scholarly journals Extraction of bioactive compounds from Psidium guajava and their application in dentistry

AMB Express ◽  
2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Shaik Shaheena ◽  
Anjani Devi Chintagunta ◽  
Vijaya Ramu Dirisala ◽  
N. S. Sampath Kumar

AbstractGuava is considered as poor man’s apple rich in phytochemicals with medicinal value and hence it is highly consumed. Gas chromatography–mass spectroscopy (GC–MS) analysis of guava leaf extract revealed the presence of various bioactive compounds with antimicrobial, antioxidant, anticancer, and antitumor properties. Hence, it is used in tooth paste formulations along with other ingredients such as Acacia arabica gum powder, stevia herb powder, sea salt, extra virgin coconut oil, peppermint oil in the present study. Three formulations F1, F2 and F3 have been made by varying the concentration of these ingredients and the prepared formulations were studied for their antimicrobial activity and physico-chemical parameters such as pH, abrasiveness, foaming activity, spreading and cleaning ability. Among these, F3 showed significant antioxidant and antimicrobial properties, minimal cytotoxicity, maximum spreadability and very high cleaning ability. This study surmises that the herbal toothpaste formulation is greener, rich in medicinal values and imparts oral hygiene.

2020 ◽  
Author(s):  
Sampath Kumar N.S. ◽  
Norizah Mhd Sarbon ◽  
Sandeep Singh Rana ◽  
Anjani Devi Chintagunta ◽  
Prathibha S ◽  
...  

Abstract Psidium guajava (guava) is predominantly grown in India and known for its medicinal properties in treating various diseases and disorders. The present work focuses on aqueous extraction of bioactive compounds from the guava leaf and its utilization in the formulation of jelly to improve the public health. The guava leaf extract has been used in the preparation of jelly with pectin (1.5 g), sugar (28 g) and lemon juice (2 mL). The prepared guava leaf extract jelly (GJ) and the control jelly (CJ, without extract) were subjected to proximate, nutritional and textural analyses besides determination of antioxidant and antimicrobial activities. GJ was found to contain carbohydrate (45.78 g/100 g), protein (3.0 g/100 g), vitamin C (6.15 mg/100 g), vitamin B3 (2.90 mg/100 g) and energy (120.6 kcal). Further, the texture analysis of CJ and GJ indicated that both the jellies showed similar properties emphasizing that the addition of guava leaf extract does not bring any change in the texture properties of jelly. GJ exhibited antimicrobial activity against various bacteria ranging from 11.4–13.6 mm. Similarly, GJ showed antioxidant activity of 42.38% against DPPH radical and 33.45% against hydroxyl radical. Mass spectroscopic analysis of aqueous extract confirmed the presence of esculin, quercetin, gallocatechin, 3-Sinapoylquinic acid, gallic acid, citric acid and ellagic acid which are responsible for antioxidant and antimicrobial properties.


AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
N. S. Sampath Kumar ◽  
Norizah Mhd Sarbon ◽  
Sandeep Singh Rana ◽  
Anjani Devi Chintagunta ◽  
S. Prathibha ◽  
...  

AbstractPsidium guajava L. (guava) is predominantly grown throughout the world and known for its medicinal properties in treating various diseases and disorders. The present work focuses on aqueous extraction of bioactive compounds from the guava leaf and its utilization in the formulation of jelly to improve the public health. The guava leaf extract has been used in the preparation of jelly with pectin (1.5 g), sugar (28 g) and lemon juice (2 mL). The prepared guava leaf extract jelly (GJ) and the control jelly (CJ, without extract) were subjected to proximate, nutritional and textural analyses besides determination of antioxidant and antimicrobial activities. GJ was found to contain carbohydrate (45.78 g/100 g), protein (3.0 g/100 g), vitamin C (6.15 mg/100 g), vitamin B3 (2.90 mg/100 g) and energy (120.6 kcal). Further, the texture analysis of CJ and GJ indicated that both the jellies showed similar properties emphasizing that the addition of guava leaf extract does not bring any change in the texture properties of jelly. GJ exhibited antimicrobial activity against various bacteria ranging from 11.4 to 13.6 mm. Similarly, GJ showed antioxidant activity of 42.38% against DPPH radical and 33.45% against hydroxyl radical. Mass spectroscopic analysis of aqueous extract confirmed the presence of esculin, quercetin, gallocatechin, 3-sinapoylquinic acid, gallic acid, citric acid and ellagic acid which are responsible for antioxidant and antimicrobial properties.


2020 ◽  
Vol 26 (5) ◽  
pp. 519-541 ◽  
Author(s):  
Giovanna Ferrentino ◽  
Ksenia Morozova ◽  
Christine Horn ◽  
Matteo Scampicchio

Background: The use of essential oils is receiving increasing attention worldwide, as these oils are good sources of several bioactive compounds. Nowadays essential oils are preferred over synthetic preservatives thanks to their antioxidant and antimicrobial properties. Several studies highlight the beneficial effect of essential oils extracted from medicinal plants to cure human diseases such as hypertension, diabetes, or obesity. However, to preserve their bioactivity, the use of appropriate extraction technologies is required. Method: The present review aims to describe the studies published so far on the essential oils focusing on their sources and chemical composition, the technologies used for their recovery and their application as antioxidants in food products. Results: The review has been structured in three parts. In the first part, the main compounds present in essential oils extracted from medicinal plants have been listed and described. In the second part, the most important technologies used for extraction and distillation, have been presented. In detail, conventional methods have been described and compared with innovative and green technologies. Finally, in the last part, the studies related to the application of essential oils as antioxidants in food products have been reviewed and the main findings discussed in detail. Conclusions: In summary, an overview of the aforementioned subjects is presented by discussing the results of the most recent published studies.


2019 ◽  
Vol 0 (0) ◽  
Author(s):  
Ashraf Farshbaf-Sadigh ◽  
Hoda Jafarizadeh-Malmiri ◽  
Navideh Anarjan ◽  
Yahya Najian

Abstract Ginger oil in water (O/W) nanoemulsions, were produced using phase inversion composition method and Tween 80, as emulsifier. Effects of processing parameters namely, stirring rate (100 to1000 rpm) and water addition rate (1–10 mL/min) were evaluated on the physico-chemical, morphological, antioxidant and antimicrobial properties of the prepared O/W nanoemulsions using response surface methodology (RSM). Results indicated that well dispersed and spherical ginger nanodroplets were formed in the nanoemulsions with minimum particle size (8.80 nm) and polydispersity index (PDI, 0.285) and maximum zeta potential value (−9.15 mV), using stirring rate and water addition rate of 736 rpm and 8.18 mL/min, respectively. Insignificant differences between predicted and experimental values of the response variables, indicated suitability of fitted models using RSM. Mean particle size of the prepared nanoemulsion using optimum conditions were changed from 8.81 ± 1 to 9.80 ± 1 nm, during 4 weeks of storage, which revealed high stability of the resulted ginger O/W nanoemulsion. High antioxidant activity (55.4%), bactericidal (against Streptococcus mutans) and fungicidal (against Aspergillus niger) activities of the prepared nanoemulsion could be related to the presence of gingerols and shogaols, a group of phenolic alkanones, in the ginger oil, which those were detected by gas chromatography method.


Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 325
Author(s):  
Nitin Chandra teja Dadi ◽  
Matúš Dohál ◽  
Veronika Medvecká ◽  
Juraj Bujdák ◽  
Kamila Koči ◽  
...  

This research was aimed at the preparation of a hybrid film based on a layered silicate saponite (Sap) with the immobilized photosensitizer phloxine B (PhB). Sap was selected because of its high cation exchange capacity, ability to exfoliate into nanolayers, and to modify different surfaces. The X-ray diffraction of the films confirmed the intercalation of both the surfactant and PhB molecules in the Sap film. The photosensitizer retained its photoactivity in the hybrid films, as shown by fluorescence spectra measurements. The water contact angles and the measurement of surface free energy demonstrated the hydrophilic nature of the hybrid films. Antimicrobial effectiveness, assessed by the photodynamic inactivation on hybrid films, was tested against a standard strain and against methicillin-resistant bacteria of Staphylococcus aureus (MRSA). One group of samples was irradiated (green LED light; 2.5 h) and compared to nonirradiated ones. S. aureus strains manifested a reduction in growth from 1-log10 to over 3-log10 compared to the control samples with Sap only, and defects in S. aureus cells were proven by scanning electron microscopy. The results proved the optimal photo-physical properties and anti-MRSA potential of this newly designed hybrid system that reflects recent progress in the modification of surfaces for various medical applications.


Coatings ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 939
Author(s):  
Prabhuraj D. Venkatraman ◽  
Usha Sayed ◽  
Sneha Parte ◽  
Swati Korgaonkar

The development of textile finishing with improved functional properties has been a growing interest among industry and scientists worldwide. The recent global pandemic also enhanced the awareness amongst many toward improved hygiene and the use of antimicrobial textiles. Generally, natural herbal components are known to possess antimicrobial properties which are green and eco-friendly. This research reports a novel and innovative method of developing and optimising nano-emulsions using two combinations of herbal extracts produced from Moringa oleifera, curry leaf, coconut oil (nano-emulsion 1) and other using Aegle marmelos with curry leaf and coconut oil (nano-emulsion 2). Nano-emulsions were optimised for their pH, thermal stability, and particle size, and percentage add-on. Organic cotton fabrics (20 and 60 gsm) were finished with nano-emulsions using continuous and batch processes and characterised for their surface morphology using scanning electron microscopy, energy dispersive X-ray (EDX) analysis and Fourier transform infrared spectroscopy (FTIR) analysis. The finished fabrics were evaluated for their Whiteness Index, assessed for antimicrobial resistance against Gram-positive (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli) using AATCC 100 and 147 methods. In addition, fabrics were assessed for their antifungal efficacy (AATCC 30), tensile strength and air permeability. Results suggested that finished organic fabrics with nano-emulsions had antimicrobial resistance, antifungal, wash fastness after 20 washing cycles, and sufficient strength. This novel finishing method suggests that organic cotton fabrics treated with nano-emulsions can be used as a durable antimicrobial textile for healthcare and hygiene textiles.


Food Research ◽  
2020 ◽  
Vol 4 (6) ◽  
pp. 1937-1946
Author(s):  
M.M.D.R. Tugay ◽  
L.E. Mopera ◽  
E.B. Esguerra ◽  
K.A.T. Castillo-Israel

This study aimed to characterize and compare pili (Canarium ovatum Engl.) pulp oil from two different varieties of pili fruits in Bicol, Philippines namely M. Orolfo and Orbase varieties for possible utilization into oil-based products. The effects of varietal differences in pili fruits on physical, chemical and quality characteristics of its pulp oil were determined. These two oils were also compared with control oils, commercially available coconut oil and extra virgin olive oil. Pili pulp oil from M. Orolfo had dark color while Orbase had color close to extra virgin olive oil. The two varieties did not significantly differ from each other in terms of refractive index, moisture and volatile matters, acid value, iodine value, saponification number and percent unsaponifiable matter but significantly differed from coconut oil and extra virgin olive oil. On the other hand, the two varieties significantly differed from each other in terms of peroxide value, Vitamin A and α-tocopherol contents. In terms of fatty acid profile, high amounts of palmitic acid were determined in both pili varieties (19-25%) compared with coconut oil (6.34%). Oleic acid in Orbase was 71.5% while M. Orolfo had 58.1%, which are comparable with extra virgin olive oil (77.9%). Pili pulp oils from M. Orolfo and Orbase can be utilized into oilbased products because its chemical and quality characteristics are within the standard. Both can be stored for a longer period of time and healthier in terms of fatty acid composition and natural antioxidant content.


Author(s):  
Victoriţa BONTA ◽  
Daniel Severus DEZMIREAN ◽  
Liviu Alexandru MĂRGHITAŞ ◽  
Adriana Cristina URCAN ◽  
Otilia BOBIŞ

Honey is used for centuries both as nutrient and in medicine, being an exhaustless source of benefic factors for health and also for different biotechnological processes. This is a complex matrix which possesses antioxidant and antimicrobial properties due to its peroxidic activity and bioactive compounds. A fully validated approach for the determination of sugars, hydroxymethylfurfural content and diastase activity in honey samples were presented in accordance with the official methods from the International Honey Commission. Spectrophotometric method was used for diastazic activity determination and chromatographic determinations for HMF content (photodiode array detection) and sugars (refractive index detection). The limits of detection and quantification were calculated. Accuracy, precision and uncertainty were determined in the spectrophotometric and chromatographic techniques using the certified reference material. Diastase activity, sucrose content and hydroxymethylfurfural (HMF) content were easily differentiated and these parameters were used for indication of a possible adulteration of the honey. The present study confirms that, in order to have an authentic honey sample, diastazic activity must be above 8 DN (diastazic number) and HMF content less than 40 mg/kg. Sugar spectrum is different for every honey type. Consequently, physico-chemical analysis should be combined for the determination of authentic honey and proper labeling.


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