scholarly journals TLR7/8 signalling affects X-sperm motility via the GSK3 α/β-hexokinase pathway for the efficient production of sexed dairy goat embryos

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Fa Ren ◽  
Huaming Xi ◽  
Yijie Ren ◽  
Yu Li ◽  
Fei Wen ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Sperm Motility ◽  
Glycogen Synthase ◽  
Lower Layer ◽  
Dairy Goat ◽  
In Vitro Fertilisation ◽  
Mitochondrial Activity ◽  
Efficient Production ◽  
Dairy Goats ◽  
Offspring Sex Ratio

Abstract Background Goat milk is very similar to human milk in terms of its abundant nutrients and ease of digestion. To derive greater economic benefit, farmers require more female offspring (does); however, the buck-to-doe offspring sex ratio is approximately 50%. At present, artificial insemination after the separation of X/Y sperm using flow cytometry is the primary means of controlling the sex of livestock offspring. However, flow cytometry has not been successfully utilised for the separation of X/Y sperm aimed at sexing control in dairy goats. Results In this study, a novel, simple goat sperm sexing technology that activates the toll-like receptor 7/8 (TLR7/8), thereby inhibiting X-sperm motility, was investigated. Our results showed that the TLR7/8 coding goat X-chromosome was expressed in approximately 50% of round spermatids in the testis and sperm, as measured from cross-sections of the epididymis and ejaculate, respectively. Importantly, TLR7/8 was located at the tail of the X-sperm. Upon TLR7/8 activation, phosphorylated forms of glycogen synthase kinase α/β (GSK3 α/β) and nuclear factor kappa-B (NF-κB) were detected in the X-sperm, causing reduced mitochondrial activity, ATP levels, and sperm motility. High-motility Y-sperm segregated to the upper layer and the low-motility X-sperm, to the lower layer. Following in vitro fertilisation using the TLR7/8-activated sperm from the lower layer, 80.52 ± 6.75% of the embryos were XX females. The TLR7/8-activated sperm were subsequently used for in vivo embryo production via the superovulatory response; nine embryos were collected from the uterus of two does that conceived. Eight of these were XX embryos, and one was an XY embryo. Conclusions Our study reveals a novel TLR7/8 signalling mechanism that affects X-sperm motility via the GSK3 α/β-hexokinase pathway; this technique could be used to facilitate the efficient production of sexed dairy goat embryos.

scholarly journals TLR7/8 Signalling Affects X-Sperm Motility via the GSK3 α/β-Hexokinase Pathway for the Efficient Production of Sexed Dairy Goat Embryos

2021 ◽  
Author(s):  
Fa Ren ◽  
Huaming Xi ◽  
Yijie Ren ◽  
Yu Li ◽  
Fei Wen ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Sperm Motility ◽  
Cross Sections ◽  
Glycogen Synthase ◽  
Lower Layer ◽  
Economic Benefits ◽  
Dairy Goat ◽  
Mitochondrial Activity ◽  
Efficient Production

Abstract BackgroundGoat milk is most similar to human milk because of its abundant nutrients and ease of digestion. To derive more economic benefits, farmers need to obtain offspring with more does; however, the buck to doe sex ratio of offspring is approximately 50%. At present, artificial insemination after separation of X/Y sperm using flow cytometry is the primary means to control the sex of livestock offspring. However, flow cytometry has not been successful in separating X/Y sperm for sexing control in dairy goats.ResultsIn this study, a novel, simple goat sperm-sexing technology that activates the toll-like receptor 7/8 (TLR7/8), inhibiting X-sperm motility, was investigated. Results showed that TLR7/8 coding goat X-chromosome was expressed in approximately 50% of round spermatids in testis' cross-sections and sperm in the epididymis' cross-sections and ejaculate. Importantly, TLR7/8 was located at the tail of X-sperm. Upon TLR7/8 activation, phosphorylated forms of glycogen synthase kinase α/β (GSK3 α/β) and nuclear factor kappa-B (NFκB) were detected in the X-sperm, reducing mitochondrial activity, ATP levels, and sperm motility. High-motility Y-sperm segregated to the upper layer and low-motility X-sperm to the lower layer. Following in vitro fertilisation using the lower layer TLR7/8-activated sperm, 80.52 ± 6.75% of the embryos were XX females. TLR7/8-activated sperm was used for in vivo embryo production via the superovulatory response, and 88.89% of the embryos were XX females.ConclusionsOur study reveals a novel TLR7/8 signalling mechanism that affects X-sperm motility via the GSK3 α/β-hexokinase pathway, facilitating the efficient production of sexed dairy goat embryos.

scholarly journals Mitochondrial enrichment in infertile patients: a review of different mitochondrial replacement therapies

2021 ◽  
Vol 15 ◽  
pp. 263349412110235
Author(s):  
Cristina Rodríguez-Varela ◽  
Sonia Herraiz ◽  
Elena Labarta
Keyword(s):  
Stem Cells ◽  
Genetic Material ◽  
External Source ◽  
Oocyte Quality ◽  
Third Party ◽  
In Vitro Fertilisation ◽  
Mitochondrial Activity ◽  
Mitochondrial Transfer ◽  
Infertile Patients

Poor ovarian responders exhibit a quantitative reduction in their follicular pool, and most cases are also associated with poor oocyte quality due to patient’s age, which leads to impaired in vitro fertilisation outcomes. In particular, poor oocyte quality has been related to mitochondrial dysfunction and/or low mitochondrial count as these organelles are crucial in many essential oocyte processes. Therefore, mitochondrial enrichment has been proposed as a potential therapy option in infertile patients to improve oocyte quality and subsequent in vitro fertilisation outcomes. Nowadays, different options are available for mitochondrial enrichment treatments that are encompassed in two main approaches: heterologous and autologous. In the heterologous approach, mitochondria come from an external source, which is an oocyte donor. These techniques include transferring either a portion of the donor’s oocyte cytoplasm to the recipient oocyte or nuclear material from the patient to the donor’s oocyte. In any case, this approach entails many ethical and safety concerns that mainly arise from the uncertain degree of mitochondrial heteroplasmy deriving from it. Thus the autologous approach is considered a suitable potential tool to improve oocyte quality by overcoming the heteroplasmy issue. Autologous mitochondrial transfer, however, has not yielded as many beneficial outcomes as initially expected. Proposed mitochondrial autologous sources include immature oocytes, granulosa cells, germline stem cells, and adipose-derived stem cells. Presently, it would seem that these autologous techniques do not improve clinical outcomes in human infertile patients. However, further trials still need to be performed to confirm these results. Besides these two main categories, new strategies have arisen for oocyte rejuvenation by improving patient’s own mitochondrial function and avoiding the unknown consequences of third-party genetic material. This is the case of antioxidants, which may enhance mitochondrial activity by counteracting and/or preventing oxidative stress damage. Among others, coenzyme-Q10 and melatonin have shown promising results in low-prognosis infertile patients, although further randomised clinical trials are still necessary.

Reasons for keeping dairy goats in Tanzania, and possible goals for a sustainable breeding program

2017 ◽  
Vol 57 (2) ◽  
pp. 338 ◽  
Author(s):  
Z. C. Nziku ◽  
G. C. Kifaro ◽  
L. O. Eik ◽  
T. Steine ◽  
T. Ådnøy
Keyword(s):  
Milk Yield ◽  
Production Systems ◽  
Holistic Approach ◽  
Breeding Program ◽  
Dairy Goat ◽  
Dairy Goats ◽  
Quantitative And Qualitative Research ◽  
Animal Identification ◽  
Breeding Goals ◽  
And Performance

This research aimed at describing reasons for keeping dairy goats in Tanzania, and possible goals for a sustainable breeding program. Three districts, each representing a unique dairy goat breed population, were selected for the study. The Saanen, Toggenburg and Norwegian were the main dairy goat breeds in Arumeru, Babati, and Mvomero districts, respectively. A total of 125 dairy goat farmers were interviewed. A holistic approach of both quantitative and qualitative research methods was used to study the perceptions of farmers. More milk yield, sale of breeding stock and manure were the highest ranked reasons for keeping dairy goats. The reasons were coherent to the production systems. The three most preferred traits for improvement were milk yield, adaptability and twinning ability. These preferences were absolutely important in the context of the referred production system. Selection of replacement stock, animal identification and performance recording were the main challenges emphasised by farmers. The present study views these challenges as a result of knowledge gaps in animal breeding that require solutions. Based on result findings it is suggested that the milk yield and survival traits should be the primary dairy goat breeding goals. Generally, there are possibilities for developing sustainable dairy goat breeding programs in the surveyed areas given relevant breeding goals are incorporated. The design of simple and manageable dairy goat breeding schemes is necessary.

scholarly journals Spatialization of climate, physical and socioeconomic factors that affect the dairy goat production in Brazil and their impact on animal breeding decisions

2012 ◽  
Vol 32 (11) ◽  
pp. 1073-1081 ◽  
Author(s):  
Fernando B. Lopes ◽  
Marcelo C. da Silva ◽  
Eliane S. Miyagi ◽  
Maria C.S. Fioravanti ◽  
Olivardo Facó ◽  
...  
Keyword(s):  
Vegetation Index ◽  
Normalized Difference Vegetation Index ◽  
Animal Breeding ◽  
Dairy Goat ◽  
Dairy Goats ◽  
Family Farming ◽  
Breeding Programs ◽  
Environmental Diversity ◽  
Political Realities ◽  
Technical Guidance

Brazil has high climate, soil and environmental diversity, as well as distinct socioeconomic and political realities, what results in differences among the political administrative regions of the country. The objective of this study was to determine spatial distribution of the physical, climatic and socioeconomic aspects that best characterize the production of dairy goats in Brazil. Production indices of milk per goat, goat production, milk production, as well as temperature range, mean temperature, precipitation, normalized difference vegetation index, relative humidity, altitude, agricultural farms; farms with native pasture, farms with good quality pasture, farms with water resources, farms that receive technical guidance, family farming properties, non-familiar farms and the human development index were evaluated. The multivariate analyses were carried out to spatialize climatic, physical and socioeconomic variables and so differenciate the Brazilian States and Regions. The highest yields of milk and goat production were observed in the Northeast. The Southeast Region had the second highest production of milk, followed by the South, Midwest and North. Multivariate analysis revealed distinctions between clusters of political-administrative regions of Brazil. The climatic variables were most important to discriminate between regions of Brazil. Therefore, it is necessary to implement animal breeding programs to meet the needs of each region.

scholarly journals Analisis Faktor-Faktor Yang Mempengaruhi Produksi Susu Kambing Perah Peranakan Etawa (PE) di Desa Limpok Kecamatan Darussalam Kabupaten Aceh Besar

2017 ◽  
Vol 2 (1) ◽  
pp. 127-133
Author(s):  
Rini Afrida ◽  
Edy Marsudi ◽  
Ismayani Ismayani
Keyword(s):  
Milk Production ◽  
Goat Milk ◽  
Dairy Goat ◽  
Case Study Method ◽  
Dairy Goats ◽  
Factors Affecting ◽  
Livestock Farm ◽  
Simultaneous Test ◽  
Object Of Study

ANALISIS FAKTOR-FAKTOR YANG MEMPENGARUHI PRODUKSI SUSU KAMBING PERAH PERANAKAN ETAWA (PE) DI DESA LIMPOK KECAMATAN DARUSSALAM KABUPATEN ACEH BESAR (STUDI KASUS DI UD. ATJEH LIVESTOCK FARM) OLEH Rini Afrida / Agribisnis Universitas Syiah Kuala  ABSTRAK Susu kambing perah merupakan komoditas yang cukup penting di Indonesia. Penelitian ini bertujuan untuk  Menganalisis pengaruh jumlah pemberian pakan hijauan dan  ampas tahu terhadap  produksi susu kambing perah di UD. Atjeh Livestock Farm yang berada  di Desa Limpok Kecamatan Darussalam Kabupaten Aceh Besar. Metode penelitian yang digunakan adalah metode studi kasus dengan objek penelitian seluruh kambing perah peranakan etawa (PE) yang memproduksi susu di UD. Atjeh Livestock Farm. Metode analisis yang digunakan dalam penelitian ini adalah Model Cobb Douglas. Hasil penelitian menunjukkan bahwa variabel jumlah pakan hijauan dan ampas tahu mempunyai pengaruh nyata baik secara  serempak  maupun parsial terhadap produksi   susu, dimana diperoleh nilai dari uji serempak untuk   Fhit = 138.415 sedangkan untuk Ftabel = 3.98, karena nilai Fhit = 138.415   Ftabel = 3.98, maka kesimpulan yang dapat diambil adalah menolak H0. Artinya variabel hijauan  dan ampas tahu secara bersama – sama mempunyai pengaruh nyata terhadap produksi susu kambing perah, sedangkan dari uji parsial diperoleh nilai variabel hijauan untuk thitung = 4,016 sedangkan ttabelnya = 2,17881, karena nilai thitung= 4,016   ttabel = 2,17881, maka kesimpulan yang dapat diambil adalah menolak H0. Begitu juga untuk  variabel ampas tahu  diperoleh nilai untuk thitung =  2,255 sedangkan ttabelnya = 2,17881, karena nilai thitung = 2,255   ttabel = 2,17881, maka kesimpulan yang dapat diambil adalah menolak H0. Artinya  variabel hijauan maupun ampas tahu mempunyai pengaruh nyata terhadap produksi susu kambing perah.ANALYSIS OF THE FACTORS AFFECTING THE PRODUCTION OF GOAT MILK DAIRY CROSSBREED ETAWA (PE) IN THE LIMPOK VILLAGE SUB-DISTRICTS OF DARUSSALAM ACEH BESAR REGENCY(A Case Study in UD. Atjeh Livestock Farm)ABSTRACT Dairy goat milk is a commodity that is quite important in Indonesia. This study aimed to analyze the influence of the number of feeding forage and pulp out of the milk production of dairy goats in UD. Atjeh Livestock Farm that are in the Limpok village sub-district of Darussalam Aceh Besar regency. The method used is the case study method with the object of study throughout the dairy goat hybrid etawa (PE), which produces milk in UD. Atjeh Livestock Farm. The analytical method used in this study is a model Cobb Douglas. The results showed that the variable amount of green feed and tofu has a real influence both simultaneously and partially to the production of milk, which gained the value of simultaneous test for Fcount = 138.415 whereas for Ftable = 3.98, because the value Fcount = 138.415   Ftable = 3.98, the conclusions that can be drawn is to reject H 0. This means that the variable forages and pulp out together have a significant effect on the milk production of dairy goats, whereas the values obtained from the partial test variable forage for tcount = 4,016 whereas ttable = 2,17881, because the value of tcount= 4,016   ttable = 2,17881, the conclusions that can be drawn is to reject H 0. Likewise for variable pulp out obtained value for tcount =  2,255 whereas ttable  = 2,17881, because the value of tcount = 2,255   ttable = 2,17881, the conclusions that can be drawn is to reject H 0. This means that the variable forage or pulp out has a significant effect on the milk production of dairy goats.  

scholarly journals Botanical and mineral composition of dominant wild forages for feeding of dairy goats in Payakumbuh Region

2021 ◽  
Vol 888 (1) ◽  
pp. 012063
Author(s):  
Khalil ◽  
Imana Martaguri
Keyword(s):  
Mineral Composition ◽  
Block Design ◽  
Dairy Goat ◽  
Panicum Maximum ◽  
Dairy Goats ◽  
Randomized Design ◽  
Random Block ◽  
Field Edge ◽  
Random Block Design ◽  
Highly Correlated

Abstract The objectives of this study were (i) to identify the diversity of forages that are usually consumed or fed to the dairy goats, (ii) to assess the mineral composition of dominant forages concerning the soil mineral status of growing areas. Eight dairy goat farms surveyed and collected forage samples to define the diversity and dominant forages used to feed the goat. Samples of three dominant forages (Asystasia gangetica, Axonopus compressus, and Panicum maximum) and soils were then collected in five different areas where the farmers usually derived the forages (banana plantation, idle lands, riverbanks, roadside, and rice field edge). Plant samples were collected by using a plate meter used for the calculation of botanical composition. Fresh samples were then chopped, dried, and then ground in meal form before analysis for dry matter (DM), and minerals of Ca, P, Mg, K, Na, and S. The same minerals were analyzed for soil samples. Data were subjected to analysis of variance (ANOVA) in a completely randomized design of 5x3 consisting of 3 plant species and five growing areas as replications. Data were statistically analyzed using correlation and variance analysis in Random Block Design (RBD). Results showed of the total about 45 types of plants commonly consumed by goats, there were three dominant species, i.e., P. maximum (38.60%), A. gangetica (24.23%), and A. compressus (17.23%). The Ca content of forages highly correlated with the Ca in the soils, while magnesium in the forage was negatively correlated with the magnesium in the soil. It was concluded that the dominant forages were found a good source of Ca and Mg but deficient phosphorus.

Effect of mid-line or low-line milking systems on lipolysis and milk composition in dairy goats

2018 ◽  
Vol 156 (6) ◽  
pp. 848-854
Author(s):  
M. C. Beltrán ◽  
A. Manzur ◽  
M. Rodríguez ◽  
J. R. Díaz ◽  
C. Peris
Keyword(s):  
Fatty Acid ◽  
Free Fatty Acid ◽  
Milk Composition ◽  
Crossover Design ◽  
Dairy Goat ◽  
Dairy Goats ◽  
Milk Samples ◽  
Commercial Farms ◽  
Milk Storage ◽  
Enzymatic Coagulation

AbstractTwo experiments were carried out to investigate how milking in mid-line (ML) affects the lipolysis level and milk composition in goat livestock, in comparison with low-line (LL) milking. The first experiment took place, in triplicate, on an experimental farm. For each replicate, a crossover design (62 goats, two treatments, ML and LL, in two periods each lasting 4 days) was used. Milk samples were taken daily at 0 and 24 h after milking. In the first experimental replicate, some enzymatic coagulation cheeses were made, which were assessed by a panel of tasters at 50 and 100 days of maturation. In the second experiment, the lipolysis level and composition of tank milk from 55 commercial dairy goat farms (25 ML and 30 LL) were analysed, in milk samples taken in three different weeks. The results of the first experiment showed that ML milking increased free fatty acid (FFA) concentration in raw goat's milk significantly (0.71 v. 0.40 mmol/l, respectively). However, in the milk samples taken from commercial farms the FFA concentration remained unaffected by the milking pipeline height (0.59 v. 0.58 mmol/l for ML and LL, respectively). No significant differences were found in the milk composition, nor in the sensory characteristics in the cured cheeses, which suggests that factors other than the milkline height are able to influence the level of lipolysis under commercial conditions. Therefore, ML milking should not be discouraged, provided that the correct functioning and management of the milking operation and milk storage on the farm is guaranteed.

Effects of subacute ruminal acidosis on colon epithelial morphological structure, permeability and expression of key tight junction proteins in a dairy goat model

2020 ◽  
Author(s):  
Mengya wang ◽  
Yang Li ◽  
Min Gao ◽  
Liwen Song ◽  
Ming Xu ◽  
...  
Keyword(s):  
Tight Junction ◽  
Barrier Function ◽  
Morphological Structure ◽  
Neutral Detergent Fiber ◽  
Dairy Goat ◽  
Epithelial Barrier ◽  
Colonic Epithelium ◽  
Dairy Goats ◽  
Subacute Ruminal Acidosis ◽  
Ruminal Acidosis

Abstract Background: The hindgut epithelial barrier plays an important role in maintaining absorption and immune homeosrasis in ruminants. However, there is little information available on changes of colon epithelial barrier structure and function following grain-induced subacute ruminal acidosis (SARA). The objective of this study was to investigate the effects of SARA on colon epithelial morphological structure, permeability and gene expression involved in epithelial barrier function using dairy goats as a ruminant model. Methods: Twelve mid-lactating Sannan dairy goats (62.13 ± 4.76 kg) were randomly divided into either control (n = 6) or SARA treatment (n = 6). Control goats were fed a mixed diet (non-fiber carbohydrates/neutral detergent fiber (NFC/NDF) =1.15) for 60 days, SARA induction goats were given 4 mixed diets with NFC to NDF ratios of 1.15, 1.49, 2.12 and 2.66 to induce development of SARA. The duration of each diet was 15 d including 12 d for adaption and 3 d for sampling. Continuous ruminal pH recordings were utilized to diagnose the severity of SARA. Additionally, Plasma and colonic tissue were collected to evaluate the epithelial tissue morphological structure, permeability and expression of tight junction (TJ) protein using histological techniques, Ussing chamber, real-time PCR and western blotting. Results: Profound disruption in the colonic epithelium was mainly manifested as the electron density of TJ decreased, intercellular space widened and mitochondria swelled in SARA dairy goats. Epithelial Isc, Gt and the mucosal-to-serosal flux of FD4 ( P < 0.05) were increased, PD was decreased in SARA dairy goats compared with the control. SARA increased the plasma LPS ( P < 0.001), D-lactic acid contents ( P = 0.025) and DAO activity ( P = 0.003). Increased gene and protein expression of claudin-1 and occludin ( P < 0.05) were observed in colonic epithelium of SARA goats. Conclusion: Overall, the data of present study demonstrate that SARA can impair the barrier function of the colonic epithelium in dairy goats, which is associated with severe epithelial structural damage and increased permeability and changes in the expression of TJ proteins.

scholarly journals Incidence, possible risk factors and therapies for pseudopregnancy on Dutch dairy goat farms: a cross-sectional study

2019 ◽  
Vol 184 (25) ◽  
pp. 770-770 ◽  
Author(s):  
René Van den Brom ◽  
Rianne Klerx ◽  
Piet Vellema ◽  
Karianne Lievaart-Peterson ◽  
Jan Willem Hesselink ◽  
...  
Keyword(s):  
Risk Factors ◽  
Response Rate ◽  
Cross Sectional Study ◽  
Annual Incidence ◽  
Dairy Goat ◽  
Induction Treatment ◽  
Sectional Study ◽  
Dairy Goats ◽  
Cross Sectional ◽  
Treatment Measures

Pseudopregnancy is a frequently diagnosed reproductive disorder in (dairy) goats. This cross-sectional study evaluates the incidence, possible risk factors and therapies for pseudopregnancy on Dutch dairy goat farms. Two questionnaires, one for farmers and one for veterinarians, were designed and included questions about general farm demographics, breeding management, hormonal oestrous induction, treatment, measures for reduction and stress moments in dairy goats in the period June 1, 2016–May 31, 2017. In total, 43 farmers (21.5 per cent response rate) and 27 veterinarians (22.5 per cent response rate) completed the questionnaire. The annual incidence of pseudopregnancy varied between 1 and 54 per cent per farm, with a mean annual incidence of 17 per cent (95 per cent CI 0.14 to 0.21). In this study, we found a significant association between incidence of pseudopregnancy and a higher percentage of goats with an extended lactation (p<0.0001) and between incidence of pseudopregnancy and the number of ultrasound examinations per year (p<0.0001). The recommended therapy in literature consists of two administrations of prostaglandins. This was only correctly applied by 10 per cent of the farms. On 52 per cent of the farms, an overdose was used comparing to the recommended dose in literature.

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