently registered in New Zealand as Taskforce (745 g/L flupropanate as the sodium salt) for the selective and long-term control of Nassella trichotoma (nassella tussock) in pastures. In five dose-response experiments in permanent hill pastures in Canterbury, conducted between 2012 and 2016, we measured the efficacy of the herbicide against established plants of N. trichotoma and its residual activity against recruiting seedlings. Mortality, as an average across the five sites, was 93% 1.5 years after applying 1.49 kg flupropanate/ha (the label-recommended rate), and 100% at 2.98 kg/ha. This indicates that an application rate higher than the label rate will be necessary for complete control of a N. trichotoma infestation. The presence of 1,000 and 6,250 visible seedlings of N. trichotoma/ha in the autumn 3.2 and 2.1 years after applying 1.49 kg flupropanate/ha (at a Greta Valley and Scargill site respectively) indicates that the herbicide’s soil residues had decayed within 12 months to a concentration lower than necessary to kill the germinating seedlings of N. trichotoma.

Co-Design and Open Trial of Starship Rescue, a Cognitive Behavior Therapy, Biofeedback, and Game-Based eHealth Intervention to Treat Anxiety in Children and Young People with Long-Term Physical Conditions (Preprint)

2020 ◽  
Author(s):  
Hiran Thabrew ◽  
Karolina Stasiak ◽  
Harshali Kumar ◽  
Tarique Naseem ◽  
Christopher Frampton ◽  
...  
Keyword(s):  
Quality Of Life ◽  
New Zealand ◽  
Young People ◽  
Preliminary Evidence ◽  
Children And Young People ◽  
Physical Conditions ◽  
System Usability Scale ◽  
Ehealth Intervention

BACKGROUND Approximately 10% to 12% of New Zealand children and young people have long-term physical conditions (also known as chronic illnesses) and are more likely to develop psychological problems, particularly anxiety and depression. Delayed treatment leads to worse physical and mental healthcare, school absence, and poorer long-term outcomes. Recently, electronic health (eHealth) interventions, especially those based on the principles of Cognitive Behavior Therapy (CBT), have been shown to be as good as face-to-face therapy. Biofeedback techniques have also been shown to enhance relaxation during the treatment of anxiety. However, these modalities have rarely been combined. Young people with long-term physical conditions have expressed a preference for well-designed and technologically-based support to deal with psychological issues, especially anxiety. OBJECTIVE This study aimed to co-design and evaluate the (i) acceptability and (ii) usability of a CBT and biofeedback-based, 5-module eHealth game called ‘Starship Rescue’ and (iii) to provide preliminary evidence regarding its effectiveness in addressing anxiety and quality of life in young people with long-term physical conditions. METHODS Starship Rescue was co-designed with children and young people from a tertiary hospital in Auckland, New Zealand. Following this, 24 young people aged 10 to 17 years were enrolled in an open trial, during which they were asked to use the game for an 8-week period. Acceptability of the game to all participants was assessed using a brief, open-ended questionnaire, and more detailed feedback was obtained from a subset of 10 participants via semi-structured interviews. Usability was evaluated via the System Usability Scale (SUS) and device-recorded frequency and duration of access on completion of the game. Anxiety levels were measured prior to commencement, on completion of the game, and 3 months later using the Generalized Anxiety Disorder 7-item scale (GAD-7) and Spence Child Anxiety Scales (SCAS), and at the start of each module and at the end of the game using an embedded Likert/visual analog scale. Quality of life was measured prior to commencement and on completion of the game using the Pediatric Quality of Life Scale (PEDS-QL). RESULTS Users gave Starship Rescue an overall rating of 5.9 out of 10 (range 3-10 and a mean score of 71 out of 100 (SD 11.7; min 47.5; max 90) on the System Usability Scale (SUS). The mean time period for use of the game was just over 11-weeks (78.8 days, 13.5 hours, 40 minutes). Significant reductions in anxiety were noted between the start and end of the game on the GAD-7 (-4.6 (p=0.000)), SCAS (-9.6 (p=0.005)), and the Likert/visual analogue scales (-2.4 (p=0.001)). Quality of life also improved on the PedsQL scale (+4.3 (p=0.042)). All changes were sustained at 3-month follow-up. CONCLUSIONS This study provides preliminary evidence for Starship Rescue being an acceptable, usable and effective eHealth intervention for addressing anxiety in young people with long-term physical conditions. Further evaluation is planned via a more formal randomized controlled trial. CLINICALTRIAL Australian New Zealand Clinical Trials Network Registry (ANZCTR): ACTRN12616001253493p;https://www.anzctr.org.au/Trial/Registration/TrialReview.aspx?id=371443 (Archived by WebCite at http://www.webcitation.org/6sYB716lf)

scholarly journals A long-term survivor of clear cell sarcoma-like tumor of the gastrointestinal tract with liver metastasis: a case report

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Takuhisa Okada ◽  
Yasumitsu Hirano ◽  
Shintaro Ishikawa ◽  
Hiroka Kondo ◽  
Toshimasa Ishii ◽  
...  
Keyword(s):  
Small Intestine ◽  
Lymph Node ◽  
Gastrointestinal Tract ◽  
Liver Metastasis ◽  
Surgical Resection ◽  
Clear Cell ◽  
Clear Cell Sarcoma ◽  
Cell Sarcoma

Abstract Background Clear cell sarcoma-like tumor of the gastrointestinal tract (CCSLTGT) is extremely rare. It is a mesenchymal neoplasm that usually forms in the small intestine of adolescents and young adults, is prone to local recurrence and metastasis, and has a high mortality rate. We report a patient with CCSLTGT with lymph node- and liver metastases, who continues to survive 6 years after initial surgical resection. Case presentation A 38-year-old woman presented with lightheadedness. Laboratory analysis revealed anemia (hemoglobin, 6.7 g/dL), and enhanced computed tomography (CT) demonstrated a mass in the small intestine, about 6 cm in diameter, with swelling of 2 regional lymph nodes. Double-balloon small intestine endoscopic examination revealed a tumor accompanied by an ulcer; the biopsy findings suggested a primary cancer of the small intestine. She was admitted, and we then performed a laparotomy for partial resection of the small intestine with lymph node dissection. Pathologic examination revealed CCSLTGT with regional lymph node metastases. About 3 years later, follow-up CT revealed a single liver metastasis. Consequently, she underwent a laparoscopic partial liver resection. Histopathologic examination confirmed that the liver metastasis was consistent with CCSLTGT. It has now been 3 years without a recurrence. Conclusion Repeated radical surgical resection with close follow-up may be the only way to achieve long-term survival in patients with CCLSTGT.

scholarly journals Locking down the Impact of New Zealand’s COVID-19 Alert Level Changes on Pets

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 758
Author(s):  
Fiona Esam ◽  
Rachel Forrest ◽  
Natalie Waran
Keyword(s):  
New Zealand ◽  
Separation Anxiety ◽  
National Surveys ◽  
Normal Life ◽  
Pet Owners ◽  
Level 1 ◽  
The Impact ◽  
Better Than

The influence of the COVID-19 pandemic on human-pet interactions within New Zealand, particularly during lockdown, was investigated via two national surveys. In Survey 1, pet owners (n = 686) responded during the final week of the five-week Alert Level 4 lockdown (highest level of restrictions—April 2020), and survey 2 involved 498 respondents during July 2020 whilst at Alert Level 1 (lowest level of restrictions). During the lockdown, 54.7% of owners felt that their pets’ wellbeing was better than usual, while only 7.4% felt that it was worse. Most respondents (84.0%) could list at least one benefit of lockdown for their pets, and they noted pets were engaged with more play (61.7%) and exercise (49.7%) than pre-lockdown. Many respondents (40.3%) expressed that they were concerned about their pet’s wellbeing after lockdown, with pets missing company/attention and separation anxiety being major themes. In Survey 2, 27.9% of respondents reported that they continued to engage in increased rates of play with their pets after lockdown, however, the higher levels of pet exercise were not maintained. Just over one-third (35.9%) of owners took steps to prepare their pets to transition out of lockdown. The results indicate that pets may have enjoyed improved welfare during lockdown due to the possibility of increased human-pet interaction. The steps taken by owners to prepare animals for a return to normal life may enhance pet wellbeing long-term if maintained.

scholarly journals Cumulative incidence and risk factors for radiation induced leukoencephalopathy in high grade glioma long term survivors

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Robert Terziev ◽  
Dimitri Psimaras ◽  
Yannick Marie ◽  
Loic Feuvret ◽  
Giulia Berzero ◽  
...  
Keyword(s):  
Risk Factors ◽  
Cumulative Incidence ◽  
High Grade Glioma ◽  
High Grade ◽  
Nucleotide Polymorphisms ◽  
Increased Risk ◽  
Radiation Induced ◽  
Long Term Survivors

AbstractThe incidence and risk factors associated with radiation-induced leukoencephalopathy (RIL) in long-term survivors of high-grade glioma (HGG) are still poorly investigated. We performed a retrospective research in our institutional database for patients with supratentorial HGG treated with focal radiotherapy, having a progression-free overall survival > 30 months and available germline DNA. We reviewed MRI scans for signs of leukoencephalopathy on T2/FLAIR sequences, and medical records for information on cerebrovascular risk factors and neurological symptoms. We investigated a panel of candidate single nucleotide polymorphisms (SNPs) to assess genetic risk. Eighty-one HGG patients (18 grade IV and 63 grade III, 50M/31F) were included in the study. The median age at the time of radiotherapy was 48 years old (range 18–69). The median follow-up after the completion of radiotherapy was 79 months. A total of 44 patients (44/81, 54.3%) developed RIL during follow-up. Twenty-nine of the 44 patients developed consistent symptoms such as subcortical dementia (n = 28), gait disturbances (n = 12), and urinary incontinence (n = 9). The cumulative incidence of RIL was 21% at 12 months, 42% at 36 months, and 48% at 60 months. Age > 60 years, smoking, and the germline SNP rs2120825 (PPARg locus) were associated with an increased risk of RIL. Our study identified potential risk factors for the development of RIL (age, smoking, and the germline SNP rs2120825) and established the rationale for testing PPARg agonists in the prevention and management of late-delayed radiation-induced neurotoxicity.

scholarly journals Uncommon Non-Candida Yeasts in Healthy Turkeys—Antimicrobial Susceptibility and Biochemical Characteristic of Trichosporon Isolates

Pathogens ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 538
Author(s):  
Kamila Bobrek ◽  
Ireneusz Sokół ◽  
Andrzej Gaweł
Keyword(s):  
Gastrointestinal Tract ◽  
Amphotericin B ◽  
Antimicrobial Susceptibility ◽  
Antifungal Susceptibility ◽  
Range Limit ◽  
Biochemical Tests ◽  
Lower Range ◽  
Normal Microflora ◽  
Severe Infections ◽  
Diverse Community

The microbiota of the gastrointestinal tract of humans and animals is inhabited by a diverse community of bacteria, fungi, protozoa, and viruses. In cases where there is an imbalance in the normal microflora or an immunosuppression on the part of the host, these opportunistic microorganisms can cause severe infections. The study presented here evaluates the biochemical and antifungal susceptibility features of Trichosporon spp., uncommon non-Candida strains isolated from the gastrointestinal tract of healthy turkeys. The Trichosporon coremiiforme and Trichosporon (Apiotrichum) montevideense accounted for 7.7% of all fungi isolates. The biochemical tests showed that Trichosporon coremiiforme had active esterase (C4), esterase-lipase (C8) valine arylamidase, naphthol-AS-BI phosphohydrolase, α-galactosidase, and β-glucosidase. Likewise, Trichosporon montevideense demonstrated esterase-lipase (C8), lipase (C14), valine arylamidase, naphthol-AS-BI phosphohydrolase, α-galactosidase, and β-glucosidase activity. T.coremiiforme and T. monteviidense isolated from turkeys were itraconazole resistant and amphotericin B, fluconazole, and voriconazole susceptible. Compared with human isolates, the MIC range and MIC values of turkey isolates to itraconazole were in a higher range limit in both species, while MIC values to amphotericin B, fluconazole, and voriconazole were in a lower range limit. Furthermore, the obtained ITS1—5.8rRNA—ITS2 fragment sequences were identical with T. coremiiforme and T. montevideense sequences isolated from humans indicating that these isolates are shared pathogens.

The New Chinese Community in New Zealand: Local Outcomes of Transnationalism

2001 ◽  
Vol 10 (2) ◽  
pp. 213-240 ◽  
Author(s):  
Manying Ip Wardlow Friesen
Keyword(s):  
New Zealand ◽  
Return Migration ◽  
Host Society ◽  
Chinese Community ◽  
Republic Of China ◽  
Economic Motivation ◽  
Economic Miracle ◽  
Chinese Migrants ◽  
Depth Interviews

The new Chinese community in New Zealand (formed since 1987) is made up of immigrants from the People's Republic of China, Taiwan, Hong Kong and Malaysia as well as other countries. Initially looked upon as harbingers of the “Asian economic miracle” by the New Zealand government, the new arrivals met with numerous unforeseen difficulties. This article is based on the findings of surveys and in-depth interviews in which the primary migrants were asked about their motives for migration, the economic and social outcomes of their migration, their perception of the comparative strengths of their native land and New Zealand, and their long-term view on settlement and return migration. The surveys are also set against background statistics from the 1996 census as well as immigration figures up to 2000. The findings challenge the assumption of the importance of the economic motivation of migration, and point to the primacy of social and environmental factors. They also suggest that transnationalism is a long-term strategy, instead of a temporary expediency, but also that most Chinese migrants in New Zealand have tried to integrate with the host society when possible.

Harvest rate of sooty shearwaters (Puffinus griseus) by Rakiura Māori: a potential tool to monitor population trends?

2004 ◽  
Vol 31 (3) ◽  
pp. 319 ◽  
Author(s):  
Jane Catherine Kitson
Keyword(s):  
New Zealand ◽  
Cross Section ◽  
Large Scale ◽  
Population Change ◽  
Population Trends ◽  
Harvest Rate ◽  
General Index ◽  
Careful Screening ◽  
Potential Tool

Sooty shearwaters (tītī, muttonbird, Puffinus griseus) are highly abundant migratory seabirds, which return to breeding colonies in New Zealand. The Rakiura Māori annual chick harvest on islands adjacent to Rakiura (Stewart Island), is one of the last large-scale customary uses of native wildlife in New Zealand. This study aimed to establish whether the rate at which muttonbirders can extract chicks from their breeding burrows indicates population trends of sooty shearwaters. Harvest rates increased slightly with increasing chick densities on Putauhinu Island. Birders' harvest rates vary in their sensitivities to changing chick density. Therefore a monitoring panel requires careful screening to ensure that harvest rates of the birders selected are sensitive to chick density, and represents a cross-section of different islands. Though harvest rates can provide only a general index of population change, it can provide an inexpensive and feasible way to measure population trends. Detecting trends is the first step to assessing the long-term sustainability of the harvest.

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